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Cell dynamics in transition stage from crown to tooth root formation and effects of growth factors in development of Hertwig epithelial root sheath

Research Project

Project/Area Number 19592128
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionIwate Medical University

Principal Investigator

FUJIWARA Naoki  Iwate Medical University, 歯学部, 講師 (20190100)

Co-Investigator(Kenkyū-buntansha) 鍵谷 忠慶  岩手医科大学, 歯学部, 助教 (30405774)
石関 清人  岩手医科大学, 歯学部, 准教授 (50057775)
大島 勇人  新潟大学, 医歯学系, 教授 (70251824)
原田 英光  岩手医科大学, 歯学部, 教授 (70271210)
Co-Investigator(Renkei-kenkyūsha) HARADA Hidemitsu  岩手医科大学, 歯学部, 教授 (70271210)
OHSHIMA Hayato  新潟大学, 医歯学系, 教授 (70251824)
ISHIZEKI Kiyoto  岩手医科大学, 歯学部, 准教授 (50057775)
KAGIYA Tadayoshi  岩手医科大学, 歯学部, 助教 (30405774)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords歯の発生 / 歯根形成 / ヘルトヴィッヒ上皮鞘 / 器官培養 / EGF / サービカルループ / 上皮成長因子 / GFP / 歯根形戌
Research Abstract

臼歯の歯根形成にはヘルトヴィッヒ上皮鞘(HERS)が重要な役割を果たすことが知られているが、その中で理解が乏しいHERS発達のメカニズムについて今回検討した。我々は2つのオリジナルな器官培養系を用いて上皮成長因子epidermal growth factor、インスリン様成長因子insulin-like growth factor-Iが、それぞれ歯冠形成からHERS形成の移行時期、HERSの伸長・発達に重要な作用を示すことを明らかにした。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (47 results)

All 2009 2008 2007

All Journal Article (16 results) (of which Peer Reviewed: 13 results) Presentation (28 results) Book (3 results)

  • [Journal Article] MAPK mediates Hsp25 signaling in incisor development2009

    • Author(s)
      Lee, MJ, Cai, J, Kwak, SW, Cho, SW, Harada, H, Jung, HS.
    • Journal Title

      Histochem Cell Biol 19(e-Pub)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Egf signaling regulates transition from crown to root formation in the development of mouse molars2008

    • Author(s)
      Fujiwara, N., Akimoto, T., Kagiya, T., Ishizeki, K., Harada, H.
    • Journal Title

      Journal of Experimental Zoology-Molecular and Developmental Evolution, Dec. 17(e-Pub)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms regulating transition from crown to root formation in the development of mouse molars2008

    • Author(s)
      Fujiwara, N., Kagiya, T., Ishizeki, K., Harada, H.
    • Journal Title

      J Oral Bioscience 50

      Pages: 154-159

    • NAID

      10027103936

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The effects of LAMP1 and LAMP3 on M180 amelogenin uptake, localization and amelogenin mRNA induction by amelogenin protein2008

    • Author(s)
      Xu L, Harada H, Taniguchi A.
    • Journal Title

      J. Biochem 144(4)(Epub)

      Pages: 531-537

    • NAID

      10024913012

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] BMP2-induced gene profiling in dental epithelial cell line2008

    • Author(s)
      Miyoshi, K., Nagata, H., Horiguchi, T., Abe, K., Wahyudi, I.A., Baba, Y., Harada H., Noma, T.
    • Journal Title

      J. Med. Invest 55

      Pages: 216-226

    • NAID

      130004465144

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The epithelial- mesenchymal interaction plays a role in the maintenance of the stem cell niche of mouse incisors via Fgf10 and Fgf9 signaling.2008

    • Author(s)
      Yokohama-Tamaki, T., Fujiwara, N., Shibata, S., Wakisaka, S. and Harada, H.
    • Journal Title

      Open Biotechnol J. 2

      Pages: 111-115

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The epithelial-mesenchymal interaction plays a role in the maintenance of the stem cell niche of mouse incisors via Fgf10 and Fgf9 signaling.2008

    • Author(s)
      Yokohama-Tamaki T., et al.
    • Journal Title

      Open Biotechnol J. 2

      Pages: 111-115

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms regulating transition from crown to root formation in the development of mouse molars.2008

    • Author(s)
      Fujiwara N, et al.
    • Journal Title

      J. Oral Biosci. 50

      Pages: 154-159

    • NAID

      10027103936

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Egf prevents formation of Hertwig's epithelial root sheath during developing mouse molar tooth in vitro.2008

