| Project/Area Number |
19591979
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
|
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| Research Institution | Oita University |
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Principal Investigator |
SUZUKI Masashi Oita University, 医学部, 教授 (60211314)
|
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| Co-Investigator(Kenkyū-buntansha) |
HIRANO Takashi 大分大学, 医学部, 講師 (20305056)
KODAMA Satoru 大分大学, 医学部, 講師 (40325717)
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| Project Period (FY) |
2007 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 上気道 / 粘膜免疫 / ワクチン / 粘膜粘液 / 樹状細胞 |
|---|
| Research Abstract |
Dendritic cells (DCs) are essential for the induction of antigen (Ag)-specific immune responses, DC-targeted vaccination might be an effective strategy for the induction of the specific immune responses. Flt3 ligand (Flt3L) mobilizes and stimulates myeloid and lymphoid progenitor cells, and DCs. The purpose of this study is to investigate the effect of Flt3L on the induction of Ag-specific immune responses in nasal mucosa. Mice were administered with Flt3L 10Hg intranasally or intraperitoneally, on day 0. After the Flt3L application, mice were further immunized with P6 outer membrane protein of nontypeable Haemophilus influenzae (NTHi) 10μg on day 6, 13, and 20, and mice were killed on day 27. Control were not given Flt3L. The number and subset of DCs in nasal-associated lymphoid tissue (NALT) were analyzed by flow cytometry. P6-specific antibody titers in nasal wash and serum were measured by enzyme-linked immunosorbent assay (ELISA), and P6-specific antibody-producing cells were examined by enzyme-linked immunospot (ELISPOT) assay. CD11c^+ DCs increased in NALT after nasal Flt3L administration. In addition, P6-specific nasal IgA and systemic IgG titers were significantly elevated in nasally Flt3L-treated mice, possessing higher number of the specific IgA-producing in nasal mucosa. This study showed that nasal but not peritoneal application with a single dose of Flt3L induced increase of NALT DCs, resulted in enhanced local and systemic immune responses. These findings suggest that nasal application with Flt3L might be a useful vaccine strategy.
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