Tai-or-madetreat ment for lung cancer using EBUS-TBNA

• Project/Area Number: 19591611
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Chiba University
• Principal Investigator: SUZUKI Makoto (2008) Chiba University, 医学部・附属病院, 講師 (80312940); 安福 和弘 (2007) Chiba University, 医学部附属病院 (60372356)
• Co-Investigator(Kenkyū-buntansha): FUJIWARA Taiki 千葉大学, 医学部・附属病院, 助教 (30451743) ; SHIBUYA Kiyoshi 千葉大学, 医学部・附属病院, 講師 (20302565) ; SEKINE Yasuo 千葉大学, 大学院・医学研究院, 講師 (70312957) ; SUZUKI Makoto 千葉大学, 大学院・医学研究院, 助教 (80312940) ; YOSHIDA Shigetoshi 千葉大学, 医学部・附属病院, 助教 (90334200) ; NAKAJIMA Takahiro 千葉大学, 医学部・附属病院, 医員 (20400913) ; LYODA Akira 千葉大学, 医学部・附属病院, 助教 ; HIROSHIMA Kenzo 千葉大学, 大学院・医学研究院, 准教授 (80218833)
• Co-Investigator(Renkei-kenkyūsha): SHIBUYA Kiyoshi 千葉大学, 大学院・医学研究院, 准教授 (20302565) ; YOSHIDA Shigetoshi 千葉大学, 大学院・医学研究院, 助教 (90334200) ; HIROSHIMA Kenzo 千葉大学, 大学院・医学研究院, 准教授 (80218833) ; NAKAJIMA Takahiro 千葉県がんセンター, 呼吸器科, 医員 (20400913)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 肺癌 / 異常メチル化 / 遺伝子 / 外科 / 遺伝子発現解析 / 超音波気管支鏡ガイド下針生検 / 抗癌剤感受性

Research Abstract

EBUS-TBNAを1000例以上に施行し、それらの微量サンプルからDNA・RNAを抽出、基礎実験を行う系を確立した。突然変異解析、異常メチル化解析、RNA発現解析、蛋白発現解析が可能となった。肺癌に対するEBUS-TBNAを取り入れた、治療戦略を確立し、臨床試験を開始した。

Research Products

• [Journal Article] Nuclear survivin in pN2 nonsmall cell lung cancer : prognostic and clinical implications (2009)
  • Author(s): Mohamed, S., Yasufuku, K., Nakajima, T., Hiroshima, K., Chiyo, M., Yoshida, S., Suzuki, M., Sekine, Y., Shibuya, K., Agamy, G, El-Shahhat, H., Fujisawa, T., Yoshino, I.
  • Journal Title: Eur Respir J 33(1) Pages: 127-33
  • Peer Reviewed
• [Journal Article] Nuclesr survivin in pN2 nonsmall cell lung cancer:prognostic and clinical implications. (2009)
  • Author(s): Mohamed S, et al
  • Journal Title: Eur Respir J. 13 Pages: 127-133
  • Peer Reviewed
• [Journal Article] Identification of micro RNAs caused by DNA methylation that induce metastasis (2008)
  • Author(s): Suzuki M, Ichiro Yoshino
  • Journal Title: Future Oncol 4(6) Pages: 775-7
• [Journal Article] Clinical significance of aberrant methylation of prostaglandin E receptor 2 (PTGER2) in nonsmall cell lung cancer : association with prognosis, PTGER2 expression, and epidermal growth factor receptor mutation (2008)
  • Author(s): Tian, L., Suzuki, M., Nakajima, T., Kubo, R., Sekine, Y., Shibuya, K., Hiroshima, K., Nakatani, Y., Fujisawa, T., Yoshino, I
  • Journal Title: Cancer 113(6) Pages: 1396-403
  • Peer Reviewed
• [Journal Article] PIK3CA Mutations and Copy Number Gains in Human Lung Cancers (2008)
  • Author(s): Yamamoto, H., Shigematsu, H., Nomura, M., Lockwood, WW., Sato, M., Okumura, N., Soh, J., Suzuki, M., Wistuba, II., Fong, KM., Lee, H., Toyooka, S., Date, H., Lam, WL., Minna, JD., Gazdar, AF
  • Journal Title: Cancer Res 68 Pages: 6913-21
  • Peer Reviewed
• [Journal Article] Aberrant methylation of CXCL12 in non-small cell lung cancer is associated with unfavorable prognosis (2008)
  • Author(s): Suzuki, M., Mohamed, S., Nakajima, T., Kubo, R., Tian, L., Fujiwara, T., Suzuki, H., Nagato, K., Chiyo, M., Motohashi, S., Yasufuku, K., Iyoda, A., Yoshida, S., Sekine, Y., Shibuya, K., Hiroshima, K. Nakatani,Y., Yoshino,I., Fujisawa,T.
