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Gene expression analysis of single human oocyte and preembryo, and its application to clinical infertility

Research Project

Project/Area Number 19591910
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKeio University

Principal Investigator

KUJI Naoaki  Keio University, 医学部, 講師 (80169987)

Co-Investigator(Kenkyū-buntansha) HAMATANI Toshio  慶應義塾大学, 医学部, 講師 (60265882)
YOSHIMURA Yasunori  慶應義塾大学, 医学部, 教授 (10129736)
KATO Shingo  慶應義塾大学, 医学部, 講師 (10177446)
MIZUSAWA Yuri  慶應義塾大学, 医学部, 助教 (10348716)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords移植・再生医療 / 医療・福祉 / 遺伝子 / 遺伝学
Research Abstract

本研究ではマウス単一卵子から抽出したtotal RNAをRibo-SPIA法にて遺伝子増幅した場合の、遺伝子発現解析結果の信頼性を検討した。卵子に高頻度に発現する4遺伝子(H1foo, GAPDH, βActin, Eef1a)は、単一卵子からの微少RNAをサンプルとしても高い再現性を持って線形に増幅され、この増幅法により加齢による卵子の遺伝子発現変化を解析可能であった。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (6 results)

All 2009 2008

All Journal Article (6 results) (of which Peer Reviewed: 6 results)

  • [Journal Article] Expression of human oocyte-specific linkere histone protein and its incorporation into sperm chromatin during fertilization2009

    • Author(s)
      Mizusawa Y, Kuji N, Tanaka Y, Tanaka M, Ikeda E, Komatsu S, Kato S, Yoshimura Y
    • Journal Title

      Fertil Steril(Epub ahea of print) 13

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of human oocyte-specific linker histone protein and its incorporation into sperm chromatin during fertilization.2009

    • Author(s)
      Mizusawa Y, Kuji N, Tanaka Y, Tanaka M, Ikeda E, Komatsu Kato S, Yoshimura Y.
    • Journal Title

      Fertil Steril. 13(Epub ahead of print)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] What can we learn from gene expression profiling of mouse oocytes?2008

    • Author(s)
      Hamatani, T, Yamada M, Kkutsu H, Kuji N, Mochimaru Y, Takano M, Toyoda M, Miyado K, Umezawa A, Yoshimura Y.
    • Journal Title

      Reproduction 135(5)

      Pages: 581-592

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Buoyant density and sedimentation dynamics of HIV-1 in two density-gradient media for semen processing2008

    • Author(s)
      Kuji N, Yoshii T, Hamatani T, Hanabusa H, Yoshimura Y, Kato S
    • Journal Title

      Fertil Steril 90(5)

      Pages: 1983-1987

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] What can we learn from gene expression profiling of mouse oocytes? .2008

    • Author(s)
      Hamatani T, Yamada M, Akutsu Kuii N, Mochimaru Y, Takano M, Toyoda M, Miyado K, Umezawa A Yoshimura Y.
    • Journal Title

      Reproduction. 135(5)

      Pages: 581-592

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Buoyant density and sedimentation dynamics of HIV-1in two density-gradient media for semen processing.2008

    • Author(s)
      Kuji N, Yoshii T, Hamatani T, Hanabusa H, Yoshimura Y, Kato S.
    • Journal Title

      Fertil Steril. 90(5)

      Pages: 1983-1987

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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