Development of central auditory systemand and plasticity of inner ear in congenital deaf model rat

• Project/Area Number: 19591961
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Kanazawa University
• Principal Investigator: ITO Makoto Kanazawa University, 医学系, 准教授 (50283106)
• Co-Investigator(Kenkyū-buntansha): TUKATANI Tosiaki 金沢大学, 医学系, 助教 (20303307)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 先天聾モデル動物 / 聴覚中枢 / カルシウム結合蛋白 / 虚血再灌流障害 / ビタミンE / 先天聾モデル / 発達 / プログラム細胞死 / 脳の可塑性 / カルバインD / パルブアルブミン / 虚血性内耳障害モデル / 虚血再灌流臆害

Research Abstract

先天聾モデルラットを作成し、聴覚中枢の投射と蛋白発現の変化について検討した。下位中枢では下丘投射の減少がみられたが、上位中枢では大きな変化はみられなかった。カルシウム結合蛋白の発現に変化がみられ、Hearing onsetが起きないということが、聴覚中枢の発達に影響をおよぼす事が明らかとなった。また突発性難聴において、抗酸化ビタミンEとCを従来の治療に併用する事で、著明な治癒率の向上が得られた。これは虚血再灌流障害を予防する効果による可能性がある。

Research Products

• [Journal Article] Retrograde Mastoidectomy on Demand with Soft-Wall Reconstruction in Pediatric Cholesteatoma. (2010)
  • Author(s): Miyako Hatano, Makoto Ito, Tomokazu Yoshizaki
  • Journal Title: Acta Otolaryngol 129(in press)
  • NAID: 120002439386
  • Peer Reviewed
• [Journal Article] A case of chronic otitis media caused by Mycobacterium abscessus (2010)
  • Author(s): Sugimoto H, Ito M, Hatano M, Nakanishi Y, Maruyama Y, Yoshizaki T.
  • Journal Title: Auris Nasus Larynx (In press)
  • NAID: 120002029020
  • Peer Reviewed
• [Journal Article] Retrograde Mastoidectomy on Demand with Soft-Wall Reconstruction in Pediatric Cholesteatoma (2010)
  • Author(s): Miyako Hatano, Makoto Ito, Tomokazu Yoshizaki
  • Journal Title: Acta Otolaryngol (In press)
  • NAID: 120002439386
  • Peer Reviewed
• [Journal Article] Anatomical projections of the nuclei of the lateral lemniscus in the albino rat (Rattus norvegicus). (2009)
  • Author(s): Jack B. Kelly, Brian A. van Adel, Makoto Ito
  • Journal Title: J Comp neurol 512 Pages: 573-593
  • Peer Reviewed
• [Journal Article] Neuroprotective Effects of Vitamin E on Adult Rat Motoneurons Following Facial Nerve Avulsion. (2009)
  • Author(s): Shigeru Hoshida, Miyako Hatano, Mitsuru Furukawa, Makoto Ito
  • Journal Title: Acta Otolaryngol 129 Pages: 330-336
  • Peer Reviewed
• [Journal Article] Changes in Calbindin-D28k and Parvalbumin Expression in Auditory Brainstem Nuclei following Unilateral Cochlear Ablation in Neonatal Rat. (2009)
  • Author(s): Miyako Hatano, Mitsuru Furukawa, Makoto Ito
  • Journal Title: Acta Otolaryngol 129 Pages: 839-845
  • Peer Reviewed
• [Journal Article] Changes in Calbindin-D28k and Parvalbumin Expression in Auditory Brainstem Nuclei following Unilateral Cochlear Ablation in Neonatal Rat (2009)
  • Author(s): Miyako Hatano, Mitsuru Furukawa, Makoto Ito
  • Journal Title: Acta Otolaryngol 129 Pages: 839-845
  • Peer Reviewed
• [Journal Article] Effects of Japanese Herbal Medicine Juzen-taiho-to in otitis-prone children-a preliminary study (2009)
  • Author(s): Yumiko Maruyama, Shigeru Hoshida, Mitsuru Frukawa, Makoto Ito
