Functional analyses of the bHLH transcription factor DEC2 in cardiac and neural differentiations.

• Project/Area Number: 19592147
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Hiroshima University
• Principal Investigator: FUJIMOTO Katsumi Hiroshima University, 大学院・医歯薬学総合研究科, 助教 (40294566)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 口腔生化学 / bHLH型転写因子 / 心筋分化 / 神経分化

Research Abstract

We studied about the molecular mechanisms by which Dec2 regulates gene expression. Gel retardation and luciferase assays showed that Dec2 preferentially binds to class B E-box elements (CACGTG) as a homodimer and represses the transcription of target genes in a histone deacetylase (HDAC)-dependent manner. In contrast, Dec2 repressed a MyoD-activated promoter activity of muscle creatine kinase gene through class A E-box in an HDAC1-independent manner. These findings indicate that Dec2 employs multiple mechanisms -including DNA-binding and protein-protein interactions-to achieve E-box-dependent transcriptional repressions.

Research Products

• [Journal Article] Anti-apoptotic effect of the basic helix-loop-helix (bHLH) transcription factor DEC2 in human breast cancer cells (2010)
  • Author(s): Yang Liu, et al.
  • Journal Title: Genes to cells 15・4 Pages: 315-325
  • Peer Reviewed
• [Journal Article] Interleukin-4 up-regulates histamine H1 receptors by activation of H1 receptor gene transcription (2010)
  • Author(s): Shuhei Horio
  • Journal Title: Naunyn-Schmiedeberg's Archives of Pharmacology (In press)
  • Peer Reviewed
• [Journal Article] he Basic Helix-loop-helix Proteins DEC1 and DEC2 Function as Corepressors of Retinoid X Receptors. (2009)
  • Author(s): Cho, Y., Noshiro, M., Choi, M., Morita, K., Kawamoto, T., Fujimoto, K., Kato, Y., Makishima, M.
  • Journal Title: Mol. Pharmacol. 76(6) Pages: 1360-1369
  • Peer Reviewed
• [Journal Article] Liver X receptors(LXRalpha and LXRbeta) are potent regulators for hepatic Dec1 expression. (2009)
  • Author(s): Noshiro, M., Usui, E., Kawamoto, T., Sato, F., Nakashima, A., Ueshima, T., Honda, K., Fujimoto, K., Honma, S., Honma, K., Makishima, M., Kato, Y.
  • Journal Title: Genes Cells 14(1) Pages: 29-40
  • Peer Reviewed
• [Journal Article] The Basic Helix-Loop-Helix Proteins Differentiated Embryo Chondrocyte (DEC) 1 and DEC2 Function as Corepressors of Retinoid X Receptor s (2009)
  • Author(s): Yoshitake Cho, et al.
  • Journal Title: Molecular Pharmacology 76・6 Pages: 1360-1369
  • Peer Reviewed
• [Journal Article] Liver X receptors (LXRalpha and LXRbeta) are potent regulators for hepatic Decl expression. (2009)
  • Author(s): Mitsuhide Noshiro, et al.
  • Journal Title: Genes to Cells 14 Pages: 29-40
  • Peer Reviewed
• [Journal Article] Period is involved in the proliferation of human pancreatic MIA-PaCa2 cancer cells by TNF-alpha. (2008)
  • Author(s): Suzuki, T., Sato, F., Kondo, J., Liu, Y., Kusumi, T., Fujimoto, K., Kato, Y., Sato, T., Kijima, H.
  • Journal Title: Biomed. Res. 29(2) Pages: 99-103
  • NAID: 130004724055
  • Peer Reviewed
• [Journal Article] DEC1 modulates the circadian phase of clock gene expression. (2008)
  • Author(s): Nakashima, A., Kawamoto, T., Honda, K.K., Ueshima, T., Noshiro, M., Iwata, T., Fujimoto, K., Kubo, H., Honma, S., Yorioka, N., Kohno, N., Kato, Y.
