frontier treatment for anosmia using olfactory stem cell

• Project/Area Number: 19591986
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Nagoya City University
• Principal Investigator: HAMAJIMA Yuki Nagoya City University, 大学院・医学研究科, 助教 (30343403)
• Co-Investigator(Kenkyū-buntansha): HIDA Hideki 名古屋市立大学, 大学院・医学研究科, 教授 (00305525)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 嗅覚障害 / 組織幹細胞 / 嗅上皮細胞

Research Abstract

胎児マウス嗅上皮粘膜より、組織幹細胞を取り出した。浮遊細胞は球状なneuro-sphere を形成し、12ヶ月以上継代することが出来た。マウス骨髄からも同様にsphereを形成する培養細胞が分離できたが、2ヶ月程すると、細胞はアポトーシスを起こし、消失していったのに対し、鼻粘膜由来の細胞は15ヶ月継代することができた。これらの細胞からRNA を抽出し、RT-PCRにて神経系マーカーの発現をみると、Musashi1、Nestin、などの神経幹細胞を示唆する遺伝子の発現を認めた

Research Products

• [Journal Article] 小児気管支異物摘出における工夫 (2009)
  • Author(s): 森田真理、濱島有喜、渡邉暢浩、村上信五
  • Journal Title: 30(1) Pages: 42-46
  • NAID: 10024844545
  • Peer Reviewed
• [Journal Article] Directed differentiation of mouse cochlear neural progenitors in vitro (2008)
  • Author(s): Lin J, Feng L, Hamajima Y, Komori M, Burns TC, Fukudome S, Anderson J, Wang D, Verfaillie CM, Low WC.
  • Journal Title: Am J Physiol Cell Physiol 296(3)
  • Peer Reviewed
• [Journal Article] Type and severity of patulous Eustachian tube Nakazawa (2008)
  • Author(s): M, Inagaki A, Hamajima Y, et.al.
  • Journal Title: Nagoya med 49 Pages: 167-178
  • Peer Reviewed
• [Journal Article] Identification of Id1 in acquired middle ear cholesteatoma (2008)
  • Author(s): Zhang Q, Hamajima Y, Zhang Q, Lin J
  • Journal Title: Arch Otolaryngology-HNS 134(3) Pages: 306-310
  • Peer Reviewed
• [Journal Article] 【異物 初期対応から摘出まで】耳鼻咽喉科領域の異物 (2008)
  • Author(s): 渡邉暢浩, 濱島有喜, 稲垣彰 ENTONI
  • Journal Title: 摘出術の実際 96 Pages: 15-21
  • Peer Reviewed
• [Journal Article] Directed differentiation of mouse cochlear neural progenitors in vitro. (2008)
  • Author(s): Lin J
  • Journal Title: Am J Physiol Cell Physiol. 296(3)
  • Peer Reviewed
• [Journal Article] Identification of Idl in acquired middle ear cholesteatoma (2008)
  • Author(s): Zhang Q
  • Journal Title: Arch Otolaryngology-HNS 134(3) Pages: 306-310
  • Peer Reviewed
• [Journal Article] Increase in dopaminergic neurons from mouse embryonic stem cell-derived neural progenitor/stem cells is mediated by hypoxia inducible factor-lalpha. (2008)
  • Author(s): Kim TS
  • Journal Title: J Neurosci RES 86(11) Pages: 2353-2362
  • Peer Reviewed
• [Journal Article] Cochlear stem cells/progenitors and degenerative hearing disorders (2007)
  • Author(s): Lin J, Feng L, Fukudome S, Hamajima Y, Huang T, Levine S
  • Journal Title: Curr Med Chem 14(27) Pages: 2937-43
  • Peer Reviewed
• [Journal Article] Expression of Syk is associated with nasal polyp in patients with allergic rhinitis (2007)
  • Author(s): Hamajima Y, Fujieda S, Subaga H, Yamada T, Moribe K, Watanabe N, Murakami S
  • Journal Title: Auris Nasas Larynx 34(1) Pages: 49-56
  • Peer Reviewed
