A novel drug delivery system via inhalation of nanoparticles

• Project/Area Number: 19591623
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Kyoto University
• Principal Investigator: FUJINAGA Takuji Kyoto University, 医学研究科, 助教 (00444456)
• Co-Investigator(Kenkyū-buntansha): BANDO Toru 京都大学, 医学研究科, 准教授 (20293954) ; CHIN Toyofumi 京都大学, 医学研究科, 助教 (00452334) ; 秋吉 一成 東京医科歯科大学, 生体材料工学研究所, 教授 (90201285)
• Co-Investigator(Renkei-kenkyūsha): AKIYOSHI Kazunari 東京医科歯科大学, 生体材料工学研究所, 教授 (90201285)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ナノ粒子 / DDS / 肺移植 / 心臓死ドナー / 心停止ドナー / 吸入

Research Abstract

ドナー肺の有効利用を可能にするため、吸入投与が不可能と思われる薬剤の新しいDrugdelivery systemの開発研究を行った。コレステロールプルランCHPを蛍光標識し、ラットに経静脈投与と吸入による投与を行ったところ、加アミノ基CHP(CHP-NH2)の吸入が肺組織へもっとも取り込まれた。さらに、ヒト心房利尿ペプチドをCHP-NH2に包埋させて吸入を行ったところ、薬剤単独吸入に比べ、肺血管拡張、肺組織中cGMPといった局所作用のみならず、全身血流にも薬剤が分布する可能性が示唆された。