  • Journal Title: Int J Oncol 33(1) Pages: 113-9
  • Peer Reviewed
• [Journal Article] Role of endobronchial ultrasound-guided transbronchial needle aspiration in the management of lung cancer (2008)
  • Author(s): Yasufuku, K., Nakajima, T., Fujiwara, T., Chiyo, M., Iyoda, A., Yoshida, S., Suzuki, M., Sekine, Y., Shibuya, K., Yoshino, I.
  • Journal Title: Gen Thorac Cardiovasc Surg 56(6) Pages: 268-76
  • NAID: 10024308297
• [Journal Article] Analysis of cell cycle-related proteins in mediastinal lymph nodes of N2-NSCLC patients obtained by EBUS-TBNA : Relevance to chemotherapy response (2008)
  • Author(s): Mohamed, S., Yasufuku, K., Nakajima, T., Hiroshima, K., Kubo, R., Iyoda, A., Yoshida, S., Suzuki, M., Sekine, Y., Shibuya, K., Farouk, A., Fujisawa, T
  • Journal Title: Thorax 63(7) Pages: 642-7
  • Peer Reviewed
• [Journal Article] Clinical significance of aberrant methylation of prostaglandin E receptor 2 (PTGER2) in nonsmall cell ling cancer:association with prognosis, PTGER2 expression, and epidermal growth factor receptor mutation. (2008)
  • Author(s): Tian L, et al
  • Journal Title: Cancer 113 Pages: 1396-1403
  • Peer Reviewed
• [Journal Article] The evaluation of lymph node metastasis by EBUS-TBNA : crucial for selection of surgical candidates in metastatic lung tumors (2007)
  • Author(s): Nakajima, T., Yasufuku, K., Iyoda, A., Yoshida, S., Suzuki, M., Sekine, Y., Shibuya, K., Hiroshima, K., Nakatani, Y., Fujisawa, T.
  • Journal Title: J Thorac Cardiov Surg 134(6) Pages: 1485-90
  • Peer Reviewed
• [Journal Article] Synchronous alterations of Wnt and EGFR signaling pathways through aberrant methylation and mutation in non-small cell lung cancer (2007)
  • Author(s): Suzuki, M., Shigematsu, H., Nakajima, T., Kubo, R., Motohashi, S., Sekine, Y., Shibuya, K., Iizasa, T., Hiroshima, K., Nakatani, Y., Gazdar, AF., Fujisawa, T
  • Journal Title: Clin Cancer Res 15 ; 13(20) Pages: 6087-92
  • NAID: 130000123007
  • Peer Reviewed
• [Journal Article] Assessment of epidermal growth factor receptor mutation by endobronchial ultrasound-guided transbronchial needle aspiration (2007)
  • Author(s): Nakajima, T., Yasufuku, Y., Suzuki, M., Hiroshima, K., Kubo, R., Mohamed, S., Miyagi, Y., Matsukuma, S., Sekine, Y., Fujisawa, T.
  • Journal Title: Chest 132(2) Pages: 597-602
  • Peer Reviewed
• [Journal Article] Prognostic implications of cell cycle-related proteins in primary resectable pathologic N2 nonsmall cell lung cancer (2007)
  • Author(s): Mohamed, S., Yasufuku, K., Hiroshima, K., Nakajima, T., Yoshida, S., Suzuki, M., Sekine, Y., Shibuya, K., Iizasa, T., Farouk, A., Fujisawa, T.
  • Journal Title: Cancer 15 15 ; 109(12) Pages: 2506-14
  • Peer Reviewed
• [Journal Article] Prognostic imphcations of cell cycle-related proteins in primary resectable pathologic N2 nonsmall cell lung cancer (2007)
  • Author(s): Mohamed S, et. al.
  • Journal Title: Cancer 109 Pages: 2506-2514
  • Peer Reviewed
• [Journal Article] Endobronchial ultrasound guided transbronchial needle aspiration (EBUS-TBNA) for the evaluation of the mediastinum (2007)
  • Author(s): Yasufuku K, et. al.
  • Journal Title: Kyoubu Geka 60 Pages: 711-717
  • NAID: 40015568610
  • Peer Reviewed
• [Journal Article] Endobronchial ultrasonography: current status and fhture dhrections (2007)
  • Author(s): Yasufttku K, et. al.
