Molecular therapy for bone and soft tissue sarcomas targeting a new cancer-associated protein, C7orf24

• Project/Area Number: 19591719
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kyoto University
• Principal Investigator: NAKAYAMA Tomitaka Kyoto University, 医学研究科, 講師 (80335273)
• Co-Investigator(Kenkyū-buntansha): 戸口田 淳也 京都大学, 再生医科学研究所, 教授 (40273502)
• Co-Investigator(Renkei-kenkyūsha): TOGUCHIDA Junya 京都大学, 再生医科学研究所, 教授 (40273502)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: C7orf24 / 骨軟部肉腫 / 分子標的治療

Research Abstract

膀胱癌の治療標的分子として同定されたC7orf24の骨肉腫における意義を明らかにすることを目的とした. C7orf24は骨肉腫腫瘍組織で高発現していたが、発現導入実験では、明らかな表現型を誘導できず、発現抑制実験では特定の細胞株では著しい形態変化を導いたが、普遍的な現象ではなかった. アポトーシス等の細胞死との関連も検出されず、C7orf24の骨肉腫における治療標的としての明確な意義を示す結果は得られなかった.

Research Products

• [Journal Article] Inverse correlation of the up-regulation of FZD10 expression and the activation of beta-catenin in synchronous colorectal tumors (2009)
  • Author(s): Nagayama S, Yamada E, Kohno Y, Aoyama T, Fukukawa C, Kubo H, Watanabe G, Katagiri T, Nakamura Y, Sakai Y, Toguchida J.
  • Journal Title: Cancer Sci 100(3) Pages: 405-12
  • Peer Reviewed
• [Journal Article] Involvement of ERK activation in human osteosarcoma cell resistance to the HDAC inhibitor FK228 (2009)
  • Author(s): Matsubara H, Watanabe M, Imai T, Yui Y, Mizushima Y, Hiraumi Y, Kamitsuji Y, Watanabe KI, Nishijo K, Toguchida J, Nakahata T, Adachi S.
  • Journal Title: J Pharmacol Exp Ther 328(3) Pages: 839-48
  • Peer Reviewed
• [Journal Article] Activation of the non-canonical Dvl-Rac1-JNK pathway by Frizzled homologue 10 in human synovial sarcoma (2009)
  • Author(s): Fukukawa C, Nagayama S, Tsunoda T, Toguchida J, Nakamura Y, Katagiri T.
  • Journal Title: Oncogene 28(8) Pages: 1110-20
  • Peer Reviewed
• [Journal Article] Induction of autophagy in malignant rhabdoid tumor cells by the histone deacetylase inhibitor FK228 through AIF translocation (2008)
  • Author(s): Watanabe M, Adachi S, Matsubara H, Imai T, Yui Y, Mizushima Y, Hiraumi Y, Watanabe KI, Kamitsuji Y, Toyokuni SY, Hosoi H, Sugimoto T, Toguchida J, Nakahata T.
  • Journal Title: Int J Cancer 124(1) Pages: 55-67
  • Peer Reviewed
• [Journal Article] Prognostic factors of pulmonary metastasectomy for osteosarcomas of the extremities (2008)
  • Author(s): Chen F, Miyahara R, Bando T, Okubo K, Watanabe K, Nakayama T, Toguchida J, Date H.
  • Journal Title: Eur J Cardiothorac Surg 34(6) Pages: 1235-9
  • Peer Reviewed
• [Journal Article] Validation of radiographic response evaluation criteria of preoperative chemotherapy for bone and soft tissue sarcomas : Japanese Orthopaedic Association Committee on Musculoskeletal Tumors Cooperative Study (2008)
  • Author(s): Ueda T, Naka N, Araki N, Ishii T, Tsuchiya H, Yoshikawa H, Mochizuki K, Tsuboyama T, Toguchida J, Ozaki T, Murata H, Kudawara I, Tanaka K, Iwamoto Y, Yazawa Y, Kushida K, Otsuka T, Sato K.
  • Journal Title: J Orthop Sci 13(4) Pages: 304-12
  • Peer Reviewed
• [Journal Article] Neuronal differentiation of synovial sarcoma and its therapeutic application (2008)
  • Author(s): Ishibe T, Nakayama T, Aoyama T, Nakamura T, Toguchida J.
  • Journal Title: Clin Orthop Rel Res 466(9) Pages: 2147-55
  • Peer Reviewed
• [Journal Article] Natural course of desmoid-type fibromatosis (2008)
  • Author(s): Nakayama T, Tsuboyama T, Toguchida J, Hosaka T, Nakamura T.
