The searching a mechanism of general anesthesia modulated by non-synaptic communication of nitric oxide in the brain

• Project/Area Number: 19591787
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: ADACHI Yushi Hamamatsu University School of Medicine, 医学部附属病院, 助教 (80420355)
• Co-Investigator(Kenkyū-buntansha): SATO Shigehito 浜松医科大学, 医学部, 教授 (30143176)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 麻酔学 / 一酸化窒素 / 麻酔薬 / ペントバルビタール / ケタミン / マイクロダイアリシス法 / ニトログリセリン / ニトロプルシド

Research Abstract

全身麻酔は日常臨床の場において広く用いられているにも関わらず、その麻酔作用発揮のメカニズムは未知のままである。我々は、近年注目されている神経伝達物質である一酸化窒素と、その非シナプス型神経伝達に注目し、麻酔薬の作用機序解明を目指した。これまでに、麻酔薬が非シナプス型神経伝達をも抑制し、他の神経伝達物質の制御にも関わっている事を発見した。

Research Products

• [Journal Article] 佐藤重仁アミトリプチリンとパロキセチンでともにセロトニン症候群が生じたと考えられた1例 (2009)
  • Author(s): 足立裕史, 山口昌一, 中島芳樹, 五十嵐寛
  • Journal Title: 日本ペインクリニック学会誌 16 Pages: 23-25
  • Peer Reviewed
• [Journal Article] Isoflurane anesthesia inhibits clozapine-and risperidone-induced dopamine release and anesthesia-induced changes in dopamine metabolism was modified by fluoxetine in the rat striatum: an in vivo microdialysis study. (2009)
  • Author(s): Adachi YU, Yamada S, Satomoto M, Higuchi H, Watanabe K, Kazama T, Mimuro S, Sato S.
  • Journal Title: Eur J Anaesthesiol 26,45 Pages: 100-1
  • Peer Reviewed
• [Journal Article] Nafamostat prevents hypothermia and improves survival time after administration of lipopolysaccharide in a mouse surgical model (2009)
  • Author(s): Adachi YU, Obata Y, Suzuki K, Katoh H, Itagaki T, Doi M, Sato S.
  • Journal Title: J Anesth 23 Pages: 624-7
  • NAID: 10027265316
  • Peer Reviewed
• [Journal Article] Nafamostat prevents hypothermia and improves survival time after administration of lipopolysaccharide. (2008)
  • Author(s): Adachi YU, Obata Y, Suzuki K, Katoh H, ItagakiT, Doi M, Sato S.
  • Journal Title: J Anesth 23 Pages: 624-7
• [Journal Article] Pentobarbital-induced reduction of nitric oxide in the rat brain striatum was antagonized by nicotine and NMDA. (2008)
  • Author(s): Adachi YU, Itagaki T, Obata Y, Shiraishi Y, Sato S.
  • Journal Title: Eur J Anaesthesiol 25,44 Pages: 129-30
  • Peer Reviewed
• [Journal Article] Propofol-induced reduction of nitric oxide in rat brain was antagonized by neostigmine and arginine. (2008)
  • Author(s): Adachi YU, Kimura K, Mimuro S, Doi M, Sato S.
  • Journal Title: Anesthesiology 109
  • Peer Reviewed
• [Journal Article] Isofurane anesthesia inhibits clozapine-and risperidone・induced dopamine release and anesthesia-induced changes in dopamine metabolism was modified by fluoxetine in the rat striatum:an in vivo microdialysis study (2008)
  • Author(s): Adachi YU, Yamada S, Satomoto M, et. al.
  • Journal Title: Neurochemistry International 52 Pages: 384-91
  • Peer Reviewed
• [Journal Article] Ketamine Increased Nitric Oxide Release in the Rat Striatum Independent from Acetylcholine Release. (2007)
  • Author(s): Adachi YU, Mimuro S, Uraoka M, Shiraishi Y, Sato S.
  • Journal Title: Anesthesiology 107
  • Peer Reviewed
• [Journal Article] Pentobarbital inhibits the release of nitric oxide and the effect is antagonized by the application of neostigmine and magnesium-free perfusion in the rat striatum: an in vivo microdialysis study. (2007)
  • Author(s): Adachi YU, Mimuro S, Doi M, Sato S.
  • Journal Title: Eur J Anaesthesiol 24,39 Pages: 82-3
  • Peer Reviewed
• [Presentation] The nitroglycerine-induced nitric oxide release was enhanced by propoofl in rats striatum-in vivo microdialysis study (2009)
  • Author(s): Adachi YU, Kimura K, Mimuro S, Doi M, Sato S.
  • Organizer: Eur J Anaesthesiol 2009 ; 26 Supple 45:100 (7AP3-6)
  • Place of Presentation: Milan, Italy
• [Presentation] Adachi YU, Kimura K, Mimuro S, Doi M, Sato S. The release of nitricoxide was anhanced by GABAergic inhibition and NMDAergic potentiation and the both effects were antagonized by propofol and sevoflurane anesthesia in rats striatum (2009)
  • Author(s): Adachi YU, Kimura K, Mimuro S, Doi M, Sato S.
  • Organizer: Eur J Anaesthesiol 2009 ; 26 Supple 45:100 (7AP3-6)
  • Place of Presentation: Milan, Italy
• [Presentation] The Nitroglycerine-Induced Nitric Oxide Release Was Enhanced by Propofol Not by Sevoflurane in Rats (2009)
  • Author(s): Adachi YU, Kimura K, Mimuro S, Itagaki T, Sato S.
  • Organizer: American society of Anesthesiologists, Annual Meeting 2009
  • Place of Presentation: New Orleans, LA, USA
• [Presentation] Propofol-induced reduction of nitric oxide in rat brain was antagonized by neostigmine and arginine. (2008)
  • Author(s): Adachi YU, Kimura K, Mimuro S, Doi M, Sato S.
  • Organizer: American Society of Anesthesiologists, Annual Meeting
  • Place of Presentation: Orlando Florida
  • Year and Date: 2008-10-21
• [Presentation] ぺンドバルビタールによるラツト脳線条体-酸化窒素放出抑制作用はニコンおよびNMDAによって拮抗される (2008)
  • Author(s): 足立裕史
  • Organizer: 目本麻酔科学会第55回学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-12
• [Presentation] Pentobarbital-induced reduction of nitric oxide in the rat brain striatum was antagonized by nicotine and NMDA. (2008)
  • Author(s): Adachi YU, Itagaki T, Obata Y, Shiraishi Y, Sato S.
  • Organizer: Euroanaesthesia 2008, The European Anaesthesiology Congress
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2008-06-01
• [Presentation] Sevoflurane-induced reduction of nitric oxide in the rat brain striatum, is antagonized by nicotine and neostigmine, but not by NMDA. (2008)
  • Author(s): Itagaki T, Obata Y, Adachi YU, Shiraishi Y, Sato S.
  • Organizer: Euroanaesthesia 2008, The European Anaesthesiology Congress
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2008-06-01
• [Presentation] Ketamine Increased Nitric Oxide Release in the Rat Striatum Independent from Acetylcholine Release. (2007)
  • Author(s): Adachi YU, et. al.
  • Organizer: American Society of Anesthesiologists, Annual Meeting
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2007-10-13
• [Book] 土井松幸訳稲田英一監訳精神機能の異常in ICUブック(第3版メディカル・サイエンス・インターナショナル東京) (2008)
  • Author(s): 足立裕史