• [Journal Article] Id1 Induces the Proliferation of Cochlear Sensory epithelial cells via the NF-кB/Cyclin D1 Pathway in vitro (2007)
  • Author(s): Ozeki M, Hamajima Y, Feng L, Ondrey F, Schlentz E, Lin J
  • Journal Title: J neurosci Res . Feb 15 85(3) Pages: 515-24
  • Peer Reviewed
• [Journal Article] Pneumococcal Peptidoglycan-Polysaccharides Induce the Expression of IL-8 in Airway Epithelial Cells via NF-κB, NF-IL6, and/or AP-1 dependent (2007)
  • Author(s): Tsuchiya K, Toyama K, Tsuprun V, Hamajima Y, Kim Y, Ondrey F, Lin J
  • Journal Title: Laryngoscope, Jan 117(1) Pages: 86-91
  • Peer Reviewed
• [Journal Article] スギ花粉症における第二世代抗ヒスタミン薬の有用性の検討 (2007)
  • Author(s): 濱島有喜、中村善久、大野伸晃、大橋卓、服部綾、高木繁、村上信五
  • Journal Title: 診療と新薬 44(2)
  • Peer Reviewed
• [Journal Article] 好酸球中耳炎update (2007)
  • Author(s): 渡邉暢浩、濱島有喜、中村善久、村上信五
  • Journal Title: ENTONI 73 Pages: 51-57
  • Peer Reviewed
• [Journal Article] スギ花粉症における第二世代抗ヒスタミン薬の臨床効果 (2007)
  • Author(s): 藤枝重治、山田武千代、濱島有喜
  • Journal Title: 他 日鼻誌 46(1) Pages: 18-28
  • Peer Reviewed
• [Journal Article] マクロライド系抗菌薬クラリスロマイシンの鼻茸への移行性 (2007)
  • Author(s): 羽柴基之、濱島有喜
  • Journal Title: 他耳展 50 Pages: 89-94
  • NAID: 130003975413
  • Peer Reviewed
• [Journal Article] 好酸球中耳炎update 3.乳突洞削開術と鼓室形成術 (2007)
  • Author(s): 渡邉暢浩、濱島有喜、中村善久、村上信五
  • Journal Title: ENTONI 73 Pages: 51-57
  • Peer Reviewed
• [Journal Article] スギ花粉症における第二世代抗ヒスタミン薬の有用性の比較検討 (2007)
  • Author(s): 濱島有喜、中村善久
  • Journal Title: 他診療と新薬 44(2) Pages: 177-186
  • Peer Reviewed
• [Journal Article] スギ花粉症に対する第二世代抗ヒスタミン薬の効果 (2007)
  • Author(s): 大野伸晃 濱島有喜 大橋卓 村上信五
  • Journal Title: 耳鼻咽喉科免疫アレルギー 25巻2号 Pages: 213-214
  • Peer Reviewed
• [Journal Article] 【花粉症治療のアップデート】受療行動を高める薬剤選択 (2007)
  • Author(s): 今野昭義, 榎本雅夫, 濱島有喜, 久保伸夫MEDICO
  • Journal Title: 38巻2号 Pages: 51-62
  • Peer Reviewed
• [Journal Article] 中耳杯細胞分化の制御因子 (2007)
  • Author(s): 中村善久, 濱島有喜, 村上信五
  • Journal Title: 耳鼻咽喉科免疫アレルギー 25巻2号 Pages: 71-72
  • NAID: 10021297295
  • Peer Reviewed
• [Journal Article] 3DAccu-i-tomo(小照射野コーンビームCT)の耳科手術支援機器として利用 (2007)
  • Author(s): 村上信五, 濱島有喜, 稲垣彰
  • Journal Title: 耳鼻咽喉科展望 50巻5号 Pages: 359-361
  • Peer Reviewed
• [Journal Article] Expression of Syk is associated with nasal polyp in patients with allergic rhinitis (2007)
  • Author(s): Hamajima Y
  • Journal Title: Auris Nasus Larynx 34 Pages: 49-56
  • Peer Reviewed
• [Journal Article] Pneumococcal Peptidoglycan-polysaccharides induce the Expressi on of Interlukin-8 in Airway Epithelial Cells by way of Nuclear Factor interleukin-6, or Activation Protein-1 dependent Mechanisms (2007)
  • Author(s): Tsuchiya K
  • Journal Title: Laryngoscope 117 Pages: 86-91
  • Peer Reviewed
• [Journal Article] スギ花粉症における第2世代抗ヒスタミン薬の有用性の比較検討 (2007)
  • Author(s): 濱島有喜
  • Journal Title: 診療と新薬 44(2) Pages: 177-186
  • Peer Reviewed
• [Presentation] 尋常性乾癬における扁桃腺摘出術の効果 (2007)
  • Author(s): 濱島有喜
  • Organizer: 第20回日本口腔・咽頭科学会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-09-06