Visible evaluation and pathologic clarification for response at central nervous system and peripheral tissue in arthritis of transgenic animal.

• Project/Area Number: 19591771
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: OHNISHI Hideo University of Occupational and Environmental Health, Japan, 医学部, 講師 (20279342)
• Co-Investigator(Kenkyū-buntansha): 中村 利孝 産業医科大学, 医学部, 教授 (50082235) ; 上田 陽一 産業医科大学, 医学部, 教授 (10232745) ; 田中 伸哉 産業医科大学, 医学部, 助教 (20412645)
• Co-Investigator(Renkei-kenkyūsha): NAKAMURA Toshitaka 産業医科大学, 医学部, 教授 (50082235) ; UETA Yoichi 産業医科大学, 医学部, 教授 (10232745) ; TANAKA Shinya 産業医科大学, 医学部, 助教 (20412645)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 関節炎 / 遺伝子改変動物 / バゾプレッシン / ストレス / 緑色蛍光タンパク

Research Abstract

関節リウマチの動物モデルラットでは、体液を保持する働きがあるホルモンのバソプレシンと新陳代謝の制御や免疫反応に関わるホルモンのコルチコステロンの血液中濃度が上昇した。この機序として、下垂体後葉から分泌されるバソプレシンが、副腎皮質刺激ホルモンの分泌刺激を通してコルチコステロン濃度を上昇させることを、視床下部バソプレシン分泌神経細胞と神経線維が緑色に光る遺伝子を導入したラットを用いて明らかにした。

