Novel molecular therapeutic strategies in prostate cancer

• Project/Area Number: 19591876
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Kinki University
• Principal Investigator: TANAKA Motoyoshi Kinki University, 医学部, 講師 (30347562)
• Co-Investigator(Kenkyū-buntansha): UEMURA Hirotsugu 近畿大学, 医学部, 教授 (90213397) ; NOZAWA Masahiro 近畿大学, 医学部, 講師 (00441080) ; DEVELASCO Marco 近畿大学, 医学部, 助教授 (20449838) ; 吉川 和宏 愛知医科大学, 医学部, 准教授 (60109759) ; 島田 啓司 奈良県立医科大学, 医学部, 講師 (90336850)
• Co-Investigator(Renkei-kenkyūsha): YOSHIKAWA Kazuhiro 愛知医科大学, 医学部, 准教授 (60109759) ; SHIMADA Keiji 奈良県立医科大学, 医学部, 講師 (90336850)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: 前立腺癌 / 動物モデル / 分子標的治療 / ペプチド

Research Abstract

我々はPTENという癌抑制伝子を中心に前立腺癌の研究を重ねてきた。今回樹立したPTEN遺伝子改変マウスは、前立腺における多段階発癌と徐睾術によりホルモン抵抗性前立腺癌-移行する前立腺癌発症モデルである。このモデルを中心に前立腺癌の発癌機序解明およびホルモン抵抗性獲得の分子機構の網羅的な解析を行った。また、選択的Cox2阻害薬(Meloxicam)による癌予防研究、さらに新規合成したペプチドによる創薬の有用性をこのモデルを用いて検証した。

