Search for useful biomarkers in bone and soft tissue tumors using global expression analysis

• Project/Area Number: 19591743
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East
• Principal Investigator: KAWAI Akira National Cancer Center Research Institute and Research Center for Innovative Oncology, National Cancer Center Hospital East, 研究所及び東病院臨床開発センター, 研究員 (90252965)
• Co-Investigator(Kenkyū-buntansha): KONDOU Tadashi 国立がんセンター, 研究所及び東病院臨床開発センター, プロジェクトリーダー (30284061) ; ICHIKAWA Hitoshi 国立がんセンター, 研究所及び東病院臨床開発センター, プロジェクトリーダー (30201924)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 骨軟部腫瘍 / バイオマーカー / 網羅的発現解析 / タンパク質 / 遺伝子 / 悪性骨軟部腫瘍 / 二次元電気泳動

Research Abstract

In the current study a number of novel prognostic factors and factors determining the biological characteristics of gastrointestinal stromal tumor (GIST), Ewing's sarcoma, and synovial sarcoma were identified. For GIST, a novel prognostic marker "pfetin" was identified on 2D-DIGE, and its usefulness was verified by performing immunostaining for samples from multiple facilities. As for Ewing's sarcoma, "nucleophosmin" was identified as a novel prognostic marker.

Research Products

• [Journal Article] Expression of Bone Morphogenetic Proteins in Giant Cell Tumor of Bone. (2009)
  • Author(s): Naoko Kudo, Akira Ogose, Takashi Ariizumi, Hiroyuki Kawashima, Tetsuo Hotta, Hiroshi Hatano, Tetsuro Morita, Masaki Nagata, Yukie Siki, Akira Kawai, Yuko Hotta, Makiko Hoshino, Naoto Endo.
  • Journal Title: Anticancer Research 29 Pages: 2219-2226
  • Peer Reviewed
• [Journal Article] Multiinstitutional phase II study of neoadjuvant chemotherapy for osteosarcoma (NECO study) in Japan: NECO-93J and NECO-95J. (2009)
  • Author(s): Yukihide Iwamoto, Kazuhiro Tanaka, Kazuo Isu, Akira Kawai, Shin-ichiro Tatezaki, Takeshi Ishii, Kazuyoshi Kushida, Yasuo Beppu, Masamichi Usui, Akio Tateishi, Kiyoo Furuse, Takeshi Minamizaki, Noriyoshi Kawaguchi, Shinya Yamawaki.
  • Journal Title: J Orthop Sci 14 Pages: 397-404
  • NAID: 10025354246
  • Peer Reviewed
• [Journal Article] Nucleophosmin as a candidate prognostic biomarker of Ewing's sarcoma revealed by proteomics. (2009)
  • Author(s): Kazutaka Kikuta, Naobumi Tochigi, Tadakazu Shimoda, Hiroki Yabe, Hideo Morioka, Yoshiaki Toyama, Ako Hosono, Yasuo Beppu, Akira Kawai, Setsuo Hirohashi, Tadashi Kondo.
  • Journal Title: Clin Cancer Res 15 Pages: 2885-2894
  • Peer Reviewed
• [Journal Article] Anatomic site-specific proteomic signatures of gastrointestinal stromal tumors. (2009)
  • Author(s): Yoshiyuki Suehara, Kazutaka Kikuta, Robert Nakayama, Kiyonaga Fujii, Hitoshi Ichikawa, Tatsuhiro Shibata, Kunihiko Seki, Tadashi Hasegawa, Masahiro Gotoh, Naobumi Tochigi, Tadakazu Shimoda, Yasuhiro Shimada, Takeshi Sano, Yasuo Beppu, Hisashi Kurosawa, Setsuo Hirohashi, Akira Kawai, Tadashi Kondo.
