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Analysis of molecular mechanisms and further development of a new therapy for infertile patients with repeated implantation failure using autologous peripheral blood mononuclear cells

Research Project

Project/Area Number 19591894
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKyoto University

Principal Investigator

YOSHIOKA Shinya  Kyoto University, 医学研究科, 非常勤講師 (90333575)

Co-Investigator(Kenkyū-buntansha) FUJIWARA Hiroshi  京都大学, 医学研究科, 講師 (30252456)
NISHI Eiichiro  京都大学, 医学研究科, 准教授 (30362528)
TAKAO Yumi  京都大学, 医学研究科, 助教 (20447957)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords免疫細胞療法 / 不妊症 / 体外受精 / 着床不全 / 子宮内膜 / 着床 / 免疫細胞 / HCG / 胚 / リンパ球
Research Abstract

本研究により子宮内膜分化不全が原因となる着床障害の患者に対して、従来の内分泌学的なアプローチとは別に、患者本人の末梢血免疫細胞を用いた新しい治療法が開発され、実用化された。また本研究は、原因不明の着床障害患者に対する免疫機能異常を含んだ病態解明とその診断法および治療法の開発に新しい展開を導いた。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (34 results)

All 2009 2008 2007 Other

All Journal Article (20 results) (of which Peer Reviewed: 15 results) Presentation (11 results) Book (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] 免疫細胞を用いた着床率の向上2009

    • Author(s)
      藤原浩、高尾由美、小西郁生
    • Journal Title

      産婦人科治療 98巻

      Pages: 140-146

    • Related Report
      2008 Final Research Report
  • [Journal Article] 血液細胞を介した新しい胚-母体間の相互応答機構とそれを応用した着床不全患者に対する治療法の開発2009

    • Author(s)
      藤原浩
    • Journal Title

      日本産科婦人科・新生児血液学会誌 18巻

      Pages: 115-120

    • NAID

      10026709998

    • Related Report
      2008 Final Research Report
  • [Journal Article] Do circulating blood cells contribute to maternal tissue remodeling and embryo-maternal cross-talk around the implantation period2009

    • Author(s)
      Fujiwara H
    • Journal Title

      Mol Hum Reprod 15

      Pages: 335-343

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Is the zona pellucida an intrinsic source of signals activating maternal recognition of the developing mammalian embryo2009

    • Author(s)
      Fujiwara H, Araki Y, Toshimori K
    • Journal Title

      J Reprod Immunol (in press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Administration of bovine peripheral blood mononuclear cells into the uterine horn improve pregnancy rate following embryo transplantation2009

    • Author(s)
      Ideta A, Sakai S, Nakamura Y, Urakawa M, Hayama K, Tsuchiya K, Fujiwara H, Aoyagi Y
    • Journal Title

      Ani Reprod Sci (in press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Monoamine oxidase A is highly expressed by the human corpus uteum of pregnancy2008

    • Author(s)
      Takao Y, Fujiwara H, Yoshioka S, Fujii S, Ueda M
    • Journal Title

      Reproduction 136

      Pages: 367-375

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Emergence of highly neurofilamentimmunoreactive zipper-like axon segments at the transection site in scalpel-cordotomized adult rats2008

    • Author(s)
      Nishio K, Kawaguchi S, Fujiwara H
    • Journal Title

      Neuroscience 155

      Pages: 90-103

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 免疫細胞を用いた着床率向上の試み2008

    • Author(s)
      藤原浩
    • Journal Title

      日本産科婦人科学会雑誌 60巻

      Pages: 399-402

    • NAID

      110006943999

    • Related Report
      2008 Final Research Report
  • [Journal Article] Monoamine oxidase A is highly expressed by the human corpus luteum of pregnancy2008

    • Author(s)
      Takao, et al.
    • Journal Title

      Reproduction 136

      Pages: 367-375

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Emergence of highly neurofilament-immunoractive zipper-like axon segment at the transection site in scalpel-cordotomized adult rat.2008

    • Author(s)
      Nishio, et al.
    • Journal Title

      Neuroscience 155

      Pages: 90-103

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 免疫細胞を用いた着床率向上の試み2008

    • Author(s)
      藤原浩
    • Journal Title

      日本産科婦人科学会雑誌 60

    • NAID

      110006943999

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Platelet is a novel regulator for neovascularization and luteinization during human corpus luteum formation2007

    • Author(s)
      Furukawa K, Fujiwara H, Sato Y, Zeng BX, Fujii H, Yoshioka S, Nishi E, Nishio T
    • Journal Title

      Endocrinology 148

      Pages: 3056-3064

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Vascular endothelial growth factor production by circulating immune cells is elevated in ovarian hyperstimulation syndrome2007

    • Author(s)
      Kosaka K, Fujiwara H, Yoshioka S, Fujii S
    • Journal Title

      Hum Reprod 22

      Pages: 647-1651

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Integrin α5 is involved in fibronectin-induced human extravillous trophoblast invasion2007

    • Author(s)
      Zeng BX, Fujiwara H, Sato Y, Nishioka Y, Yamada S, Yoshioka S, Ueda M, Higuchi T, Fujii S
    • Journal Title

      J Reprod Immunol 73

      Pages: 1-10

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Membrane-bound peptidases regulate human extravillous trophoblast invasion2007

    • Author(s)
      Fujiwara H
    • Journal Title

      Trophoblast Res 21

      Pages: 70-75

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Laeverin/aminopeptidase Q, a novel bestatin- sensitive leucine aminopeptidase belonging to the M1family of aminopeptidases2007