Research Products

• [Journal Article] Response of arginine vasopressin-enhanced green fluorescent protein fusion gene in the hypothalamus of adjuvant-induced arthritic rats. (2009)
  • Author(s): Suzuki, H. Onaka, T. Kasai, M. Kawasaki, M. Ohnishi, H. Otsubo, H. Saito, T. Hashimoto,H. Yokoyama, T. Fujihara, H. Dayanithi, G. Murphy, D. Nakamura, T. Ueta Y.
  • Journal Title: Journal of Neuroendocrinology 21(3) Pages: 183-190
  • Peer Reviewed
• [Journal Article] Response of arginine vasopressin-enhanced green fluorescent protein fusion gene in the hypothalamus of adjuvant-induced arthritic rats (2009)
  • Author(s): Suzuki, H., Onaka, T., Kasai M., Kawasaki, M., Ohnishi, H., Otsubo, H., Saito, T., Hashimoto, H., Yokoyama, T., Fujihara, H., Dayanithi, G., Murphy, D., Nakamura, T., Ueta, Y.
  • Journal Title: Journal of Neuroendocrinology 21(3) Pages: 183-190
  • Peer Reviewed
• [Journal Article] Adrenomedullin 2 / intermedin-like immunoreactivity in the hypothalamus and brainstem of rats. (2008)
  • Author(s): Hashimoto, H. Kitamura, K. Kawasaki, M. Saito, T. Suzuki, H. Otsubo, H. Ohbuchi, T. Yokoyama, T. Fujihara, H. Takei, Y. Ueta, Y.
  • Journal Title: Autonomic Neuroscience : Basic and Clinical 139(1-2) Pages: 46-54
  • Peer Reviewed
• [Journal Article] Distribution of orexins-containing fibers and contents of orexins in the rat olfactory bulb. (2008)
  • Author(s): Shibata, M. Mondal, MS. Date, Y. Nakazato, M. Suzuki, H. Ueta, Y.
  • Journal Title: Neuroscience Research 61(1) Pages: 99-105
  • Peer Reviewed
• [Journal Article] Adrenomedullin 2/intermedin-like immunoreactivity in the hypothalamus and brainstem of rats. (2008)
  • Author(s): Hashimoto, H., Kitamura, K., Kawasaki, M., Saito, T., Suzuki, H., Otsubo, H., Ohbuchi, T., Yokoyama, T., Fujihara, H., Takei, Y., Ueta, Y.
  • Journal Title: Autonomic Neuroscience : Basic and Clinical 139(1-2) Pages: 46-54
  • Peer Reviewed
• [Journal Article] Distribution of orexins-containing fibers and contents of orexins in the rat olfactory bulb (2008)
  • Author(s): Shibata, M., Mondal, MS., Date, Y., Nakazato, M., Suzuki, H., Ueta, Y.
  • Journal Title: Neuroscience Research 61(1) Pages: 99-105
  • Peer Reviewed
• [Journal Article] Centrally and peripherally administered ghrelin potently inhibits water intake in rats. (2007)
  • Author(s): Hashimoto, H. Fujihara, H. Kawasaki, M. Saito, T. Shibata, M. Otsubo, H. Takei, Y. Ueta, Y.
  • Journal Title: Endocrinology 148(4) Pages: 1638-1647
  • Peer Reviewed
• [Journal Article] Adrenomedullin 2 (AM2)/intermedin is a more potent activator of hypothalamic oxytocin-secreting neurons than AM possibly through an unidentified receptor in rats. (2007)
  • Author(s): Hashimoto, H. Hyodo, S. Kawasaki, M. Shibata, M. Saito, T. Suzuki, H. Otsubo, H. Yokoyama, T. Fujihara, H. Higuchi, T. Takei, Y. Ueta, Y.
  • Journal Title: Peptides 28(5) Pages: 1104-1112
  • Peer Reviewed
• [Journal Article] Downregulation of prolactin-releasing peptide gene expression in the hypothalamus and brainstem of diabetic rats. (2007)
  • Author(s): Mera, T. Fujihara, H. Saito, J. Kawasaki, M. Hashimoto, H. Saito, T. Shibata, M. Onaka, T. Tanaka, Y. Oka, T. Tsuji, S. Ueta, Y.
  • Journal Title: Peptides 28(8) Pages: 1596-1604
  • Peer Reviewed
• [Journal Article] Centrally and peripherally administered ghrelin potently inhibits water intake in rats.Endocrinology (2007)
  • Author(s): Hashimoto, H. Fujihara, H. Kawasaki, M. Saito, T. Shibata, M. Otsubo, H. Takei, Y.& Ueta, Y
  • Journal Title: Endocrinology 148(4) Pages: 1638-1647
  • Peer Reviewed
• [Journal Article] Adrenomedullin 2(AM2)/intermedin is a more potent activator of hypothalamic oxytocin-secreting neurons than AM possibly through an unidentified receptor in rats (2007)
  • Author(s): Hashimoto, H. Hyodo, S. Kawasaki, M. Shibata, M. Saito, T. Suzuki, H. Otsubo, H. Yokoyama, T. Fujihara, H. Higuohi, T. Takei, Y.& Ueta, Y
  • Journal Title: Peptides 28(5) Pages: 1104-1112
  • Peer Reviewed
• [Journal Article] Downregulation of prolactin-releasing peptide gene expression in the hypothalamus and brainstem of diabetic rats (2007)
  • Author(s): Mera, T. Fujihara, H. Saito, J. Kawasaki, M. Hashimoto, H. Saito, T. Shibata, M. Onaka, T. Tanaka, Y. Y.Oka, T.Tsuji, S.Ueta, Y.
  • Journal Title: Peptides 28(8) Pages: 1596-1604
  • Peer Reviewed
• [Presentation] The expression of the hypothalamic feeding-regulating peptides genes in adjuvant-induced arthritic rats. (2008)
  • Author(s): Suzuki, H. Kasai, M. Kawasaki, M. Ohnishi, H. Otsubo, H. Saito, T. Hashimoto, H. Yokoyama, T. Fujihara, H. Nakamura, T. Ueta, Y.
  • Organizer: 9th International NPY Meeting
  • Place of Presentation: Okinawa, Japan
