Functional analysis of schizophrenia-susceptible gene dysbindin

• Project/Area Number: 19591661
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Chiba University
• Principal Investigator: YAMAGUCHI Atsushi Chiba University, 大学院・医学研究院, 准教授 (00314336)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 機能脳神経外科学 / 脳・神経 / 神経科学 / 統合失調症 / Sortin Nexin / dysbindin

Research Abstract

当該研究は統合失調症脆弱性遺伝子Dysbindinの神経細胞における機能解析を施行。Yeast Two-hybrid Systemによるスクリーニングで、結合タンパク質として膜タンパク質の輸送・sortingに関与するSNX5を同定した。細胞性免疫染色法により、DysbindinとSNX5は細胞質で共局在し、in situ hybridization法を用いた解析では、両者とも脳の広範囲で発現を認めた。Endocytosis解析で、両者の相互作用が受容体の取込み・分解に関与することが示唆された。

Research Products

• [Journal Article] Zyxin is a novel interacting partner for SIRT1 (2009)
  • Author(s): Fujita, Y., Yamaguchi, A. Hata, K., Endo, M., Yamaguchi, N. and Yamashita, T.
  • Journal Title: BMC Cell Biol (In press)
  • Peer Reviewed
• [Journal Article] Zyxin is a novel interacting partner for SIRT1 (2009)
  • Author(s): Fujita, Y., Yamaguchi, A. Hata, K., Endo, M., Yamaguchi, N. and Yamashita, T.
  • Journal Title: BMC Cell Biology (in press)
  • Peer Reviewed
• [Journal Article] Inhibition of eIF2B∂/F11A3.2 during adulthood extends lifespan in Caenorhabditis elegans (2008)
  • Author(s): Tohyama, D., Yamaguchi, A., and Yamashita, T.
  • Journal Title: FASEB J. 22 Pages: 4327-4337
  • Peer Reviewed
• [Journal Article] Peptides derived from repulsive guidance molecule act as antagonists (2008)
  • Author(s): Suda, M., Hata, K., Sawada, A., Nakamura,Y., Kubo., Yamaguchi, A. and Yamashita, T.
  • Journal Title: Biochem Biophys Res Commun 371 Pages: 501-504
  • Peer Reviewed
• [Journal Article] Peptides derived from repulsive guidance molecule act as antagonists (2008)
  • Author(s): Suda, M., Hata, K., Sawada, A., Nakamura, Y., Kubo., Yamaguchi, A. and Yamashita, T.
  • Journal Title: Biochem Biophys Res Commun 371 Pages: 501-504
  • Peer Reviewed
• [Journal Article] Inhibition of eIF2B∂/F11A3.2 during adulthood extends lifespan in Caenorhabditis elegans. (2008)
  • Author(s): Tohyama, D., Yamaguchi, A., and Yamashita. T.
  • Journal Title: FASEB J. 22 Pages: 4327-4337
  • Peer Reviewed
• [Journal Article] A novel DISCl-interacting partner DISCl-Binding Zinc-finger protein: implication in the modulation of DISCl-dependent neurite outgrowth (2007)
  • Author(s): Hattori T, Baba K, Matsuzaki S, Honda A. Miyoshi K, Inoue K, Taniguchi M, Hashimoto H, Shintani N, Baba A, Shimizu S. Yukioka F, Kumamoto N, Yamaguchi A, Tohyama M, Katayama T.
  • Journal Title: Mol Psychiatry 12(4) Pages: 398-407
  • Peer Reviewed
• [Journal Article] BMP inhibits neurite growth by a mechanism dependent on LIM-kinase (2007)
  • Author(s): Matsuura, I., Endo, M., Hata, K, Kubo, T, Yamaguchi, A. Saeki, N. and Yamashita, T
  • Journal Title: Biochem. Biophys. Res, Commun 360 Pages: 868-873
  • Peer Reviewed
• [Journal Article] Repulsion of cerebellar granule neurons by chondroitin sulfate proteoglycans is mediated by MAPK pathway (2007)
  • Author(s): Kaneko, M., Kubo, T, Hata, K., Yamaguchi, A. and Yamashita, T
