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Effect of durotaxis-dependent cell movement in wound on development of keloid

Research Project

Project/Area Number 19592068
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plastic surgery
Research InstitutionChiba University

Principal Investigator

YOSHIMOTO Shinya  Chiba University, 大学院・医学研究院, 准教授 (90220748)

Co-Investigator(Kenkyū-buntansha) UDAGAWA Akikazu  千葉大学, 医学部・付属病院, 講師 (70323425)
NOMURA Jun  千葉大学, 教育学部, 准教授 (30252886)
SUGITA Katuo  千葉大学, 教育学部, 教授 (40211304)
一瀬 正治  千葉大学, 大学院・医学研究院, 教授 (90082156)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords創傷治癒学 / ケロイド / デュロタキシス / メカニカルストレス / 創傷治癒 / 繊維我細胞 / テトラスパニン / 表皮角化細胞 / 繊維芽細胞 / テトラスパニンウェッブ / スクラッチモデル / 皮膚由来線維芽細胞 / MAPキナーゼ / 細胞外マトリックス
Research Abstract

Keloids are skin fibrotic conditions that can be caused by minor insults to skin, such as acne or ear piercing, or by severe injuries such as burns. Keloids are characterized by an overabundant deposition of extra-cellular matrix such as collagen, and their histologic morphology has a characteristic manner. Keloid occurs more often on chest, shoulder or elbow-joint etc. which is easily affected by mechanical stretch. So we consider that the response to mechanical stress of fibroblast derived from Keloid may be different from that of normal skin fibroblast. We analyzed the sensitivity to mechanical stress using cell stretch apparatus (NC-500) which has silicon chambers on which the fibroblasts are cultured and scratched (We called wound repair scratch model). We found that wound repair is deffernted vertical and horizontary scratch. Furthermore knockdown of CD151 and EWI-F tetraspanin web moles induced detachment of cells from a culture apparatus.
This effect is higher in keratinocytes than in keroid fibroblasts and normal fibroblasts. Therefore, we suggest that tetraspanin web moles affect skin wound healing.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (23 results)

All 2010 2009 2008 2007 Other

All Journal Article (15 results) (of which Peer Reviewed: 14 results) Presentation (4 results) Book (4 results)

  • [Journal Article] Cyclical cell stretching of skin-derived fibroblasts downregulates connective tissue growth factor (CTGF) production2009

    • Author(s)
      Kanazawa Y, Nomura J, Yoshimoto S, Ichinose M
    • Journal Title

      Connective Tissue Resarch No50

      Pages: 322-329

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Treatment of velopharyngeal inadequacy in a patient with submucous cleft palate and myasthenia gravis2009

    • Author(s)
      Rikihisa N, Udagawa A, Yoshimoto S, Ichinose M
    • Journal Title

      Cleft Palate Craniofacial Journal No46

      Pages: 558-562

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cyclical cell stretching of skin-derived fibroblasts downregulates connective tissue growth factor(CTGF)production2009

    • Author(s)
      Kanazawa Y, Nomura J, Yoshimoto S, Ichinose M
    • Journal Title

      Connective Tissue Resarch 50

      Pages: 323-329

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Treatment of velopharyngeal inadequacy in a patient with submucous Cleft palate and myasthenia gravis2009

    • Author(s)
      Rikihisa N, Udagawa A, Yoshimoto S, Ichinose M
    • Journal Title

      Cleft Palate Craniofacial Journal 46

      Pages: 558-562

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A case of accessory scrotom with perineal lipoma2009

    • Author(s)
      Harada M, Udagawa A, Yoshimoto S, Ichinose M.
    • Journal Title

      J Plastic Reconstractive Surgery 62

      Pages: 108-109

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Low-dose GH supplementation reduces the TLR-2 and TNF-alpha expressinon in visceral fat2008

    • Author(s)
      Kubota Y, Unoki, H, Bujo H, Udagawa A, Yoshimoto S
    • Journal Title

      Biochemical and Biophysivcal Resarch Communications No368

      Pages: 81-87

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] DNA methylation changes during cleft palate formation induced by retinoic acid in mice2008

    • Author(s)
      Kuriyama M, Udagawa A, Yoshimoto S, Ichinose M
    • Journal Title

      Cleft Palate Craniofacial Journal No45

      Pages: 545-551

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Low-dose GH supplementation reduces the TLR2 and TNF-alpha expressions in visceral fat.2008

    • Author(s)
      Kubuta Y,Unoki H,Bujo H, Udagawa A,Yoshimoto S.
    • Journal Title

      Biochemical and Biophysical Resarch Communications 368

      Pages: 81-87

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA methylation changes during cleft palate formation induced by retinoic acid in mice.2008

    • Author(s)
      Kuriyama M, Udagawa A, Yoshioto S,ichinose M.
    • Journal Title

