Gene expression analysis of single human oocyte and preembryo, and its application to clinical infertility

• Project/Area Number: 19591910
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Keio University
• Principal Investigator: KUJI Naoaki Keio University, 医学部, 講師 (80169987)
• Co-Investigator(Kenkyū-buntansha): HAMATANI Toshio 慶應義塾大学, 医学部, 講師 (60265882) ; YOSHIMURA Yasunori 慶應義塾大学, 医学部, 教授 (10129736) ; KATO Shingo 慶應義塾大学, 医学部, 講師 (10177446) ; MIZUSAWA Yuri 慶應義塾大学, 医学部, 助教 (10348716)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 移植・再生医療 / 医療・福祉 / 遺伝子 / 遺伝学

Research Abstract

本研究ではマウス単一卵子から抽出したtotal RNAをRibo-SPIA法にて遺伝子増幅した場合の、遺伝子発現解析結果の信頼性を検討した。卵子に高頻度に発現する4遺伝子(H1foo, GAPDH, βActin, Eef1a)は、単一卵子からの微少RNAをサンプルとしても高い再現性を持って線形に増幅され、この増幅法により加齢による卵子の遺伝子発現変化を解析可能であった。

Research Products

• [Journal Article] Expression of human oocyte-specific linkere histone protein and its incorporation into sperm chromatin during fertilization (2009)
  • Author(s): Mizusawa Y, Kuji N, Tanaka Y, Tanaka M, Ikeda E, Komatsu S, Kato S, Yoshimura Y
  • Journal Title: Fertil Steril(Epub ahea of print) 13
  • Peer Reviewed
• [Journal Article] Expression of human oocyte-specific linker histone protein and its incorporation into sperm chromatin during fertilization. (2009)
  • Author(s): Mizusawa Y, Kuji N, Tanaka Y, Tanaka M, Ikeda E, Komatsu Kato S, Yoshimura Y.
  • Journal Title: Fertil Steril. 13(Epub ahead of print)
  • Peer Reviewed
• [Journal Article] What can we learn from gene expression profiling of mouse oocytes? (2008)
  • Author(s): Hamatani, T, Yamada M, Kkutsu H, Kuji N, Mochimaru Y, Takano M, Toyoda M, Miyado K, Umezawa A, Yoshimura Y.
  • Journal Title: Reproduction 135(5) Pages: 581-592
  • Peer Reviewed
• [Journal Article] Buoyant density and sedimentation dynamics of HIV-1 in two density-gradient media for semen processing (2008)
  • Author(s): Kuji N, Yoshii T, Hamatani T, Hanabusa H, Yoshimura Y, Kato S
  • Journal Title: Fertil Steril 90(5) Pages: 1983-1987
  • Peer Reviewed
• [Journal Article] What can we learn from gene expression profiling of mouse oocytes? . (2008)
  • Author(s): Hamatani T, Yamada M, Akutsu Kuii N, Mochimaru Y, Takano M, Toyoda M, Miyado K, Umezawa A Yoshimura Y.
  • Journal Title: Reproduction. 135(5) Pages: 581-592
  • Peer Reviewed
• [Journal Article] Buoyant density and sedimentation dynamics of HIV-1in two density-gradient media for semen processing. (2008)
  • Author(s): Kuji N, Yoshii T, Hamatani T, Hanabusa H, Yoshimura Y, Kato S.
  • Journal Title: Fertil Steril. 90(5) Pages: 1983-1987
  • Peer Reviewed