  • Journal Title: Proteomics Clin Appl. 3 Pages: 584-596
  • Peer Reviewed
• [Journal Article] GST-P1 as a histological biomarker of synovial sarcoma revealed by proteomics. (2009)
  • Author(s): Yoshiyuki Suehara, Kazutaka Kikuta, Robert Nakayama, Naobumi Tochigi, Kunihiko Seki, Hitoshi Ichikawa, Kiyonaga Fujii, Tadashi Hasegawa, Tadakazu Shimoda, Hisashi Kurosawa, Hirokazu Chuman, Yasuo Beppu, Akira Kawai, Setsuo Hirohashi, Tadashi Kondo.
  • Journal Title: Proteomics Clin Appl. 3 Pages: 623-634
  • Peer Reviewed
• [Journal Article] Functional genome screen for therapeutic targets of osteosarcoma. (2009)
  • Author(s): Umio Yamaguchi, Kazufumi Honda, Reiko Satow, Eisuke Kobayashi, Robert Nakayama, Hitoshi Ichikawa, Ayako Shoji, Miki Shitashige, Mari Masuda, Akira Kawai, Hirokazu Chuman, Yukihide Iwamoto, Setsuo Hirohashi, Tesshi Yamada.
  • Journal Title: Cancer Sci 100 Pages: 2268-2274
  • NAID: 10027197838
  • Peer Reviewed
• [Journal Article] 骨腫瘍の診断. (2009)
  • Author(s): 川井章
  • Journal Title: 病理と臨床 27 Pages: 106-113
• [Journal Article] 全国骨・軟部腫瘍登録について (2009)
  • Author(s): 川井章
  • Journal Title: MB Orthop 22 Pages: 143-149
• [Journal Article] Expression of Bone Morphogenetic Proteins in Giant Cell Tumor of Bone (2009)
  • Author(s): Naoko Kubo, et al
  • Journal Title: Anticancer Research 29 Pages: 2219-2226
  • Peer Reviewed
• [Journal Article] Multiinstitutional phase II study of neoadjuvant chemotherapy for osteosarcoma(NECO study)in Japan : NECO-93J and NECO-95J (2009)
  • Author(s): Yukihide Iwamoto, et al.
  • Journal Title: J Orthop Sci 14 Pages: 397-404
  • Peer Reviewed
• [Journal Article] Nucleophosmin as a candidate prognostic biomarker of Ewing's sarcoma revealed by proteomics (2009)
  • Author(s): Kazutaka Kikuta, et al.
  • Journal Title: Clin Cancer Res 15 Pages: 2885-2894
  • Peer Reviewed
• [Journal Article] Anatomic site-specific proteomic signatures of gastrointestinal stromal tumors (2009)
  • Author(s): Yoshiyuki Suehara, et al.
  • Journal Title: Proteomics Clin Appl 3 Pages: 584-596
  • Peer Reviewed
• [Journal Article] GST-P1 as a histological biomarker of synovial sarcoma revealed by proteomics (2009)
  • Author(s): Yoshiyuki Suehara, et al.
  • Journal Title: Proteomics Clin Appl 3 Pages: 623-634
  • Peer Reviewed
• [Journal Article] Functional genome screen for therapeutic targets of osteosarcoma (2009)
  • Author(s): Umio Yamaguchi, et al.
  • Journal Title: Cancer Sci 100 Pages: 2268-2274
  • Peer Reviewed
• [Journal Article] Association of a missense single nucleotide polymorphism, Cys1367Arg of the WRN gene, with the risk of bone and soft tissue sarcomas in Japan. (2008)
  • Author(s): Robert Nakayama, Yasunori Sato, Mitsuko Matsutani, Hideki Ogino, Fumihiko Nakatani, Hirokazu Chuman, Yasuo Beppu, Hideo Morioka, Hiroo Yabe, Hiroshi Hirose, Haruhiko Sugimura, Hiromi Sakamoto, Tsutomu Ohta, Yoshiaki Toyama, Teruhiko Yoshida, Akira Kawai.
  • Journal Title: Cancer Sci 99 Pages: 333-339
  • NAID: 10024002483
  • Peer Reviewed
• [Journal Article] Pfetin as a prognostic biomarker of gastrointestinal stromal tumors revealed by proteomics. (2008)
  • Author(s): Yoshiyuki Suehara, Tadashi Kondo, Kunihiko Seki, Tatsuhiro Shibata, Kiyonaga Fujii, Masahiro Gotoh, Tadashi Hasegawa, Yasuhiro Shimada, Mitsuru Sasako, Tadakazu Shimoda, Hisashi Kurosawa, Yasuo Beppu, Akira Kawai, Setsuo Hirohashi.