  • Journal Title: Journal of Thoracic Oncology 2 Pages: 970-979
  • Peer Reviewed
• [Journal Article] Assessment of epidermal growth factor receptor mutation by endobronchial ultrasound-guided transbronchial needle aspiration (2007)
  • Author(s): Nakajima T, et. al.
  • Journal Title: Chest 132 Pages: 597-602
  • Peer Reviewed
• [Journal Article] The evaluation of lymph node metastasis by endobronchial ultrasound-guided transbronchial needle aspiration: crucial for selection of surgical candidates with metastatic lung tumors (2007)
  • Author(s): Nakajima T, et. al.
  • Journal Title: J Thoracic Cardiovascular Surgery 134 Pages: 1485-1490
  • Peer Reviewed
• [Journal Article] Aberrant methylation of CXCL12 in non-small cell lung cancer is associated with unfavorable prognosis.
  • Author(s): Suzuki M, et al
  • Journal Title: Int J Oncol
• [Presentation] COPD合併非小細胞肺癌における遺伝子異常メチル化プロフィールの検討 (2008)
  • Author(s): 鈴木実、和田啓伸、芳野充、田雷、重松久之、守屋康充、渋谷潔、吉田成利、田村創、藤原大樹、鈴木秀海、長門芳、星野英久、本橋新一郎、廣島健三、中谷行雄、吉野一郎
  • Organizer: 第48回日本肺癌学会総会
  • Place of Presentation: 北九州市
  • Year and Date: 2008-11-13
• [Presentation] 非小細胞肺癌におけるNMDAR2発現調節機構と臨床的意義 (2008)
  • Author(s): 鈴木実、芳野充、田雷、守屋康充、吉田成利、渋谷潔、星野英久、吉野一郎
  • Organizer: 第61日本胸部外科学会定期学術集会
  • Place of Presentation: 福岡市
  • Year and Date: 2008-10-13
• [Presentation] 非小細胞肺癌におけるNMDAR2発現調節機構と臨床的意義 (2008)
  • Author(s): 鈴木実, 他
  • Organizer: 第61回日本胸部外科学会定期学術集会
  • Place of Presentation: 福岡市
  • Year and Date: 2008-10-13
• [Presentation] 非小細胞肺癌におけるProstaglandin E Receptor 2の異常メチル化とEGFR変異の関係について (2008)
  • Author(s): 田雷、鈴木実、中島崇裕、窪理英子、安福和弘、伊豫田明、吉田成利、関根康雄、渋谷潔、廣島健三、中谷行雄、藤澤武彦、吉野一郎
  • Organizer: 第25回日本呼吸器外科学会総会
  • Place of Presentation: 宇都宮市
  • Year and Date: 2008-05-30
• [Presentation] 非小細胞肺癌におけるNMDAR2Bの発現に関する検討 (2008)
  • Author(s): 鈴木実、中島崇裕、窪理英子、田雷、安福和弘、伊豫田明、吉田成利、関根康雄、渋谷潔、藤澤武彦、吉野一郎
  • Organizer: 第25回日本呼吸器外科学会総会
  • Place of Presentation: 宇都宮市
  • Year and Date: 2008-05-29
• [Presentation] 非小細胞肺癌におけるProstaglandin E Receptor 2の異常メチル化とFGFR変異の関係について (2008)
  • Author(s): 田雷, 他
  • Organizer: 第25回日本呼吸器外科学会総会
  • Place of Presentation: 宇都宮市
  • Year and Date: 2008-05-29
• [Presentation] 原発性非小細胞肺癌におけるWntシグナルとEGFRシグナルの同期的異常 (2007)
  • Author(s): 鈴木実, 重松久之, 中島崇裕, 窪理恵子, 本橋新一郎, 関根康雄, 渋谷潔, 飯笹俊彦, 廣島健三, 中谷行雄, 吉野一郎, 藤沢武彦
  • Organizer: 第47回日本肺癌学会総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2007-11-08
• [Presentation] 肺癌におけるDNAミスマッチ修復遺伝子発現低下がp53・EGFR遺伝子変異に及ぼす影響について (2007)
  • Author(s): 鈴木実, 大平美紀, 芳野充, 重松久之, 影山肇, 飯笹俊彦, 中川原章, 木村秀樹
  • Organizer: 第47回日本肺癌学会総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2007-11-08
• [Presentation] EBUS-TBNA検体を用いたDNA異常メチル化解析と抗癌剤感受性予測 (2007)
  • Author(s): 中島 崇裕, ら
  • Organizer: 第48回日本肺癌学会総会
  • Place of Presentation: 愛知県 名古屋市
  • Year and Date: 2007-11-08
• [Presentation] 肺癌術前・術後治療戦略におけるEBUS-TBNAの役割-肺門・縦隔リンパ節転移診断の意義 (2007)
  • Author(s): 安福 和弘, ら
  • Organizer: 第48回日本肺癌学会総会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2007-11-08
• [Presentation] Utility of endobronchial ultrasound-guided transbronchial needle aspiration in the diagnosis of mediastinal masses of unknown origin (2007)
  • Author(s): Yasufuku, et. al.
