Prediction of lymph node metastasis by gene expression profiling in patients with primary resected lung cancer

• Project/Area Number: 19591610
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Kitasato University (2008); Chiba University (2007)
• Principal Investigator: IYODA Akira Kitasato University, 医学部, 准教授 (10302548)
• Co-Investigator(Kenkyū-buntansha): SATOH Yukitoshi 北里大学, 医学部, 教授 (90321637) ; AMANO Hideki 北里大学, 医学部, 助教 (60296481) ; 関 直彦 千葉大学, 大学院・医学研究院, 准教授 (50345013) ; 渋谷 潔 千葉大学, 医学部附属病院, 講師 (20302565) ; 廣島 健三 千葉大学, 大学院・医学研究院, 准教授 (80218833)
• Co-Investigator(Renkei-kenkyūsha): SEKI Naohiko 千葉大学, 大学院・医学研究院, 准教授 (50345013) ; SHIBUYA Kiyoshi 千葉大学, 大学院・医学研究院, 准教授 (20302565) ; HIROSHIMA Kenzo 千葉大学, 大学院・医学研究院, 准教授 (80218833)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 呼吸器外科学 / 癌 / マイクロアレイ / 外科 / -マイクロアレイ

Research Abstract

100例の肺癌患者からの手術後摘出検体より腫瘍組織検体と正常肺組織検体を採取し、個々の検体を匿名化した状態で、oligoDNAマイクロアレイ解析を行った。対象としたDNAは既知の機能を有した10000遺伝子とし、その発現解析を行い、リンパ節転移予測遺伝子として15遺伝子、早期再発予測遺伝子として40遺伝子を抽出した。数遺伝子に関して、マイクロアレイ解析と定量PCRの結果が相関することを確認した。

Research Products

• [Journal Article] Prediction of lymph node metastasis by gene expression profiling in patients with primary resected lung cancer (2009)
  • Author(s): Moriya Y, Iyoda A, Kasai Y, Sugimoto T, Hashida J, Nimura Y, Kato M, Takiguchi M, Fujisawa T, Seki N, Yoshino I
  • Journal Title: Lung Cancer 64 Pages: 86-91
  • Peer Reviewed
• [Journal Article] Prediction of lymph node metastasis by gene expression profiling in patients with primary resected lung cancer (2009)
  • Author(s): Moriya Y, Iyoda A, et al.
  • Journal Title: Lung Cancer (In press) Pages: 86-91
  • Peer Reviewed
• [Journal Article] Postoperative recurrence and the role of adjuvant chemotherapy in patients with pulmonary large cell neuroendocrine carcinoma (2009)
  • Author(s): Iyoda A, et al.
  • Journal Title: J Thorac Cardiovasc Surg (In press)
  • Peer Reviewed
• [Journal Article] Predictors of postoperative survival in patients with locally advanced non-small cell lung carcinoma (2009)
  • Author(s): Iyoda A, et al.
  • Journal Title: Surg Today (In press)
  • NAID: 10027396937
  • Peer Reviewed
• [Journal Article] Diffuse malignant pleural mesothelioma : a multi-institutional clinicopathological study (2008)
  • Author(s): Iyoda A, Yusa T, Kadoyama C, Sasaki K, Kimura H, Yamakawa H, Shiba M, Fujisawa T, Yoshino I
  • Journal Title: Surg Today 38 Pages: 993-8
  • NAID: 10024429073
  • Peer Reviewed
• [Journal Article] Postoperative recurrence and the role of adjuvant chemotherapy in patients with pulmonary large cell neuroendocrine carcinoma
  • Author(s): Iyoda A, Hiroshima K, Moriya Y, Iwadate Y, Takiguchi Y, Uno T, Nakatani Y, Yoshino I
  • Journal Title: J Thorac Cardiovasc Surg (in press)
  • Peer Reviewed
• [Journal Article] Predictors of postoperative survival in patients with locally advanced non-small cell lung carcinoma
  • Author(s): Iyoda A, Hiroshima K, Moriya Y, Yoshida S, Suzuki M, Shibuya K, Yoshino I
  • Journal Title: Surg Today (in press)
  • NAID: 10027396937
  • Peer Reviewed
• [Presentation] 肺大細胞神経内分泌癌に対する治療戦略 (2008)
  • Author(s): 伊豫田明、原英則、蒋世旭, 小川史洋、松井啓夫、天野英樹、岡安勲吉村博邦、佐藤之俊
  • Organizer: 第49回日本肺癌学会総会ワークショップ
  • Place of Presentation: 北九州市
  • Year and Date: 2008-11-13
• [Presentation] 肺大細胞神経内分泌癌切除症例の検討 (2008)
  • Author(s): 伊豫田明、原英則、蒋世旭, 松井啓夫、小川史洋、天野英樹、岡安勲吉村博邦、佐藤之俊
  • Organizer: 第61回日本胸部外科学会定期学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2008-10-13