  • Journal Title: Clin Cancer Res 14 Pages: 1707-1717
  • Peer Reviewed
• [Journal Article] Segregated graft-versus-tumor effect between CNS and non-CNS lesions of Ewing's sarcoma family of tumors. (2008)
  • Author(s): Ako Hosono, Atsushi Makimoto, Akira Kawai, Yoichi Takaue.
  • Journal Title: Bone Marrow Transplantation 41 Pages: 1067-1068
  • Peer Reviewed
• [Journal Article] Global protein-expression analysis of bone and soft tissue sarcomas. (2008)
  • Author(s): Akira Kawai, Tadashi Kondo, Yoshiyuki Suehara, Kazutaka Kikuta, Setsuo Hirohashi.
  • Journal Title: Clin Orthop Relat Res 466 Pages: 2099-2106
  • Peer Reviewed
• [Journal Article] Distinct gene expression-defined classes of gastrointestinal stromal tumor. (2008)
  • Author(s): Umio Yamaguchi, Robert Nakayama, Kazufumi Honda, Hitoshi Ichikawa, Tadashi Hasegawa, Miki Shitashige, Masaya Ono, Ayako Shoji, Tomohiro Sakuma, Hideya Kuwabara, Yasuhiro Shimada, Mitsuru Sasako, Tadakazu Shimoda, Akira Kawai, Setsuo Hirohashi, Tesshi Yamada.
  • Journal Title: J Clin Oncol 26 Pages: 4100-4108
  • Peer Reviewed
• [Journal Article] Myxoinflammatory fibroblastic sarcoma. (2008)
  • Author(s): Eisuke Kobayashi, Akira Kawai, Makoto Endo, Yoshiyuki Suehara, Ken Takeda, Fumihiko Nakatani, Takayuki Asano, Minoru Sakuraba, Hirokazu Chuman, Kunihiko Seki, Yasuo Beppu.
  • Journal Title: J Orthop Sci 13 Pages: 566-571
  • NAID: 10025353332
  • Peer Reviewed
• [Journal Article] Prediction of response and prognostic factors for Ewing family of tumors in a low incidence population. (2008)
  • Author(s): Kan Yonemori, Umio Yamaguchi, Masayuki Kaneko, Hajime Uno, Masahiro Takeuchi, Masashi Ando, Yasuhiro Fujiwara, Ako Hosono, Atsushi Makimoto, Tadashi Hasegawa, Ryohei Yokoyama, Fumihiko Nakatani, Akira Kawai, Yasuo Beppu, Hirokazu Chuman.
  • Journal Title: J Cancer Res Clin Oncol 134 Pages: 389-95
  • Peer Reviewed
• [Journal Article] 骨・軟部腫瘍の臨床診断 (2008)
  • Author(s): 川井章
  • Journal Title: 日整会誌 82 Pages: 536-548
  • NAID: 10024576050
  • Peer Reviewed
• [Journal Article] 骨・軟部腫瘍に対する補助化学療法. (2008)
  • Author(s): 小林英介、川井章.
  • Journal Title: 腫瘍内科 2(6) Pages: 510-516
• [Journal Article] Association of a missense single nucleotide polymorphism, Cys1367Arg of the WRN gene, with the risk of bone and soft tissue sarcomas in Japan (2008)
  • Author(s): Robert Nakayama, Akira Kawai, et al.
  • Journal Title: Cancer Sci 99 Pages: 333-339
  • NAID: 10024002483
  • Peer Reviewed
• [Journal Article] Pfetin as a prognostic biomarker of gastrointestinal stromal tumors revealed by proteomics (2008)
  • Author(s): Yoshiyuki Suehara, Akira Kawai, et al.