      Cleft Palate Craniofacial Journal 45

      Pages: 545-551

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tessier number 7 cleft with oblique clefts of bilateral soft palates and rare symmetric structure of zygomatic arch.2008

    • Author(s)
      Kuriyama M, Udagawa A, Yoshimoto S, Ichinose M
    • Journal Title

      J piastic Reconstructive Aesthetic Surgery 61

      Pages: 447-450

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A simple reconstruction for congential unilateral lower lip plasty2007

    • Author(s)
      Udagawa A, Arikawa K, Shimizu S, Yoshimoto S, Ichinose M
    • Journal Title

      Plastic and Reconstructive Surgery No120

      Pages: 238-244

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Primary repair in adult with untreated cleft lip-cleft palate2007

    • Author(s)
      Morioka D, Yoshimoto S, Udagawa A, Okubo F, Yoshikawa A
    • Journal Title

      Plastic and Reconstructive Surgery No120

      Pages: 1981-1988

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A simple reconstruction for congenital unilateral lower Iip Palsy2007

    • Author(s)
      Udagawa A., Arikawa K, Shimizu S, Yoshimoto S, Ichinose M.
    • Journal Title

      Plastic and Reconstructive Surgery 120

      Pages: 238-244

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Primary repair in adult patients with untreated cleft lip-cleft palate2007

    • Author(s)
      Morioka D, Yoshimoto S, Udagawa A, Ohkkubo F, Yoshikawa A.
    • Journal Title

      Plastic and Reconstructive Surgery 120

      Pages: 1981-1988

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tessier number 7 cleft with oblique clefts of bilateral soft palates and rare symmetric structure of zygomatic arch

    • Author(s)
      Kuriyama M, Udagawa A, Yoshimoto S, Ichinose M
    • Journal Title

      J Plastic reconstructive Aesthetic surgery No61

      Pages: 447-450

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Presentation] 皮膚創傷治癒におけるテトラスパニンウェブ関連タンパクの細胞接着制御の役割2009

    • Author(s)
      深谷佳孝, 吉本信也, ほか
    • Organizer
      日本形成外科基礎学術集会
    • Place of Presentation
      東京
    • Year and Date
      2009-10-08
    • Related Report
      2009 Final Research Report
  • [Presentation] 皮膚創傷治癒におけるテトラスパニンウェブ関連タンパクの細胞接着制御の役割2009

    • Author(s)
      深谷佳孝、吉本信也, ほか
    • Organizer
      日本形成外科基礎学術集会
    • Place of Presentation
      東京(新宿)
    • Year and Date
      2009-10-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] ケロイド由来皮膚線維芽細胞を用いた In vitro 創傷治癒モデルにおけるメカニカルストレスの影響2008

    • Author(s)
      野村純, 金沢雄一郎, 吉本信也, ほか
    • Organizer
      ケロイド・肥厚性瘢痕研究会
    • Place of Presentation
      東京
    • Year and Date
      2008-03-08
    • Related Report
      2009 Final Research Report
  • [Presentation] ケロイド由来皮膚線維芽細胞を用いたinVitro,創傷治癒モデルにおけるメカニカルストレスの影響2008

    • Author(s)
      野村 純、 金沢 雄一郎、 石坂 美穂、 宇田川 晃一、 吉本 信也、 一瀬 正治
    • Organizer
      第14回ケロイド・肥厚性癜痕研究会
    • Place of Presentation
      東京
    • Year and Date
      2008-03-08
    • Related Report
      2007 Annual Research Report
  • [Book] 感染症・合併症ゼロをめざす創閉鎖2010

    • Author(s)
      炭山嘉伸, 有馬陽一, 吉本信也
    • Total Pages
      205
    • Publisher
      羊土社
    • Related Report
      2009 Final Research Report
  • [Book] 感染症・合併症ゼロをめざす創閉鎖2010

    • Author(s)
      炭山嘉伸 有馬陽一 吉本信也, 他
    • Total Pages
      205
    • Publisher
      羊土社
    • Related Report
      2009 Annual Research Report
  • [Book] ビジュアル基本手技シリーズ 確実に身に付く! 縫合・局所麻酔 創に応じた適切な縫合法の選択と手技のコツ2009

    • Author(s)
      落合武徳, 清水孝徳, 吉本信也
    • Total Pages
      141
    • Publisher
      羊土社
    • Related Report
      2009 Final Research Report
  • [Book] ビジュアル基本手技シリーズ 確実に身に付く!縫合・局所麻酔 創に応じた適切な縫合法の選択と手技のコツ2009

    • Author(s)
      落合武徳、清水孝徳、吉本信也
    • Total Pages
      141
    • Publisher
      羊土社
    • Related Report
      2008 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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