The role and relative mechanisms of hemodynamic loading in myocardial repair

• Project/Area Number: 19591628
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Yamaguchi University
• Principal Investigator: MIKAMO Akihito Yamaguchi University, 大学院・医学系研究科, 講師 (30372709)
• Co-Investigator(Kenkyū-buntansha): LI Taosheng 山口大学, 大学院・医学系研究科, 助教 (50379997)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: メカニカルストレス / 心筋再生 / 細胞増殖 / アポトーシス / 心筋幹細胞

Research Abstract

心臓がポンプ臓器であるが故に常に被るメカニカルストレスが心筋再生に悪影響を及ぼすと仮説を立て、検証を行った。マウス心筋梗塞作製後に梗塞心を摘出し、別マウスの腹腔内に異所性心移植を行い、メカニカルストレス軽減モデルを作製した。対照群と比較して、壁厚が保たれ、梗塞面積も縮小していた。また、細胞増殖の増加やアポトーシスの減少、障害心筋内への幹細胞集積が認められた。以上から、メカニカルストレス軽減が心筋再生を促すことが示された。

Research Products

• [Journal Article] Ischemic pre-conditioning enhances the mobilization and recruitment of bone marrow stem cells to protect against ischemia/ reperfusion injury in the late phase (2009)
  • Author(s): Kamota T, Li TS, Morikage N, Murakami M, Ohshima M, Kubo M, Kobayashi T, Mikamo A, Ikeda Y, Matsuzaki M, Hamano K
  • Journal Title: J Am Coll Cardiol 53(19) Pages: 1814-22
  • Peer Reviewed
• [Journal Article] Ischemic preconditioning enhances the mobilization and recruitment of bonemarrow stem cells to protect against ischemia/reperfusion injury in the late phase (2009)
  • Author(s): Kamota T, et al
  • Journal Title: Journal of the American College of Cardiology (in press)
  • Peer Reviewed
• [Journal Article] Myocardial repair achieved by the intramyocardial implantation of adult cardiomyocytes in combination with bone marrow cells (2008)
  • Author(s): Li TS, Takahashi M, Ohshima M, Qin SL, Kubo M, Muramatsu K, Hamano K
  • Journal Title: Cell Transplant 17(6) Pages: 695-703
  • Peer Reviewed
• [Journal Article] Myocardial repair achieved by the intramyocardial implantation of adult cardiomyocytes in combination with bone marrow cells (2008)
  • Author(s): Li TS, et al
  • Journal Title: Cell Transplantation 17 Pages: 695-703
  • Peer Reviewed
• [Journal Article] The reduction of hemodynamic loading assists self-regeneration of the injured heart by increasing cell proliferation inhibiting cell apoptosis, and inducing stem-cell recruitment (2007)
  • Author(s): Suzuki R, Li TS, Mikamo A, Takahashi M, Ohshima M, Kubo M, Ito H, Hamano K.
  • Journal Title: J Thorac Cardiovasc Surg 133(4) Pages: 1051-8
  • Peer Reviewed
• [Journal Article] Long-term efficacy and safety of the intramyocardial implantation of autologous bone marrow cells for the treatment of ischemic heart disease (2007)
  • Author(s): Li TS, Murakami M, Kobayashi T, Shirasawa B, Mikamo A, Hamano K
  • Journal Title: J Thorac Cardiovasc Surg 134(5) Pages: 1347-9
  • Peer Reviewed
• [Journal Article] Comparison of cell therapy and cytokine therapy for functional repair in ischemic and nonischemic heart failure (2007)
  • Author(s): Li TS, Mikamo A, Takahashi M, Suzuki R, Ueda K, Ikeda Y, Matsuzaki M, Hamano K
  • Journal Title: Cell Transplant 16(4) Pages: 365-74
  • Peer Reviewed
• [Journal Article] Analysis of the origin and population dynamics of cardiac progenitor cells in a donor heart model (2007)
  • Author(s): Li TS, Suzuki R, Ueda K, Murata T, Hamano K
  • Journal Title: Stem Cells 25(4) Pages: 911-7
  • Peer Reviewed
• [Journal Article] The reduction of hemodynamic loading assists self-regeneration of the injured heart by increasing cell proliferation,inhibiting cell apoptosis,and inducing stem-cell recruitment (2007)
  • Author(s): Suzuki R, et. al.
  • Journal Title: J Thorac Cardiovasc Surg 133(4) Pages: 1051-1058
  • Peer Reviewed
• [Journal Article] Analysis of the origin and pouplation dynamics of cardiac progenitor cells in a donor heart model (2007)
  • Author(s): Li TS, et. al.
  • Journal Title: Stem cells 25(4) Pages: 911-917
  • Peer Reviewed
• [Presentation] Ischemic preconditioning enhances the mobilization and recruitment of bone marrow stem cells to protect against ischemia/ reperfusion injury in the late phase (2008)
  • Author(s): Kamota T, Li TS, Morikage N, Murakami M, Ohshima M, Kubo M, Kobayashi T, Mikamo A, Hamano K
  • Organizer: American Heart Association Scientific Sessions 2008
  • Place of Presentation: New Orleans(U.S.A.)
  • Year and Date: 2008-11-10
• [Presentation] Ischemic preconditioning enhances the mobilization and recruitment of bone marrowstem cells to protect against ischemia/reperfusion injury in the late phase (2008)
  • Author(s): Takahiro Kamota
  • Organizer: American Heart Association Scientific Sessions 2008
  • Place of Presentation: New Orleans (U.S.A.)
  • Year and Date: 2008-11-10
• [Presentation] 自己骨髄細胞を用いた血管再生療法の治療効果に及ぼす因子の探索 (2008)
  • Author(s): 李桃生、鴨田隆弘、佐藤正史、村上雅憲、小林俊郎、白澤文吾、美甘章仁、濱野公一
  • Organizer: 第38回日本心臓血管外科学会学術総会
  • Place of Presentation: 福岡
  • Year and Date: 2008-02-20
• [Presentation] 障害心筋における圧容量負荷の軽減は心筋再生を促す (2007)
  • Author(s): 鈴木亮、李桃生、村上雅憲、小林俊郎、白澤文吾、美甘章仁、濱野公一
  • Organizer: 第91回日本循環器学会中国地方会
  • Place of Presentation: 米子
  • Year and Date: 2007-12-01
• [Presentation] 心不全に対する自己骨髄細胞を用いた再生医療 : 臨床長期成績と心筋再生への展望 (2007)
  • Author(s): 李桃生、鈴木亮、村上雅憲、小林俊郎、白澤文吾、伊東博史、美甘章仁、濱野公一
  • Organizer: 第107回日本外科学会定期学術集
  • Place of Presentation: 大阪
  • Year and Date: 2007-04-11