  • Journal Title: Clin Cancer Res 14 Pages: 1707-1717
  • Peer Reviewed
• [Journal Article] Segregated graft-versus-tumor effect between CNS and non-CNS lesions of Ewing's sarcoma family of tumors (2008)
  • Author(s): Ako Hosono, Akira Kawai, et al.
  • Journal Title: Bone Marrow Transplantation 41 Pages: 1067-1068
  • Peer Reviewed
• [Journal Article] Global protein-expression analysis of bone and soft tissue sarcomas (2008)
  • Author(s): Akira Kawai, et al.
  • Journal Title: Clin Orthop Relat Res 466 Pages: 2099-2106
  • Peer Reviewed
• [Journal Article] Distinct gene expression-defined classes of gastrointestinal stromaltumor (2008)
  • Author(s): Umio Yamaguchi, Akira Kawai, et al.
  • Journal Title: J Clin Oncol 26 Pages: 4100-4108
  • Peer Reviewed
• [Journal Article] Clear cell sarcoma of tendons and aponeuroses: A study of 75 patients. (2007)
  • Author(s): Akira Kawai, Ako Hosono, Robert Nakayama, Akihiko Matsumine, Seiichi Matsumoto, Takafumi Ueda, Hiroyuki Tsuchiya, Yasuo Beppu, Hideo Morioka, Hiroo Yabe.
  • Journal Title: Cancer 109 Pages: 109-116
  • Peer Reviewed
• [Journal Article] Dysadherin expression as a significant prognostic factor and as a determinant of histologic features in synovial sarcoma: Special reference to its inverse relationship with E-cadherin expression. (2007)
  • Author(s): Teiyu Izumi, Yoshinao Oda, Tadashi Hasegawa, Yukihiro Nakanishi, Akira Kawai, Hiroshi Sonobe, Tomonari Takahira, Chikashi Kobayashi, Hidetaka Yamamoto, Sadafumi Tamiya, Setsuo Hirohashi, Yukihide Iwamoto, Masazumi Tsuneyoshi.
  • Journal Title: Am J Surg Pathol 31 Pages: 85-94
  • Peer Reviewed
• [Journal Article] Clinical outcome of patients with Ewing sarcoma family of tumors of bone in Japan. (2007)
  • Author(s): Hideto Obata, Takafumi Ueda, Akira Kawai, Takeshi Ishii, Toshifumi Ozaki, Satoshi Abe, Kazuhiro Tanaka, Hiroyuki Tsuchiya, Akihiko Matsumine, Hiroo Yabe.
  • Journal Title: Cancer 109 Pages: 767-775
  • Peer Reviewed
• [Journal Article] Construct validity of the Enneking score for measuring function in patients with malignant or aggressive benign tumours of the upper limb. (2007)
  • Author(s): Takuro Wada, Akira Kawai, K Ihara, M Sasaki, T Sonoda, T Imaeda, T Yamashita.
  • Journal Title: J Bone Joint Surg 89-B Pages: 659-663
  • Peer Reviewed
• [Journal Article] Gene expression analysis of soft tissue sarcomas: Characterization and reclassification of malignant fibrous histiocytoma. (2007)
  • Author(s): Robert Nakayama, Takeshi Nemoto, Hiro Takahashi, Tsutomu Ohta, Akira Kawai, Kunihiko Seki, Teruhiko Yoshida, Yoshiaki Toyama, Hitoshi Ichikawa, Tadashi Hasegawa.
  • Journal Title: Modern Pathology 20 Pages: 749-759
  • Peer Reviewed
• [Journal Article] New approach for assessing vascular distribution within bone tumors using dynamic contrast-enhanced MRI. (2007)
  • Author(s): Yukio Kawakami, Toshiyuki Kunisada, Shinsuke Sugihara, Atsushi Ono, Keiichiro Nishida, Nobuhiro Abe, Akira Kawai, Kazuo Fujiwara, Yuki Morimoto, Toshifumi Ozaki.
