Neuron-Like Differentiation and Selective Ablation of Undifferentiated Embryonic Stem Cells Containing Suicide Gene with Oct-4 Promoter

• Project/Area Number: 19592012
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Gifu University
• Principal Investigator: HARA Akira Gifu University, 大学院・医学系研究科, 教授 (10242728)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 胚性肝細胞 / ES細胞 / 神経組織再生 / 奇形腫 / 自殺遺伝子 / thymidine kinase / ganciclovir human / 胚性幹細胞 / ganciclovir / human

Research Abstract

研究成果が5編の論文として国際雑誌に掲載された。これら論文には、基礎研究としての海馬神経細胞、眼球光彩色素細胞の研究も含まれる。OCT-4遺伝子プロモーター下流に自殺遺伝子thymidine kinase を挿入したES細胞(OCT-TK-ES細胞)を3次元培養し、ganciclovir投与により選択的に未分化ES細胞の増殖を抑制し、神経分化蛋白発現の変化を検索し、ganciclovir投与により未分化細胞成分のみが除去され、神経分化を示すことが示された。

Research Products

• [Journal Article] Spatiotemporal expression of Hsp20 and its phosphorylation in hippocampal CA1 pyramidal neurons following transient forebrain ischemia (2009)
  • Author(s): Niwa M, Hara A, Taguchi A, Aoki H, Kozawa O, Mori H
  • Journal Title: Neurol Res (in press)
  • Peer Reviewed
• [Journal Article] Marked increases in hippocampal neuron indoleamine 2, 3-dioxygenase via IFN-γ-independent pathway following transient global ischemia inmouse (2009)
  • Author(s): Hoshi M, Saito K, Murakami Y, Taguchi A, Fujigaki H, Tanaka R, Takemura M, Ito H, Hara A, Seishima M.
  • Journal Title: Neurosci Res 63 Pages: 194-198
  • Peer Reviewed
• [Journal Article] Localization and spatiotemporal expression of IDO following transient forebrain ischemia in gerbils (2008)
  • Author(s): Taguchi A, Hara A, Saito K, Hoshi M, Niwa M, Seishima M, Mori H.
  • Journal Title: Brain Res 1217 Pages: 78-85
  • Peer Reviewed
• [Journal Article] Transplantation of melanocytes into iris: Method for iris repigmentation (2008)
  • Author(s): Aoki H, Yoshida H, Hara A, Suzuk T, Kunisada T.
  • Journal Title: Transplantation 85 Pages: 492-494
  • Peer Reviewed
• [Journal Article] Neuron-like Differentiation and Selective Ablation of Undifferentiated Embryonic Stem Cells Containing Suicide Gene with Oct-4 Promoter (2008)
  • Author(s): Hara A, Aoki H, Taguchi A, Niwa M, Yamada Y, Kunisada T, Mori H
  • Journal Title: Stem Cells Dev 17 Pages: 619-628
  • Peer Reviewed
• [Journal Article] Iris as a recipient tissue for pigment cells: organized in vivo differentiation of melanocytes and pigmented epithelium derived from embryonic stem cells in vitro (2008)
  • Author(s): Aoki H, Hara A, Motohashi T, Chem H, Kunisada T
  • Journal Title: Dev Dynam 237 Pages: 2394-2404
  • Peer Reviewed
• [Journal Article] Neuron-like differentiation and selective ablation of undifferen-tiated embryonic stem cells containing suicide gene with Oct-4 promoter (2008)
  • Author(s): A Hara, H Aoki, A Taguchi, M Niwa, Y Yamada, T Kunisada, H Mori
  • Journal Title: Stem Cells and Development 17 Pages: 619-628
  • Peer Reviewed
• [Journal Article] Iris as a recipient tissue for pigment cells : organized in vivo differentiation of melanocytes and pigmented epithelium derived fromembryonic stem cells in vitro. (2008)
  • Author(s): Aoki H, Hara A, Motohashi T, Chem H, Kunisada T
  • Journal Title: Dev Dynam 237 Pages: 2394-2404
  • Peer Reviewed
• [Journal Article] Localization and spatiotemporal expression of IDO following transient forebrain ischemia in gerbils. (2008)
  • Author(s): Taguchi A, Hara A, Saito K, Hoshi M, Niwa M, Seishima M, Mori H
  • Journal Title: Brain Res 1217 Pages: 78-85
  • Peer Reviewed
• [Journal Article] Neuron-like Differentiation and Selective A blation of Undifferentiated Embryonic Stem Cells Containing Suicide Gene with Oct-4 Promoter (2008)
  • Author(s): A Hara, H Aoki, A Taguchi, M Niwa, Y Yamada, T Kunisada, H Mori
  • Journal Title: Stem Cells and Development (印刷中)
  • Peer Reviewed
• [Presentation] Neuronal differentiation and control of teratoma formation in transplanted embryonic stem cells using retinal neurodegenerative mouse model (2009)
  • Author(s): 原明
  • Organizer: Symposium : Molecular pharmacological understandings of neurodegenerative disorders. The spring international convention, organized by The Pharmaceutical Society of Korea
  • Place of Presentation: 韓国, Daejeon
  • Year and Date: 2009-05-07
• [Presentation] Methotrexate induces neuronal differentiation of human embryonic stem (ES) cells transplanted into nude mouse retina (2008)
  • Author(s): 原明、田口紋子、山田泰広、森秀樹
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] Methotrexate induces neuronal differentiation of human embryonicstem (ES) cells transplanted into nude mouse retina. (2008)
  • Author(s): 原明, 田口紋子, 山田泰広, 森秀樹
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-29
• [Presentation] 未分化ES細胞の選択的除去によるES細胞腫瘍化抑制と神経分化 (2008)
  • Author(s): 原 明、田口紋子、山田泰広、青木仁美、國貞隆弘、森 秀樹
  • Organizer: 第7回日本再生医療学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-03-14
• [Presentation] Selective ablation of undifferentiated embryonic stem cells containing suicide gene with Oct-4 promoter (2007)
  • Author(s): 原明、田口紋子、尾山武、山田泰広、森秀樹
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-02
• [Presentation] Supression of tumorigenicity and induction of neuronal differentiation in embryonic stem cells transplanted in the adult mouse retina by folate antagonist methotrexate (2007)
  • Author(s): 原明
  • Organizer: AACR Annual Meeting
  • Place of Presentation: Los Angeles
  • Year and Date: 2007-04-15
• [Book] OCT-3/4 Expressing Cells as Cancer Stem Cells in Human Immature Teratoma : Cancer Differentiation Potential(New Cell Differentiation Research Topics)(Saitama H) (2008)
  • Author(s): Hara A, Oka N, Aoki H, Taguchi A, Yamada Y, Niwa M, Mori H
  • Publisher: Nova Science Publish
• [Book] New research on neuronal networks. In : Neuronal Network Research Horizons (2008)
  • Author(s): Hara A, Oka N, Aoki H, Taguchi A, Yamada Y, Niwa M, Mori H.
  • Publisher: Nova Science Publish
• [Remarks]
  • URL: http://wwwl.gifu-u.ac.jp/~pathol/12009.html
• [Remarks]
  • URL: http://www1.gifu-u.ac.jp/~patho1/12008.html