Establishment of patho-physiological diagnosis and rational treatment protocol for apical periodontitis using qualitative and quantitative analysis of intracanal microflora.
| Project/Area Number |
19592193
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Conservative dentistry
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
YAMAKI Keiko Tohoku University, 大学院・歯学研究科, 助教 (90182419)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SHIMAUCHI Hidetoshi 東北大学, 大学院・歯学研究科, 教授 (70187425)
SATO Takuichi 東北大学, 大学院・歯学研究科, 講師 (10303132)
|
|---|
| Project Period (FY) |
2007 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Keywords | 歯内療法学 / 根尖性歯周炎 / 嫌気性菌 / 16S rRNA / PCR / 16S ribosomal RNA / 根管内滲出液 |
|---|
| Research Abstract |
Changes of intracanal microflora during the course of root canal therapy were investigated in patients with apical peridontitis. Informed consent was obtained prior to dentin sample collection. Anaerobic culture of the samples followed by molecular genetic analysis revealed that : a marked variation of the microbial composition was found among the cases according to their clinical conditions such as history of treatment and accessibility to oral cavity ; Pseudoramibacter, Olsenella, Propionibacterium, Lactobacillus were the frequently-detected species; the conventional treatment protocol was usually capable of maneuvering intracanal infection regardless of bacterial species or bacterial loads.
|
Report
(4 results)
Research Products
(23 results)
