Novel approach with sonoporation in IL12 gene therapy for prostate cancer.

• Project/Area Number: 19591851
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Okayama University
• Principal Investigator: SAIKA Takashi Okayama University, 大学院・医歯薬学総合研究科, 准教授 (10314676)
• Co-Investigator(Kenkyū-buntansha): NASU Yasutomo 岡山大学, 岡山大学病院, 教授 (20237572) ; KOBUKE Makoto 岡山大学, 岡山大学病院, 助教 (50452579) ; EDAMURA Kohei 岡山大学, 岡山大学病院, 医員 (90535816)
• Research Collaborator: EBARA Shin 岡山大学, 医学部・歯学部附属病院, 助教 (70379741)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 前立腺癌 / 遺伝子治療 / 高密度焦点超音波 / 遺伝子導入 / インターロイキン12 / 高密度焦点超音波(HIFU) / 非ウイルスベクター / IL-12

Research Abstract

To examine the efficacy and to establish of high intensity focused ultrasound (HIFU)-mediated gene therapy in the treatment of prostate cancer, we performed Interferon-12 (IL-12) gene therapy combined with sonoporation, using preclinical model of orthotopic murine prostate cancer.

Research Products

• [Journal Article] Potent antitumor effects of combined therapy with a telomerase-specific (2010)
  • Author(s): Huang P, Kaku H, Chen J, Kashiwakura Y, Saika T, Nasu Y, Urata Y, Fujiwara T, Watanabe M, Kumon H
  • Journal Title: replication-competent adenovirus (OBP-301) and IL-2 in a mouse model of renal cell carcinoma. Cancer Gene Ther
• [Journal Article] A comparison of proteomic profiles changes during 17beta-estradiol treatment in human prostate cancer PC-3 cell line. (2009)
  • Author(s): Chen J, Huang P, Kaku H, Zhang K, Watanabe M, Saika T, Nasu Y, Kumon H
  • Journal Title: Cancer Genomics Proteomics 6 Pages: 331-5
• [Journal Article] Prospective longitudinal comparative study of health-related quality of life in patients treated with radical prostatectomy or permanent brachytherapy for prostate cancer. (2009)
  • Author(s): Kobuke M, Saika T, Nakanishi Y, Ebara S, Manabe D, Uesugi T, Nose H, Arata R, Tsushima T, Nasu Y, Kumon H
  • Journal Title: Acta Med Okayama 63(3) Pages: 129-35
  • NAID: 120002313130
• [Journal Article] Initial report of hybrid radical prostatectomy for prostate cancer: reduced bleeding, clear vision, and secure surgical margins. (2008)
  • Author(s): Saika T, Kobayashi Y, Watanabe T, Manabe D, Ebara S, Uehara S, Nasu Y, Kumon H
  • Journal Title: Acta Med Okayama 62(6) Pages: 379-84
• [Journal Article] Identification of the HERV-K gag antigen in prostate cancer by SEREX using autologous patient serum and its immunogenicity. (2008)
  • Author(s): Ishida T, Obata Y, Ohara N, Matsushita H, Sato S, Uenaka A, Saika T, Miyamura T, Chayama K, Nakamura Y, Wada H, Yamashita T, Morishima T, Old LJ, Nakayama E
  • Journal Title: Cancer Immun 8 Pages: 15-15
• [Journal Article] Iodine-125 seed impla(permanent brachytherapy) for clinically localized prostate cancer. (2008)
  • Author(s): Ebara S, Katayama N, Tanimoto R, Edamura K, Nose H, Manabe D, Kobayashi T, Kobayashi Y, Kobuke M, Takemoto M, Saika T, Nasu Y, Kanazawa S, Kumon H
  • Journal Title: Acta Med Okayama 62(1) Pages: 9-13
• [Journal Article] Direct and distant antitumor effects of a telomerase-selective oncolytic adenoviral agent, OBP-301, in a mouse prostate cancer model. (2008)
  • Author(s): Huang P, Watanabe M, Kaku H, Kashiwakura Y, Chen J, Saika T, Nasu Y, Fujiwara T, Urata Y, Kumon H
  • Journal Title: Cancer Gene Ther 15(5) Pages: 315-22
• [Journal Article] Adenovirus-mediated REIC/Dkk-3 gene transfer inhibits tumor growth and metastasis in an orthotopic prostate cancer model. (2007)
  • Author(s): Edamura K, Nasu Y, Takaishi M, Kobayashi T, Abarzua F, Sakaguchi M, Kashiwakura Y, Ebara S, Saika T, Watanabe M, Huh NH, Kumon H
  • Journal Title: Cancer Gene Ther 14(9) Pages: 765-72
• [Journal Article] Heat shock proteins play a crucial role in tumor-specific apoptosis by REIC/Dkk-3. (2007)
  • Author(s): Abarzua F, Sakaguchi M, Tanimoto R, Sonegawa H, Li DW, Edamura K, Kobayashi T, Watanabe M, Kashiwakura Y, Kaku H, Saika T, Nakamura K, Nasu Y, Kumon H, Huh NH
  • Journal Title: Int J Mol Med 20(1) Pages: 37-43
• [Journal Article] T cell immunomonitoring and tumor responses in patients immunized with a complex of cholesterol-bearing hydrophobized pullulan (CHP) and NY-ESO-1 protein. (2007)
  • Author(s): Uenaka A, Wada H, Isobe M, Saika T, Tsuji K, Sato E, Sato S, Noguchi Y, Kawabata R, Yasuda T, Doki Y, Kumon H, Iwatsuki K, Shiku H, Monden M, Jungbluth AA, Ritter G, Murphy R, Hoffman E, Old LJ, Nakayama E
  • Journal Title: Cancer Immun 7 Pages: 9-9
• [Journal Article] Suicide gene therapy with adenoviral delivery of HSV-tK gene for patients with local recurrence of prostate cancer after hormonal therapy. (2007)
  • Author(s): Nasu Y, Saika T, Ebara S, Kusaka N, Kaku H, Abarzua F, Manabe D, Thompson TC, Kumon H
  • Journal Title: Mol Ther 15(4) Pages: 834-40