The establishment of new cell therapy for bone regeneration by transplantation of culture expanded bone marrow cells and platelet rich plasma

• Project/Area Number: 19591755
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Nagoya University
• Principal Investigator: KITOH Hiroshi Nagoya University, 医学部附属病院, 講師 (40291174)
• Co-Investigator(Kenkyū-buntansha): KAWASUMI Motoaki 名古屋大学, 医学部附属病院, 医員 (00467309) ; 加藤 光康 名古屋大学, 医学部附属病院, 助教 (50378105)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 骨髄間葉系細胞 / 多血小板血漿 / 仮骨延長術 / 骨組織再生 / 細胞治療 / 骨細織再生 / 細胞培養

Research Abstract

培養骨髄細胞と多血小板血漿を用いた骨組織再生治療において、in vitroにおけるアルカリフォスファターゼ活性が高い骨髄細胞と、血小板濃度が高い多血小板血漿の組み合わせが最も骨再生に適していることを明らかにした。この結果に基づき、培養骨髄細胞と多血小板血漿を用いた仮骨延長術の臨床研究を継続し、大腿骨延長における細胞治療効果を確認した。また、その治療効果は移植細胞数および骨芽細胞への分化度に関連していたが、血小板数との関連は認めなかった。

Research Products

• [Journal Article] Differential effects of culture expanded bone marrow cells transplantation on the regeneration of bone between the femoral and the tibial lengthenings (2009)
  • Author(s): Kitoh H, Kawasumi M, Kaneko H, Ishiguro N
  • Journal Title: J Pediatr Orthop (accepted)
  • Peer Reviewed
• [Journal Article] Predictive factors for unsuccessful treatment of developmental dysplasia of the hip by the Pavlik harness (2009)
  • Author(s): Kitoh H, Kawasumi M, Ishiguro N
  • Journal Title: J Pediatr Orthop (accepted)
  • Peer Reviewed
• [Journal Article] Induction of systemic bone changes by preconditioning total body irradiation for bone marrow transplantation (2009)
  • Author(s): Miyazaki O, Nishimura G, Okamoto R, Masaki H, Kumagai M, Shioda Y, Nozawa K, Kitoh H
  • Journal Title: Pediatr Radiol 39 Pages: 23-29
  • Peer Reviewed
• [Journal Article] Differential effects of culture expanded bone marrow cells transplantation on the regeneration of bone between the femoral and the tibial lengthenings. (2009)
  • Author(s): Kitoh H, Kawasumi M, Kaneko H, Ishiguro N
  • Journal Title: J Pediatr Orthop accepted
  • Peer Reviewed
• [Journal Article] Predictive factors for unsuccessful treatment of developmental dysplasia of the hip by the Pavlik harness. (2009)
  • Author(s): Kitoh H, Kawasumi M, Ishiguro N
  • Journal Title: J Pediatr Orthop accepted
  • Peer Reviewed
• [Journal Article] Induction of systemic bone changes by preconditioning total body irradiation for bone marrow transplantation. (2009)
  • Author(s): Miyazaki O, Nishimura G, Okamoto R, Masaki H, Kumagai M, Shioda Y, Nozawa K, Kitoh H.
