Identification of SNARE proteins for salivary exocytosis
| Project/Area Number |
19592153
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
TAKUMA Taishin Health Sciences University of Hokkaido, 歯学部, 教授 (40095336)
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| Co-Investigator(Kenkyū-buntansha) |
ARAKAWA Toshiya 北海道医療大学, 歯学部, 講師 (40306254)
SITARA Akiko 北海道医療大学, 歯学部, 助教 (30508718)
OKAYAMA Miki 北海道医療大学, 歯学部, 助教 (30382500)
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| Project Period (FY) |
2007 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 口腔生化学 / TIRF / 開口分泌 / 唾液腺 / SNARE / siRNA / GFP |
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| Research Abstract |
本研究では、当初、仙台ウイルス(HVJ)・エンベロープ・ベクターを用い、分泌マーカーcDNAと分泌関連タンパク質のsiRNAをラット顎下腺に導入し、唾液分泌機構のin vivo解析を計画した。しかし、予期に反し、このエンベロープ・ベクターには遺伝子導入活性がなかったため、計画変更を迫られた。そこで、培養細胞に、分泌マーカーとSNAREタンパク質を別の色の蛍光タンパク質として発現し、二波長全反射蛍光顕微鏡観察法により、構成的分泌を調節する分泌関連タンパク質の同定を試みており、現在、siRNAの効果から対象が徐々にしぼられてきている。
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Report
(3 results)
Research Products
(18 results)
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[Journal Article] High frequency of hypermethylation of p14, p15 and p16 in oral pre-cancerous lesions associated with betel-quid chewing in Sri Lanka2008
Author(s)
Takeshima M, Saitoh M, Kusano K, Nagayasu H, Kurashige Y, Malsantha M, Arakawa T, Takuma T, Chiba I, Kaku T, Shibata T, Abiko Y
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Journal Title
J Oral Pathol Med 37(8)
Pages: 475-479
Related Report
Peer Reviewed
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[Journal Article] High frequency of hypermethylation of p14, p15 and p16 in oral pre-cancerous lesions associated with betel-quid chewing in Sri Lanka.2008
Author(s)
Takeshima M, Saitoh M, Kusano K, Nagayasu H, Kurashige Y, Malsantha M, Arakawa T, Takuma T, Chiba I, Kaku T, Shibata T, Abiko Y.
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Journal Title
J Oral Pathol Med. 37
Pages: 475-479
Related Report
Peer Reviewed
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[Journal Article] Increased expression of beta-defensin-2 and -3 during the development of autoimmune sialoadenitis in MRL/lpr mice2007
Author(s)
Saitoh M, Kurashige Y, Yamazaki M, Nishimura M, Nakamura S, Noro D, Takeshima M, Arakawa T, Takuma T, Igarashi S, Kaku T, Inoue T, Abiko Y
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Journal Title
Med Mol Morphol 40(3)
Pages: 157-162
Related Report
Peer Reviewed
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