  • Journal Title: J Orthop Sci 13(1) Pages: 51-5
  • NAID: 10024138969
  • Peer Reviewed
• [Journal Article] Radioimmunotherapy of human synovial sarcoma using a monoclonal antibody against FZD10 (2008)
  • Author(s): Furukawa C, Hanaoka H, Nagayama S, Tsunoda T, Toguchida J, Endo K, Nakamura Y, Katagiri T.
  • Journal Title: Cancer Sci 99(2) Pages: 432-40
  • Peer Reviewed
• [Journal Article] Prognostic implication of SYT-SSX fusion type in synovial sarcoma : A multi-institutional retrospective analysis in Japan (2008)
  • Author(s): Takenaka S, Ueda T, Naka N, Araki N, Hashimoto N, Myoui A, Ozaki T, Nakayama T, Toguchida J, Tanaka K, Iwamoto Y, Matsumine A, Uchida A, Ieguchi M, Kaya M, Wada T.
  • Journal Title: Oncology Report 19(2) Pages: 467-476
  • Peer Reviewed
• [Journal Article] Prognostic implication of SYT-SSX fusion type in synovial sarcoma : A multi-institutional retrospective analysis in Japan (2008)
  • Author(s): Takenaka S, et al.
  • Journal Title: Oncology Report 19 Pages: 467-476
  • Peer Reviewed
• [Journal Article] Neuronal differentiation of synovial sarcoma and its therapeutic application (2008)
  • Author(s): Ishibe T, et al.
  • Journal Title: Clin Orthop Rel Res 466 Pages: 2147-2155
  • Peer Reviewed
• [Journal Article] Validation of radiographic response evaluation criteria of preoperative chemotherapy for bone and soft tissue sarcomas : Japanese Orthopaedic Association Committee on Musculoskeletal Tumors Cooperative Study (2008)
  • Author(s): Ueda T, et al.
  • Journal Title: J Orthop Sci 13 Pages: 304-312
  • Peer Reviewed
• [Journal Article] Prognostic factors of pulmonary metastasectomy for osteosarcomas of the extremities (2008)
  • Author(s): Chen F, et al.
  • Journal Title: Eur J Cardiothorac Surg 34 Pages: 1235-1239
  • Peer Reviewed
• [Journal Article] A novel tumor-related protein, C7orf24, identified by proteome differential display of bladder urothelial carcinoma (2007)
  • Author(s): Kageyama S, Iwaki H, Inoue H, Isono T, Yuasa T, Nogawa M, Maekawa T, Ueda M, Kajita Y, Ogawa O, Toguchida, J, Yoshiki T.
  • Journal Title: Proteomics Clin Appl 1 Pages: 192-199
  • Peer Reviewed
• [Journal Article] In vitro transformation of mesenchymal stem cells by oncogenic H-rasVal12. (2007)
  • Author(s): Shima Y, et. al.
  • Journal Title: Biochem Biophys Res Commun 353 Pages: 60-66
  • Peer Reviewed
• [Journal Article] A novel tumor-related protein, C7orf24, identified by proteome differential display of bladder urothelial carcinoma. (2007)
  • Author(s): Kageyama S, et. al.
  • Journal Title: Proteomics Clin Appl 1 Pages: 192-199
  • Peer Reviewed
• [Journal Article] A Case of a Ewing Sarcoma Family Tumor in the Urinary Bladder After Treatment for Acute Lymphoblastic Leukemia. (2007)
  • Author(s): Osone S, et. al.
  • Journal Title: J Pediatr Hematol Oncol 29 Pages: 841-844
  • Peer Reviewed
• [Journal Article] Prognostic implication of SYT-SSX fusion type in synovial sarcoma: Amulti-institutional retrospective analysis in Japan. (2007)
  • Author(s): Takenaka S, et. al.
  • Journal Title: Oncology Report 19 Pages: 467-476
  • Peer Reviewed
• [Journal Article] Radioimmunotherapy of human synovial sarcoma using a monoclonal antibody against FZD10. (2007)
  • Author(s): Fukukawa C, et. al.
  • Journal Title: Cancer Sci 99 Pages: 432-440
  • Peer Reviewed
• [Journal Article] Molecular genetics of sarcomas : Applications to diagnosses and theray
  • Author(s): Toguchida J, Nakayama T.