    • Author(s)
      Fujiwara, N., et al.
    • Journal Title

      J Exp Zoolog B Mol Dev Evol. Dec17e-Pub

      Pages: 1-9

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The epithelial-mesenchymal interaction plays a role in the maintenance of the stem cell niche of mouse incisors via Fgf10 and Fgf9 signaling2008

    • Author(s)
      Yokohama-Tamaki T., et. al.
    • Journal Title

      Open Biotechnol J. (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 歯冠形成から歯根形成に移行するメカニズム2007

    • Author(s)
      原田英光、藤原尚樹、大島勇人
    • Journal Title

      岩医大歯誌 32

      Pages: 97-104

    • NAID

      110006368250

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of immortalized dental follicle cells for generating periodontal ligament in vivo2007

    • Author(s)
      Yokoi, T., Saito, M., Kiyono, T., Iseki, S., kosaka,K., Nishida, E., Tsubakimoto, T., Harada, H., Eto, K., Noguchi, T. and Teranaka, T.
    • Journal Title

      Cell Tissue Res. 327(2)

      Pages: 301-11

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of BMP-2 on gene expression of enamel matrix proteins s at the dental epithelial cell line2007

    • Author(s)
      Xu, L., Takahashi, R., Harada, H. and Taniguchi, A.
    • Journal Title

      Open Biotechnol J. 1

      Pages: 18-20

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Egf prevents formation of Hertwig's epithelial root sheath during developing mouse molar tooth in vitro2007

    • Author(s)
      Fujiwara, N., Kagiya, T., Ishizeki, K. and Harada, H.
    • Journal Title

      Eur Cell Mat. 14(Proceeding Suppl. 2)

      Pages: 54-54

    • Related Report
      2008 Final Research Report
  • [Journal Article] A role of Wnt5a in continuously growing mice incisors2007

    • Author(s)
      Kagiya, T., Fujiwara, N., Ishizeki, K. Xiao, J. and Harada, H.
    • Journal Title

      Eur Cell Mat. 14, Suppl Proceeding 2 p91

    • Related Report
      2008 Final Research Report
  • [Journal Article] Egf prevents formation of Hertwig's epithelial root sheath during developing mouse molar tooth in vitro.2007

    • Author(s)
      Fujiwara, N., et. al.
    • Journal Title

      Eur Cell Mat.(Proceeding) 14(Suppl.2)

      Pages: 54-54

    • Related Report
      2007 Annual Research Report
  • [Presentation] 歯胚組織中の細胞動態を観察するためのimaging systemの開発2009

    • Author(s)
      藤原尚樹、大津圭史、秋元義、鍵谷忠慶、石関清人、原田英光
    • Organizer
      第114回日本解剖学会学術集会
    • Place of Presentation
      岡山理科大学(岡山)
    • Year and Date
      2009-03-30
    • Related Report
      2008 Final Research Report
  • [Presentation] 歯の再生を目的としたiPS細胞の上皮、間葉細胞への分化誘導と分離培養2009

    • Author(s)
      大津圭史、藤原尚樹、石関清人、鍵谷忠慶、原田英光
    • Organizer
      第114回日本解剖学会学術集会
    • Place of Presentation
      岡山理科大学(岡山)
    • Year and Date
      2009-03-30
    • Related Report
      2008 Final Research Report
  • [Presentation] 歯の再生を目的としたiPS細胞の上皮、間葉細胞への分化誘導と分離培養2009

    • Author(s)
      大津圭史 ら
    • Organizer
      第114回日本解剖学会学術集会
    • Place of Presentation
      岡山
    • Year and Date
      2009-03-30
    • Related Report
      2008 Annual Research Report
  • [Presentation] 歯胚組織中の細胞動態を観察するためのimaging systemの開発2009

    • Author(s)
      藤原尚樹 ら
    • Organizer
      第114回日本解剖学会学術集会
    • Place of Presentation
      岡山
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 歯冠から歯根形成への移行過程におけるEGFsignalingの役割2008

    • Author(s)
      藤原尚樹、大津圭史、秋元義、鍵谷忠慶、石関清人、原田英光
    • Organizer
      第50回歯科基礎医学会学術大会
    • Place of Presentation
      昭和大学(東京)
    • Year and Date
      2008-09-23
    • Related Report
      2008 Final Research Report
  • [Presentation] 歯冠から歯根形成への移行過程におけるEGF signalingの役割2008

    • Author(s)
      藤原尚樹 ら
    • Organizer
      第50回歯科基礎医学会学術大会.
    • Place of Presentation
      東京
    • Year and Date
      2008-09-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] マウス切歯の組織幹細胞を用いた歯の再生.2008