Research Products

• [Journal Article] PTEN-Knockout Prostate Cancer as a Model for Experimental Immunotherapy (2009)
  • Author(s): Haga, K., Tomioka, A., Liao, C. P., Kimura, T., Matsumoto, H., Ohno, I., Hermann; K., Logg, C.R., Tanaka, Hirao, Y., Hong Wu, H., Kruse, C. A., Roy-Burman, P., Kasahara, N.
  • Journal Title: Journal of Urology 181 Pages: 354-362
  • Peer Reviewed
• [Journal Article] Delivery of PTEN via a novel gene microcapsule sensitizes prostate cancer cells to irradiation (2008)
  • Author(s): Tomioka, A., Tanaka, M., DeVelasco, M. Anai, S., Takada, S., Kushibiki, T., Tabata, Y., Rosser, C. J., Uemura, H., Hirao, H.
  • Journal Title: Molecular Cancer Therapeutics 7 Pages: 1864-1870
  • Peer Reviewed
• [Journal Article] GFP image analysis in the mouse orthotopic bladder cancer model (2008)
  • Author(s): DeVelasco, M., Tanaka, M., Anai, S., Tomioka, A., Nishio, K., Uemura, H.
  • Journal Title: Oncology Report 20 Pages: 537-542
  • Peer Reviewed
• [Journal Article] c-Jun NH2 terminal kinase activation and decreased expression of mitogen-activated protein kinase phosphatase-1 play important roles in invasion and angiogenesis of urothelial carcinomas (2007)
  • Author(s): Shimada, K., Nakamura, M., Ishida, E., Higuchi, T., Tanaka, M., Ota, I., Konishi, N.
  • Journal Title: American Journal of Pathology 171 Pages: 1003-1012
  • Peer Reviewed
• [Journal Article] Increased expression of cyclooxygenase-2 correlates with resistance to radiation in human prostate adenocarcinoma cells (2007)
  • Author(s): Anai, S., Tanaka, M., Shiverick, K. T., Kim, W., Takada, S., Boehlein, S., Goodison, S., Mizokami, A., Rosser, C. J.
  • Journal Title: Journal of Urology 177 Pages: 1913-1917
  • Peer Reviewed
• [Presentation] Pten gene targetingの手法を用いたマウス前立腺癌モデルとその臨床に向けた応用 (2008)
  • Author(s): 田中基幹
  • Organizer: 前立腺シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-12-13
• [Presentation] p16 peptide therapy radiosensitizes in prostate cancer orthotopic model (2008)
  • Author(s): Tanaka, M
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] Pten functional peptide therapy in prostate cancer (2008)
  • Author(s): Tanaka, M
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] PTEN機能性ペプチドによる前立腺癌治療の試み (2008)
  • Author(s): 田中基幹
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-10-29
• [Presentation] Prostate cancer mouse model derived by PTEN conditional gene targeting ; Towards pre-clinical appliocation (2008)
  • Author(s): Tanaka, M
  • Organizer: The 24^<th> Annual Meeting of Urological Research Society
  • Place of Presentation: Amsterdam (Netherlands)
  • Year and Date: 2008-09-19
• [Presentation] A novel PTEN functional peptide therapy for prostate cancer (2008)
  • Author(s): Tanaka, M
  • Organizer: 第14回日本遺伝子治療学会
  • Place of Presentation: 札幌
  • Year and Date: 2008-06-12
• [Presentation] A novel PTEN functional peptide therapy for prostate cancer (2008)
  • Author(s): M. Tanaka
  • Organizer: The 14^<th> Annual Meeting 2008 Japan Society of Gene Therapy
  • Place of Presentation: 札幌市
  • Year and Date: 2008-06-12
• [Presentation] PTEN機能ペプチドによる前立腺癌治療への応用 (2008)
  • Author(s): 田中基幹
  • Organizer: 第96回日本泌尿器科学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2008-04-25
• [Presentation] Pten functional peptide therapy in prostate cancer (2008)
  • Author(s): Tanaka, M
  • Organizer: The 99^<th> Annual Meeting, American Association for Cancer Research
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2008-04-15
• [Presentation] 4074 PTEN functional peptide therapy in prostate cancer (2008)
  • Author(s): M. Tanaka
  • Organizer: AACR Annual Meeting 2008
  • Place of Presentation: San Diego, USA
  • Year and Date: 2008-04-15
• [Presentation] Metabolic syndrome accelerates mouse prostate cancer development (2008)
  • Author(s): 田中基幹
  • Organizer: 第17回泌尿器分子・細胞研究会
  • Place of Presentation: 東京
  • Year and Date: 2008-02-16
• [Presentation] The role of PTEN in carcinogenesis of mouse prostate. (2007)
  • Author(s): Tanaka, M
  • Organizer: The 23^<rd> Annual Meeting of Urological Research Society, ct.
  • Place of Presentation: Napa (USA)
  • Year and Date: 2007-10-27
• [Presentation] 表在性膀胱癌マウスモデルを用いたリポソーム化ドキソルビシンの治療効果 (2007)
  • Author(s): 田中基幹
  • Organizer: 第16回日本腎泌尿器予防医学研究会
  • Place of Presentation: 大阪
  • Year and Date: 2007-07-13
• [Presentation] Intravesical instillation of liposomal doxorubicin nanoparticles in mouse superficial bladder cancer (2007)
  • Author(s): Tanaka, M
  • Organizer: The102^<nd> Annual Meeting, American Urological Association, Inc.
  • Place of Presentation: Anaheim (USA)
  • Year and Date: 2007-05-21
• [Presentation] Intravesical treatment of liposomal doxorubicin nanoparticles in mouse orthotopic bladder cancer model (2007)
  • Author(s): Tanaka, M
  • Organizer: The 98^<th> Annual Meeting, American Association for Cancer Research
  • Place of Presentation: Los Angeles USA
  • Year and Date: 2007-04-15
• [Presentation] The Role of PTEN Tumor Suppressor Gene in Prostate Cancer Derived by Prostate Specific Deletion of PTEN (2007)
  • Author(s): Tanaka, M
  • Organizer: The 10^<th>Annual Meeting of the American Society of Gene Therapy
  • Place of Presentation: Seattle USA
  • Year and Date: 2007-03-30