  • Journal Title: Pediatr Radiol 39 Pages: 23-29
  • Peer Reviewed
• [Journal Article] A case of mesomelic dyplasia Kantaputra type -new findings and a new diagnostic approach (2008)
  • Author(s): Siwicka KA, Kitoh H, Nishiyama M, Ishiguro N
  • Journal Title: J Pediatr Orthop (B) 17 Pages: 271-276
  • Peer Reviewed
• [Journal Article] The effect of the platelet concentration in platelet-rich plasma gel on the regeneration of bone (2008)
  • Author(s): Kawasumi M, Kitoh H, Siwicka KA, Ishigurio N
  • Journal Title: J Bone Joint Surg 90-B Pages: 966-972
  • Peer Reviewed
• [Journal Article] A histological and ultrastructural study of the iliac crest apophysis in Legg-Calve-Perthes disease (2008)
  • Author(s): Kitoh H, Kitakoji T, Kawasumi M, Ishiguro N
  • Journal Title: J Pediatr Orthop 28 Pages: 435-439
  • Peer Reviewed
• [Journal Article] Patellar dislocation in achondroplasia (2008)
  • Author(s): Takamine Y, Kitoh H, Ito H, Yazaki S, Oki T
  • Journal Title: J Pediatr Orthop (B) 17 Pages: 47-49
  • Peer Reviewed
• [Journal Article] A case of mesomelic dyplasia Kantaputra type-new findings and a new diagnostic approach. (2008)
  • Author(s): Siwicka KA, Kitoh H, Nishiyama M, Ishiguro N
  • Journal Title: J Pediatr Orthop (B) 17 Pages: 271-276
  • Peer Reviewed
• [Journal Article] The effect of the platelet concentration in platelet-rich plasma gel on the regeneration of bone. (2008)
  • Author(s): Kawasumi M, Kitoh H, Siwicka KA, Ishigurio N
  • Journal Title: J Bone Joint Surg 90-B Pages: 966-972
  • Peer Reviewed
• [Journal Article] A histological and ultrastructural study of the iliac crest apophysis in Legg-Calve-Perthes disease. (2008)
  • Author(s): Kitoh H, Kitakoji T, Kawasumi M, Ishiguro N
  • Journal Title: Pediatr Orthop 28 Pages: 435-439
  • Peer Reviewed
• [Journal Article] Patellar dislocation in achondroplasia. (2008)
  • Author(s): Takamine Y, Kitoh H, ItoH, Yazaki S, Oki T
  • Journal Title: J Pediatr Orthop (B) 17 Pages: 47-49
  • Peer Reviewed
• [Journal Article] Patellar dislocation in achondroplasia. (2008)
  • Author(s): Takamine Y, Kitoh H, Ito H, Yazaki S, Oki T.
  • Journal Title: J Pediatr Orthop(B) 17(1) Pages: 47-49
  • Peer Reviewed
• [Journal Article] A histological and ultrastructural study of the iliac crest apophysis in Legg-Calve-Perthes disease. (2008)
  • Author(s): Kitoh H, Kitakoji T, Kawasumi M. Ishiguro N.
  • Journal Title: J Pediatr Orthopn (accepted) (in press)
• [Journal Article] Acrodysostosis with unusual iridal color changing with age (2007)
  • Author(s): Nii E, Urawa M, Nishimura T, Kitoh H, Ikegawa S, Shimizu S, Taneda H, Uchida A, Niikawa N
  • Journal Title: Am J Med Genet 144B Pages: 824-825
  • Peer Reviewed
• [Journal Article] Sagittal spinal alignment in patients with Legg-Calve-Perthes disease (2007)
  • Author(s): Kitoh H, Kitakoji T, Katoh M, Ishiguro N
  • Journal Title: Pediatr Int 49 Pages: 612-617
  • NAID: 10020117985
  • Peer Reviewed
• [Journal Article] Distraction osteogenesis of the lower extremity in patients with achondrolpasia/hypochondroplasia treated with transplantation of culture expanded bone marrow cells and platelet rich plasma (2007)
