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Study of three-dimensional bone formation induced with adipose tissue derived stem cells

Research Project

Project/Area Number 19592081
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plastic surgery
Research InstitutionKansai Medical University

Principal Investigator

KUSUMOTO Kenji  Kansai Medical University, 医学部, 教授 (20161630)

Co-Investigator(Kenkyū-buntansha) ONISHI Sayuri  関西医科大学, 医学部, 助教 (50440970)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords生物・生物工学 / 移植・再生医療 / バイオテクノロジー / 脂肪組織 / 幹細胞 / 骨 / 骨新生 / 再建 / 生物・生体工学 / 再生医学
Research Abstract

Human adipose-derived stem cells (ASCs) have the capacity to regenerate and the potential to differentiate into multiple lineages of mesenchymal cells, bone, cartilage, and fat cells. The ASC-loaded honeycomb collagen scaffolds cultured for 14 days were subcutaneously transplanted into nude mice, and excised after 8 weeks. Bone formation in vivo was examined using HE stain, von Kossa stain, and osteocalcin immunostain. These results suggest that this carrier is a suitable scaffold for ASCs and will be useful as a three-dimensional bone tissue engineering scaffold in vitro and in vivo.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report

Research Products

(24 results)

All 2009 2008 2007 Other

All Journal Article (9 results) (of which Peer Reviewed: 5 results) Presentation (15 results)

  • [Journal Article] Platelet-rich plasma : the importance of platelet separation and concentration2009

    • Author(s)
      Kakudo N, Kushida S, Kusumoto K
    • Journal Title

      Plast.Reconstr.Surg. 123

      Pages: 1135-1136

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proliferation-promoting effect of platelet-rich plasma on human adipose-derived stem cells and human dermal fibroblasts.2008

    • Author(s)
      Kakudo N, Minakata T, Mitsui T, Kushida S, Notodihardjo FZ, Kusumoto K
    • Journal Title

      Plast Reconstr Surg 122(5)

      Pages: 1352-1360

    • Related Report
      2009 Final Research Report
  • [Journal Article] Bone tissue engineering using human adipose-derived stem cells and honeycomb collagen scaffold.2008

    • Author(s)
      Kakudo N, Shimotsuma, A, Miyake S, Kushida S, Kusumoto K
    • Journal Title

      Journal of Biomedical Materials Research. Part A 84(1)

      Pages: 191-197

    • Related Report
      2009 Final Research Report
  • [Journal Article] ヒト脂肪組織由来幹細胞の分離と脂肪・骨・軟骨への分化-幹細胞の供給源としての脂肪組織-2008

    • Author(s)
      覚道奈津子, 鈴木健司, 櫛田哲史, 楠本健司
    • Journal Title

      関西医科大学雑誌 59(2-4)

      Pages: 169-176

    • NAID

      130004625734

    • Related Report
      2009 Final Research Report
  • [Journal Article] Fibroblast growth factor-2 stimulates adipogenic differentiation of human adipose-derived stem cells.2008

    • Author(s)
      Kakudo N, Shimotsuma A, Kusumoto K
    • Journal Title

      Biochemical and Biophysical Research Communications 359(2)

      Pages: 239-244

    • NAID

      130003374575

    • Related Report
      2009 Final Research Report
  • [Journal Article] Proliferation-promoting effect of platelet-rich plasma on human adipose-derived stem cells and human dermal fibroblasts2008

    • Author(s)
      Kakudo N, Minakata T, Mitsui T, Kushida S, Notodihardjo FZ, Kusumoto K.
    • Journal Title

      Plast. Reconstr. Surg. 122

      Pages: 1342-1360

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bone tissue engineering using human adipose-derived stem cells and honeycomb collagen scaffold2008

    • Author(s)
      Kakudo N, Shimotsuma A, Miyake S, Kushida S, Kusumoto K
    • Journal Title

      J Biomed Mater Res A 84

      Pages: 191-197

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fibroblast growth factor-2 stimulates adipogenic differentiation of human adipose-derived stem cells2007

    • Author(s)
      Kakudo N, Shimotsuma A, Kusumoto K.
    • Journal Title

      Biochem.Biophys.Res.Commun 359

      Pages: 239-24

    • NAID

      130003374575

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ヒト脂肪組織由来幹細胞の分離と脂肪・骨・軟骨への分化-幹細胞の供給源としての脂肪組織

    • Author(s)
      覚道 奈津子、鈴木 健司、櫛田 哲史、楠本 健司
    • Journal Title

      関西医科大学雑誌 (印刷中)

    • NAID

      130004625734

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Effects of collagen gel contraction with TGF-β1 and PRP in embedded culture of adipose-derived stem cells of dermal fibroblasts2009

    • Author(s)
      Satoshi Kushida, Natsuko Kakudo, Kenji Kusumoto
    • Organizer
      The 7th Annual Meeting of the International Federation for Adipose Therapeutics and Science
    • Place of Presentation
      Daegu, Korea, Hotel Inter Burgo
    • Year and Date
      2009-10-15
    • Related Report
      2009 Final Research Report
  • [Presentation] Possibility of myofibroblast differentiation of human adipose-derived stem cell.2009

    • Author(s)
      Natsuko Kakudo, Satoshi Kushida, Kenji Kusumoto
    • Organizer
      The 7th Annual Meeting of the International Federation for Adipose Therapeutics and Science
    • Place of Presentation
      Daegu, Korea, Hotel Inter Burgo
    • Year and Date
      2009-10-15
    • Related Report
      2009 Final Research Report
  • [Presentation] Effects of collagen gel contraction with TGF-β1 and PRP in embedded culture of adipose-derived stem cells of dermal fibroblasts2009