  • Journal Title: Mol. Cell. Biol. 28(12) Pages: 4080-4092
  • Peer Reviewed
• [Journal Article] Basic-helix-loop-helix (bHLH) transcription factor DEC2 negatively regulates vascular endothelial growth factor expression. (2008)
  • Author(s): Sato, F., Bhawal, U.K., Kawamoto, T., Fujimoto, K., Imaizumi, T., Imanaka, T., Kondo, J., Koyanagi, S., Noshiro, M., Yoshida, H., Kusumi, T., Kato, Y., Kijima, H.
  • Journal Title: Genes Cells 13(2) Pages: 131-144
  • Peer Reviewed
• [Journal Article] Down-regulation of histamine H1 receptors by beta2-adrenoceptor-mediated inhibition of H1 receptor gene transcription. (2008)
  • Author(s): Miyoshi, K., Kawakami, N., Umehara, H., Fujimoto, K., Horio, S., Fukui, H.
  • Journal Title: J. Pharm. Pharmacol. 60(6) Pages: 747-752
  • Peer Reviewed
• [Journal Article] DEC1 modulates the circadian phase of clock gene expression. (2008)
  • Author(s): Ayumu Nakashima, et al.
  • Journal Title: Molecular and cellular Biology 28 Pages: 4080-4092
  • Peer Reviewed
• [Journal Article] Down-regulation of histamine H1 receptors by beta2-adrenoceptor-mediated inhibition of H1 receptor gene transcription. (2008)
  • Author(s): Katsuhiro Miyoshi, et al.
  • Journal Title: Journal of Pharmacy and Pharmacology 60 Pages: 747-752
  • Peer Reviewed
• [Journal Article] Period is involved in the proliferation of human pancreatic MIA-PaCa2 cancer cells by TNF-alpha. (2008)
  • Author(s): Takahiro Suzuki, et al.
  • Journal Title: Biomedical Research 29 Pages: 99-103
  • Peer Reviewed
• [Journal Article] Basic-helix-loop-helix (bHLH) transcription factor DEC2 negatively ragulates vascular endothelial growth factor expression. (2008)
  • Author(s): Fuyuki Sato
  • Journal Title: Genes to Cells 13 Pages: 131-144
  • Peer Reviewed
• [Journal Article] Multiple mechanisms regulate circadian expression of the gene for cholesterol 7alpha-hydroxylase (Cyp7a), a key enzyme in hepatic bile acid biosynthesis. (2007)
  • Author(s): Noshiro, M., Usui, E., Kawamoto, T., Kubo, H., Fujimoto, K., Furukawa, M., Honma, S., Makishima, M., Honma, K., Kato, Y.
  • Journal Title: J. Biol. Rhythms 22(4) Pages: 299-311
  • Peer Reviewed
• [Journal Article] Transcriptional repression by the basic helix-loop-helix protein Dec2: multiple mechanisms through E-box elements. (2007)
  • Author(s): Fujimoto, K., Hamaguchi, H., Hashiba, T., Nakamura, T., Kawamoto, T., Sato, F., Noshiro, M., Bhawal, U.K., Suardita, K., Kato, Y.
  • Journal Title: Int. J. Mol. Med. 19(6) Pages: 925-932
  • Peer Reviewed
• [Journal Article] Glyceraldehyde-3-phosphate dehydrogenase enhances transcriptional activity of androgen receptor in prostate cancer cells. (2007)
  • Author(s): Harada, N., Yasunaga, R., Higashimura, Y., Yamaji, R., Fujimoto, K., Moss, J., Inui, H., Nakano, Y.
  • Journal Title: J. Biol. Chem. 282(31) Pages: 22651-22661
  • Peer Reviewed
• [Journal Article] Stimulation of histamine H1 receptor up-regulates histamine H1 receptor itself through activation of receptor gene transcription. (2007)
  • Author(s): Das, A.K., Yoshimura, S., Mishima, R., Fujimoto, K., Mizuguchi, H., Dev, S., Wakayama, Y., Kitamura, Y., Horio, S., Takeda, N., Fukui, H.