  • Author(s): Kitoh H, Kitakoji T, Tsuchiya H, Katoh M, Ishiguro N
  • Journal Title: J Pediatr Orthop 27 Pages: 629-634
  • Peer Reviewed
• [Journal Article] The effect of recombinant human bone morphogenetic protein-2 on osteogenic potential of rat mesenchymal stem cells after several passages (2007)
  • Author(s): Ishikawa H, Kitoh H, Sugiura F, Ishiguro N
  • Journal Title: Acta Orthop 78 Pages: 285-292
  • Peer Reviewed
• [Journal Article] A recurrent mutation in type II collagen gene causes Legg-Calve-Perthes disease in a Japanese family (2007)
  • Author(s): Miyamoto Y, MatsudaT, Kitoh H, Haga N, Ohashi H, Nishimura G, Ikegawa S
  • Journal Title: Hum Genet 121 Pages: 625-629
  • Peer Reviewed
• [Journal Article] FBN2, FBN1, TGFBR1, and TGFBR2 analysis in congenital contractural arachnodactyly (2007)
  • Author(s): Nishimura A, Sakai H, Ikegawa S, Kitoh H, Haga N, Ishikiriyama S, Nagai T, Takeda F, Ohata T, Tanaka F, Kamasaki H, Saitsu H, Mizuguchi T, Matsumoto N
  • Journal Title: Am J Med Genet (A) 143 Pages: 694-698
  • Peer Reviewed
• [Journal Article] Transplantation of culture expanded bone marrow cells and platelet rich plasma in distraction osteogenesis of the long bones (2007)
  • Author(s): Kitoh H, Kitakoji T, Tsuchiya H, Katoh M, Ishiguro N
  • Journal Title: Bone 40 Pages: 522-528
  • Peer Reviewed
• [Journal Article] 軟骨細胞分化の分子機構 (2007)
  • Author(s): 鬼頭浩史, 石黒直樹
  • Journal Title: Clin Calcium 17(4) Pages: 493-498
  • Peer Reviewed
• [Journal Article] 骨の再生(長管骨) (2007)
  • Author(s): 鬼頭浩史, 石黒直樹
  • Journal Title: The Bone 21(4) Pages: 441-445
  • Peer Reviewed
• [Journal Article] Transplantation of culture expanded bone marrow cells and platelet rich plasma in distraction osteogenesis of the long. bones. (2007)
  • Author(s): Kitoh H, Kitakoji T, Tsuchiya H, Katoh M, Ishiguro N.
  • Journal Title: Bone 40 Pages: 522-528
  • Peer Reviewed
• [Journal Article] FBN2, FBN1, TGFBR1, and TGFBR2 analysis in congenital contractural arachnodactyly. (2007)
  • Author(s): Nishimura A, Sakai H, Ikegawa S, Kitoh H, (他10名)
  • Journal Title: Am J Med Genet (A) 143 Pages: 694-698
  • Peer Reviewed
• [Journal Article] A recurrent mutation in type II collagen gene causes Legg-Calve-Perthes disease in a Japanese family. (2007)
  • Author(s): Miyamoto Y, Matsuda T, Kitoh H, Haga N, (他2名)
  • Journal Title: Hum Genet 121 Pages: 625-629
  • Peer Reviewed
• [Journal Article] The effect of recombinant human bone morphogenetic protein-2 on osteogenic potential of rat mesenchymal stem cells after several passages. (2007)
  • Author(s): Ishikawa H, Kitoh H, Sugiura F, Ishiguro N.
  • Journal Title: Acta Orthop 78 Pages: 285-292
  • Peer Reviewed
• [Journal Article] Distraction osteogenesis of the lower extremity in patients with achondrolpasia/hypochondroplasia treated with transplantation of culture expanded bone marrow cells and platelet rich plasma (2007)
  • Author(s): Kitoh H, Kitakoji T, Tsuchiya H, Katoh M, Ishiguro N.
  • Journal Title: J Pediatr Orthop 27(6) Pages: 629-634
  • Peer Reviewed
• [Journal Article] Sagittal spinal alignment in patients with Legg-Calve-Perthes disease. (2007)
  • Author(s): Kitoh H, Kitakoji T, Katoh M, Nogami K, Ishiguro N.