    • Author(s)
      Maruyama M, Hattori A, Goto Y, Ueda M, Maeda M, Fujiwara H, Tsujimoto M
    • Journal Title

      J Biol Chem 282

      Pages: 20088-20096

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 胚着床による制御機構 ; 細胞間に反発力を誘導するEph-ephrinシグナルの関与2007

    • Author(s)
      藤井治子、藤原浩
    • Journal Title

      Hormone Frontier in Gynecology 14

      Pages: 2-5

    • Related Report
      2008 Final Research Report
  • [Journal Article] Integrin α5 is involved in fibronectin-induced human extravillous trophoblast invasion2007

    • Author(s)
      Bin-Xiang Zeng, et. al.
    • Journal Title

      Journal of Reproductive Immunology 73

      Pages: 1-10

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Membrane-Bound Peptidases Regulate Human Extravillous Trophoblast Invasion2007

    • Author(s)
      Fujiwara H
    • Journal Title

      PLACENTA 21

      Pages: 70-75

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Platelets Are Novel Regulators of Neovascularization and Luteinization during Human Corpus Luteum Formation2007

    • Author(s)
      Furukawa K, et. al.
    • Journal Title

      Endocrinology 148-7

      Pages: 3056-3064

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 難治性着床不全、不育症に対する治療の試み:自己血液細胞の応用日本生殖免疫学会2008

    • Author(s)
      藤原浩
    • Organizer
      第23回学術集会
    • Place of Presentation
      富山
    • Year and Date
      2008-12-06
    • Related Report
      2008 Final Research Report
  • [Presentation] 難治性着床不全、不育症に対する治療の試み : 自己血液細胞の応用2008

    • Author(s)
      藤原浩
    • Organizer
      日本生殖免疫学会第23回学術集会
    • Place of Presentation
      富山
    • Year and Date
      2008-12-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] Immune cell contribution to systemic crosstalk between mother and embryo around implantation period2008

    • Author(s)
      Fujiwara H
    • Organizer
      The 1st International workshop on Reproductive Biology": Recent Progresses in Reproductive Biology" SKLAB 2008
    • Place of Presentation
      Beijing, China
    • Year and Date
      2008-10-10
    • Related Report
      2008 Final Research Report
  • [Presentation] Immune cell contribution to systemic crosstalk between mother and embryo around implantation period2008

    • Author(s)
      H. Fujiwara
    • Organizer
      The 1st International workshop on Reproductive Biology : "Recent Progresses in Reproductive Biology" SKLAB 2008
    • Place of Presentation
      Beijing, China
    • Year and Date
      2008-10-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] 周産期免疫、常態と病態、着床誘導機構について日本産婦人科・新生児血液学会2008

    • Author(s)
      藤原浩
    • Organizer
      第18回学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2008-06-28
    • Related Report
      2008 Final Research Report
  • [Presentation] 周産期免疫、常態と病態、着床誘導機構について2008

    • Author(s)
      藤原浩
    • Organizer
      日本産婦人科・新生児血液学会第18回学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2008-06-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 免疫細胞を用いた着床率向上の試み2008

    • Author(s)
      藤原浩
    • Organizer
      第60回日本産科婦人科学学会学術集会卒後研修プログラム3. ARTの最近の話題
    • Place of Presentation
      横浜
    • Year and Date
      2008-04-14
    • Related Report
      2008 Final Research Report
  • [Presentation] 着床機構に関するOverview2008

    • Author(s)
      藤原浩
    • Organizer
      第3回生殖再生医学会シンポジウム着床に関する理論と実践
    • Place of Presentation
      東京
    • Year and Date
      2008-03-30
    • Related Report
      2008 Final Research Report
  • [Presentation] 特別講演「新しい母体-胚間の相互応答機構」2007

    • Author(s)
      藤原 浩
    • Organizer
      第45回日本生殖医学会東北支部会
    • Place of Presentation
      青森
    • Year and Date
      2007-11-10
    • Related Report
      2007 Annual Research Report
  • [Presentation] 細胞膜結合型ペプチダーゼによるヒト絨毛外栄養膜細胞浸潤の制御機構2007

    • Author(s)
      藤原浩
    • Organizer
      第12回病態と治療におけるプロテアーゼとインヒビター学会学術講演会シンポジウム
    • Place of Presentation
      大阪千里
    • Year and Date
      2007-08-04
    • Related Report
      2008 Final Research Report
  • [Presentation] Systemic crosstalk between mother and embryo around implantation period by immune and endocrine systems2007

    • Author(s)
      Fujiwara H
    • Organizer
      The 112nd Annual Meeting of The Japanese Association of Anatomists(日米合同シンポジウム)
    • Place of Presentation
      Osaka
    • Year and Date
      2007-03-27
    • Related Report
      2008 Final Research Report
  • [Book] 排卵・月経の機序産婦人科学テキスト(倉智博久、吉村泰典(編))2007

    • Author(s)
      藤原浩(分担執筆)
    • Publisher
      中外医学社
    • Related Report
      2008 Final Research Report
  • [Book] 月経困難症の取り扱いスーパーローテート・マニュアル(朝倉啓文、小西郁生、末岡浩、田中忠夫、堤治(編))2007

    • Author(s)
      藤原浩(分担執筆)
    • Publisher
      金原出版
    • Related Report
      2008 Final Research Report
  • [Patent(Industrial Property Rights)] 絨毛外栄養膜細胞特異的蛋白質

    • Related Report
      2008 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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