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The correlation between bladder outlet obstruction and calcium sensitization mechanism in human and guinea pig detrusor smooth muscle

Research Project

Project/Area Number 19591857
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionKyushu University

Principal Investigator

SEKI Narihito  Kyushu University, 大学院医学研究院, 准教授 (90294941)

Co-Investigator(Renkei-kenkyūsha) KAJIOKA Syunichi  九州大学, 大学院・医学研究院, 特任講師 (90274472)
TAKAHASHI Ryousuke  九州大学, 大学病院, 医員 (30529805)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords排尿筋 / 膀胱出口閉塞 / 過活動膀胱 / Ca^<2+>感受性調節機序 / Rho kinase / モルモット / 細胞内Ca^<2+>濃度 / スキンド標本 / RhoA-kinase / 下部尿路機能障害 / カルバコール / αトキシン / カルシウム感受性 / ムスカリン受容体
Research Abstract

モルモットにおいて、膀胱出口閉塞((Bladder outlet obstruction : BOO)作成群ではSHAM手術群と比較してRho kinase阻害薬であるY-27632投与(5μM)により有意にカルバコールにより発生する収縮力が減少した。この結果はBOO に伴い細胞内 Ca_<2+>濃度([Ca_<2>+]_i)非依存性収縮ひいてはRho kinaseカスケードが増強されることを示唆した。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (20 results)

All 2009 2008 2007 Other

All Journal Article (10 results) (of which Peer Reviewed: 9 results) Presentation (10 results)

  • [Journal Article] Photoselective vaporization of the prostate : Outcome according to the prostate size in a series of 102 Japanese patients2009

    • Author(s)
      Nomura H, Seki N, Yamaguchi A, Naito S
    • Journal Title

      Int J Urol 16

      Pages: 87-90

    • NAID

      10025195724

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of photoselectivevaporization of the prostate(PVP)onurodynamics in patients with benignprostatic enlargement2009

    • Author(s)
      Seki N, Nomura H, Yamaguchi A, NaitoS
    • Journal Title

      J Urol 180

      Pages: 1024-1029

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Levcromakalim and MgGDP activate small conductance ATP-sensitive K channels of K channel pore 6.1/sulfonylurea receptor 2A in pig detrusor smooth muscle cells2008

    • Author(s)
      Kajioka S, Nakayama S, Asano N, Seki N, Naito s, Brading AF
    • Journal Title

      Uncoupling of cAMP signal pathways.J Pharmacol Exp Ther 327(1)

      Pages: 114-123

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Oscillatory membranecurrents paradoxically induced viaNO-activated pathways in pig detrusorcells2008

    • Author(s)
      Kajioka S, Nakayama S, Seki N, Naito s, Brading AF
    • Journal Title

      Cell Carcium 44

      Pages: 202-209

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of flavoxate hydrochloride on voltage-dependent Ba_<2+> currents inhuman detrusor myocytes at different experimental temperatures2008

    • Author(s)
      Tomoda T, Zhu HL, Iwasa K, Aishima M, Shibata A, Seki N, Naito S, Teramato N
    • Journal Title

      Naunyn-Schmiedebergs Arch Pharmacol 376(3)

      Pages: 195-203

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Oscillatory membrane currents paradoxically induced via NO-activated pathways in pig detrusor cells2008

    • Author(s)
      Kajioka S, Nakayama S, Seki N, et al
    • Journal Title

      Cell Calcium 44

      Pages: 202-209

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Levcromakalim and MgGDP activate small conductance ATP-sensitive channels of K channel pore 6.1/sulfo-nylurea receptor 2A in pig detrusor smooth muscle cells : uncoupling of camp signal pathways2008

    • Author(s)
      Kajioka S, Nakayama S, Seki N, et al
    • Journal Title

      Journal of Pharmacology and Experimental Therapeutics 327

      Pages: 114-123

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RhoA/Rho kinase-mediated Ca_<2+> sensitization in the contraction of human prostate2007

    • Author(s)
      Takahashi R, Nishimura J, Seki N,Yunoki T, Tomoda T, Kanaide H, Naito S
    • Journal Title

      Neurourol Urodyn 26(4)

