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Analysis of the role of cartilage-specific gene in endochondral bone formation using BAC (bacterial artificial chromosome)-transgenic mice

Research Project

Project/Area Number 19592145
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionOkayama University

Principal Investigator

HATTORI Takako  Okayama University, 大学院・医歯薬学総合研究科, 助教 (00228488)

Co-Investigator(Kenkyū-buntansha) TAKIGAWA Masaharu  岡山大学, 大学院・医歯薬学総合研究科, 教授 (20112063)
KUBOTA Satoshi  岡山大学, 大学院・医歯薬学総合研究科, 准教授 (90221936)
NISHIDA Takashi  岡山大学, 大学院・医歯薬学総合研究科, 助教 (30322233)
AOYAMA Eriko  岡山大学, 大学院・医歯薬学総合研究科, 助教 (10432650)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
KeywordsSox9 / Bacterial artificial chromosome(BAC)DNA / トランスジェニックマウストランスジェニックマウス / X型コラーゲンプロモーター / 肥大化軟骨細胞層 / VEGF / 血管侵入 / 内軟骨性骨形成 / bacterial artificial chromosome(BAC)DNA / トランスジェニックマウス / 10型コラーゲンプロモーター / 肥大化軟骨組織 / vegf / 骨髄 / BAC recombination / X型コラーゲン / 肥大軟骨層 / 大腸菌内相同組換え / bacterial artificial chromosome (BAC)
Research Abstract

全長10型コラーゲン遺伝子を含むbacterial artificial chromosome(BAC)DNAの10型コラーゲンプロモーター領域下流にSox9 cDNAを挿入し、Sox9を肥大化軟骨層に異所性に過剰発現するBACトランスジェニックマウスを作製し,(1) 骨髄の消失、肥大化軟骨層への血管侵入の遅延、肥大化軟骨細胞層の延長に起因する骨の短縮、(2) 延長した肥大化軟骨細胞層でのSox9の強い発現に加え、肥大化軟骨マーカー遺伝子の発現の低下、(3) Sox9は直接的にyθgfプロモーター活性を低下させる事、を明らかにし、これらの事からSox9の肥大軟骨細胞層における消失は、血管の侵入、骨髄の形成を可能にし,正常な内軟骨性骨形成に必須である事をin vivoおよびin vivoで明らかにした。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (43 results)

All 2009 2008 2007 Other

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (28 results) Book (1 results) Remarks (1 results)

  • [Journal Article] N terminal domains of CCN protein 2/connective tissue growth factor bind to aggrecan2009

    • Author(s)
      Aovama E, Hattori T, 他5名
    • Journal Title

      Biochem J. in press

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] N-terminal domains of CCN protein 2/connective tissue growth factor bind to aggrecan.2009

    • Author(s)
      Aoyaraa E, et al.
    • Journal Title

      Biochem J. (in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CCN family 2 / Connective tissue growth factor (CCN2/CTGF) stimulates proliferation and differentiation of auricular chondrocytes2008

    • Author(s)
      Fujisawa T., Hattori T. 他5名
    • Journal Title

      Osteoarthritis and Cartilage 16

      Pages: 787-795

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Specific expression of Cre recombinase in hypertrophic cartilage under the control of a BAC CollOal promoter2008

    • Author(s)
      Gebhard S., Hattori T. 他5名
    • Journal Title

      Matrix Biol. 27(8)

      Pages: 693-699

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptional regulation of chondrogenesis by coactivator Tip60 via chromatin association with Sox9 and SoxS2008

    • Author(s)
      Hattori T. 他6名
    • Journal Title

      Nucleic Acids Research 36

      Pages: 3011-3024

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] CCN family 2 / Connective tissue growth factor (CCN2/CTGF)stimulates proliferation and differentiation of auricular chondrocytes.2008

    • Author(s)
      Fujisawa T., et al.
    • Journal Title

      Osteoarthritis and Cartilage 16

      Pages: 787-795

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptional regulation of chondrogenesis by coactivator Tip60 viachromatin association with Sox9 and Sox5.2008

    • Author(s)
      Hattori T., et al.
    • Journal Title

      Nucleic Acids Research 36

      Pages: 3011-3024

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Specific expression of Cre recombinase in hypertrophic cartilage under the control of a BAC-Co110al promoter.2008

    • Author(s)
      Gebhard S., et al.
    • Journal Title

      Matrix Biol. 27

      Pages: 693-699

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] BAC constructs in transgenic reporter mouse lines control efficient and specific LacZ expression in hypertrophic chondrocytes under the complete CollOal promoter2007

    • Author(s)
      Gebhard S., Hattori T. 他6名
    • Journal Title

      Histochem. Cell Biol 127(2)

      Pages: 183-94

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] BAC constructs in transgenic reppter mouse lines control efficient and specific LacZ expression in hypertrophic chondrocytes under the complete Co110al promoter.2007

    • Author(s)
      Schmidl M., et. al.
    • Journal Title

      Histochem. Cell Biol. 127(2)

