The development of stereotactic deep brain stimulation and cell therapy for limbic seizure in rat

• Project/Area Number: 19591671
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Nagoya University
• Principal Investigator: KAJITA Yasukazu Nagoya University, 医学系・研究科, 准教授 (70303617)
• Co-Investigator(Kenkyū-buntansha): NATSUME Atsushi 名古屋大学, 医学部附属病院, 特任准教授 (30362255)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: てんかん / 脳深部刺激療法 / 細胞療法 / 視床下核学 / 視床下核 / 視床前核 / 骨髄間質細胞 / 海馬 / カイニン酸

Research Abstract

カイニン酸を、右海馬に定位的に注入し、辺縁系てんかんモデルを作成した。コントロール群は、両側深部電極と両側前頭部電極脳波記録にて、深部電極のみで発作波を記録する部分発作と、両側前頭部の硬膜外電極に発作波が伝わる全般化した発作が記録された。視床下核を深部刺激すると、発作の2次性全般化を抑制した。骨髄間質細胞を海馬に移植後、視床下核を深部刺激したが、部分発作には、有意な変化はなかった。

Research Products

• [Journal Article] p16 promoter methylation in the serum as a basis for the molecular diagnosis of gliomas (2009)
  • Author(s): Wakabayashi T, Natsume A, Hatano H, Fujii M, Shimato S, Ito M, Ohno M, Ito S, Ogura M, Yoshida J
  • Journal Title: Neurosurgery 64 Pages: 455-61
  • Peer Reviewed
• [Journal Article] Synergistic induction of NY-ESO-1 antigen expression by a novel histone deacetylase inhibitor, valproic acid, with 5-aza-2'-deoxycytidine in glioma cells (2009)
  • Author(s): Oi S, Natsume A, Ito M, Kondo Y, Shimato S, Maeda Y, Saito K, Wakabayashi T
  • Journal Title: J Neurooncol 92 Pages: 15-22
  • Peer Reviewed
• [Journal Article] Interferon-beta, MCNU, and conventional radiotherapy for pediatric patients with brainstem glioma (2009)
  • Author(s): Ohno M, Natsume A, Fujii M, Ito M, Wakabayashi T
  • Journal Title: Pediatr Blood Cancer
  • Peer Reviewed
• [Journal Article] Type I Interferon Inhibits Astrocytic Gliosis and Promotes Functional Recovery after Spinal Cord Injury by Deactivation of the MEK/ERK Pathway (2009)
  • Author(s): Ito M, Natsume A, Takeuchi H, Shimato S, Ohno M, Wakabayashi T, Yoshida J
  • Journal Title: J Neurotrauma 26 Pages: 41-53
  • Peer Reviewed
• [Journal Article] Type I Interferon Inhibits Astrocytic Gliosis and Promotes Functional Recovery after Spinal Cord Injury by Deactivation of the MEK/ERK Pathway. (2009)
  • Author(s): Ito M, Natsume A, Takeuchi H, et al.
  • Journal Title: Journal of Neurotrauma 26 Pages: 41-53
  • Peer Reviewed
• [Journal Article] てんかんの手術適応 (2008)
  • Author(s): 梶田泰一
  • Journal Title: 現代医学 58 Pages: 59-67
• [Journal Article] A phase I clinical trial of interferon-beta gene therapy for high-grade glioma : novel findings from gene expression profiling and autopsy (2008)
  • Author(s): Wakabayashi T, Natsume A, Hashizume Y, Fujii M, Mizuno M, Yoshida J
  • Journal Title: J Gene Med 10 Pages: 329-39
  • Peer Reviewed
• [Journal Article] Identification of a human leukocyte antigen-A24-restricted T-cell epitope derived from interleukin-13 receptor alpha2 chain, a glioma-associated antigen (2008)
  • Author(s): Shimato S, Natsume A, Wakabayashi T, Tsujimura K, Nakahara N, Ishii J, Ito M, Akatsuka Y, Kuzushima K, Yoshida J
  • Journal Title: Jpn J Clin Oncol 38 Pages: 715-8
  • Peer Reviewed
• [Journal Article] Identification of a human leukocyte antigen-A24-restricted T-cell epitope derived from interleukin-13 receptor alpha2 chain, a glioma-associated antigen (2008)
  • Author(s): Shimato S, Natsume A, Wakabayashi T, Tsujimura K, Nakahara N, Ishii J, Ito M, Akatsuka Y, Kuzushima K, Yoshida J
  • Journal Title: J Neurosurg 109 Pages: 117-22
  • Peer Reviewed
• [Journal Article] Gene therapy for high-grade glioma : current approaches and future directions (2008)
  • Author(s): Natsume A, Yoshida J
  • Journal Title: Cell Adh Migr 2 Pages: 186-91
  • Peer Reviewed
• [Journal Article] The DNA demethylating agent 5-aza-2'-deoxycytidine activates NY-ESO-1 antigenicity in orthotopic human glioma (2008)
  • Author(s): Natsume A, Wakabayashi T, Tsujimura K, Shimato S, Ito M, Kuzushima K, Kondo Y, Sekido Y, Kawatsura H, Narita Y, Yoshida J
  • Journal Title: Int J Cancer 122 Pages: 2542-53
  • Peer Reviewed
• [Journal Article] A combination of IFN-beta and temozolomide in human glioma xenograft models : implication of p53-mediated MGMT downregulation (2008)
  • Author(s): Natsume A, Wakabayashi T, Ishii D, Maruta H, Fujii M, Shimato S, Ito M, Yoshida J
  • Journal Title: Cancer Chemother Pharmacol 61 Pages: 653-9
  • Peer Reviewed
• [Journal Article] Variable DNA methylation patterns associated with progression of disease in hepatocellular carcinomas (2008)