  • Journal Title: Cancer Sci (in press)
  • Peer Reviewed
• [Presentation] 網羅的解析からの骨軟部腫瘍へのアプローチ (2008)
  • Author(s): 戸口田淳也
  • Organizer: 第2回東京医科歯科大学硬組織疾患ゲノムセンターシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-11-25
• [Presentation] 体外処理骨を用いた悪性骨腫瘍切除後再建 (2008)
  • Author(s): 中山富貴、戸口田淳也、他
  • Organizer: 第17回小児固形腫瘍研究会
  • Place of Presentation: 京都
  • Year and Date: 2008-11-21
• [Presentation] 肉腫における転移関連新規因子C7059の機能解析 (2008)
  • Author(s): 布留守敏、中山富貴、戸口田淳也、他
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] 肉腫において組織幹細胞としての特質は癌幹細胞としての特質に連関するものか (2008)
  • Author(s): 戸口田淳也、中山富貴、他
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] 肉腫におけるras遺伝子の変異解析 (2008)
  • Author(s): 金永輝、中山富貴、戸口田淳也、他
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] 大腸癌にて高発現している新規遺伝子の免疫組織化学染色による発現解析 (2008)
  • Author(s): 長山聡、戸口田淳也、他
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] 肉腫転移関連因子C7059の機能解析 (2008)
  • Author(s): 布留守敏、中山富貴、戸口田淳也、他
  • Organizer: 第23回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-10-24
• [Presentation] 肉腫転移関連因子C70059の機能解析 (2008)
  • Author(s): 布留守敏, 他.
  • Organizer: 第23回日本整形外科学会基礎学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-10-24
• [Presentation] 分化誘導プロファイルを用いた肉腫起源細胞へのアプローチ (2008)
  • Author(s): 青山朋樹、中山富貴、戸口田淳也、他
  • Organizer: 第41回日本整形外科学会骨軟部腫瘍学術集会
  • Place of Presentation: 浜松
  • Year and Date: 2008-07-18
• [Presentation] 多発骨転移を生じた粘液型脂肪肉腫の3例 (2008)
  • Author(s): 中山富貴, 他.
  • Organizer: 第41回日本整形外科学会骨軟部腫瘍学術集会
  • Place of Presentation: 浜松
  • Year and Date: 2008-07-18
• [Presentation] 遺伝子発現プロファイルからみた骨・軟部腫瘍の起源・分類・診断 (2008)
  • Author(s): 戸口田淳也
  • Organizer: 第41回日本整形外科学会骨軟部腫瘍学術集会
  • Place of Presentation: 浜松
  • Year and Date: 2008-07-17
• [Presentation] 肉腫転移関連因子C7059の機能解析および治療標的としての可能性の探索 (2008)
  • Author(s): 布留守敏、中山富貴、戸口田淳也、他
  • Organizer: 第41回日本整形外科学会骨軟部腫瘍学術集会
  • Place of Presentation: 浜松
  • Year and Date: 2008-07-17
• [Presentation] 大腿骨遠位部骨腫瘍に対する非蝶番型人工膝関節の使用経験 (2008)
  • Author(s): 仲俣岳晴, 他.
  • Organizer: 第41回日本整形外科学会骨軟部腫瘍学術集会
  • Place of Presentation: 浜松
  • Year and Date: 2008-07-17
• [Presentation] 肉腫幹細胞と組織幹細胞の接点 (2008)
  • Author(s): 戸口田淳也
  • Organizer: 治療開発を目指した最前線セミナー2Cancer Stem Cell~がん幹細胞をターゲットとした治療の開発に向けて~
  • Place of Presentation: 東京
  • Year and Date: 2008-06-24
• [Presentation] 癌幹細胞探索のための細胞イメージングの可能性 (2008)
  • Author(s): 戸口田淳也
  • Organizer: 第47回日本生体医工学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-05-08
• [Presentation] Snail is negative regulator controlling the construction of epithelial structures in SS (2007)
  • Author(s): Kohno T, Nakayama T, Toguchida J, et.al.
  • Organizer: 第13回(CTOS)
  • Place of Presentation: Seattle
  • Year and Date: 2007-11-02
• [Presentation] Multidirectional differentiation property like mesenchymal stem cells of osteosarcomas in vitro and in vivo (2007)
  • Author(s): Otsuka S, Nakayama T, Toguchida J, et.al.
  • Organizer: 第13回(CTOS)
  • Place of Presentation: Seattle
  • Year and Date: 2007-11-02
• [Presentation] Isolation and functional analysis of a novel protein associated with the distant metastases of spindle cell sarcomas (2007)
  • Author(s): Furu M, Nakayama T, Toguchida J, et.al.