Research Products

• [Journal Article] Protective effect of thioredoxin perfusion but not inhalation in warm ischemic-reperfused rat lungs (2009)
  • Author(s): Zhang J, Chen F, Nakamura T, Fujinaga T, Aoyama A, et al.
  • Journal Title: Redox Report
  • Peer Reviewed
• [Journal Article] Nebulized phosphodiesterase III inhibitor during warm ischemia attenuates pulmonary ischemia-reperfusion injury (2009)
  • Author(s): Zhang J, Chen F, Zhao X, Aoyama A, Okamoto T, Fujinaga T, et al.
  • Journal Title: J Heart Lung Transplant 28(1) Pages: 79-84
  • Peer Reviewed
• [Journal Article] POSTISCHEMIC INFUSION OF ATRIAL NATRIURETIC PEPTIDE ATTENUATES WARM ISCHEMIA-REPERFUSION INJURY IN RAT LUNG (2009)
  • Author(s): Aoyatma A, Chen F, Fujinaga T, Sato A, Tsuruyama T, et al.
  • Journal Title: J Heart Lung Transplant
  • Peer Reviewed
• [Journal Article] Potential for Pulmonary Protection by Nebulized Milrinone during Warm Ischemia (2008)
  • Author(s): Chen F, Zhang J, Aoyama A, Okamoto T, Fujinaga T, Bando T.
  • Journal Title: Transplant Proc 40(10) Pages: 335-8
  • Peer Reviewed
• [Journal Article] Expression of endothelial specific adhesion molecules after cardiac arrest (2008)
  • Author(s): Chen F, Kondo N, Sonobe M, Fujinaga T, Wada H, Bando T.
  • Journal Title: Interact Cardiovasc Thorac Surg 7(3) Pages: 437-40
  • Peer Reviewed
• [Journal Article] Postischemic Infusion of Atrial Natriuretic Peptide Attenuates Warm Ischemia-Reperfusion Injury in Rat Lung
  • Author(s): Aoyama A, Chen F, Fujinaga T, Okamoto T, Sato A, Tsuruyama T, Zhang J, Shoji T, Sakai H, Nakamura T, Date H, Wada H, Bando T.
  • Journal Title: J Heart Lung Transplant (in press)
  • Peer Reviewed
• [Journal Article] Protective effect of thioredoxin perfusion but not inhalation in warm ischemic-reperfused rat lungs
  • Author(s): Zhang J, Chen F, Nakamura T, Fujinaga T, Aoyama A, Hamakawa H, Sakai H, Hoshino Y, Yodoi J, Wada H, Bando T, Nakamura H.
  • Journal Title: Redox Report accepted
  • Peer Reviewed
• [Journal Article] Expression of endothelial specific adhesion molecules after cardiac arrest
  • Author(s): Chen F, Kondo N, Sonobe M, Fujinaga T, Wada H, Bando T
  • Journal Title: Int Cardiovasc Thorac Surg (In press)
  • Peer Reviewed
• [Journal Article] Potential for Pulmonary Protection by Nebulized Milrinone during Warm Ischemia
  • Author(s): Chen F, Zhang J, Aoyama A, Okamoto T, Fujinaga T, and Bando
  • Journal Title: Transplant Proc (In press)
  • Peer Reviewed
• [Presentation] わが国のDCDからの肺移植実験に向けての実験的取り組み (2008)
  • Author(s): 岡本俊宏、陳豊史、藤永卓司、張吉天、青山晃博、趙向東、高橋鮎子、里田直樹、山田徹、森川洋匡、庄司剛、阪井宏彰、松本いずみ、板東徹、伊達洋至
  • Organizer: 第44回日本移植学会総会
  • Place of Presentation: 大阪市
  • Year and Date: 2008-09-21
• [Presentation] 心臓死ドナーからの肺移植に向けた、ドナー肺の評価とその保護の研究 (2008)
  • Author(s): 板東徹、陳豊史、藤永卓司、張吉天、青山晃博、岡本俊宏、趙向東、高橋鮎子、里田直樹、庄司剛、阪井宏彰
  • Organizer: 第44回日本移植学会総会
  • Place of Presentation: 大阪市
  • Year and Date: 2008-09-21
• [Presentation] 心臓死ドナーからの肺移植に向けた、ドナー肺の評価とその保護の研究 (2008)
  • Author(s): 陳豊史、藤永卓司、張吉天、青山晃博、岡本俊宏、趙向東、高橋鮎子、里田直樹、庄司剛、阪井宏彰、板東徹
  • Organizer: 第108回日本外科学会定期学術集会
  • Place of Presentation: 長崎市(WS7-2)
  • Year and Date: 2008-05-16
• [Presentation] 心臓死ドナーからの肺移植に向けた、ドナー肺の評価とその保護の研究 (2008)
  • Author(s): 陳豊史, 藤永卓司, 張吉天, 青山晃博, 岡本俊宏, ほか。
  • Organizer: 第108回日本外科学会定期学術集会
  • Place of Presentation: 長崎市
  • Year and Date: 2008-05-16
• [Presentation] Nanoparticle of cholesterol-bearing pullulan as a carrier of anticancer drug (2008)
  • Author(s): Sato K, Chen F, Aoyama A, Akiyoshi K, Date H.
  • Organizer: 20th Meeting of the European Association for Cancer Research
  • Place of Presentation: Lyon, France
• [Presentation] 肺移植ドナープール拡大の試み (2007)
  • Author(s): 板東徹、陳豊史、藤永卓司、張吉天、趙向東、高橋鮎子、岡本俊宏、青山晃博、里田直樹、浜川博司、庄司剛、阪井宏彰、松本いずみ、和田洋巳
  • Organizer: 第34回日本臓器保存生物医学会総会
  • Place of Presentation: 札幌市
  • Year and Date: 2007-11-16
• [Presentation] Human atrial natriuretic peptide ameliorates warm ischemia-reperfusion injury of the lung. Abstract, Abstract # (2007)
  • Author(s): Aoyama A, Chen F, Nakamura T, Fujinaga T, Zhang JT, Okamoto T, Wada H, Bando T.
  • Organizer: 27th Annual Meeting of the International Society for Heart and Lung Transplantation
  • Place of Presentation: San Francisco
• [Remarks]
  • URL: http://www.thoracic-kyoto-u.gr.jp/