  • Organizer: American College of Chest Physicians 2007 Annual Meeting
  • Place of Presentation: Chicago,USA
  • Year and Date: 2007-10-23
• [Presentation] Prognostic implication of cell cycle-related proteins in primary resectable pn2 non-small cell lung cancer (2007)
  • Author(s): Yasufuku, et. al.
  • Organizer: 12thWorld Conference on Lung Cancer
  • Place of Presentation: Seoul,Korea
  • Year and Date: 2007-09-06
• [Presentation] Lymph node mapping by EBUS-TBNA.Interactive Grand Rounds (2007)
  • Author(s): Yasufuku, et. al.
  • Organizer: 12thWorld Conference on Lung Cancer
  • Place of Presentation: Seoul,Korea
  • Year and Date: 2007-09-03
• [Presentation] Evaluating the Mediastinum:Bronchoscopic Techniques (2007)
  • Author(s): Yasufuku, et. al.
  • Organizer: 2ndAsian Pacdic Congress of Brollchology
  • Place of Presentation: Singapore
  • Year and Date: 2007-07-13
• [Presentation] A prospective controlled trial of EBUS-TBNA compared to mediastinoscopy for mediastinal lymph node staging of lung cancer (2007)
  • Author(s): Yasufuku, et. al.
  • Organizer: 33rdWestern Thoracic Surgery Association Annual Meeting
  • Place of Presentation: New Mexico,USA
  • Year and Date: 2007-06-28
• [Presentation] 肺癌におけるEBUS-TBNAによる系統的肺門・縦隔リンパ節サンプリング (2007)
  • Author(s): 安福 和弘, ら
  • Organizer: 第32回日本外科系連合学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2007-06-22
• [Presentation] EBUS-TBNAによる肺癌リンパ節転移診断-細胞診から分子生物学的解析へ (2007)
  • Author(s): 安福 和弘, ら
  • Organizer: 第48回日本臨床細胞学会総会
  • Place of Presentation: 千葉県千葉市
  • Year and Date: 2007-06-08
• [Presentation] Bronchosocpy Chnic:State of the Art-Bronchoscopic Ultrasound (2007)
  • Author(s): Yasufuku, et. al.
  • Organizer: 2007 American Thoracic Society, InternationaConference
  • Place of Presentation: San Francisco,USA
  • Year and Date: 2007-05-24
• [Presentation] EBUS-TBNA in patients with previous malignancy and mediastinal and/or hiar lymphadenopathy (2007)
  • Author(s): Yasufuku, et. al.
  • Organizer: 2007 American Thoracic Society, International Conference
  • Place of Presentation: San Francisco,USA
  • Year and Date: 2007-05-23
• [Presentation] Endobronchial Ultrasound-EBUS in Early Stage Cancer (2007)
  • Author(s): Yasufuku, et. al.
  • Organizer: 2007 American Thoracic Society, International Conference
  • Place of Presentation: San Francisco,USA
  • Year and Date: 2007-05-22
• [Presentation] Epidermal growth factor receptor analysis by endobronchial ultrasound guided transbronchial needle aspiration (2007)
  • Author(s): Nakajima, et. al.
  • Organizer: 2007 American Thoracic Society, International Conference
  • Place of Presentation: San Francisco,USA
  • Year and Date: 2007-05-22
• [Presentation] 肺癌におけるIL-12RP2異常メチル化の検討 (2007)
  • Author(s): 鈴木実, 飯笹俊彦, 中島崇裕, 窪理恵子 ,伊豫田明, 廣島健三, 中谷行雄, 藤澤武彦
  • Organizer: 第24回日本呼吸器外科学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-05-17
• [Book] 目で見てわかる、超音波気管支鏡ガイド下針生検マニュアル (2007)
  • Author(s): 安福 和弘, ら
  • Total Pages: 89
  • Publisher: 金原出版株式会社
• [Remarks]
  • URL: http://www.ho.chiba-u.ac.jp/18/
• [Remarks]
  • URL: http://www.ho.chiba-u,ac,jp/18/