Disturbances of the stria vascularis in mice with genetic abnormalities

• Project/Area Number: 19591963
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Nagoya University
• Principal Investigator: SONE Michihiko Nagoya University, 大学院・医学系研究科, 准教授 (30273238)
• Co-Investigator(Kenkyū-buntansha): NAKASHIMA Tsutomu 名古屋大学, 大学院・医学系研究科, 教授 (30180277)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 蝸牛 / 血管条 / 内耳障害 / 難聴 / メラニン / 加齢

Research Abstract

Our findings are the first quantitative evidence to show an age-related overexpression of cochlear melanin and an alteration in the proportion of eumelanin and pheomelanin with aging, suggesting a possible otoprotective function of eumelanin against age-related cochlear deterioration. We focused on the roles of midkine and pleiotrophin in the stria vascularis using mice deficient in these genes. Hearing thresholds examined by auditory brain stem response were significantly higher in midkine knockout and pleiotrophin knockout mice than in wild-type mice. Double knockout mice showed higher thresholds compared to midkine knockout and pleiotrophin knockout mice. The expression of Kir4.1 was weak in the knockout mice and severe vacuolar degeneration was observed by electron microscopy in the intermediate cells of the double knockout mice. The present study demonstrates that midkine and pleiotrophin are essential components for the morphological maintenance of intermediate cell in the stria vascularis.

Research Products

• [Journal Article] (2008)
  • Author(s): Midkine, Pleiotrophin遺伝子欠損による血管条障害、曾根三千彦、中島務
  • Journal Title: Audiology Japan 51 Pages: 291-292
• [Journal Article] Midkine, Pleiotrophin遺伝子欠損による血管条障害 (2008)
  • Author(s): 曾根三千彦, 中島務
  • Journal Title: Audiology Japan 51 Pages: 291-292
  • NAID: 10025595403
• [Journal Article] C57BL/6マウスにおける蝸牛メラニン量の加齢変化についての検討 (2007)
  • Author(s): 林秀雄、曽根三千彦、中島務
  • Journal Title: Otology Japan 17 Pages: 290-290
  • NAID: 10021297278
• [Journal Article] Comparison of the quantity of cochlear melanin in young and old C57BL/6 mice. (2007)
  • Author(s): Hayashi H, Sone M, Schachern PA, Wakamatsu K, Paparella MM, Nakashima T, et al, (他3名)
  • Journal Title: Arch Otolaryngol Head Neck Surg 133 Pages: 151-154
  • Peer Reviewed
• [Journal Article] Comparison of the quantity of cochlear melanin in young and old C57BL/6 mice (2007)
  • Author(s): Hideo Hayashi, Michihiko Sone, Tsutomu Nakashima, et. al.
  • Journal Title: Arch Otolaryngol Head Neck Surg 133 Pages: 151-154
  • Peer Reviewed
• [Presentation] Midkine, Pleiotrophin遺伝子欠損による血管条障害 (2008)
  • Author(s): 曽根三千彦、中島務
  • Organizer: 第53回日本聴覚医学会総会
  • Place of Presentation: 東京
  • Year and Date: 2008-10-02
• [Presentation] Midkine, Pleiotrophin遺伝子欠損による血管条障害 (2008)
  • Author(s): 曾根三千彦, 中島務
  • Organizer: 第53回日本聴覚医学会総会
  • Place of Presentation: 明治記念館
  • Year and Date: 2008-10-02
• [Presentation] C57BL/6マウスにおける蝸牛メラニン量の加齢変化についての検討 (2007)
  • Author(s): 林秀雄、曽根三千彦、中島務
  • Organizer: 第17回日本耳科学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2007-10-18
• [Presentation] C57BL/6マウスにおける蝸牛メラニン量の加齢変化についての検討 (2007)
  • Author(s): 林 秀雄、曾根 三千彦、中島 務
  • Organizer: 第17回日本耳科学会総会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2007-10-18