  • Journal Title: Acta Otolaryngol 129 Pages: 14-18
  • Peer Reviewed
• [Journal Article] Prognostic factors for squamous cell carcinoma of the temporal bone : extensive bone involvement or extensive soft tissue involvement? (2009)
  • Author(s): Makoto Ito, Miyako Hatano, Tomokazu Yoshizaki
  • Journal Title: Acta Otolaryngol 129 Pages: 1313-1319
  • NAID: 120001728439
  • Peer Reviewed
• [Journal Article] Neuroprotective Effects of Vitamin E on Adult Rat Motoneurons Following Facial Nerve Avulsion (2009)
  • Author(s): S Hoshida, M Hatano, M Furukawa, M Ito
  • Journal Title: Acta Otolaryngologica 129 Pages: 330-336
  • Peer Reviewed
• [Journal Article] Changes in Calbindin-D28K and Parvalbumin Expression in Auditory Brainstem Nuclei following Unilateral Cochlear Ablation in Neonatal Rat (2009)
  • Author(s): M Hatano, M Furukawa M Ito
  • Journal Title: Acta Otolaryngologica 129(In press)
  • Peer Reviewed
• [Journal Article] Anatomical projections of the nuclei of the lateral lemniscus in the albino rat (Rattus norvegicus) (2009)
  • Author(s): JB. Kelly, BA Adel, M Ito
  • Journal Title: Journal of Comparative Neurology 512 Pages: 573-593
  • Peer Reviewed
• [Journal Article] Anatomical plasticity in brainstem auditory nuclei following unilateral ablation of the inferior colliculus in neonatal rats. (2008)
  • Author(s): Ito M, Hatano M, Okoyama S, Kelly JB
  • Journal Title: 239 Pages: 92-98
  • NAID: 120001135641
  • Peer Reviewed
• [Journal Article] Vitamin E and Vitamin C in Treatmentof Idiopathic Sudden Sensorineural Hearing Loss. (2008)
  • Author(s): Miyako Hatano, Naoki Uramoto, Yozo Okabe, Mitsuru Frukawa, Makoto Ito
  • Journal Title: Acta Otolaryngol 128 Pages: 116-121
  • Peer Reviewed
• [Journal Article] Anatomical plasticity in brainstem auditory nuclei following unilateral ablation of the inferior colliculus in neonatal rats (2008)
  • Author(s): Ito M, Hatano M, Okoyama S, Kelly JB
  • Journal Title: Hearing Research 239 Pages: 92-98
  • NAID: 120001135641
  • Peer Reviewed
• [Journal Article] Vitamin E and Vitamin C in Treatmentof Idiopathic Sudden Sensorineural Hearing Loss (2008)
  • Author(s): M Hatano, N Uramoto, Y Okabe, Frukawa, and M Ito
  • Journal Title: Acta Otolaryngol 128 Pages: 116-121
  • Peer Reviewed
• [Journal Article] Neuroprotective Effects of TJ-23 on Adult Rat Motoneurons Following Peripheral Facial Nerve Axotomy. (2007)
  • Author(s): M Ito, M Ohbayashi, M Furukawa, S Okoyama
  • Journal Title: Otolaryngol Head Neck Surg 136 Pages: 225-230
  • Peer Reviewed
• [Journal Article] Neuroprotective Effects of TJ-23 on Adult Rat Motoneurons Following Periheral Facial Nerve Axotomy (2007)
  • Author(s): M Ito, M Ohbayashi, M Furukawa, S Okoyama
  • Journal Title: Otolaryngol Head Neck Surg 136 Pages: 225-230
  • Peer Reviewed
• [Presentation] Anatomical plasticity in brainstem auditory nuclei to inferior colliculus following Unilateral Cochlear Ablation in Neonatal Rat. (2010)
