Co-transplantation of ES Cells and Bone Marrow Stromal Cells For Cerebral hemmorhage

• Project/Area Number: 19591696
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Nara Medical University
• Principal Investigator: YOSHIKAWA Masahide Nara Medical University, 医学部, 准教授 (50230701)
• Co-Investigator(Kenkyū-buntansha): NISHIMURA Fumihiko 奈良県立医科大学, 医学部, 助教 (70433331) ; WANAKA Akio 奈良県立医科大学, 医学部, 教授 (90210989)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 脳血管障害 / ES細胞 / 骨髄幹細胞 / 脳出血 / 骨髄間質幹細胞 / コラゲナーゼ / 脊髄損傷

Research Abstract

脳出血後の機能回復を目指して、ES由来神経幹細胞(ES-NSC)および骨髄間質幹細胞(BM-MSC)の共同移植を行った。脳出血モデルラットを作成し、9日目に病巣部に106個ずつを共同移植した。ES-NSC単独移植群と比較して、共同移植群に行動学的に有意な改善は認めなかったが、病巣近傍脳に多数のES細胞由来神経細胞を認めた。共培養実験では、ES-NSC分画に残存して含まれた未分化ES細胞に由来する未分化関連指標の早期消失と神経系分化指標の出現促進が観察された。

Research Products

• [Journal Article] Cotransplantation of Mouse Embryonic Stem Cells and Bone Marrow Stromal Cells Following Spinal Cord Injury Suppresses Tumor Development (2009)
  • Author(s): Matsuda Ryosuke, Yoshikawa Masahide, Nishimura Fumihiko, 他11名
  • Journal Title: Cell Transplantation 18 Pages: 39-54
  • Peer Reviewed
• [Journal Article] Treatment of Parkinson's disease model mice with allogeneic embryonic stem cells: necessity of immunosuppressive treatment for sustained improvement (2009)
  • Author(s): Toriumi Hayato, Yoshikawa Masahide, Nishimura Fumihiko, . 他7名
  • Journal Title: Neurol Res 31 Pages: 220-227
  • Peer Reviewed
• [Journal Article] Co-transplantation of Embryonic stem Cells and Bone Marrow Stromal Cells Following Spinal Cord Injury Suppresses Tumor Development (2009)
  • Author(s): Ryosuke Matsuda, et al.
  • Journal Title: Cell Transplantation Vol. 18(In press)
  • Peer Reviewed
• [Presentation] 脊髄損傷に対するES細胞移植治療の問題点 (2008)
  • Author(s): 松田良介、中瀬裕之、木村 新、西村文彦、吉川正英、榊 寿右
  • Organizer: 第23回日本脊髄外科学会
  • Place of Presentation: 宮城県宮城郡松島町 ホテル松島大観荘
  • Year and Date: 2008-06-12
• [Presentation] 脊髄損傷に対するES細胞移植治療の問題点 (2008)
  • Author(s): 松田良介, 他
  • Organizer: 第23回日本脊髄外科学会
  • Place of Presentation: 宮城県松島町ホテル松島大観荘
  • Year and Date: 2008-06-12
• [Presentation] Co-transplantation of ES Cells and Bone Marrow Stromal Cells for Spinal Cord Injury Suppresses Tumor Development (2007)
  • Author(s): Matsuda Ryosuke, Yoshikawa Masahide, 他5名
  • Organizer: 2007 Shanghai InternationalSymposium on Stem Cell Research
  • Place of Presentation: Shanghai, Republic of China
  • Year and Date: 2007-11-06
• [Presentation] Co-transplantation of ES Cells and Bone Marrow Stromal Cells for Spinal Cord Injury Suresses Tumor Develoment (2007)
  • Author(s): 松田 良介、吉川 正英
  • Organizer: 2007 Shanghai International Symposium on Stem Cell Research
  • Place of Presentation: Shanghai