      Pages: 183-194

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CCN family 2/Connective tissue growth factor(CCN2/CTGF)stimulates proliferation and differentiation of auricular chondrocytes.2007

    • Author(s)
      Fujisawa T., et. al.
    • Journal Title

      Osteoarthritis and Cartilage (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptional regulation of chondrogenesis by coactivator Tip60 viachromatin association with Sox9 and Sox5.2007

    • Author(s)
      Hattori T., et. al.
    • Journal Title

      Nucleic Acids Research (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 結合組織成長因子CTGF/CCN2(Connective tissue growth factor(CTGF)/CCN2)2007

    • Author(s)
      服部高子、他
    • Journal Title

      The Lung perspectives 15(3)

      Pages: 331-335

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] CCN2/CTGF has anti-aging effects to protect articular cartilage from age-rel2009

    • Author(s)
      Shinsuke Itoh, et al.
    • Organizer
      2nd Joint Meeting of the IBMS and the ANZBMS
    • Place of Presentation
      Sydney, Australia
    • Related Report
      2008 Annual Research Report
  • [Presentation] CCN2/CTGFの軟骨特異的過剰発現は加齢に伴う膝関節軟骨の変性に対して抑制的に働く2009

    • Author(s)
      伊藤慎将, 他
    • Organizer
      第22回日本軟骨代謝学会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Annual Research Report
  • [Presentation] 軟骨特異的転写因子Sox9のSUMO化による活性調節機構の解明2008

    • Author(s)
      服部高子
    • Organizer
      第29回岡山歯学会学会賞受賞講演
    • Place of Presentation
      岡山
    • Year and Date
      2008-11-24
    • Related Report
      2008 Annual Research Report
  • [Presentation] CCN2/CTGF is transactivated through its enhancer element by Sox9 in fibroblasts : possible roles in fibrosis2008

    • Author(s)
      T. Hattori 他6名
    • Organizer
      5TH INTERNATIONAL WORKSHOP ON THE FAMILY OF GENES
    • Place of Presentation
      Toronto, Canada
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] Overexpression of CCN2/CTGF in cartilage shows prolonged bone length resulting from stimulation of chondrogenesis, chondrocyte growth, apoptosis, and bone mineralization during endochondral ossification2008

    • Author(s)
      Nao Tomita, et al.
    • Organizer
      30th Annual Meetingof the American Society for Bone and Mineral Research
    • Place of Presentation
      Montreal, Quebec, Canada
    • Related Report
      2008 Annual Research Report
  • [Presentation] The roles of CCN family 2/connective tissue growth factor (CCN2/CTGF)in skeletal development. -Generation and analysis of transgenic miceoverexpressing CCN2/CTGF in cartilage-2008

    • Author(s)
      Takigawa, M., et al.
    • Organizer
      13th World Congress on Advances in Oncology and 11th International Syposiura on Molecular Medicine
    • Place of Presentation
      Hersonissos, Creta, Greece
    • Related Report
      2008 Annual Research Report
  • [Presentation] Collagen X-BAC constructs in transgenic reporter mouse lines controlefficient and specific Cre expression in hypertrophic chondrocytes.2008

    • Author(s)
      Gebhard S., et al.
    • Organizer
      European Workshop on Growth Plate Research
    • Place of Presentation
      Glasgow, Scotland
    • Related Report
      2008 Annual Research Report
  • [Presentation] Roles of CCN2 in skeletal growth and regeneration. -Requirment for bothendochondral and intramembranous bone formation-2008

    • Author(s)
      Takigawa, M., et al.
    • Organizer
      The 5th International Workishop on the CCN Family of Genes
    • Place of Presentation
      Toronto, Canada
    • Related Report
      2008 Annual Research Report
  • [Presentation] CCN2/CTGF軟骨特異的過剰発現が内軟骨性骨形成に及ぼす影響2008

    • Author(s)
      冨田奈緒, 他
    • Organizer
      第二回日本CCNファミリー研究会
    • Place of Presentation
      岡山
    • Related Report
      2008 Annual Research Report
  • [Presentation] ccn2/ctgf遺伝子のSox9による転写活性化機構2008

    • Author(s)
      服部高子, 他
    • Organizer
      第二回日本CCNファミリー研究会
    • Place of Presentation
      岡山
    • Related Report
      2008 Annual Research Report
  • [Presentation] CCN2/CTGFの加齢に伴う膝関節軟骨変性抑制効果2008

    • Author(s)
      伊藤慎将, 他
    • Organizer
      第二回日本CCNファミリー研究会
    • Place of Presentation
      岡山
    • Related Report
      2008 Annual Research Report
  • [Presentation] CCN2/CTGF軟骨特異的過剰発現マウスの作製とその硬組織の解析2008

    • Author(s)
      冨田奈緒, 他
    • Organizer
      日本生化学会中四国支部例会
    • Place of Presentation
      高松
    • Related Report
      2008 Annual Research Report
  • [Presentation] CCN2/CTGF軟骨特異的過剰発現が骨格形成に及ぼす影響2008

