Basic studies for drug discovery using adhesin domain protein and interdomain regional peptide of Porphyromonas gingivalis

• Project/Area Number: 19592151
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Nagasaki University
• Principal Investigator: SAKAI Eiko Nagasaki University, 医歯薬学総合研究科, 助教 (10176612)
• Co-Investigator(Kenkyū-buntansha): 中山 浩次 長崎大学, 医歯薬学総合研究科, 教授 (80150473) ; 岡元 邦彰 長崎大学, 医歯薬学総合研究科, 准教授 (10311846) ; 内藤 真理子 長崎大学, 医歯薬学総合研究科, 准教授 (20244072)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 歯科薬理学 / 歯学 / 薬理学 / 細菌 / タンパク質 / 糖鎖 / 蛋白質

Research Abstract

歯周病原細菌Porphyromonas gingivalisのrgp A遺伝子産物であるHGP44のN末端アミノ酸を欠失させた変異蛋白を作成し、赤血球凝集活性を調べた。N末端20アミノ酸を欠失すると血球凝集活性が消失したため、この領域が活性中心である可能性が考えられた。シアル酸のはずれた糖蛋白と結合しやすく、また赤血球凝集の強さが動物種によって異なることからGalBl-4GlcNAcの糖鎖を介して糖蛋白と結合している可能性が示唆された。

Research Products

• [Journal Article] Determination of active site of lysine-specific cysteine proteinase (Lys-gingipain) by use of a Porphyromonas gingivalis plasmid system (2008)
  • Author(s): Ishida Y., Hu J. P., Sakai E., Kadowaki T., Yamamoto K., Tsukuba T., Kato Y., Nakayama K., Okamoto K
  • Journal Title: Arch Oral Biol 53(6) 53(6) Pages: 538-544
  • NAID: 120006984583
  • Peer Reviewed
• [Journal Article] Construction of recombinant hemagglutinin derived from the gingipain-encoding gene of Porphyromonasgingivalis, identification of its target protein on erythrocytes, and inhibition of hemagglutination by an interdomain regional peptide (2007)
  • Author(s): Sakai E., Naito M. , Sato K. , Hotokezaka H., Kadowaki T., Kamaguchi A., Yamamoto K., Okamoto K., Nakayama K
  • Journal Title: J. Bacteriol. 189(11) 189(11) Pages: 3977-3986
  • Peer Reviewed
• [Journal Article] Construction of Recombinant Hemagglutinin Derived from the Gingipain-Encoding Gene of Porphyromonas gingivalis, Identification of Its Target Protein on Erythrocytes, and Inhibition of Hemagglutination by an Interdomain Regional Peptide (2007)
  • Author(s): Eiko Sakai
  • Journal Title: Journal of Bacteriology 189 Pages: 3977-3986
  • Peer Reviewed
• [Presentation] 歯周病細菌Porphyrpmonas ginivalisの赤血球凝集機構の解析 (2007)
  • Author(s): 中山浩次
  • Organizer: 第39回九州微生物研究会
  • Place of Presentation: 福岡