  • Author(s): Gao W, Kondo Y, Shen L, Shimizu Y, Sano T, Yamao K, Natsume A, Goto Y, Ito M, Murakami H, Osada H, Zhang J, et.al.
  • Journal Title: Carcinogenesis 29 Pages: 1901-10
  • Peer Reviewed
• [Journal Article] The DNA demethylating agent 5-aza-2'-deoxycytidine activates NY-ESO-1 antigenicity in orthotopic human glioma. (2008)
  • Author(s): Natsume A, Wakabayashi T, Tsujimura K, et al.
  • Journal Title: International Journal of Cancer 122 Pages: 2542-2553
  • Peer Reviewed
• [Journal Article] Inhibition of Aurora-B function increases formation of multinucleated cells in p53 gene deficient cells and enhances anti-tumor effect of temozolomide in human glioma cells (2007)
  • Author(s): Tsuno T, Natsume A, Katsumata S, Mizuno M, Fujita M, Osawa H, Nakahara N, Wakabayashi T, Satoh Y, Inagaki M, Yoshida J
  • Journal Title: J Neurooncol 83 Pages: 249-58
  • Peer Reviewed
• [Journal Article] Takeuchi H, Natsume A, Wakabayashi T, Aoshima C, Shimato S, Ito M, Ishii J, Maeda Y, Hara M, Kim SU, Yoshida J (2007)
  • Author(s): Takeuchi H, Natsume A, Wakabayashi T, Aoshima C, Shimato S, Ito M, Ishii J, Maeda Y, Hara M, Kim SU, Yoshida J
  • Journal Title: Neurosci Lett 426 Pages: 69-74
  • Peer Reviewed
• [Journal Article] Human neural stem cells target and deliver therapeutic gene to experimental leptomeningeal medulloblastoma (2007)
  • Author(s): Shimato S, Natsume A, Takeuchi H, Wakabayashi T, Fujii M, Ito M, Ito S, Park IH, Bang JH, Kim SU, Yoshida J
  • Journal Title: Gene Ther 14 Pages: 1132-42
  • Peer Reviewed
• [Journal Article] Malignant transformation-related genes in meningiomas : allelic loss on 1p36 and methylation status of p73 and RASSF1A (2007)
  • Author(s): Nakane Y, Natsume A, Wakabayashi T, Oi S, Ito M, Inao S, Saito K, Yoshida J
  • Journal Title: J Neurosurg 107 Pages: 398-404
  • Peer Reviewed
• [Journal Article] Genetically heterogeneous glioblastoma recurring with disappearance of 1p/19q losses : case report (2007)
  • Author(s): Ito M, Wakabayashi T, Natsume A, Hatano H, Fujii M, Yoshida J
  • Journal Title: Neurosurgery 61
  • Peer Reviewed
• [Journal Article] The free-radical scavenger edaravone restores the differentiation of human neural precursor cells after radiation-induced oxidative stress (2007)
  • Author(s): Ishii J, Natsume A, Wakabayashi T, Takeuchi H, Hasegawa H, Kim SU, Yoshida J
  • Journal Title: Neurosci Lett 423 Pages: 225-30
  • Peer Reviewed
• [Journal Article] Efficacy of temozolomide is correlated with 1p loss and methylation of the deoxyribonucleic acid repair gene MGMT in malignant gliomas (2007)
  • Author(s): Ishii D, Natsume A, Wakabayashi T, Hatano H, Asano Y, Takeuchi H, Shimato S, Ito M, Fujii M, Yoshida J
  • Journal Title: Neurol Med Chir(Tokyo) 47 Pages: 341-9
  • NAID: 110006368605
  • Peer Reviewed
• [Journal Article] Human neural stem cells target and deliver therapeutic gene to experimental leptomeningeal medulloblastoma. (2007)
  • Author(s): Shimato S, Natsume A. Takeuchi H, Wakabayashi T, Fujii M, Ito M, Ito S, Park IH, Bang JH, Kim SU, Yoshida J.
  • Journal Title: Gene Therapy 14 Pages: 1132-42
  • Peer Reviewed
• [Journal Article] The free-radical scavenger edaravone restores the differentiation of human neural precursor cells after radiation-induced oxidative stress. (2007)
  • Author(s): Ishii J, Natsume A, Wakabayashi T, Takeuchi H, Hasegawa H, Kim SU, Yoshida J.
  • Journal Title: Neuroscience Letters 423 Pages: 225-30
  • Peer Reviewed
• [Journal Article] Intravenously transplanted human neural stem cells migrate to the injured spinal cord in adult mice in an SDF-1- and HGF-dependent manner. (2007)
  • Author(s): Takeuchi H, Natsume A, Wakabayashi T, Aoshima C, Shimato S, Ito M, Ishii J, Maeda Y, Hara M, Kim SU, Yoshida J.
  • Journal Title: Neuroscience Letters 426 Pages: 69-74
  • Peer Reviewed
• [Presentation] Image-guided epilepsy surgery (2008)
  • Author(s): 梶田泰一
  • Organizer: 11^<th> European Conference on Epilepsy and Society
  • Place of Presentation: フランス・マルセイユ
  • Year and Date: 2008-10-17
• [Presentation] 側頭葉てんかんの外科治療 側頭葉てんかん研究会 (2007)
  • Author(s): 梶田泰一
  • Place of Presentation: 名古屋
  • Year and Date: 2007-12-01
• [Presentation] 側頭葉てんかんの外科治療 (2007)
  • Author(s): 梶田 泰一
  • Organizer: 側頭葉てんかん研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-12-01