      Pages: 547-551

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] RhoA/Rho kinase-mediated Ca^<2+> sensitization in the contracdtion of human prostate2007

    • Author(s)
      Ryosuke Takahashi, et. al.
    • Journal Title

      Neurourology and Urodynamics 26

      Pages: 547-551

    • Related Report
      2007 Annual Research Report
  • [Journal Article] The profiles and patterns of detrusor overactiviey and their associationwith overactive bladder symptoms inmen with benign prostatic enlargement associated with detrusor overactivity

    • Author(s)
      Shahab N, Seki N, Takahashi R, Kajioka S, Takei M, Yamaguchi A, Naito S
    • Journal Title

      Neurourol Urodyn (in press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Presentation] Enhancement of the contractile responses of detrusor muscle by thrombin and trypsin in the cyclophosphamide-induced cystitismodel of mice2008

    • Author(s)
      Shiga K, Seki N, Naito S
    • Organizer
      第60回日本泌尿器科学会西日本総会
    • Place of Presentation
      北九州
    • Year and Date
      2008-11-17
    • Related Report
      2008 Final Research Report
  • [Presentation] Comprehensive studies of ATP-sensitive potassium channels in pig and human detrusor smooth muscle cells2008

    • Author(s)
      Seki N, Kajioka S, et.al.
    • Organizer
      International Continence Society 2008
    • Place of Presentation
      Egypt
    • Year and Date
      2008-10-20
    • Related Report
      2008 Final Research Report
  • [Presentation] RhoA/Rho kinase mediated Ca2^+ sensitization in the contraction of human prostate2008

    • Author(s)
      Takahashi R, Seki N, Naito S
    • Organizer
      The 3rd Pan-Pacific Continence SocietyMeeting
    • Place of Presentation
      Taiwan
    • Year and Date
      2008-09-26
    • Related Report
      2008 Final Research Report
  • [Presentation] RhoA/Rho kinase mediated Ca2+ sensitization in the contraction of human prostate.2008

    • Author(s)
      Takahashi R, Seki N, Naito S
    • Organizer
      The 3rd Pan-Pacific Continence Society Meeting
    • Place of Presentation
      Taiwan, Chang-GungMemorial Hospital
    • Year and Date
      2008-09-26
    • Related Report
      2008 Annual Research Report
  • [Presentation] 亜硝酸薬による膀胱排尿筋の逆説的収縮機構の解明2008

    • Author(s)
      梶岡俊一、関成人、高橋良輔、他
    • Organizer
      第15回日本排尿機能学会
    • Place of Presentation
      東京
    • Year and Date
      2008-09-12
    • Related Report
      2008 Final Research Report
  • [Presentation] 亜硝酸薬による膀胱排尿筋の逆説的収縮機構の解明.2008

    • Author(s)
      梶岡俊一, 関 成人, 他
    • Organizer
      第15回日本排尿機能学会
    • Place of Presentation
      東京 大手町サンヶィプラザ
    • Year and Date
      2008-09-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] ムスカリン受容体を介した排尿筋の持続性収縮における膜電流の包括的検討.2008

    • Author(s)
      関 成人, 梶岡俊一, 他
    • Organizer
      第96回日本泌尿器科学会総会
    • Place of Presentation
      横浜 パシフィコ横浜
    • Year and Date
      2008-04-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] ムスカリン受容体を介した排尿筋の持続性収縮における膜電流の包括的検討2008

    • Author(s)
      関成人、他
    • Organizer
      第96回日本泌尿器科学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2008-04-25
    • Related Report
      2008 Final Research Report
  • [Presentation] ヒト膀胱および前立腺におけるRho-kinaseの発現と機能2007

    • Author(s)
      関成人、他
    • Organizer
      第14回日本排尿機能学会
    • Place of Presentation
      福島
    • Year and Date
      2007-10-16
    • Related Report
      2008 Final Research Report
  • [Presentation] ヒト膀胱および前立腺におけるRho-kinaseの発現と機能2007

    • Author(s)
      関 成人
    • Organizer
      第14回日本排尿機能学会
    • Place of Presentation
      福島県猪苗代町
    • Year and Date
      2007-10-06
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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