NKT cell-based immunotherapy for lung cancer

• Project/Area Number: 19591609
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Chiba University
• Principal Investigator: MOTOHASHI Shinichiro Chiba University, 大学院・医学研究院, 准教授 (60345022)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: NKT 細胞 / 免疫細胞療法 / 肺癌 / NKT細胞

Research Abstract

In vivoでのNKT 細胞活性化を目的としたaガラクトシルセラミドパルス樹状細胞(DC)療法後の腫瘍局所におけるNKT 細胞免疫系を解析した結果、DC 投与により活性化したNKT 細胞の腫瘍への浸潤が明らかに増強していることが判明した。さらにNKT 細胞免疫療法施行症例の末梢血を用いてcDNA microarray による遺伝子発現解析を行い、免疫反応の増強が認められた症例に特異的な複数の遺伝子発現変化を認めた。

Research Products

• [Journal Article] Combination therapy of in vitro-expanded natural killer T cells and α-galactosylceramide-pulsed antigenpresenting cells in patients with recurrenthead and neck carcinoma (2009)
  • Author(s): Kunii, N., Horiguchi, S., Motohashi, S.,Yamamoto, H., Ueno, N., Yamamoto, S.,Sakurai, D., Taniguchi, M., Nakayama, T., and Okamoto, Ya
  • Journal Title: Cancer Sci. (in press)
  • Peer Reviewed
• [Journal Article] Invariantnatural killer T cell-based immunotherapy forcancer (2009)
  • Author(s): Motohashi, S., and Nakayama, T
  • Journal Title: Immunotherapy 1 Pages: 73-82
  • Peer Reviewed
• [Journal Article] Naturalkiller T cell-mediated immunotherapy formalignant diseases (2009)
  • Author(s): Motohashi, S., and Nakayama, T
  • Journal Title: Frontiers in BioscienceS 1
  • Peer Reviewed
• [Journal Article] A phase I-II study of-Galactosylceramide-pulsedIL-2/GM-CSF-cultured peripheral bloodmononuclear cells in patients with advancedand recurrent non-small cell lung cancer (2009)
  • Author(s): Motohashi, S., Nagato, K., Kunii, N.,Yamamoto, H., Yamasaki, K., Okita, K.,Hanaoka, H., Shimizu, N., Suzuki, M.,Yoshino, I., Taniguchi, M., Fujisawa, T., and Nakayama, T
  • Journal Title: J.Immunol. 182(4) Pages: 2492-501
  • Peer Reviewed
• [Journal Article] A phase I-II study of α-galactosylceramide(KRN7000)-pulsed IL-2/G M-CFS-cultured peripheral blood mononuclear cells in patients wit advanced and recurrent non-small cell lung cancer. (2009)
  • Author(s): Motohashi, S.
  • Journal Title: J. Immunol. 182(4) Pages: 2492-2501
  • Peer Reviewed
• [Journal Article] Invariant natural killer T cell-based immunotherapy for cancer (2009)
  • Author(s): Motohashi, S.
  • Journal Title: Immunotherapy 1 Pages: 73-82
  • NAID: 10030922502
  • Peer Reviewed
• [Journal Article] INatural killer T cell-based immunotherapy for mailgnant diseases/ (2009)
  • Author(s): Motohashi, S.
  • Journal Title: Frontiers in Biocience S1 Pages: 108-116
  • Peer Reviewed
• [Journal Article] Human Th1differentiation induced bylipoarabinomannan/lipomannan fromMycobacterium bovis BCG Tokyo-172 (2008)
  • Author(s): Ito, T., Hasegawa, A., Hosokawa, H.,Yamashita, M., Motohashi, S., Naka, T.,Okamoto, Y., Fujita, Y., Ishii, Y., Taniguchi,M., Yano, I., and Nakayama, T
  • Journal Title: Int.Immunol 20(7) Pages: 849-860
  • Peer Reviewed
• [Journal Article] Aberrantmethylation of CXCL12 in non-small celllung cancer is associated with an unfavorableprognosis (2008)
  • Author(s): Suzuki, M., Mohamed, S., Nakajima, T.,Kubo, R., Tian, L., Fujiwara, T., Suzuki, H.,Nagato, K., Chiyo, M., Motohashi, S.,Yasufuku, K., Iyoda, A., Yoshida, S., Sekine,Y., Shibuya, K., Hiroshima, K., Nakatani, Y.,Yoshino, I., Fujisawa, T