    • Author(s)
      原田 英光, ら
    • Organizer
      第113回日本解剖学会総会・全国学術集会.シンポジウム
    • Place of Presentation
      大分
    • Year and Date
      2008-03-29
    • Related Report
      2007 Annual Research Report
  • [Presentation] ヘルトヴィッヒ上皮鞘細胞の不死化2008

    • Author(s)
      秋元 義, ら
    • Organizer
      第113回日本解剖学会総会・全国学術集会.
    • Place of Presentation
      大分
    • Year and Date
      2008-03-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] マウス切歯の組織幹細胞を用いた歯の再生. シンポジウムS28細胞分化と口腔領域における再生2008

    • Author(s)
      原田英光、藤原尚樹、鍵谷忠慶、石関清人
    • Organizer
      第113回日本解剖学会総会・全国学術集会
    • Place of Presentation
      大分
    • Year and Date
      2008-03-27
    • Related Report
      2008 Final Research Report
  • [Presentation] ヘルトヴィッヒ上皮鞘細胞の不死化2008

    • Author(s)
      秋元義、藤原尚樹、鍵谷忠慶、石関清人、原田英光
    • Organizer
      第113回日本解剖学会総会・全国学術集会
    • Place of Presentation
      大分
    • Year and Date
      2008-03-27
    • Related Report
      2008 Final Research Report
  • [Presentation] マウス切歯の組織幹細胞を用いた歯の再生.2008

    • Author(s)
      原田 英光, ら
    • Organizer
      第7回日本再生医療学会.シンポジウム
    • Place of Presentation
      名古屋
    • Year and Date
      2008-03-14
    • Related Report
      2007 Annual Research Report
  • [Presentation] マウス切歯の組織幹細胞を用いた歯の再生. シンポジウム20歯の再生研究の最前線2008

    • Author(s)
      原田英光、藤原尚樹、鍵谷忠慶、石関清人
    • Organizer
      第7回日本再生医療学会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-03-13
    • Related Report
      2008 Final Research Report
  • [Presentation] Hertwig's epithelial root sheath is made of outer enamel epithelium.2008

    • Author(s)
      Fujiwara, N., et. al.
    • Organizer
      Gordon Research Conference on Craniofacial Morphogenesis & Tissue Regeneration
    • Place of Presentation
      Ciocco,Barga,Italy
    • Year and Date
      2008-02-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] Hertwig's epithelial root sheath is made of outer enamel epithelium2008

    • Author(s)
      Fujiwara, N., Kagiya, T., Ishizeki, K., Otsu, K., Akimoto and T., Harada, H.
    • Organizer
      Gordon Research Conference on Craniofacial Morphogenesis & Tissue Regeneration
    • Place of Presentation
      Ciocco, Barga, Italy
    • Related Report
      2008 Final Research Report
  • [Presentation] 歯の幹細胞の維持機構の解明から歯の再生への展開2007

    • Author(s)
      原田英光、藤原尚樹、鍵谷忠慶、石関清人
    • Organizer
      ワークショップ5W6、歯の発生・再生の分子メカニズム、第30回日本分子生物学会年会と第80回日本生化学会大会合同大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-15
    • Related Report
      2008 Final Research Report
  • [Presentation] 歯の幹細胞の維持機構の解明から歯の再生への展開.2007

    • Author(s)
      原田 英光, ら
    • Organizer
      第30回日本分子生物学会年会と第80回日本生化学会大会合同大会.ワークショップ
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-15
    • Related Report
      2007 Annual Research Report
  • [Presentation] Mechanisms on maintenance of dental stem cells and how to make a biotooth2007

    • Author(s)
      Harada, H., Fujiwara, N., Yokohama-Tamaki, T., Kagiya, T., Tabata, Y. and Ishizeki. K.
    • Organizer
      1^<st> Asian Biomaterials Congress(Integrated Congress of 6^<th> Asian International Symposium on Biomaterials and 8^<th> Asian Symposium on Biomedical Materials)
    • Place of Presentation
      Tsukuba, Japan
    • Year and Date
      2007-12-08
    • Related Report
      2008 Final Research Report
  • [Presentation] Mechanisms on maintenance of dental stem cells and how to make a biotooth.2007

    • Author(s)
      Harada, H., et. al.
    • Organizer
      1^<st> Asian Biomaterials Congress
    • Place of Presentation
      Tsukuba,Ibaraki
    • Year and Date
      2007-12-06
    • Related Report
      2007 Annual Research Report
  • [Presentation] EGF prevent formation of Hertwig's epithelial root sheath during developing mouse molar tooth in vitro2007

