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Development of CGRP-gene-transfected immune regulatory cells for treatment of refractory uveoretinitis in human

Research Project

Project/Area Number 19592041
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionTokyo Medical University

Principal Investigator

KEZUKA Takeshi  Tokyo Medical University, 医学部, 講師 (00287137)

Co-Investigator(Kenkyū-buntansha) TAKEUCHI Masaru  東京医科大学, 医学部, 准教授 (40260939)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsCGRP / 遺伝子導入細胞 / 難治性ぶどう膜炎 / 制御性細胞 / 神経ペプチド / 難治性視神経炎 / ぶどう膜炎 / 遺伝子治療 / 樹状細胞
Research Abstract

難治性ぶどう膜炎患者由来樹状細胞を用いた神経ペプチド (CGRP) 遺伝子の導入と、その免疫学的解析を行った。健常者の単核球同様、CGRP 遺伝子導入細胞からのCGRP 産生は40-60pg/mlであった。難治性ぶどう膜炎を持つベーチェット病患者から得られたCGRP 遺伝子導入細胞由来の培養上清中サイトカインは、対照群と比べて有意差を見いだせなかった。CGRP 刺激樹状細胞表面分子で免疫調節に関与する接着分子を検討したが、有意な結果が得られなかった。これらのことから、正常細胞を用いたCGRP 遺伝子導入実験では、制御性細胞を作成できるが、ベーチェット病患者由来の細胞では免疫制御細胞を誘導しなかった。今後、他の難治性ぶどう膜炎でも同様に検討する必要がある。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (19 results)

All 2009 2008 2007

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (5 results) Book (1 results)

  • [Journal Article] Angiotensin II type 1 receptor blocker telmisartan suppresses experimental autoimmune uveitis2009

    • Author(s)
      Okunuki Y, Usui Y, Nagai N, Kezuka T, Ishida S, Takeuchi M, Goto H
    • Journal Title

      Invest Ophthalmol Vis Sci (in press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human corneal endothelial cells expressing programmed death-ligand 1 (PD-L1) suppress PD-1+ T helper 1 cells by a contact-dependent mechanism2009

    • Author(s)
      Sugita S, Usui Y, Horie S, Futagami Y, Yamada Y, Ma J, Kezuka T, Hamada H, Usui T, Mochizuki M, Yamagami S
    • Journal Title

      Invest Ophthalmol Vis Sci 50

      Pages: 263-72

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human corneal endothelial cells expressing programmed death-ligand 1 (PD-L1) suppress PD-1+ T helper 1 cells by a contact-dependent mechanism.2009

    • Author(s)
      Sugita S
    • Journal Title

      Invest Ophthalmol Vis Sci. 50

      Pages: 263-272

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Acute retinal necrosis in Japan2008

    • Author(s)
      Usui Y, Takeuchi M, Goto H, Mori H, Kezuka T, Sakai J, Usui M
    • Journal Title

      Ophthalmology 115

      Pages: 1632-3

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Increased levels of monokine induced by interferon-gamma (Mig) in the vitreous of patients with diabetic retinopathy2008

    • Author(s)
      Wakabayashi Y, Usui Y, Okunuki Y, Takeuchi M, Kezuka T, Iwasaki T, Goto H
    • Journal Title

      Diabet Med 25

      Pages: 875-7

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic surveillance of retinal autoantigens in endogenous uveitis : implication of esterase D and brain-type creatine kinase as novel autoantigens2008

    • Author(s)
      Okunuki Y, Usui Y, Kezuka T, Hattori T, Masuko K, Nakamura H, Yudoh K, Goto H, Usui M, Nishioka K, Kato T, Takeuchi M
    • Journal Title

      Mol Vis 14

      Pages: 1094-104

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional expression of B7H1 on retinal pigment epithelial cells2008

