Selection of new target genes to overcome the therapeutic resistance according to hypoxic condition,

• Project/Area Number: 19591863
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Yokohama City University
• Principal Investigator: KONDO Keiichi Yokohama City University, 市民総合医療センター, 准教授 (80363836)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2007: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: 腫瘍学 / 低酸素環境 / 悪性腫瘍 / HIF

Research Abstract

1. 腎癌及び膀胱癌細胞株においてHypoxia Inducible Factor (HIF) 1α& 2αそれぞれを強制発現または発現抑制させたクローンを作製しました。
2. HIFαを強制発現させると、低酸素環境下でも増殖が抑制されにくい傾向が認められました。
3. それぞれの癌細胞株に対して、酸素濃度依存性に感受性に差が報告されている薬剤(インターフェロン、テラルビシン、マイトマイシン)を作用させてみたところ、HIFαを強制発現させた細胞群に抵抗性が増加する傾向が見られました。

Research Products

• [Journal Article] Expression of Aquaporin 1 in Primary Renal Tumors : A Prognostic Indicator for Clear-Cell Renal Cell Carcinoma. (2008)
  • Author(s): Huang Y, Murakami T, Sano F, Kondo K, Nakaigawa N, Kishida T, Kubota Y, Nagashima Y, Yao M.
  • Journal Title: Eur Urol.
• [Journal Article] A three-gene expression signature model to predict clinical outcome of clear cell renal carcinoma (2008)
  • Author(s): Yao M, Huang Y, Shioi K, Hattori K, Murakami T, Sano F, Baba M, Kondo K, Nakaigawa N, Kishida T, Nagashima Y, Yamada-Okabe H, Kubota Y.
  • Journal Title: Int J Cancer. 123(5) Pages: 1126-32
• [Journal Article] A three-gene expression signature model to predict clinical outcome of clear cell renal carcinoma. (2008)
  • Author(s): Huang Y, Murakami T, Sano F, Kondo K, et. al
  • Journal Title: International Journal of Cancer 123(5) Pages: 1126-1132
  • Peer Reviewed
• [Journal Article] Identification and characterization of Birt-Hogg-Dube associated renal carcinoma. (2007)
  • Author(s): Murakami T, Sano F, Huang Y, Komiya A, Baba M, Osada Y, Nagashima Y, Kondo K, Nakaigawa N, Miura T, Kubota Y, Yao M, Kishida T.
  • Journal Title: J Pathol. 211(5) Pages: 524-31
• [Presentation] Effect of binding proteins to Hypoxia-lnducible Factor 1&alpha ; &2&alpha ; on growth of renal cell carcinoma. (2008)
  • Author(s): Keiichi Kondo
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] Effect of binding proteins to Hypoxia-Inducible Factor 1α& 2α on growth of renal cell carcinoma (2008)
  • Author(s): Kondo K
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋
• [Presentation] Role of hypoxia-inducible factor-alpha in chemo sensitivity of bladder cancer (2007)
  • Author(s): Kondo K
  • Organizer: 第66回日本癌学会総会
  • Place of Presentation: 横浜
• [Presentation] 膀胱癌の抗癌剤感受性におけるHypoxia-Inducible Factor(HIF)の関与 (2007)
  • Author(s): 近藤K
  • Organizer: 第45回日本癌治療学会総会
  • Place of Presentation: 京都