  • Author(s): Eriko Shima, Miyako Hatano, Kazuya Kurita, Tomokazu Yoshizaki, Makoto Ito
  • Organizer: 33rd ARO midwinter meeting
  • Place of Presentation: Disneyland Hotel Anaheim (USA)
• [Presentation] Clonal spread of beta lactamase producing, amoxicillin clavulanate resistant Strains of Non typeable Haemophilus influenzae (BLPACR) among Young Children Attending a Day Care Center in Japan. (2010)
  • Author(s): Kazuya Kurita, Eriko Shima, Tomokazu Yoshizaki, Makoto Ito
  • Organizer: 33rd ARO midwinter meeting
  • Place of Presentation: Disneyland Hotel Anaheim (USA)
• [Presentation] Tomokazu Yoshizaki Analysis of Mechanism of Malignancy Enhanced by Epitherial Mesenchimal Transition in Squamous Cell Carcinoma of the Temporal Bone. (2010)
  • Author(s): Hisashi Sugimoto, Makoto Ito
  • Organizer: 33rd ARO midwinter meeting
  • Place of Presentation: Disneyland Hotel Anaheim (USA)
• [Presentation] Anatomical plasticity in brainstem auditory nuclei to inferior colliculus following Unilateral Cochlear Ablation in Neonatal Rat (2010)
  • Author(s): Eriko Shima, Miyako Hatano, Kazuya Kurita, Tomokazu Yoshizaki, Makoto Ito
  • Organizer: 33rd ARO midwinter meeting
  • Place of Presentation: カリフォルニア ディズニーランドホテル(米国)
• [Presentation] Clonal spread of beta-lactamase-producing, amoxicillin-clavulanate-resistant Strains of Non-typeable Haemophilus influenzae(BLPACR)among Young Children Attending a Day Care Center in Japan (2010)
  • Author(s): Kazuya Kurita, Eriko Shima, Tomokazu Yoshizaki, Makoto Ito
  • Organizer: 33rd ARO midwinter meeting
  • Place of Presentation: カリフォルニア ディズニーランドホテル(米国)
• [Presentation] Analysis of Mechanism of Malignancy Enhanced by Epitherial-Mesenchimal Transition in Squamous Cell Carcinoma of the Temporal Bone (2010)
  • Author(s): Hisashi Sugimoto, Makoto Ito, Tomokazu Yoshizaki
  • Organizer: 33rd ARO midwinter meeting
  • Place of Presentation: カリフォルニア ディズニーランドホテル(米国)
• [Presentation] 乳幼児上咽頭細菌叢の変化とこれからの中耳炎治療 (2009)
  • Author(s): 伊藤真人
  • Organizer: 第57回耳鼻咽喉科手術支援システム・ナビ研究会 ランチョンセミナー
  • Place of Presentation: 石川県教育会館(石川県)
  • Year and Date: 2009-11-14
• [Presentation] 耳鼻咽喉科領域感染症中耳炎についての最近の話題第57回西日本化学療法学会 (2009)
  • Author(s): 伊藤真人
  • Organizer: 第52回中日本感染症学会合同学会ワークショップ
  • Place of Presentation: 名古屋国際会館(愛知県)
• [Presentation] 上気道感染症と咽頭細菌叢保育園児の上咽頭細菌叢の変遷 (2009)
  • Author(s): 伊藤真人
  • Organizer: 第22回日本口腔咽頭科学会シンポジウム
  • Place of Presentation: ホテルアパローム紀国(和歌山県)
• [Presentation] 上気道感染症と咽頭細菌叢 保育園児の上咽頭細菌叢の変遷 (2009)
  • Author(s): 伊藤真人
  • Organizer: 第22回日本口腔咽頭科学会 シンポジウム
  • Place of Presentation: ホテルアパローム紀の国・和歌山県民文化会館(和歌山県)
• [Presentation] 耳鼻咽喉科領域感染症 中耳炎についての最近の話題 (2009)
  • Author(s): 伊藤真人
  • Organizer: 第57回西日本化学療法学会 第52回中日本感染症学会合同学会 ワークショップ
  • Place of Presentation: 名古屋国際会議場(愛知県)
• [Presentation] Inner ear fistula in cholesteatoma surgery. (2008)
  • Author(s): Miyako Hatano, Makoto Ito
  • Organizer: 8th International conference on Cholesteatoma and ear surgery.
