| Project/Area Number |
19592157
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Meikai University |
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Principal Investigator |
NAKANISHI Nobuo Meikai University, 歯学部, 講師 (20118574)
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| Co-Investigator(Kenkyū-buntansha) |
KURIHARA Kinji 明海大学, 歯学部, 講師 (10170086)
WATABE Shoji 山口大学, 医学系研究科, 教授 (30113020)
HASEGAWA Hiroyuki 帝京科学大学, 理工学部, 教授 (10092983)
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| Project Period (FY) |
2007 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 内在性アンチセンスRNA / GTPシクロヒドロラーゼ / 遺伝子発現調節 / テトラヒドロビオプテリン / 一酸化窒素 / 神経伝達物質 / トランスクリプトーム / natural antisense RNA / GTP cyclohydrolase 1 / tetrahydrobiopterin / nitric oxide synthase / RNA interference / transcriptome / microRNA / post-transcriptional regulation / アンチセンスRNA / RNA干渉 / 転写 / transcriptional regulation / GTP cyclohydrolase I / RNA editing |
|---|
| Research Abstract |
Tetrahydrobiopterin (BH4) is an essential cofactor for the enzymes which synthesize monoamine neurotransmitters and nitric oxide, thus influencing neuronal and vascular functions. We found in PC12 cells and in rat and mouse livers natural antisense RNA (naRNA) of GTP cyclohydrolase1 (GCH), a rate-limiting enzyme in BH4 de novo synthesis. When the PC12 cells were stimulated by various agents which increased the cellular BH4 level, GCH naRNA was increased in parallel with GCH mRNA.
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