    • Author(s)
      冨田奈緒, 他
    • Organizer
      第50回歯科基礎医学会学術大会, 優秀ポスター賞受賞
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report
  • [Presentation] 軟骨成長・分化因子CCN2/CTGFはMatrilin3に結合する事によってマトリクッスのネットワーク形成を促進する2008

    • Author(s)
      荒木大介, 他
    • Organizer
      第50回歯科基礎医学会学術大会
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report
  • [Presentation] CCN2/CTGFの軟骨特異的過剰発現が内軟骨性骨化に及ぼす影響2008

    • Author(s)
      冨田奈緒, 他
    • Organizer
      第26回日本骨代謝学会
    • Place of Presentation
      大阪
    • Related Report
      2008 Annual Research Report
  • [Presentation] CCN2/CTGFの関節軟骨アンチエイジング作用 : 軟骨組織特異的CCN2/CTGF過剰発現マウスを用いた解析2008

    • Author(s)
      伊藤慎将, 他
    • Organizer
      第26回日本骨代謝学会, IBMSトラベルアワード受賞
    • Place of Presentation
      大阪
    • Related Report
      2008 Annual Research Report
  • [Presentation] 軟骨特異的CCN2/CTGF過剰発現は骨延長を誘導する2008

    • Author(s)
      服部高子, 他
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report
  • [Presentation] CCN2/CTGFの軟骨特異的過剰発現マウスモデルの作製と解析2008

    • Author(s)
      冨田奈緒, 他
    • Organizer
      第21回日本軟骨代謝学会
    • Place of Presentation
      京都
    • Related Report
      2007 Annual Research Report
  • [Presentation] 2型コラーゲンプロモーターで誘導したCCN2/CTGF過剰発現トランスジェニックマウスの作製による軟骨分化におけるCCN2/CTGFの役割解析2007

    • Author(s)
      冨田奈緒, 他
    • Organizer
      中四国骨代謝研究会
    • Place of Presentation
      岡山
    • Year and Date
      2007-07-07
    • Related Report
      2007 Annual Research Report
  • [Presentation] 軟骨特異的にCCN2/CTGFを過剰発現したトランスジェニックマウスの作製と軟骨内骨化におけるCCN2/CTGFの役割解析2007

    • Author(s)
      冨田奈緒, 他
    • Organizer
      第一回日本CCNファミリー研究会
    • Place of Presentation
      ピュアリティまきび、岡山
    • Related Report
      2007 Annual Research Report
  • [Presentation] 軟骨細胞におけるCCN2/CTGFの四つのモジュールの異なった役割2007

    • Author(s)
      青山絵理子, 他
    • Organizer
      第一回日本CCNファミリー研究会
    • Place of Presentation
      ピュアリティまきび、岡山
    • Related Report
      2007 Annual Research Report
  • [Presentation] Tip60によるSox9,Sox5のクロマチンを介した軟骨分化の制御2007

    • Author(s)
      服部高子, 他
    • Organizer
      第25回日本骨代謝学会
    • Place of Presentation
      大阪
    • Related Report
      2007 Annual Research Report
  • [Presentation] 結合組織成長因子(CCN2/CTGF)とアグリカンとの結合の生理的意義の解析2007

    • Author(s)
      青山絵理子, 他
    • Organizer
      第25回日本骨代謝学会
    • Place of Presentation
      大阪
    • Related Report
      2007 Annual Research Report
  • [Presentation] CCN2/CTGF過剰発現トランスジェニックマウスの作製によるCCN2/CTGFの内軟骨性骨化における役割解明2007

    • Author(s)
      冨田奈緒, 他
    • Organizer
      第25回日本骨代謝学会
    • Place of Presentation
      大阪
    • Related Report
      2007 Annual Research Report
  • [Presentation] 軟骨特異的CCN2/CTGF過剰発現トランスジェニックマウスの作製とCCN2/CTGFの内軟骨性骨化に及ぼす影響について2007

    • Author(s)
      冨田奈緒, 他
    • Organizer
      第49回歯科基礎医学会
    • Place of Presentation
      札幌
    • Related Report
      2007 Annual Research Report
  • [Presentation] 軟骨成長・分化因子CCN2/CTGFとMatrilin3の相互作用2007

    • Author(s)
      荒木大介, 他
    • Organizer
      第49回歯科基礎医学会
    • Place of Presentation
      札幌
    • Related Report
      2007 Annual Research Report
  • [Presentation] 軟骨特異的にCCN2/CTGFを過剰発現したトランスジェニックマウスの作製と解析2007

    • Author(s)
      冨田奈緒, 他
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] 結合組織成長因子(CCN2/CTGF)とアグリカンとの結合についての解析2007

    • Author(s)
      青山絵理子, 他
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report
  • [Book] 結合組織成長因子CTGF/CCN2(Connective tissue growth factor(CTGF)/CCN2)The Lung perspectives(15(3))2007

    • Author(s)
      服部高王、久保田聡、滝川正春
    • Publisher
      メディカルレビュー社、大阪
    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.dent.okayama-u.ac.jp/seika/index_sc_j.html

    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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