    • Author(s)
      櫛田哲史, 覚道奈津子, 楠本健司
    • Organizer
      The 7th Annual Meeting of the International Federation for Adipose Therapeutics and Science(IFATS)
    • Place of Presentation
      Korea, Daegu, Hotel Inter Burgo
    • Year and Date
      2009-10-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] Possibility of myofibroblast differentiation of human adipose-derived stem cell2009

    • Author(s)
      覚道奈津子, 櫛田哲史, 楠本健司
    • Organizer
      The 7th Annual Meeting of the International Federation for Adipose Therapeutics and Science(IFATS)
    • Place of Presentation
      Korea, Daegu, Hotel Inter Burgo
    • Year and Date
      2009-10-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒト脂肪組織由来幹細胞と増殖因子を用いた組織再生と展望2009

    • Author(s)
      覚道奈津子, 鈴木健司, 櫛田哲史, 楠本健司
    • Organizer
      第18回日本形成外科学会基礎学術集会シンポジウム
    • Place of Presentation
      東京, 都市センターホテル
    • Year and Date
      2009-10-01
    • Related Report
      2009 Final Research Report
  • [Presentation] ヒト脂肪組織由来幹細胞と増殖因子を用いた組織再生と展望2009

    • Author(s)
      覚道奈津子, 櫛田哲史, 楠本健司
    • Organizer
      第18回日本形成外科学会基礎学術集会
    • Place of Presentation
      東京・都市センターホテル
    • Year and Date
      2009-10-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] 線維芽細胞・脂肪組織細胞に対する多血小板血漿および線維芽細胞増殖因子による増殖効果2009

    • Author(s)
      覚道奈津子, 櫛田哲史, 楠本健司
    • Organizer
      第41回日本臨床分子形態学会総会・学術集会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2009-09-04
    • Related Report
      2009 Final Research Report
  • [Presentation] Pletelet-Rich Plasma(多血小板血漿)によるヒト脂肪組織由来幹細胞とヒト線維芽細胞に対する細胞増殖効果2008

    • Author(s)
      覚道奈津子, 南方竜也, 光井俊人, 櫛田哲史, Frederik Zefanya Notodihardjo, 楠本健司
    • Organizer
      第17回日本形成外科学会基礎学術集会
    • Place of Presentation
      東京, リーガロイヤルホテル東京
    • Year and Date
      2008-10-02
    • Related Report
      2009 Final Research Report
  • [Presentation] Proliferation-promoting effect of PRP on human ASCs and DFs2008

    • Author(s)
      Kakudo N, Minakata T, Mitsui T, Kushida S, F.Z. Notodihardjo, Kusumoto K
    • Organizer
      The XIXth Congress of the European Association for Cranio-Maxillofacial Surgery
    • Place of Presentation
      Bologna, Italy, Palazzo dellla Cultura e dei Congressi
    • Year and Date
      2008-09-10
    • Related Report
      2009 Final Research Report
  • [Presentation] Proliferation-promoting effect of PRP on human ASCs and DFs2008

    • Author(s)
      Kakudo N, Minakata T, Mitsui T, Kushida S, F. Z. Notodiharjo, Kusumoto K
    • Organizer
      The XIXth Congress of the European Association for Cranio-Maxillofacial Surgery
    • Place of Presentation
      Bologna/Italy
    • Year and Date
      2008-09-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] ヒト脂肪組織由来幹細胞におけるFGF-2の脂肪分化に与える影響2007

    • Author(s)
      覚道 奈津子、下間 亜由子、楠本 健司
    • Organizer
      第37回日本創傷治癒学会
    • Place of Presentation
      東京
    • Year and Date
      2007-12-07
    • Related Report
      2007 Annual Research Report
  • [Presentation] ヒト脂肪組織由来幹細胞におけるFGF-2の脂肪分化に与える影響2007

    • Author(s)
      覚道奈津子, 下間亜由子, 楠本健司
    • Organizer
      第37回日本創傷治癒学会
    • Place of Presentation
      横浜市, 横浜ロイヤルパークホテル
    • Year and Date
      2007-12-06
    • Related Report
      2009 Final Research Report
  • [Presentation] ヒト脂肪組織由来幹細胞におけるFGF-2の脂肪分化に与える影響2007

    • Author(s)
      覚道奈津子, 下間亜由子, 楠本健司
    • Organizer
      第16回日本形成外科学会基礎学術集会
    • Place of Presentation
      神戸市, クラウンプラザ神戸
    • Year and Date
      2007-10-11
    • Related Report
      2009 Final Research Report
  • [Presentation] PRP(多血小板血漿)とPRS(血小板放出上清)がヒト脂肪由来幹細胞の増殖に及ぼす影響2007

    • Author(s)
      覚道奈津子, 大西早百合, 南方竜也, 楠本健司
    • Organizer
      第16回日本形成外科学会基礎学術集会
    • Place of Presentation
      神戸市, クラウンプラザ神戸
    • Year and Date
      2007-10-11
    • Related Report
      2009 Final Research Report
  • [Presentation] Osteogenic potential of adipose-derived stem cells in vitro and in vivo.2007

    • Author(s)
      Kakudo N, Shimotsuma A, Kusumoto K
    • Organizer
      British Association of Plastic Reconstructive and Aesthetic Surgeons 2007 Summer Scientific Meeting
    • Place of Presentation
      Deauville, France, the Royal Barriere Hotel
    • Year and Date
      2007-07-03
    • Related Report
      2009 Final Research Report

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Published: 2007-03-31   Modified: 2016-04-21  

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