Activation of intrinsic neural stem cells and induction of neural regeneration using a biologic molecule derived from cells in the brain

• Project/Area Number: 19591702
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Keio University
• Principal Investigator: NAMIKI Jun Keio University, 医学部, 講師 (20189195)
• Co-Investigator(Kenkyū-buntansha): MATSUZAKI Yumi 慶應義塾大学, 医学部, 准教授 (50338183) ; 船曳 知弘 慶應義塾大学, 医学部, 助教 (90317256)
• Co-Investigator(Renkei-kenkyūsha): FUNABIKI Tomohiro 慶應義塾大学, 医学部, 講師 (90317256)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 再生医学 / 生理活性 / 脳・神経

Research Abstract

To aim for a novel therapeutic approach that accomplishes functional recovery after brain injury, we performed basic research using experimental animals. We hypothesized that production of new neurons from intrinsic neural stem cells of the adult mammalian brain was regulated by soluble proteins secreted into the cerebrospinal fluid or parenchyma around vascular networks. We identified a biologic molecule that was secreted from endothelial cells of newly generated vasculature, and showed its effect of activation for neural stem cells.

Research Products

• [Journal Article] The neural stem/progenitor cell marker nestin is expressed in proliferative endothelial cells, but not in mature vasculature (2010)
  • Author(s): Suzuki S, Nam S, Mastuzaki Y, Okano H
  • Journal Title: J Histochem Cytochem (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] The neural stem/progenitor cell marker nestin is expressed in proliferative endothelial cells, but not in mature vasculature (2010)
  • Author(s): Suzuki S, Namiki J, Shibata S, Mastuzaki Y, Okano H
  • Journal Title: Journal of Histochemistry and Cytochemistry (印刷中)
  • Peer Reviewed
• [Presentation] 血管内皮細胞におけるネスチン発現 (2010)
  • Author(s): 並木淳, 鈴木さゆり, 松崎有未, 岡野栄之
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島市
  • Year and Date: 2010-03-18
• [Presentation] A vascular niche factor for neural stem cells (2009)
  • Author(s): Namiki J, Suzuki S Shibata S, Matsuzaki Y, Okano H
  • Organizer: The 7th Stem Cell Research Symposium
  • Place of Presentation: 東京都
  • Year and Date: 2009-05-15
• [Presentation] 骨髄由来の血管内皮前駆細胞が分泌する生理活性物質は神経幹細胞の自己複製を促進する (2008)
  • Author(s): 並木 淳
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京都
  • Year and Date: 2008-07-09
• [Presentation] 神経幹細胞の自己複製に関わる血管内皮前駆細胞のniche factor (2008)
  • Author(s): 並木 淳
  • Organizer: 第7回日本再生医療学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-13
• [Presentation] 脳損傷に対する神経再生誘導-炎症性メディエーターの制御と内在性幹細胞の動員- (2007)
  • Author(s): 並木 淳
  • Organizer: 第35回日本救急医学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2007-10-17
• [Patent(Industrial Property Rights)] 神経肝細胞に対する新規ニッシェ蛋白質 (2010)
  • Inventor(s): 並木淳
  • Industrial Property Rights Holder: 学校法人慶
  • Industrial Property Number: 2010-101415
  • Filing Date: 2010-04-26