  • Place of Presentation: アンタルヤ(Turky)
• [Presentation] Treatment of temporal bone squamous cell carcinoma. (2008)
  • Author(s): Makoto Ito, M Hatano
  • Organizer: 8th International conference on Cholesteatoma and ear surgery
  • Place of Presentation: アンタルヤ(Turky)
• [Presentation] Vitamin E and Vitamin C in the Treatment of Idiopathic Sudden Sensorineural Hearing Loss. (2008)
  • Author(s): Makoto Ito, Yozo Okabe, Mitsuru Furukawa, Miyako Hatano
  • Organizer: The 12th Japan-Koria Joint Meeting of Otorhinolaryngology Head and Neck Surgery
  • Place of Presentation: 奈良市公会堂(奈良県)
• [Presentation] Vitamin E and Vitamin C in the Treatment of Idiopathic Sudden Sensorineural Hearing Loss (2008)
  • Author(s): M Ito, Y Okabe, M Furukawa, M Hatano
  • Organizer: The 12th Japan-Korea Joint Meeting of Otor hinolaryngology Head and Neck Surgery
  • Place of Presentation: 奈良市
• [Presentation] Studies of labyrinthine fistulas : report of 27 cases (2008)
  • Author(s): Miyako Hatano, Makoto Ito
  • Organizer: 8th International conference on Cholesteatoma & ear surgery
  • Place of Presentation: トルコ、アンタルヤ
• [Presentation] ラット片側先天聾モデルにおける下丘への投射の変化 (2008)
  • Author(s): 波多野都, 伊藤真人, 古川仭
  • Organizer: 第109回日本耳鼻咽喉科学会総会
  • Place of Presentation: 大阪市
• [Presentation] 新生児期ラット下丘剥離後の脳幹部聴覚路の形態学的可塑性について(第2報) (2008)
  • Author(s): 伊藤真人, 波多野都
  • Organizer: 第17回日本耳科学会総会
  • Place of Presentation: 神戸市
• [Presentation] ラット先天聾モデルにおける下丘への投射の変化 (2008)
  • Author(s): 波多野都, 伊藤真人
  • Organizer: 第17回日木耳科学会総会
  • Place of Presentation: 神戸市
• [Presentation] Changes in Calbindin-D28k and Parvalbumin Expression in Auditory Brainstem Nuclei following Unilateral Cochlear Ablation in Neonatal Rat. (2007)
  • Author(s): Miyako Hatano, Makoto Ito
  • Organizer: AAO-HNSF Annual Meeting. (American Asociation of Otolaryngology)
  • Place of Presentation: Washington DC (USA)
• [Presentation] Protective Effects of Vitamin E After Facial Nerve Injury. (2007)
  • Author(s): Makoto Ito, Miyako Hatano
  • Organizer: AAO-HNSF Annual Meeting 2007 (American Asociation of Otolaryngology)
  • Place of Presentation: Washington DC (USA)
• [Presentation] Protective Effects of Vitamin E After Facial Nerve Injury (2007)
  • Author(s): Makoto Ito, Miyako Hatano
  • Organizer: AAO-HNSF Annual Meeting
  • Place of Presentation: ワシントンDC
• [Presentation] Changes in Calbindin-D28k and Parvalbumin Expression in A uditory Brainstem Nuclei following Unilateral Cochlear Ablation in Neonatal Rat (2007)
  • Author(s): Miyako Hatano, Makoto Ito
  • Organizer: AAO-HNSF Annual Meeting
  • Place of Presentation: ワシントンDC
• [Book] よくわかる病態生理14耳鼻咽喉科疾患 (2009)
  • Author(s): 伊藤真人
  • Publisher: 日本医事新報社
• [Book] 急性中耳炎治療入門 (2009)
  • Author(s): 伊藤真人
  • Publisher: 金原出版
• [Book] よくわかる病態生理14 耳鼻咽喉科疾患 (2009)
  • Author(s): 伊藤真人
  • Total Pages: 8
  • Publisher: 日本医事新報社
• [Book] 今日の耳鼻咽喉科・頭頸部外科治療指針第3版 (2008)
  • Author(s): 伊藤真人
  • Publisher: 医学書院
• [Remarks] ホームページ等
  • URL: http://web.kanazawa-u.ac.jp/~med31/