  • Journal Title: Pediatr Int 49(5) Pages: 612-617
  • NAID: 10020117985
  • Peer Reviewed
• [Journal Article] Acrodysostosis with unusual iridal color changing with age. (2007)
  • Author(s): Nii E, Urawa M, Nishimura T, Kitoh H, Ikegawa S, (他4名)
  • Journal Title: Am J Med Genet (B) 144B Pages: 824-825
  • Peer Reviewed
• [Presentation] 錐体ジストロフィーを合併した脊椎骨幹端異形成症の1例 (2008)
  • Author(s): 川澄本明, 鬼頭浩史, 石黒直樹, 近藤峰生, 西村玄
  • Organizer: 第20回日本整形外科学会骨系統疾患研究会
  • Place of Presentation: 東京
  • Year and Date: 2008-12-12
• [Presentation] 先天性股関節脱臼に対するリーメンビューゲル法の治療成績 整復後の臼蓋形成に関連する因子の検討 (2008)
  • Author(s): 鬼頭浩史, 川澄本明, 石黒直樹
  • Organizer: 第19回日本小児整形外科学会
  • Place of Presentation: 東京
  • Year and Date: 2008-12-11
• [Presentation] 先天性股関節脱臼に対するリーメンビューゲル法の治療成績整復後の臼蓋形成に関連する因子の検討 (2008)
  • Author(s): 鬼頭浩史, 川澄本明, 石黒直樹
  • Organizer: 第19回日本小児整形外科学会
  • Place of Presentation: 東京
  • Year and Date: 2008-12-11
• [Presentation] 内旋歩行に対する固有受容器足底板の効果について (2008)
  • Author(s): 馬淵晃好, 鈴木伸治, 井上真郷, 鬼頭浩史, 川澄本明, 永谷元基, 林尊弘, 松本芳樹
  • Organizer: 第11回東海足と靴の研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-25
• [Presentation] 先天性股関節脱臼に対するリーメンビューゲル法の治療成績 (2008)
  • Author(s): 鬼頭浩史, 川澄本明、石黒直樹
  • Organizer: 第23回東海小児整形外科懇話会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-02-09
• [Presentation] Melnick-Needles骨異形成症の1例 (2008)
  • Author(s): 川澄本明, 鬼頭浩史, 吉橋裕治, 石黒直樹
  • Organizer: 第23回東海小児整形外科懇話会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-02-09
• [Presentation] Mesomelic dysplasia Kantaputra typeの1例 (2008)
  • Author(s): Karolina Anna Siwicka, 鬼頭浩史, 川澄本明, 西山正紀, 石黒直樹
  • Organizer: 第23回東海小児整形外科懇話会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-02-09
• [Presentation] ペルテス病における腸骨稜軟骨の組織形態学的検討 (2008)
  • Author(s): 鬼頭浩史, 川澄本明, 石黒直樹
  • Organizer: 第23回日本整形外科基礎学会
  • Place of Presentation: 東京
• [Presentation] 大腿骨頭すべり症に対する創外固定器を使用した矯正骨切り術の3例 (2008)
  • Author(s): 川澄本明, 鬼頭浩史, 加藤光康, 石黒直樹
  • Organizer: 第47回日本小児股関節研究会
  • Place of Presentation: 名古屋
• [Presentation] 先天性股関節脱臼に対するリーメンビューゲル法の治療成績に関連する因子の検討 (2008)
  • Author(s): 鬼頭浩史, 川澄本明, 石黒直樹
  • Organizer: 第47回日本小児股関節研究会
  • Place of Presentation: 名古屋
• [Presentation] 先天性内反足にPonseti法を施行した短期治療成績 (2008)
  • Author(s): 川澄本明, 鬼頭浩史, 石黒直樹, 北小路隆彦, 加藤光康
  • Organizer: 第81回日本整形外科学会
  • Place of Presentation: 札幌
• [Presentation] 培養骨髄細胞と多血小板血漿の移植を併用した下肢骨延長術 臨床成績に関連する因子の検討 (2008)
  • Author(s): 鬼頭浩史, 川澄本明, 石黒直樹
  • Organizer: 第81回日本整形外科学会
  • Place of Presentation: 札幌
• [Presentation] 培養骨髄細胞と多血小板血漿の移植を併用した下肢骨延長術の臨床成績 (2008)
  • Author(s): 鬼頭浩史, 川澄本明, 石黒直樹
  • Organizer: 第7回日本再生医療学会
  • Place of Presentation: 名古屋
