| Project/Area Number |
19591770
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kinki University |
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Principal Investigator |
FUKUDA Kanji Kinki University, 医学部, 教授 (50201744)
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| Co-Investigator(Kenkyū-buntansha) |
OTANI Kazuhiro 近畿大学, 医学部, 講師 (20258031)
NONAKA Togo 近畿大学, 医学部, 講師 (70268407)
ASADA Shigeki 近畿大学, 医学部, 講師 (00330283)
NISHISAKA Fumiaki 近畿大学, 医学部, 講師 (80330314)
TERAMURA Takeshi 近畿大学, 医学部附属病院, 助教 (40460901)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2009: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2008: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2007: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
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| Keywords | 軟骨 / ストレス / 活性酸素 / cartilage / chondrocyte / mechanical stress / regenerative medicine / reactive oxygen species / hyaluronan / mechanicalstress / tissue engineering / regemeratove medocine |
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| Research Abstract |
Introduction : To repair the articular cartilage defect, cartilage explant is useful method. The key leading to the success of this regenerative medicine is to regulate the mechanical stress loaded on articular cartilage explant. Intraarticular hyaluronan (HA) injection is an effective treatment for cartilage degeneration. The purpose of this study is to determine the effect of mechanical stress loaded on the cartilage explant and possible usefulness of HA in this system.
Methods : Mechanical compression was loaded on the bovine cartilage using the Biopress system. Proteoglycan (PG) and reactive oxygen species (ROS) synthesis were measured with [^<35>S] incorporation and fluorescent dye, respectively. Accumulation of peroxynitrite was determined with western blotting using nitrotyrosine antibody.
Results : Mechanical compression inhibited PG synthesis and enhanced ROS. Externally added HA reversed stress-inhibited PG synthesis and attenuated ROS synthesis. HA also significantly decreased the expression of nitrotyrosine.
Conclusions : Mechanical stress is essential factor to degradate the explanted cartilage and ROS play an important role. HA neutralized stress-enhanced ROS synthesis and resulted in the reverse of PG synthesis. These data suggest that HA plays anabolic effect as an antioxidant.
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