  • Journal Title: Int. J. Oncol 33 Pages: 113-9
  • Peer Reviewed
• [Journal Article] Gfi1-mediated stabilization of GATA3protein is required for Th2 celldifferentiation (2008)
  • Author(s): Shinnakasu, R., Yamashita, M., Kuwahara,M., Hosokawa, H., Hasegawa, A.,Motohashi, S., Nakayama, T
  • Journal Title: J Biol Chem
  • Peer Reviewed
• [Journal Article] Phase I study ofα-galactosylceramide-pulsed antigenpresenting cells administration to thenasal submucosa in unresectable orrecurrent head and neck cancer (2008)
  • Author(s): Uchida, T., Horiguchi, S, Tanaka, Y.,Yamamoto, H., Kunii, N., Motohashi,S.,Taniguchi, M., Nakayama, T.,Okamoto,Y
  • Journal Title: CancerImmunol Immunother 57(3) Pages: 337-337
  • Peer Reviewed
• [Journal Article] Bmi1 regulates memory CD4T cell survival via repression of the Noxagene (2008)
  • Author(s): Yamashita, M., Kuwahara, M., Suzuki, A.,Hirahara, K., Shinnakasu, R., Hosokawa, H.,Hasegawa, A., Motohashi, S., Iwama, A., and Nakayama, T
  • Journal Title: J. Exp. Med 205 Pages: 110-110
  • Peer Reviewed
• [Journal Article] Clinicalapplications of natural killer T cell-basedimmunotherapy for cance (2008)
  • Author(s): Motohashi, S., and Nakayama, T
  • Journal Title: Cancer Sci 99 Pages: 638-645
  • Peer Reviewed
• [Journal Article] Clinical applications of natural killer T cell-based immunotherapy (2008)
  • Author(s): Motohashi, S.
  • Journal Title: Cancer Sci. 99(4) Pages: 638-645
  • Peer Reviewed
• [Journal Article] Human Th1 differentiation induced by lipoarabinomannan/lipomannan from Mycobacterium BCG Tokyo-172. (2008)
  • Author(s): Ito, T.
  • Journal Title: Int. Immunol 20(7) Pages: 849-860
  • Peer Reviewed
• [Journal Article] Gfi1-mediated stabilization of GATA3 protein is required for Th2 cell differentiation. (2008)
  • Author(s): Shinnakasu, R.
  • Journal Title: J. Biol. Chem. 283(42) Pages: 28216-28225
  • Peer Reviewed
• [Journal Article] Aberrant methylation of CXCL12 in non-small cell lung cancer is associated with an unfavorable prognosis. (2008)
  • Author(s): Suzuki, M.
  • Journal Title: Int. J. Oncol 33(1) Pages: 113-119
  • Peer Reviewed
• [Journal Article] Bmi1 regulates memory CD4 T cell sirvival via repression of the anoxa gene. (2008)
  • Author(s): Yamashita, M.
  • Journal Title: J. Exp. Med. 205(5) Pages: 1109-1120
  • Peer Reviewed
• [Journal Article] Phase l study of α-Galactosylceramide-pulsed anitgen pressenting cells administration to the nasal submucosa in unresectable or recurrent head and neck cancer (2008)
  • Author(s): Uchida, T.
  • Journal Title: Caner lmmunol lmmunother 57(3) Pages: 337-345
  • Peer Reviewed
• [Journal Article] Synchronousalterations of Wnt and epidermal growthfactor receptor signaling pathways throughaberrant methylation and mutation in nonsmall cell lung cancer (2007)
  • Author(s): Suzuki, M., Shigematsu, H., Nakajima T.,Kubo, R., Motohashi, S., Sekine, Y., Shibuya,K., Iizasa, T., Hiroshima, K., Nakatani, Y.,Gazdar, AF., Fujisawa, T
  • Journal Title: Clin Cancer Res 13(20) Pages: 6087-92
  • Peer Reviewed
• [Journal Article] Combination therapy of in vitro expanded natural killer T cells and α-galactosylceramide-pulses antigen presenting cells in patients wit recurrent head and neck carcinoma.