• [Presentation] The effect of platelet concentration in platelet-rich plasma gel on bone regeneration (2008)
  • Author(s): Motoaki Kawasumi, Hiroshi Kitoh, Karolina A Siwicka, Naoki Ishiguro
  • Organizer: 53rd annual meeting of Orthopaedic Research Society
  • Place of Presentation: San Francisco
• [Presentation] Distraction osteogenesis of the lower limbs with transplantation of culture expanded bone marrow cells and platelet rich plasma (2008)
  • Author(s): Hiroshi Kitoh, Motoaki Kawasumi, Naoki Ishiguro
  • Organizer: 53rd annual meeting of Orthopaedic Research Society
  • Place of Presentation: San Francisco
• [Presentation] ペルテス病における腸骨稜軟骨の組織形態学的検討 (2008)
  • Author(s): 鬼頭浩史, 川澄本明, 石黒直樹
  • Organizer: 第23回日本整形外科基礎学会
  • Place of Presentation: 京都
• [Presentation] 培養骨髄細胞と多血小板血漿の移植を併用した下肢骨延長術臨床成績に関連する因子の検討 (2008)
  • Author(s): 鬼頭浩史, 川澄本明, 石黒直樹
  • Organizer: 第81回日本整形外科学会
  • Place of Presentation: 札幌
• [Presentation] 進行性骨化性線維異形成症における足部変形について (2007)
  • Author(s): 鬼頭浩史, 西田佳弘, 川澄本明, 石黒直樹
  • Organizer: 第11回東海足と靴の研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-10-20
• [Presentation] ペルテス病患児における腸骨稜軟骨の組織形態学的検討 (2007)
  • Author(s): 鬼頭浩史, 北小路隆彦, 加藤光康, 川澄本明、石黒直樹
  • Organizer: 第18回日本小児整形外科学会
  • Place of Presentation: 神戸
• [Presentation] 進行性骨化性線維異形成症(FOP)に関する臨床研究プロジェクト (2007)
  • Author(s): 芳賀信彦, 川端秀彦, 鬼頭浩史, 中島康晴
  • Organizer: 第18回日本小児整形外科学会
  • Place of Presentation: 神戸
• [Presentation] Transplantation of culture expanded bone marrow cells and platelet rich plasma during distraction osteogenesis (2007)
  • Author(s): Hiroshi Kitoh, Naoki Ishiguro
  • Organizer: clinical and experimental study 15th triennial congress of Asia Pacific Orthopaedic Association
  • Place of Presentation: Seoul
• [Presentation] Transplantation of culture expanded bone marrow cells and platelet rich plasma during distraction osteogenesis.-clinical and experimental study- (2007)
  • Author(s): Hiroshi Kitoh, Naoki Ishiguro.
  • Organizer: 15th triennial congress of Asia Pacific Orthopaedic Association
  • Place of Presentation: ソウル(韓国)
• [Book] Stickler症候群 最新整形外科体系 骨系統疾患、代謝性骨疾患 (2007)
  • Author(s): 鬼頭浩史
  • Publisher: 中山書店
• [Book] 先端異骨症 最新整形外科体系 骨系統疾患、代謝性骨疾患 (2007)
  • Author(s): 北小路隆彦, 鬼頭浩史
  • Publisher: 中山書店
• [Book] 遅発性脊椎骨端異形成症 最新整形外科体系 骨系統疾患、代謝性骨疾患 (2007)
  • Author(s): 鬼頭浩史
  • Publisher: 中山書店
• [Book] 偽性軟骨無形成症 最新整形外科体系 骨系統疾患、代謝性骨疾患 (2007)
  • Author(s): 鬼頭浩史
  • Publisher: 中山書店
• [Book] 軟骨無形成症 骨系統疾患マニュアル2版 (2007)
  • Author(s): 鬼頭浩史
  • Publisher: 南江堂
• [Book] 軟骨低形成症 骨系統疾患マニュアル2版 (2007)
  • Author(s): 鬼頭浩史
  • Publisher: 南江堂
• [Book] 屈曲肢異形成症 骨系統疾患マニュアル 2版 (2007)
  • Author(s): 鬼頭浩史
  • Publisher: 南江堂