  • Journal Title: J. Pharmacol. Sci. 103(4) Pages: 374-382
  • Peer Reviewed
• [Journal Article] Heterologous up-regulation of the histamine H1 receptor by M3 muscarinic receptor-mediated activation of H1-receptor gene transcription. (2007)
  • Author(s): Miyoshi, K., Kawakami, N., Das, A.K., Fujimoto, K., Horio, S., Fukui, H.
  • Journal Title: J. Pharm. Pharmacol. 59(6) Pages: 843-848
  • Peer Reviewed
• [Journal Article] Transcriptional repression by the basic helix-loop-helix protein Dec2: multiple mechanisms through E-box elements. (2007)
  • Author(s): Katsumi Fujimoto
  • Journal Title: International Journal Of Molecular Medicine 19 Pages: 925-932
  • Peer Reviewed
• [Journal Article] Multiple mechanisms regulate circadian expression of the gene for cholesterol 7alpha-hydroxylase (Cyp7a), a key enzyme in hepatic bile acid biosynthesis. (2007)
  • Author(s): Mitsuhide Noshiro
  • Journal Title: Journal of Biological Rhythms 22 Pages: 299-311
  • Peer Reviewed
• [Journal Article] Glyceraldehyde-3-phosphate dehydrogenase enhances transcriptional activity of androgen receptor in prostate cancer cells. (2007)
  • Author(s): Naoki Harada
  • Journal Title: Journal of Biological Chemistry 282 Pages: 22651-22661
  • Peer Reviewed
• [Journal Article] Heterologous up-regulation of the histamine H1 receptor by M3 muscarinic receptor-mediated activation of H1-receptor gene transcription. (2007)
  • Author(s): Katsuhiro Miyoshi
  • Journal Title: Journal of Pharmacy and Pharmacology 59 Pages: 843-848
  • Peer Reviewed
• [Journal Article] Stimulation of histamine H1 receptor up-regulates histamine H1 receptor itself through activation of receptor gene transcription. (2007)
  • Author(s): Asish K. Das
  • Journal Title: Journal of Pharmacological Sciences 103 Pages: 374-382
  • Peer Reviewed
• [Presentation] 核受容体による時計遺伝子発現調節機構の解明 (2009)
  • Author(s): 尾崎徳継, 能城光秀, 本田清昌, 河本健, 藤本勝巳, 谷本幸太郎, 加藤幸夫, 丹根一夫
  • Organizer: 第42回広島大学歯学会総会
  • Place of Presentation: 広島市
  • Year and Date: 2009-06-20
• [Presentation] 核受容体による時計遺伝子発現調節機構の解明 (2009)
  • Author(s): 尾崎徳継
  • Organizer: 第42回広島大学歯学会総会
  • Place of Presentation: 広島大学(広島市)
  • Year and Date: 2009-06-20
• [Presentation] 骨髄由来間葉系幹細胞の骨, 脂肪, 軟骨細胞分化におけるDEC2の役割 (2009)
  • Author(s): 笹本智子, 藤本勝巳, 金輪真佐美, 河本健, 能城光秀, 道田将彦, 尾崎徳継, 丹根一夫, 加藤幸夫
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市
• [Presentation] 骨髄由来間葉系幹細胞の骨、脂肪、軟骨細胞分化におけるDEC2の役割 (2009)
  • Author(s): 笹本智子
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
