Spatial and temporal analysis of regulatory mechanism by Wnt signaling pathway during dental pulp development.
| Project/Area Number |
19592136
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
SUNOHARA Masataka The Nippon Dental University, 生命歯学部, 准教授 (70287770)
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| Co-Investigator(Kenkyū-buntansha) |
MIYADO Mami 日本歯科大学, 生命歯学部, 助教 (00386252)
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| Research Collaborator |
MURATA Hidetaka 日本歯科大学, 大学院・生命歯学研究科, 大学院生
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 歯学 / 歯髄 / Wntシグナル / 遺伝子発現 |
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| Research Abstract |
Purpose : Wnt signaling has many critical roles in development and also Protein kinase C (PKC) family plays key roles in the signal transduction pathways within the cell. The function of both Wnt and PKC signaling during tooth development remains unknown. Wnt is known to activate some different pathways and PKC is thought to be involved in the Wnt signaling pathway. In this study, we examined both Wnt and PKC signaling pathway involved in tooth germ development. Methods : We stained serial paraffin sections with an antibody against Wnt gene family, beta-catenin and PKC isoforms by using immunohistochemical methods and we performed in situ hybridization by using Wnt genes and beta-catenin as probes. Results : In mouse embryo at days 16.5 post-coitum, we observed Wnt proteins, beta-catenin and PKC isoforms were expressed in tooth germ by immunohistochemical analysis. Beta-catenin was localized in tooth germ and also the localization of beta-catenin transcript was overlapped with that of Wnt genes. Conclusions : Thus this study clearly indicates that Wnt/protein kinase C signaling pathway are involved in tooth germ development in mice. *This study was supported by Grants-in-Aid for Scientific Research (No.19592136).
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Report
(4 results)
Research Products
(10 results)
