cAMP administration on motor cortex with NT-3 secreted BMSC trarlsplantation after spinal cord injury

• Project/Area Number: 19591715
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Chiba University
• Principal Investigator: ATSUSHI Murata Chiba University, 医学部・附属病院, 准教授 (20344997)
• Co-Investigator(Kenkyū-buntansha): KAZUKI Kuniyoshi 千葉大学, 医学部・附属病院, 助教 (40375788) ; MASASHI Yamazaki 千葉大学, 大学院・医学研究院, 准教授 (50281712) ; AKIHIKO Okawa 千葉大学, 医学部・附属病院, 講師 (30312945)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 脊髄損傷 / cAMP / BMSC / BBB score / シロスタゾール / phosphodiesterase III / CREB / 大脳皮質 / hos hodiesterase III

Research Abstract

脊髄損傷の臨床応用を考え、cAMPの濃度を保つことによるフォスフォジエステラーゼ阻害薬のシロスタゾール投与による治療法を考えた。ラット脊髄損傷急性期、慢性期、予防的投与の3実験を行ったが、いずれも有意差が得られなかった。これは薬剤単独では脊髄損傷の治癒は困難であることを示唆した。

Research Products

• [Journal Article] Transplantation of Bone Marrow Stromal Cell-Derived Schwann Cell Reduces Cystic Cavity, Promotes Axonal…… (2008)
  • Author(s): Someya Y, Koda M, Dezawa M, et al.,
  • Journal Title: J Neurosurgery Spine 9(6) Pages: 600-610
• [Journal Article] Brain-derived neurotrophic factor suppresses anoikis-in duced death of Schwann cells (2008)
  • Author(s): Koda M, Someya Y, Nishio Y, Kadota R, et al.,
  • Journal Title: Neuroscience Letter 444(2) Pages: 143-147
• [Journal Article] Granulocyte colony-stimulating factor(G-CSF)mobilizes bone marrow-derived cells into injured spinal cord and promotes functional recovery after compression-induced spinal cord injury in mice (2007)
  • Author(s): Koda M, Okawa A(5^<th>), Yamazaki M(10^<th>)
  • Journal Title: Brain Research 1149 Pages: 223-31
• [Journal Article] An analysis of factors causing poor surgical outcome in patients with cervical myelopathy due to ossification of the posterior longitudinal ligament : anterior decompression with spinal fusion versus laminoplasty (2007)
  • Author(s): Masaki Y, Yamazaki M, Okawa A, Aramomi M, Hashimoto M, Koda M, Mochizuki M, Moriya H
  • Journal Title: J Spinal Disord Tech 20 Pages: 7-13
• [Journal Article] Usefulness of three-dimensional full-scale modeling of surgery for a giant cell tumor of the cervical spine (2007)
  • Author(s): Yamazaki M, Akazawa T, Okawa A, Koda M
  • Journal Title: Spinal Cord 45 Pages: 250-253
• [Journal Article] Traumatic C6-7 subluxation with anomalous course of vertebral arteries treated with pedicle screw/rod fixation. Case report (2007)
  • Author(s): Yamazaki M, Koshi T, Mannoji C, Okawa A, Koda M
  • Journal Title: J Neurosurg Spine 7(1) Pages: 65-70
• [Journal Article] Usefulness of 3-dimensional full-scale modeling for preoperative simulation of surgery in a patient with old unilateral cervical fracture-dislocation (2007)
  • Author(s): Yamazaki M, Okawa A, Akazawa T, Koda M
  • Journal Title: Spine 32(18)
• [Journal Article] Granulocyte colony-stimulating factor attenuates neuronal death and promotes functional recovery after spinal cord injury in mice (2007)
  • Author(s): Nishio Y, Koda M, Canada T, Someya Y, Kadota R, Mannoji C, Miyashita T, Okada S, Okawa A, Moriya H, Yamazaki M
  • Journal Title: J Neuropathol Exp Neurol 66(8) Pages: 724-31
• [Journal Article] Adenovirus vector-mediated ex vivo gene transfer of brain-derived neurotrophic factor to bone marrow stromal cells promotes axonal regeneration after transplantation in completely transected adult rat spinal cord (2007)
  • Author(s): Koda M, Kamada T, Hashimoto M, Murakami M, Shirasawa H, Sakao S, Ino H, Yoshinaga K, Koshizuka S, Moriya H, Yamazaki M
  • Journal Title: Eur Spine J(Epub ahead of print) Sep 21
• [Presentation] ラット脊髄損傷に対するシロスタゾール投与の有効性の検討 (2008)
  • Author(s): 山崎正志
  • Organizer: 脊髄障害学会
  • Place of Presentation: 札幌
  • Year and Date: 2008-11-06
• [Presentation] ラット脊髄圧挫損傷モデルにおけるbFGF徐放ゼラチンハイドロゲル移植の検討 (2008)
  • Author(s): 古矢丈雄、橋本将行、国府田正雄、大河昭彦、山崎正志、松瀬大
  • Place of Presentation: 札幌
  • Year and Date: 2008-11-06
• [Presentation] ラット脊髄損傷に対するシロスタゾール投与の有用性の検討 (2008)
  • Author(s): 林浩一、國府田正雄 大河昭彦、村田淳、山崎正志
  • Organizer: 日本脊髄障害学会
  • Place of Presentation: 札幌
  • Year and Date: 2008-11-06
• [Presentation] ラット脊髄圧挫損傷モデルにおけるグリア前躯細胞由来アストロサイト移植の検討 (2008)
  • Author(s): 古矢丈雄、橋本将行、国府田正雄、村田淳、大河昭彦、山崎正志、高橋和久
  • Place of Presentation: 札幌
  • Year and Date: 2008-10-23
• [Presentation] ラット脊髄圧挫損傷モデルにおけるbFGF徐放ゼラチンハイドロゲル移植の検討 (2008)
  • Author(s): 古矢丈雄、橋本将行、国府田正雄、村田淳、大河昭彦、山崎正志、出澤真理、松瀬大、田畑泰彦、高橋和久
  • Place of Presentation: 京都
  • Year and Date: 2008-10-23
• [Presentation] マウス脊髄圧挫損傷モデルにおけるヒト骨髄問質細胞由来神経幹細胞移植の検討 (2007)
  • Author(s): 萬納寺誓人、国府田正雄、出沢真理、大河昭彦、山崎正志
  • Place of Presentation: 浜松
  • Year and Date: 2007-10-25
• [Presentation] ラット脊髄圧挫モデルにおける骨髄間質細胞移植とRhoキナーゼ阻害薬の併用による治療効果 (2007)
  • Author(s): 古矢丈雄、橋本将行、国府田正雄、山崎正志、山下俊英、守屋秀繁
  • Place of Presentation: 浜松
  • Year and Date: 2007-10-25
• [Presentation] ラット脊髄圧挫損傷モデルにおける穎粒球コロニー刺激因子(Granulocyte Colony stimulating Factor : G-CSF)の血管系における効果 (2007)
  • Author(s): 辺純子、国府田正雄、橋本将行、門田領、大河昭彦、山崎正志
  • Place of Presentation: 浜松
  • Year and Date: 2007-10-25
• [Presentation] ラット脊髄圧挫モデルにおける骨髄間質細胞移植とRhoキナーゼ阻害薬の併用による治療効果 (2007)
  • Author(s): 古矢 丈雄, 橋本 将行, 国府 田正雄, 大河 昭彦, 村田 淳、山崎 正志
  • Organizer: 日本整形外科学会基礎学術集会
  • Place of Presentation: 浜松
  • Year and Date: 2007-10-25