    • Author(s)
      Usui Y, Okunuki Y, Hattori T, Kezuka T, Keino H, Ebihara N, Sugita S, Usui M, Goto H, Takeuchi M
    • Journal Title

      Exp Eye Res 86

      Pages: 52-9

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Circulating neutrophils in Behcet disease is resistant for apoptotic cell death in the remission phase of uveitis2008

    • Author(s)
      Fujimori K, Oh-i K, Takeuchi M, Yamakawa N, Hattori T, Kezuka T, Keino H, Suzuki J, Goto H, Sakai J, Usui M
    • Journal Title

      Graefes Arch Clin Exp Ophthalmol 246

      Pages: 285-90

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional expression of B7H1 on retinal pigment epithelial cells2008

    • Author(s)
      Usui, Y
    • Journal Title

      Experimental Eye Research 82

      Pages: 52-59

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Circulating neutrophils in Behcet disease is resistant for apoptotic cell death in the remission phase of uveitis2008

    • Author(s)
      Fujimori, K
    • Journal Title

      Graefes Arch Clin Exp Ophthalmol. 246

      Pages: 285-290

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic surveillance of retinal autoantigens in endogenous uveitis : implication of esterase D and brain-type creatine kinase asnovel autoantigens2008

    • Author(s)
      Okunuki, Y
    • Journal Title

      Molecular Vision 14

      Pages: 1094-1104

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased levels of monokine induced by interferon-gamma (Mig) in the vitreous of patients with diabetic retinopathy.2008

    • Author(s)
      Wakabayashi Y
    • Journal Title

      Diabet Med. 25

      Pages: 875-877

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic surveillance of autoimmunity in Behcet's disease with uveitis: selenium binding protein is a novel autoantigen in Behcet's disease2007

    • Author(s)
      Okunuki Y, Usui Y, Takeuchi M, Kezuka T, Hattori T, Masuko K, Nakamura H, Yudoh K, Usui M, Nishioka K,Kato T
    • Journal Title

      Exp Eye Res 84

      Pages: 823-31

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Presentation] ラット胎児胸腺移植ヌードマウスを用いた自己免疫生ぶどう膜炎の発症メカニズムの解析2008

    • Author(s)
      竹内大、服部貴明、臼井嘉彦、奥貫陽子、馬娟、山川直之、張リナ、毛塚剛司、後藤浩、田口修
    • Organizer
      第112回日本眼科学会
    • Place of Presentation
      横浜
    • Year and Date
      2008-04-17
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Gene therapy with calcitonin gene-related peptide suppresses murine experimental autoimmune uveoretinitis2008

    • Author(s)
      Kezuka, T. et al
    • Organizer
      ARVO 2008
    • Related Report
      2008 Final Research Report
  • [Presentation] The mechanisms by which spontaneous development of organ-localized autoimmune diseases induced by xenogenic thymus2008

    • Author(s)
      Takeuchi M, Hattori T, Usui Y, Okunuki Y, Ma J, Yamakawa N, Zhang T, Kezuka T, Goto H, Taguchi O
    • Organizer
      ARVO 2008
    • Related Report
      2008 Final Research Report
  • [Presentation] Gene therapy with calcitonin gene-related peptide suppresses murine experimental autoimmune uveoretinitis.2008

    • Author(s)
      Kezuka, T., et al.
    • Organizer
      ARVO 2008
    • Place of Presentation
      米国 フロリダ
    • Related Report
      2008 Annual Research Report
  • [Presentation] The mechanisms by which spontaneous development of organ-localized autoimmune diseases induced by xenogenic thymus.2008

    • Author(s)
      Takeuchi M
    • Organizer
      ARVO2008
    • Place of Presentation
      Florida U. S. A
    • Related Report
      2008 Annual Research Report
  • [Book] Acute retinal necrosis(92, Review)2007

    • Author(s)
      Kezuka T, Atherton SS
    • Publisher
      Chem Immunol Allergy
    • Related Report
      2008 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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