  • Organizer: 第13回(CTOS)
  • Place of Presentation: Seattle
  • Year and Date: 2007-11-02
• [Presentation] Isolation and functional analysis of a novel protein associated with the distant metastases of spindle cell sarcomas. (2007)
  • Author(s): Moritoshi Furu, et. al.
  • Organizer: 13th Annual Meeting of Connective Tissue Oncology Society
  • Place of Presentation: Seattle
  • Year and Date: 2007-11-02
• [Presentation] 滑膜肉腫の上皮構造形成におけるCLD7、ELF3およびSnailの関連性について (2007)
  • Author(s): 光野芳樹、中山富貴、戸口田淳也、他
  • Organizer: 第22回日本整形外科学会基礎学術集会
  • Place of Presentation: 浜松
  • Year and Date: 2007-10-26
• [Presentation] 紡錘形軟部肉腫における生命予後因子C7059と細胞浸潤能の解析 (2007)
  • Author(s): 布留守敏、戸口田淳也、他
  • Organizer: 第22回日本整形外科学会基礎学術集会
  • Place of Presentation: 浜松
  • Year and Date: 2007-10-25
• [Presentation] Snail is negative regulator controlling the construction of epithelial structures in SS (2007)
  • Author(s): Kohno Y, Nakayama T, Toguchida J, et.al.
  • Organizer: 第66回日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-04
• [Presentation] Isolation and functional analysis of a novel protein associated with the distant metastases of spindle cell sarcomas (2007)
  • Author(s): Furu M, Nakayama T, Toguchida J, et.al.
  • Organizer: 第66回日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-04
• [Presentation] Some osteosarcomas have multidirectional differentiation potential like mesenchymal stem cells in vitro and in vivo (2007)
  • Author(s): Otsuka S, Nakayama T, Toguchida J, et.al.
  • Organizer: 第66回日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-03
• [Presentation] イットリウム90-抗FZD10モノクローナル抗体による滑膜肉腫抗体治療の検討 (2007)
  • Author(s): 福川千香子、戸口田淳也、他
  • Organizer: 第66回日本癌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-03
• [Presentation] Relationship among CLDN7, ELF3, and Snail in the formation of the epithelial structures in synovial sarcomas (2007)
  • Author(s): Kohno Y, Nakayama T, Toguchida J, et.al.
  • Organizer: 第5回SIROT
  • Place of Presentation: Marrakech
  • Year and Date: 2007-08-31
• [Presentation] Isolation and functional analysis of a novel protein identified as the prognostic factor in spindle cell sarcomas (2007)
  • Author(s): Furu M, Nakayama T, Toguchida J, et.al.
  • Organizer: 第5回SIROT
  • Place of Presentation: Marrakech
  • Year and Date: 2007-08-31
• [Presentation] 滑膜肉腫の上皮構造形成におけるCLDN7、ELF3およびSnailの関連性について (2007)
  • Author(s): 光野芳樹、中山富貴、戸口田淳也、他
  • Organizer: 第40回日本整形外科学会骨軟部腫瘍学術集会
  • Place of Presentation: 甲府
  • Year and Date: 2007-07-12
• [Presentation] 培養骨肉腫細胞のもつ間葉系幹細胞様の多分化能 (2007)
  • Author(s): 大塚聖視、中山富貴、戸口田淳也、他
  • Organizer: 第40回日本整形外科学会骨軟部腫瘍学術集会
  • Place of Presentation: 甲府
  • Year and Date: 2007-07-12
• [Presentation] 紡錘形軟部肉腫における生命予後関連因子としての新規遺伝子C7059の単離と機能解析 (2007)
  • Author(s): 布留守敏、中山富貴、戸口田淳也、他
  • Organizer: 第40回日本整形外科学会骨軟部腫瘍学術集会
  • Place of Presentation: 甲府
  • Year and Date: 2007-07-12
• [Presentation] 網羅的遺伝子発現解析からの紡錘形細胞肉腫における生命予後関連因子の単離 (2007)
  • Author(s): 戸口田淳也、中山富貴、他
  • Organizer: 第40回日本整形外科学会骨軟部腫瘍学術集会
  • Place of Presentation: 甲府
  • Year and Date: 2007-07-12
• [Presentation] Some osteosarcomas have multidirectional differentiation potential suggested that these cells may derive directly from mesenchymal stem cells (2007)
  • Author(s): Otsuka S, Nakayama T, Toguchida J, et.al.
  • Organizer: 第5回ISSCR
  • Place of Presentation: Cairns
  • Year and Date: 2007-06-19