  • Journal Title: J Cancer Res Clin Oncol 133 Pages: 697-703
  • Peer Reviewed
• [Journal Article] Prognostic implications of glucose transporter protein-1 (Glut-1) overexpression in bone and soft-tissue sarcomas. (2007)
  • Author(s): Makoto Endo, Ukihide Tateishi, Kunihiko Seki, Umio Yamaguchi, Fumihiko Nakatani, Akira Kawai, Hirokazu Chuman, Yasuo Beppu.
  • Journal Title: Jpn J Clin Oncol 37 Pages: 955-960
  • Peer Reviewed
• [Journal Article] Clear cell sarcoma of tendons and aponeuroses:A study of 75 patients. (2007)
  • Author(s): Akira Kawai, Ako Hosono, etc
  • Journal Title: Cancer 109 Pages: 109-116
  • Peer Reviewed
• [Journal Article] Dysadherin expression as a significant prognostic factor and as a determinant of histologic features in synovial sarcoma:Special reference to its inverse relationship with E-cadherin expression (2007)
  • Author(s): Teiyu Izumi, Akira Kawai, etc
  • Journal Title: Am J Surg Pathol 31 Pages: 85-94
  • Peer Reviewed
• [Journal Article] Clinical outcome of patients with Ewing sarcoma family of tumors of bone in Japan (2007)
  • Author(s): Hideto Obata, Akira Kawai, etc,
  • Journal Title: Cancer 109 Pages: 767-775
  • Peer Reviewed
• [Journal Article] Construct validity of the Enneking score for measuring function in patients with malignant or aggressive benign tumours of the upper limb (2007)
  • Author(s): Takuro Wada, Akira Kawai, etc
  • Journal Title: J Bone Joint Surg 89-B Pages: 659-663
  • Peer Reviewed
• [Journal Article] Gene expression analysis of soft tissue sarcomas:Characterization and reclassification of malignant fibrous histiocytoma (2007)
  • Author(s): Robert Nakayama, Akira Kawai, etc
  • Journal Title: Modern Pathology 20 Pages: 749-759
  • Peer Reviewed
• [Journal Article] New approach for assessing vascular distribution within bone tumors using dynamiccontrast-enhanced MRI (2007)
  • Author(s): Yukio Kawakami, Akira Kawai, etc
  • Journal Title: J Cancer Res Clin Oncol 133 Pages: 697-703
  • Peer Reviewed
• [Journal Article] Prognostic implications of glucose transporter protein-1(Glut-1)overexpression in bone and soft-tissue sarcomas (2007)
  • Author(s): Makoto Endo, Akira Kawai, etc
  • Journal Title: Jpn J Clin Oncol 37 Pages: 955-960
  • Peer Reviewed
• [Patent(Industrial Property Rights)] セセルニン-1の使用、滑膜肉腫の予後予測方法及び検査用試薬キット (2009)
  • Inventor(s): 近藤格、川井章、末原義之
  • Industrial Property Rights Holder: HS財団
  • Industrial Property Number: 2009-285045
  • Filing Date: 2009-12-16
  • Acquisition Date: 2009-12-16
• [Patent(Industrial Property Rights)] ペロキシレドキシン2の使用、骨肉腫の化学療法奏効性の予測方法及び検査試薬キット (2009)
  • Inventor(s): 近藤格、菊田一貴、川井章、廣橋説雄
  • Industrial Property Rights Holder: HS財団
  • Industrial Property Number: 2009-213605
  • Filing Date: 2009-09-15
  • Acquisition Date: 2009-09-15
• [Patent(Industrial Property Rights)] 消化管間質腫瘍(GIST)を処置するための医薬組成物、ならびに消化管間質腫瘍を患う患者の予後を予測するためのキットおよび方法 (2008)
  • Inventor(s): 近藤格、廣橋説雄、川井章、菊田一貴
  • Industrial Property Rights Holder: HS財団
  • Filing Date: 2008-11-27
  • Acquisition Date: 2009-11-27
• [Patent(Industrial Property Rights)] 消化管間質腫瘍(GIST)を処置するための医薬組成物、ならびに消化管間質腫瘍を患う患者の予後を予測するためのキットおよび方法 (2006)
  • Inventor(s): 近藤 格
  • Industrial Property Number: 2006-286087
  • Filing Date: 2006-10-20