Frontiers in the clarification of the muscular stem cell differentiation process involved in restoring muscle function

• Project/Area Number: 19592131
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: ABE Shin-ichi Tokyo Dental College, 歯学部, 准教授 (40256300)
• Co-Investigator(Kenkyū-buntansha): 崎山 浩司 東京歯科大学, 歯学部, 助教 (50385142) ; 吉成 正雄 東京歯科大学, 歯学部, 准教授 (10085839)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 筋線維 / 筋活性 / 成長因子 / 筋幹細胞 / 筋原線維 / 筋機能 / 筋芽細胞 / 筋収縮タンパク / 筋衛星細胞 / 筋SP細胞 / IGF-1 / 遺伝子導入 / 細胞内ストレス / 筋肥大 / 細胞伸展

Research Abstract

Decreased muscle function is a major cause of the deterioration of eating function including mastication and swallowing in the elderly. We evaluated two processes involved in the mechanism of restoring muscle function. In one process, muscle protein synthesis (promotion) exceeded its degradation (inhibition), and a resulting increase in muscle fiber thickness increases muscle tissue weight, and also improves muscle function. In the other process, muscle-specific stem cells produce new muscle fibers. Thus, the interaction of various factors is important.

Research Products

• [Journal Article] Y. HGF and IGF-1 is present during the developmental process of murine masseter muscle. (2009)
  • Author(s): Abe, S., Nonami, K., Iwanuma, O., Hiroki, E., Yanagisawa, N., Sakiyama, K., Ide
  • Journal Title: J Hard Tissue Biol 18 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Increased expression of decorin during regeneration stage of mdx mouse. (2009)
  • Author(s): Abe, S., Hirose, D., Kado, S., Iwanuma, O., Saka, H., Yanagisawa, N., Ide, Y.
  • Journal Title: Anat Sci Int 84 Pages: 305-311
  • NAID: 10027190914
  • Peer Reviewed
• [Journal Article] Ide, Y. Effect of mechanical stretching on expressions of muscle specific transcription factors MyoD, Myf-5, myogenin and MRF4 in proliferated myoblasts. (2009)
  • Author(s): Abe, S., Rhee, S., Iwanuma, O., Hiroki, E., Yanagisawa, K., Sakiyama, K.
  • Journal Title: Anat Histol Embryol 38 Pages: 305-310
  • Peer Reviewed
• [Journal Article] Expression of myostatin and follistatin in mdx mice, an animal model for muscular dystrophy. (2009)
  • Author(s): Abe, S., Soejima, M., Iwanuma, O., Saka, H., Matsunaga, S., Sakiyama, K., Ide, Y
  • Journal Title: Zool Sci 26 Pages: 315-320
  • NAID: 120002227141
  • Peer Reviewed
• [Journal Article] HGF and IGF-1 is present during the developmental process of murine masseter muscle. (2009)
  • Author(s): 阿部伸一
  • Journal Title: J Hard Tissue Biol 18 Pages: 1-6
  • NAID: 10025927497
  • Peer Reviewed
• [Journal Article] Increased expression of decorin during regeneration stage of mdx mouse. (2009)
  • Author(s): 阿部伸一
  • Journal Title: Anat Sci Int 84 Pages: 305-311
  • NAID: 10027190914
  • Peer Reviewed
• [Journal Article] Effect of mechanical stretching on expressions of muscle specific transcription factors MyoD, Myf-5, myogenin and MRF4 in proliferated myoblasts. (2009)
  • Author(s): 阿部伸一
  • Journal Title: Anat Histol Embryol 38 Pages: 305-310
  • Peer Reviewed
• [Journal Article] Changes in the myosin heavy chain 2a and 2b isoforms of the anterior belly of the digastric muscle before and after weaning in mice. (2008)
  • Author(s): Yoshii, M., Sakiyama, K., Abe, S., Agematsu, H., Mitarashi, S., Tamatsu, Y., Ide, Y.
  • Journal Title: Anat Histol Embryol 37 Pages: 147-152
  • Peer Reviewed
• [Journal Article] Effect of mechanical stretching on caspase and IGF-1 expressions during proliferation process of myoblasts. (2008)
  • Author(s): Iwanuma, O., Abe, S., Hiroki, E., Kado, S., Sakiyama, K., Usami, A., Ide, Y.
  • Journal Title: Zool Sci 25 Pages: 242-247
  • NAID: 120001451411
  • Peer Reviewed
• [Journal Article] Relationship between function of masticatory muscle in mouse and properties of muscle fibers. (2008)
  • Author(s): Abe, S., Hiroki, E., Iwanuma, O., Sakiyama, K., Shirakura, Y., Hirose, D., Shimoo, Y., Suzuki, M., Ikari, Y., Kikuchi, R., Ide, Y., Yoshinari, M.
  • Journal Title: Bull Tokyo Dent Coll 49 Pages: 53-58
  • NAID: 130004868628
  • Peer Reviewed
• [Journal Article] Myofiber properties of mouse mylohyoid muscle in the growth period. (2008)
  • Author(s): Kado, S., Abe, S., Hiroki, E., Iwanuma, O., Sakiyama, K., Kim, H., Ide, Y.