    • Author(s)
      Fujiwara, N., Kagiya, T., Ishizeki, K. and Harada, H.
    • Organizer
      9th International Conference on Tooth Morphogenesis and Differentiation 2007
    • Place of Presentation
      Zurich, Switzerland
    • Year and Date
      2007-09-08
    • Related Report
      2008 Final Research Report
  • [Presentation] EGF prevent formation of Hertwig's epithelial root sheath during developing mouse molar tooth in vitro.2007

    • Author(s)
      Fujiwara, N., et. al.
    • Organizer
      9th International Conference on Tooth Morphogenesis and Differentiation 2007.
    • Place of Presentation
      Zurich,Switzerland
    • Year and Date
      2007-09-08
    • Related Report
      2007 Annual Research Report
  • [Presentation] A role of Wnt5a in continuously growing mice incisors.2007

    • Author(s)
      Kagiya, T., et. al.
    • Organizer
      9th International Conference on Tooth Morphogenesis and Differentiation 2007.
    • Place of Presentation
      Zurich,Switzerland
    • Year and Date
      2007-09-08
    • Related Report
      2007 Annual Research Report
  • [Presentation] A role of Wnt5a in continuously growing mice incisors2007

    • Author(s)
      Kagiya, T., Fujiwara, N., Ishizeki, K., Xiao, J. and Harada, H.
    • Organizer
      9th International Conference on Tooth Morphogenesis and Differentiation 2007
    • Place of Presentation
      Zurich, Switzerland
    • Year and Date
      2007-09-06
    • Related Report
      2008 Final Research Report
  • [Presentation] Fgf-9 play a role for the maintenance of stem cell niche via Fgf-10 expression in the mouse incisors2007

    • Author(s)
      Yokohama-Tamaki, T., Shibata, S., Wakisaka, S. and Harada, H.
    • Organizer
      9th International Conference on Tooth Morphogenesis and Differentiation
    • Place of Presentation
      Zurich, Switzerland
    • Year and Date
      2007-09-06
    • Related Report
      2008 Final Research Report
  • [Presentation] 器官培養を用いた歯根発生メカニズムの解明. サテライトシンポジウムSS5-5、ヘルトヴィッヒ上皮鞘(HERS)と歯根発生のバイオロジー2007

    • Author(s)
      藤原尚樹
    • Organizer
      第49回歯科基礎医学会学術大会
    • Place of Presentation
      札幌
    • Year and Date
      2007-08-29
    • Related Report
      2008 Final Research Report
  • [Presentation] ヘルトビッヒの上皮鞘(HERS)形成過程の新規仮説と歯根発生メカニズム. サテライトシンポジウムSS5-5、ヘルトヴィッヒ上皮鞘(HERS)と歯根発生のバイオロジー2007

    • Author(s)
      原田英光, 鍵谷忠慶, 藤原尚樹, 石関清人
    • Organizer
      第49回歯科基礎医学会学術大会
    • Place of Presentation
      札幌
    • Year and Date
      2007-08-29
    • Related Report
      2008 Final Research Report
  • [Presentation] 器官培養を用いた歯根発生メカニズムの解明.2007

    • Author(s)
      藤原 尚樹
    • Organizer
      第49回歯科基礎医学会学術大会.サテライトシンポジウム
    • Place of Presentation
      札幌
    • Year and Date
      2007-08-29
    • Related Report
      2007 Annual Research Report
  • [Presentation] ヘルトビッヒの上皮鞘(HERS)形成過程の新規仮説と歯根発生メカニズム.2007

    • Author(s)
      原田 英光, ら
    • Organizer
      第49回歯科基礎医学会学術大会.サテライトシンポジウム
    • Place of Presentation
      札幌
    • Year and Date
      2007-08-29
    • Related Report
      2007 Annual Research Report
  • [Presentation] 幹細胞を用いた歯科再生研究の現状と臨床への展望と課題2007

    • Author(s)
      原田英光
    • Organizer
      第24回日本顎顔面補綴学会総会
    • Place of Presentation
      盛岡(特別講演)
    • Year and Date
      2007-07-19
    • Related Report
      2008 Final Research Report
  • [Book] 口腔・顔面と歯・唾液腺・舌の発生(口腔内化学, 尾崎登喜雄編集・監修)2008

    • Author(s)
      原田英光
    • Publisher
      飛鳥出版
    • Related Report
      2008 Final Research Report
  • [Book] 器官培養系を用いた歯根形成メカニズムの解明歯界展望(111(5))2008

    • Author(s)
      藤原尚樹
    • Total Pages
      957
    • Publisher
      医歯薬出版
    • Related Report
      2008 Final Research Report
  • [Book] 歯冠形成から歯根形成に移行するメカニズム歯界展望(111(5))2008

    • Author(s)
      原田英光
    • Total Pages
      961
    • Publisher
      医歯薬出版
    • Related Report
      2008 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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