• [Presentation] Response of arginine vasopressin-enhanced green fluorescent protein fusion gene in the hypothalamus of formalin-induced nociceptive stimulated rats. (2008)
  • Author(s): Suzuki, H. Kawasaki, M. Ohnishi, H. Otsubo, H. Yokoyama, T. Fujihara, H. Dayanithi, G. Murphy, D. Nakamura, T. Ueta, Y.
  • Organizer: The 3rd Asian Pain Symposium
  • Place of Presentation: Fukuoka, Japan
• [Presentation] Effects of formalin-induced nociceptive stimulation in the hypothalamus of arginine vasopressin-enhanced green fluorescent protein transgenic rats. (2008)
  • Author(s): Suzuki, H. Kawasaki, M. Ohnishi, H. Otsubo, H. Ohbuchi, T. Hashimoto, H. Yokoyama, T. Fujihara, H. Dayanithi, G. Murphy, D. Nakamura, T. Ueta, Y.
  • Organizer: Society for Neuroscience Annual Meeting
  • Place of Presentation: Washington DC, USA
• [Presentation] 関節炎モデルラットならびに癌悪液質モデルラットにおける視床下部摂食関連ペプチド遺伝子発現 (2008)
  • Author(s): 鈴木仁士, 上田陽一, 渡部美穂, 小沼悦郎, 岡部尚文, 橋本弘史, 藤原広明, 多久和陽, 尾形悦郎
  • Organizer: 第81回日本内分泌学会学術総会
  • Place of Presentation: 青森
• [Presentation] Response of arginine vasopressin-enhanced green fluorescent protein fusion gene in the hypothalamus of formalin-induced nociceptive stimulated rats (2008)
  • Author(s): Suzuki, H., Kawasaki, M., Ohnishi, H., Otsubo, H., Yokoyama, T., Fujih H., Otsubo, H., Yokoyama, T., Fujiara, H., Dayanithi, G., Murphy, D., Nakamura, T., Ueta, Y.
  • Organizer: The 3^<rd> Asian Pain Symposium
  • Place of Presentation: Fukuoka, Japan
• [Presentation] Effects of formalin-induced nociceptive stimulation in the hypothalamus of arginine vasopressin-enhanced green fluorescent protein transgenic rats (2008)
  • Author(s): Suzuki, H., Kawasaki, M., Ohnishi, H., Otsubo, H., Ohbuchi, T., Hashimoto, H., Yokoama, T., Fujihara, H., Dayanithi, G., Murphy, D., Nakamura, T., Ueta, Y.
  • Organizer: Society for Neuroscience Annual Meeting
  • Place of Presentation: Washington DC, USA
• [Presentation] アジュバント関節炎におけるバゾプレッシン(AVP)の役割 : AVP-eGFPトランスジェニックラットを用いた検討 (2007)
  • Author(s): 鈴木仁士、尾仲達史、笠井聖仙、川崎展、大西英生、大坪広樹、藤原広明、Govindan Dayanithi、David Murphy、中村利孝、上田陽一
  • Organizer: 第7回日本内分泌学会九州地方会
  • Place of Presentation: 福岡
  • Year and Date: 2007-09-01
• [Presentation] アジュバント関節炎におけるバゾプレッシン(AVP)の役割:AVP-eGFPトランスジェニックラットを用いた検討 (2007)
  • Author(s): 鈴木 仁士、尾仲 達史、笠井 聖仙、川崎 展、大西 英生、大坪 広樹、藤原 広明、Govindan Dayanithi、David Murphy、中村 利孝、上田 陽一
  • Organizer: 第7回日本内分泌学会九州地方会
  • Place of Presentation: 福岡
  • Year and Date: 2007-09-01
• [Presentation] ラットの中枢および末梢に投与したグレリンの飲水への影響 (2007)
  • Author(s): 橋本弘史、藤原広明、川崎展、柴田美雅、齋藤健、大坪広樹、竹井祥郎、上田陽一
  • Organizer: 第3回環境生理学プレコングレス
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-19
• [Presentation] Response of arginine-enhanced green fluorescent protein fusion gene in the hypothalamus of adjuvant-induced arthritic rats. (2007)
  • Author(s): Suzuki, H. Onaka, T. Kasai, M. Kawasaki, M. Ohnishi, H. Otsubo, H. Fujihara, H. Nakamura, T. Dayanithi, G. Murphy, D. Ueta, Y.
  • Organizer: Society for Neuroscience 36th Annual Meeting
  • Place of Presentation: San Diego, California
• [Presentation] Salusin-b evoked the neurohypophyseal hormone release from the neurohypophysis. (2007)
  • Author(s): Saito, T. Dayanithi, G. Watanabe, T.X. Urabe, T. Hashimoto, H. Suzuki, H. Yokoyama, T. Fujihara, H. Onaka, T. Hirata, Y. Ueta, Y.
  • Organizer: Society for Neuroscience 36th Annual Meeting
  • Place of Presentation: San Diego, California
• [Presentation] バゾプレッシン(AVP)-eGFPトランスジェニックラットを用いたアジュバント関節炎におけるAVPの役割の検討 (2007)
  • Author(s): 鈴木仁士、尾仲達史、笠井聖仙、川崎展、大西英生、藤原広明、Govindan Dayanithi、David Murphy、中村利孝、上田陽一
  • Organizer: 第58回西日本生理学会
  • Place of Presentation: 福岡
• [Presentation] 急性浸透圧刺激時のラット下垂体後葉におけるガラニン様ペプチド遺伝子発現 (2007)
  • Author(s): 川崎展、斎藤淳、橋本弘史、鈴木仁士、大坪広樹、藤原広明、上田陽一
  • Organizer: 第84回日本生理学会
  • Place of Presentation: 大阪
• [Presentation] アドレノメデュリン2の神経内分泌系への作用のアドレノメデュリンとの比較 (2007)
  • Author(s): 橋本弘史、兵藤晋、川崎展、斎藤健、大坪広樹、藤原広明、樋口隆、竹井祥郎、上田陽一
  • Organizer: 第84回日本生理学会
  • Place of Presentation: 大阪
• [Presentation] Response of arginine-enhanced green fluorescent protein fusion gene in the hypothalamus of adjuvant-induced amhritio rats (2007)
  • Author(s): Suzuki, H.Onaka, T.Kasai, M.Kawasaki, M, Ohnishi, H.Otsubo, H.Fujihara, H.Nakamura, T.Dayanithi, G.Murphy, D.& UetaY
  • Organizer: Society for Neuroscience 36th Annual Meeting
  • Place of Presentation: San Diego,Califbrnia
• [Presentation] Salusin-β evoked the neurohypophyseal hormone release from the neurohypophysis (2007)
  • Author(s): Saito, T.Dayanithi, G.Watanabe, T.X.Urabe, T.Hashimoto, H.Suzuki, H.Yokoyama, T.Fujihara, H.Onaka, T.Hirata, Y & Ueta, Y.
  • Organizer: Society for Neuroscience 36th Annual Meeting
  • Place of Presentation: San Diego,California