The study of repair mechanism by glial cells in injured brain

• Project/Area Number: 19592086
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Yamagata University
• Principal Investigator: ISEKI Ken Yamagata University, 医学部, 准教授 (70332921)
• Co-Investigator(Kenkyū-buntansha): GOTO Kaoru 山形大学, 医学部, 教授 (30234975)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: グリオーシス / グリア細胞 / 反応性アストロサイト / プロテオグリカン / アストロサイト

Research Abstract

ヘパラン硫酸プロテオグリカンの一種であるアグリン(agrin)が脳損傷部位で発現するか否かを、in situ hybridization や免疫染色を用いて検討した。アグリンmRNA は脳損傷周囲のアストロサイトで7 日目にもっとも強く発現することが判明した。免疫組織学的検討ではアストロサイトと血管周囲に発現しており、免疫電顕を用いることにより脳血管関門を形成するアストロサイトの足突起に発現していた。また、損傷部位で神経誘導物質のFGF-2 やHBGAM と共発現していた。これらのことからアグリンは損傷脳において神経回路の再生や脳血管関門の修復に関与している可能性が示唆された。

Research Products

• [Journal Article] Diacylglycerol kinase zeta isassociated with chromatin, butdissociates from condensed chromatinduring mitotic phase in NIH3T3 cells (2008)
  • Author(s): Hasegawa H, Nakano T, Hozumi Y, TakagiM, Ogino T, Okada M, Iseki K, Kondo H,Watanabe M, Martelli AM, Goto K.
  • Journal Title: Jcell Biochem 105 Pages: 756-765
  • Peer Reviewed
• [Journal Article] Diacylglycerol kinase zeta is associated with chromatin, but dissociates from condensed chromatin during mitotic phase in NIH3T3 cells (2008)
  • Author(s): Hasegawa H, et.al.
  • Journal Title: J Cell Biochem 105 Pages: 756-765
  • Peer Reviewed
• [Journal Article] Lipid messenger, diacylglycerol, and its regulator, diacylglycerol kinase, in cells, organs, and animals : history and perspective (2008)
  • Author(s): Goto K, et.al.
  • Journal Title: Tohoku J Exp Med 214 Pages: 199-212
  • NAID: 10024167082
  • Peer Reviewed
• [Journal Article] Cellular biology andpathophysiology of diacylglycerolkinase family, an enzyme responsible forlipid second messenger metabolism morphological aspects in tissues andorgans (2007)
  • Author(s): Goto K, Hozumi Y, Nakano T, Saino-S S,Kondo H
  • Journal Title: Int. Rev. Cytol 264 Pages: 25-63
  • Peer Reviewed
• [Journal Article] Cellular biology and pathophysiology of diacylglycerol kinase family, an enzyme responsible for lipid second messenger metabolism: morphological aspects in tissues and organs. (2007)
  • Author(s): Goto, et. al.
  • Journal Title: Int Rev Cytol 264 Pages: 25-63
  • Peer Reviewed
• [Presentation] Functional implications ofthe diacylglycerol kinase family in thebrain (2008)
  • Author(s): Kaoru Goto
  • Organizer: 23rd Joint Annual Conference ofBiomedical Sciences
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2008-03-29
• [Presentation] Functional implications of the diacylglycerol kinase family in the brain (2008)
  • Author(s): Kaoru Goto
  • Organizer: The 23rd Joint Annual Conference of Biomedical Sciences 2008
  • Place of Presentation: 台湾
  • Year and Date: 2008-03-29
• [Presentation] Diacylglycerol kinase β accumulates on the perisynaptic site of medium spiny neurons in the striatum (2008)
  • Author(s): Yasukazu Hozumi, Ken Iseki, Kaneyuki Kawamae, Kaoru Goto
  • Organizer: 38th Annual Meeting of Neuroscience
  • Place of Presentation: Washington DC, USA
• [Presentation] Nuclear diacylglycerol kinasezeta quickly disappears in neurons underischemic conditions (2007)
  • Author(s): Goto K
  • Organizer: Japan-Taiwan JointConference on Anatomy and Cell Biology
  • Place of Presentation: Sendai
  • Year and Date: 2007-08-07