  • Journal Title: Neurosci. Lett 423 Pages: 62-67
  • Peer Reviewed
• [Journal Article] Myosin IIA is required for outgrowth inhibition produced by repulsive guidance molecule.
  • Author(s): Kubo T, Endo M, Hata K, Taniguchi J, Kitajo K, Tomura S, Yamaguchi A, Mueller BK, Yamashita T.
  • Journal Title: J Neurochem. In perss
  • Peer Reviewed
• [Presentation] Inhibition of eIF2Bδ during adulthood extends lifespan in C.elegans (2008)
  • Author(s): 遠山大介, 山口淳, 山下俊英
  • Organizer: American Society of Cell Biology, 48th Annual Meeting
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2008-12-16
• [Presentation] Inhibition of eIF2Bδ during adulthood extends lifespan in C. elegans (2008)
  • Author(s): 遠山大介 山口淳 山下俊英
  • Organizer: American Society of Cell Biology, 48th Annual Meeting
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 2008-12-16
• [Presentation] Inhibition of eIF2Bδ during adulthood extends lifespan in C.elegans (2008)
  • Author(s): 遠山大介, 山口淳, 山下俊英
  • Organizer: BMB 2008 第31回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピアアイランド
  • Year and Date: 2008-12-10
• [Presentation] Inhibition of eIF2Bδ during adulthood extends lifespan in C. elegans (2008)
  • Author(s): 遠山大介 山口淳 山下俊英
  • Organizer: BMB 2008 第31回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピアアイランド
  • Year and Date: 2008-12-10
• [Presentation] 統合失調症脆弱性遺伝子dysbindinの機能解析 (2008)
  • Author(s): 遠山大介, 山口淳, 山下俊英
  • Organizer: 第29回日本臨床薬理学会年会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 2008-12-03
• [Presentation] 統合失調症脆弱性遺伝子dysbindinの機能解析 (2008)
  • Author(s): 遠山大介 山口淳 山下俊英
  • Organizer: 第29回日本臨床薬理学会年会
  • Place of Presentation: 新宿京王プラザホテル
  • Year and Date: 2008-12-03
• [Presentation] 老化の分子生物学的メカニズムの解明に向けて (2008)
  • Author(s): 山口 淳
  • Organizer: Chiba Basic and Clinical Conference
  • Place of Presentation: 千葉 京成ミラマーレ
  • Year and Date: 2008-02-09
• [Presentation] Identification of binding partners for the NAD-dependent deacetylase SIRT1 (2007)
  • Author(s): 藤田 幸, 山口 淳, 羽田 克彦, 遠藤 光晴, 山口 直人. 山下 俊英
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] RNAi screening for genes involved in dietary restriction in Celegans (2007)
  • Author(s): 遠山 大介 山口 淳 山下 俊英
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] NAD依存性ピストン脱アセチル化酵素SIRT1の結合因子の解析 (2007)
  • Author(s): 藤田 幸, 山口 淳, 羽田 克彦, 遠藤 光晴, 山口 直人, 山下 俊英
  • Organizer: 第51回 日本薬学会 関東支部大会
  • Place of Presentation: 星薬科大学
  • Year and Date: 2007-10-06
• [Presentation] Rapl is involved in the signal transduction of myelin-associated glycoprotein (2007)
  • Author(s): Junko Taniguchi, Masashi Fujitani, Mitsu haru Endo, Takekazu Kubo, Mayumi Fujitani, Katsuhiko Hata, Atsushi Yamacuchi, Freda D Miller, David R Kaplan, Toshi hide Yamashita
  • Organizer: Neuro 2007(Meeting of the Japan Neuroscience Society)
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-09-12
• [Presentation] 老化のメカニズムの解明に向けて (2007)
  • Author(s): 山口 淳
  • Organizer: 第9回ORIGIN神経科学研究会夏のワークショップ
  • Place of Presentation: 浜松舘山寺サゴーロイヤルホテル
  • Year and Date: 2007-09-01