• [Presentation] bHLH型転写因子DECは概日リズムの位相調節に関与する (2008)
  • Author(s): 河本健
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド神戸市
  • Year and Date: 2008-12-12
• [Presentation] DECは核内受容体のコリプレッサーとして機能する (2008)
  • Author(s): 長克武
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド神戸市
  • Year and Date: 2008-12-09
• [Presentation] bHLH型転写因子DEC1は概日リズム遺伝子の位相を調節する (2008)
  • Author(s): 河本健, 中島歩, 本田清昌, 上島太一, 藤本勝巳, 能城光秀, 加藤幸夫
  • Organizer: 第18回中国・四国骨代謝研究会
  • Place of Presentation: 岡山市
  • Year and Date: 2008-07-12
• [Presentation] bHLH型転写因子DEC1概日リズム遺伝子の位相を調節する (2008)
  • Author(s): 河本健
  • Organizer: 第18回中国・四国骨代謝研究会
  • Place of Presentation: 岡山市
  • Year and Date: 2008-07-12
• [Presentation] 軟骨細胞分化の概日リズムを制御する新規時計制御配列の探索と、そこに関与する転写因子の同定 (2008)
  • Author(s): 上嶋 太一
  • Organizer: 第21回日本軟骨代謝学会
  • Place of Presentation: 京都テルサ、京都市
  • Year and Date: 2008-03-22
• [Presentation] 明暗条件は永久軟骨の成長・代謝を制御する (2008)
  • Author(s): 本田 清昌
  • Organizer: 第21回日本軟骨代謝学会
  • Place of Presentation: 京都テルサ,京都市
  • Year and Date: 2008-03-21
• [Presentation] ラット成長版軟骨における核内受容体の発現様式と機能の解析 (2008)
  • Author(s): 尾崎 徳継
  • Organizer: 第21回日本軟骨代謝学会
  • Place of Presentation: 京都テルサ,京都市
  • Year and Date: 2008-03-21
• [Presentation] bHLH型転写因子DECは概日リズムの位相調節に関与する (2008)
  • Author(s): 河本健, 中島歩, 本田清昌, 上嶋太一, 能城光秀, 藤本勝巳, 本間さと, 加藤幸夫
  • Organizer: 第31回日本分子生物学会年会, 第81回日本生化学会合同大会
  • Place of Presentation: 神戸市
• [Presentation] DECは核内受容体のコリプレッサーとして機能する (2008)
  • Author(s): 長克武, 能城光秀, 加藤幸夫, 河本健, 藤本勝巳, 槇島誠
  • Organizer: 第31回日本分子生物学会年会, 第81回日本生化学会合同大会
  • Place of Presentation: 神戸市
• [Presentation] HLH型転写因子DEC1ノックアウトマウスにおける行動および遺伝子発現の概日リズム解析 (2008)
  • Author(s): 河本健, 中島歩, 本田清昌, 上嶋太一, 藤本勝巳, 加藤幸夫
  • Organizer: 第26回日本骨代謝学会学術集会
  • Place of Presentation: 大阪市
• [Presentation] ラット成長板軟骨のおける核内受容体の発現様式と機能の解析 (2008)
  • Author(s): 尾崎徳継, 能城光秀, 本田清昌, 林原久美子, 河本健, 藤本勝巳, 丹根一夫, 加藤幸夫
  • Organizer: 第21回日本軟骨代謝学会
  • Place of Presentation: 京都市
• [Presentation] 軟骨細胞分化の概日リズムを制御する新規時間制御配列の探索と、そこに関与する転写因子の同定 (2008)
  • Author(s): 上嶋太一, 河本健, 本田清昌, 能城光秀, 藤本勝巳, 後藤修, 加藤幸夫
  • Organizer: 第21回日本軟骨代謝学会
  • Place of Presentation: 京都市
• [Presentation] 明暗条件は永久軟骨の成長・代謝を制御する (2008)
  • Author(s): 本田清昌, 河本健, 中島歩, 上嶋太一, 藤本勝巳, 西村正宏, 能城光秀, 加藤幸夫
  • Organizer: 第21回日本軟骨代謝学会
  • Place of Presentation: 京都市
• [Presentation] bHLH型転写因子DEC1ノックアウトマウスにおける行動および遺伝子発現の概日リズム解析 (2008)
  • Author(s): 河本健
  • Organizer: 第26回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場、大阪市