  • Journal Title: Zool Sci 25 Pages: 806-810
  • NAID: 120001828708
  • Peer Reviewed
• [Journal Article] Localization of caspase 12 in masseter muscle of mdx mice during regeneration. (2008)
  • Author(s): Abe, S.
  • Journal Title: Anti-Aging Med 5 Pages: 53-56
  • NAID: 130000128971
  • Peer Reviewed
• [Journal Article] Expression of myosin heavy-chain mRNA in cultured myoblasts induced by centrifugal force. (2008)
  • Author(s): Kurokawa, K., Sakiyama, K., Abe, S., Hiroki, E., Naito, K., Nakajima, K., Takeda, T., Inoue, T., Ide, Y., Ishigami, K.
  • Journal Title: Bull Tokyo Dent Coll 49 Pages: 179-184
  • NAID: 130004471662
  • Peer Reviewed
• [Journal Article] Changes in the myosin heavy chain 2a and 2b isoforms of the anterior belly of the digastric muscle before and after weaning in mice. (2008)
  • Author(s): Yoshii M, Sakiyama K, Abe s., et al. (7名)
  • Journal Title: Anat. Histol. Embryol. 37 Pages: 147-152
  • Peer Reviewed
• [Journal Article] Effect of mechanical stretching on caspase and IGF-1 expressions during proliferation process of myoblasts. (2008)
  • Author(s): Iwanuma O, Abe S., et al. (7名)
  • Journal Title: Zool Sci. 25 Pages: 242-247
  • NAID: 120001451411
  • Peer Reviewed
• [Journal Article] Changes in the muscle fibre properties of the mouse temporal muscle after weaning. (2007)
  • Author(s): Suzuki, K., Abe, S., Kim, H.J., Usami, A., Iwanuma, O., Okubo, H., Ide, Y.
  • Journal Title: nat Histol Embryol 36 Pages: 103-106
  • Peer Reviewed
• [Journal Article] Effects of stretching stimulation with different rates on the expression of MyHC mRNA in mouse cultured myoblasts. (2007)
  • Author(s): Kurokawa, K., Abe, S., Sakiyama, K., Takeda, T., Ide, Y., Ishigami, K.
  • Journal Title: Biomed Res 28 Pages: 25-31
  • NAID: 130004903722
  • Peer Reviewed
• [Journal Article] Activation of caspase 3, 9, 12 and Bax in masseter muscle of mdx mice during necrosis. (2007)
  • Author(s): Honda, A., Abe, S., Hiroki, E., Honda, H., Iwanuma, O., Yanagisawa, N., Ide, Y.
  • Journal Title: J Muscle Res Cell Motil 29 Pages: 243-247
  • Peer Reviewed
• [Journal Article] Activation of caspase 3,9,12 and Bax in masseter muscle of mdx mice during necrosis. (2007)
  • Author(s): Honda A, Abe S, et. al.(7名)
  • Journal Title: J Muscle Res Cell Motil 29 Pages: 243-247
  • Peer Reviewed
• [Journal Article] Changes in the muscle fibre properties of the mouse temporal muscle after weaning. (2007)
  • Author(s): Suzuki K, Abe S, et. al.(7名)
  • Journal Title: Anat.Histol.Embryol. 36 Pages: 103-106
  • Peer Reviewed
• [Journal Article] Effects of stretching stimulation with different rates on the expression of MyHC mRNA in mouse cultured myoblasts. (2007)
  • Author(s): Kurokawa K, Abe S, et. al.(7名)
  • Journal Title: Biomed Res 28 Pages: 25-31
  • NAID: 130004903722
  • Peer Reviewed
• [Presentation] 骨髄由来SP細胞の筋線維への分化について (2010)
  • Author(s): 阿部伸一
  • Organizer: 第115回日本解剖学会全国学術集会
  • Place of Presentation: 岩手県民会館(盛岡市)+
  • Year and Date: 2010-03-28
• [Presentation] 骨髄由来SP細胞の筋線維への分化 (2009)
  • Author(s): 阿部伸一、岩沼治、井出吉信
  • Organizer: 第51回歯科基礎医学会総会
  • Place of Presentation: 新潟市
  • Year and Date: 2009-09-10
• [Presentation] 骨髄由来SP細胞の筋線維への分化について (2009)
  • Author(s): 阿部伸一、監物真、井出吉信、吉成正雄
  • Organizer: 第114回日本解剖学会全国学術集会
  • Place of Presentation: 岡山市
  • Year and Date: 2009-03-30
• [Presentation] 筋線維再活性のための基礎的研究-メカニカルストレスと細胞内ストレス- (2008)
  • Author(s): 阿部伸一, 岩沼治, 崎山浩司, 井出吉信
  • Organizer: 第49回歯科基礎医学会総会
  • Place of Presentation: 昭和大学
  • Year and Date: 2008-09-25
• [Presentation] mdxマウス咬筋の壊死過程におけるCaspaseおよびBaxの活性化 (2008)
  • Author(s): 阿部伸一、廣木愛実、岩沼治、本田敦郎、野波幹三、崎山浩司、井出吉信
  • Organizer: 第113回日本解剖学会全国学術集会
  • Place of Presentation: 大分県由布市
  • Year and Date: 2008-03-29
• [Presentation] 離乳前後におけるマウス顎二腹筋の筋線維特性の変化 (2007)
  • Author(s): 阿部伸一、角祥太郎、中村弘明
  • Organizer: 日本動物学会第78回大会
  • Place of Presentation: 弘前市
  • Year and Date: 2007-09-21
• [Presentation] 筋線維再活性のための基礎的研究-メカニカルストレスと細胞内ストレス- (2007)
  • Author(s): 阿部 伸一, 他(4名)
  • Organizer: 第49回歯科基礎医学会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2007-08-31
• [Presentation] 筋線維再活性のための基礎的研究 (2007)
  • Author(s): 阿部伸一、岩沼治、崎山浩司、井出吉信
  • Organizer: 第49回歯科基礎医学会総会
  • Place of Presentation: 札幌市
  • Year and Date: 2007-08-30