  • Author(s): Kunii, N.
  • Journal Title: Cancer Sci. (In press)
  • Peer Reviewed
• [Presentation] A Phase I-II study of αGalCel-pulsedantigen presenting cells in patients withadvanced or recurrent non-small cell lungcancer (2009)
  • Author(s): Motohashi, S
  • Organizer: The 5th International Symposium onCD1/NKT Cells
  • Place of Presentation: 鎌倉
  • Year and Date: 2009-03-29
• [Presentation] cells after preoperativea-Galactosylceramide-pulsed dendritic celltreatment. (2009)
  • Author(s): 本橋新一郎
  • Organizer: 千葉大学G-COE シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2009-01-06
• [Presentation] がん治療に向けたアフェレーシス産物から樹状細胞ならびにNKT細胞の誘導 (2008)
  • Author(s): Shinichiro Motohashi
  • Organizer: 第29回日本アフェレシス学会学術大会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2008-11-23
• [Presentation] NKT細胞免疫系を用いた肺癌に対する免疫細胞療法 (2008)
  • Author(s): Shinichiro Motohashi
  • Organizer: 第49回日本肺癌学会総会
  • Place of Presentation: 西日本総合展示場
  • Year and Date: 2008-11-14
• [Presentation] 原発性肺癌に対するNKT細胞免疫系を標とした免疫細胞療法 (2008)
  • Author(s): 本橋新一郎
  • Organizer: 第32 回日本リンパ学会総会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-06
• [Presentation] Phase I/II study of αGalcer-pulsed dendritic cell in patients with advanced or recurrent non-small cell lung cancer (2008)
  • Author(s): Shinichiro Motohashi
  • Organizer: 2008 American Society of Clinical Oncology Annual Meeting
  • Place of Presentation: Chicago, USA
  • Year and Date: 2008-05-31
• [Presentation] Phase I/II study of α GalCer-pulsed dendriticcell in patients with advanced or recurrentnon-small cell lung cancer (2008)
  • Author(s): Motohashi, S
  • Organizer: 2008 American Society of Clinical Oncologynnual Meeting
  • Place of Presentation: Chicago
  • Year and Date: 2008-05-29
• [Presentation] Natural Killer T cell-based Immunotherapyfor Lung Cancer (2008)
  • Author(s): 本橋新一郎
  • Organizer: 第12回基盤的癌免疫研究会
  • Place of Presentation: 大宮
  • Year and Date: 2008-01-03
• [Presentation] Phase I/II study of αGalCer-pulsed dendriticcell in patients with advanced or recurrentnon-small cell lung cancer (2007)
  • Author(s): 本橋新一郎
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-03
• [Presentation] 原発性肺癌に対するNKT細胞免疫系を標的にした免疫細胞療法 (2007)
  • Author(s): 本橋新一郎
  • Organizer: 第11回基盤的癌免疫研究会
  • Place of Presentation: 大宮
  • Year and Date: 2007-07-12
• [Presentation] 原発性肺癌術後再発抑制を目的としたα-GalactosylCeramide パルス樹状細胞投与によるNKT 細胞免疫療法 (2007)
  • Author(s): 本橋新一郎
  • Organizer: 第107回日本外科学会定期学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2007-04-12
• [Presentation] A phase l/ll study of αGalCer-pulsed dendritic cells in patients with a dvanced or recurrent non-small cell lung cancer (2007)
  • Author(s): Motohashi, S.
  • Organizer: 66th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Yokohama
• [Presentation] A phade l/ll study of αGalCer-pulsed dendritic cells for lung cancer (2007)
  • Author(s): Motohashi, S.
  • Organizer: 37th Anual Meeting of the Japanese Society for Immunology
  • Place of Presentation: Shinagawa
• [Remarks]
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• [Remarks]
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