• [Presentation] 前立腺癌細胞においてグリセルアルデヒド-3-リン酸デヒドロゲナーゼはアンドロゲン受容体の転写活性を促進する (2007)
  • Author(s): 原田 直樹
  • Organizer: 第30回日本分子生物学会年会第80回日本生化学会大会 合同大会
  • Place of Presentation: パシフィコ横浜、横浜市
  • Year and Date: 2007-12-12
• [Presentation] ヒトヒスタミンH1受容体遺伝子発現調節機構の解明 (2007)
  • Author(s): 水口 博之
  • Organizer: 第30回日本分子生物学会年会第80回日本生化学会大会 合同大会
  • Place of Presentation: パシフィコ横浜,横浜市
  • Year and Date: 2007-12-12
• [Presentation] DEC1による概日リズム調節の機構 (2007)
  • Author(s): 河本 健
  • Organizer: 第49回歯科基礎医学会
  • Place of Presentation: 北海道大学、札幌市
  • Year and Date: 2007-08-30
• [Presentation] 軟骨細胞分化の概日リズムを制御する新規配列の探索とそこに関与する転写因子の同定 (2007)
  • Author(s): 上嶋太一, 河本健, 本田清昌, 能城光秀, 藤本勝巳, 後藤修, 加藤幸夫
  • Organizer: 中四国骨代謝研究会
  • Place of Presentation: 岡山市
  • Year and Date: 2007-07-07
• [Presentation] 軟骨細胞分化の概日リズムを制御する新規配列の探索とそこに関与する転写因子の同定 (2007)
  • Author(s): 上嶋 太一
  • Organizer: 中四国骨代謝研究会
  • Place of Presentation: 岡山
  • Year and Date: 2007-07-07
• [Presentation] The liver X receptors (LXRa and LXRb) are potent regulators for hepatic Dec1 expression (2007)
  • Author(s): N oshiro M., Usui E., Kawamoto T., Sato F., Nakashima A., Furukawa M., Ueshima T., Honda K., Fujimoto K., Honma S., Honma K., Kato Y.
  • Organizer: 2nd World Congress of Chronobiology
  • Place of Presentation: 東京都
• [Presentation] DEC1 modulates circadian phase of clock gene expression (2007)
  • Author(s): N akashima A., Kawamoto T., Honda K., Ueshima T., Noshiro M., Fujimoto K., Kubo H., Kato Y.
  • Organizer: 2nd World Congress of Chronobiology
  • Place of Presentation: 東京都
• [Presentation] DEC1による概日リズム調節の機構 (2007)
  • Author(s): 河本健, 本田清昌, 藤本勝巳, 能城光秀, 加藤幸夫
  • Organizer: 第49回歯科基礎医学会
  • Place of Presentation: 札幌市
• [Presentation] 軟骨細胞の分化の概日リズムを制御する数種類の新規制御配列の同定とそこに関与する転写因子の探索 (2007)
  • Author(s): 上嶋太一, 河本健, 能城光秀, 藤本勝巳, 本田清昌, 中島歩, 加藤幸夫
  • Organizer: 第25回日本骨代謝学会学術集会
  • Place of Presentation: 大阪市
• [Presentation] The liver X receptors (LXRa and LXRb) are potent regulators for hepatic Dec1 expression (2007)
  • Author(s): Mitsuhide Noshiro
  • Organizer: 2nd World Congress of Chronobiology
  • Place of Presentation: 京王プラザホテル,東京都
• [Presentation] DEC1 modulates circadian phase of clock gene expression (2007)
  • Author(s): Ayumu Nakashima
  • Organizer: 2nd World Congress of Chronobiology
  • Place of Presentation: 京王プラザホテル,東京都
• [Presentation] 軟骨細胞の分化の概日リズムを制御する数種類の新規制御配列の同定とそこに関与する転写因子の探索 (2007)
  • Author(s): 上嶋 太一
  • Organizer: 第25回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場、大阪市