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Establishment of patho-physiological diagnosis and rational treatment protocol for apical periodontitis using qualitative and quantitative analysis of intracanal microflora.

Research Project

Project/Area Number 19592193
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionTohoku University

Principal Investigator

YAMAKI Keiko  Tohoku University, 大学院・歯学研究科, 助教 (90182419)

Co-Investigator(Kenkyū-buntansha) SHIMAUCHI Hidetoshi  東北大学, 大学院・歯学研究科, 教授 (70187425)
SATO Takuichi  東北大学, 大学院・歯学研究科, 講師 (10303132)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords歯内療法学 / 根尖性歯周炎 / 嫌気性菌 / 16S rRNA / PCR / 16S ribosomal RNA / 根管内滲出液
Research Abstract

Changes of intracanal microflora during the course of root canal therapy were investigated in patients with apical peridontitis. Informed consent was obtained prior to dentin sample collection. Anaerobic culture of the samples followed by molecular genetic analysis revealed that : a marked variation of the microbial composition was found among the cases according to their clinical conditions such as history of treatment and accessibility to oral cavity ; Pseudoramibacter, Olsenella, Propionibacterium, Lactobacillus were the frequently-detected species; the conventional treatment protocol was usually capable of maneuvering intracanal infection regardless of bacterial species or bacterial loads.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (23 results)

All 2010 2009 2007

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (11 results) Book (3 results)

  • [Journal Article] Profiling of subgingival plaque biofilm microflora from periodontally healthy subjects and from subjects with periodontitis using quantitative real-time PCR2010

    • Author(s)
      Abiko Y, Sato T, Mayanagi G, Takahashi N
    • Journal Title

      J Periodont Res 45

      Pages: 389-395

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Host β-globin gene fragments in crevivular fluid as a biomarker in periodontal health and disease2010

    • Author(s)
      Thaweboon B, Laohapand P, Amornchat C, Matsuyama J, Sato T, Nunez PP, Uematsu H, Hoshino E
    • Journal Title

      J Periodont Res 45

      Pages: 38-44

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Profiling of subgingival plaque biofilm microflora from periodontally healthy subjects and from subjects with periodontitis using quantitative real-time PCR.2010

    • Author(s)
      Abiko Y
    • Journal Title

      Journal of Periodontal Research 45(印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Host ss-globin-gene fragments of crevicular fluid as a biomarker in periodontal health and disease.2010

    • Author(s)
      Thaweboon B
    • Journal Title

      Journal of Periodontal Research 45

      Pages: 38-44

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Host β-globin-gene fragments of crevicular fluid as a biomarker in periodontal health and disease.2009

    • Author(s)
      Boonyanit Thaweboon
    • Journal Title

      J Periodontal Res 44(印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Profiling of subgingival plaque biofilm microflora from healthy and periodontitis subjects by quantitative real-time PCR.2009

    • Author(s)
      Yuki Abiko
    • Journal Title

      J Periodontal Res 44(印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Profiling of bacterial flora in crevices around titanium orthodontic anchor plates2007

    • Author(s)
      Sato R, Sato T, Takahashi I, Sugawara J, Takahashi N
    • Journal Title

      Clin Oral Impl Res 18

      Pages: 21-26

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nested PCR for the sensitive detection of cariogenic bacteria2007

    • Author(s)
      Sato T
    • Journal Title

      Cariology Today 3-4

      Pages: 17-20

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Profiling of bacterial flora in crevices around titanium orthodontic anchor plates2007

    • Author(s)
      Sato R
    • Journal Title

      Clin Oral Implants Res 18

      Pages: 21-26

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 歯内治療に伴う, 感染根管内細菌叢の変動に関する研究2009

    • Author(s)
      佐藤拓一, 八巻惠子, 橋本紀洋, 竹内裕尚, 安藤友希, 島内英俊, 高橋信博
    • Organizer
      第51回歯科基礎医学会学術大会
    • Place of Presentation
      新潟
    • Year and Date
      2009-09-11
    • Related Report
      2009 Final Research Report
  • [Presentation] 小児のう蝕歯面上のプラークバイオフィルム細菌叢構成細菌の定量的解析2009

    • Author(s)
      松山順子, 佐藤拓一, 他
    • Organizer
      第51回歯科基礎医学会学術大会
    • Place of Presentation
      新潟
    • Year and Date
      2009-09-11
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 歯内治療に伴う、感染根管内細菌叢の変動に関する研究2009

    • Author(s)
      佐藤拓一
    • Organizer
      第51回歯科基礎医学会学術大会
    • Place of Presentation
      新潟
    • Year and Date
      2009-09-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] 高齢者の歯肉縁下プラーク細菌叢への Porhyoromonas gingivalis の定着2009

    • Author(s)
      安彦友希, 佐藤拓一, 他
    • Place of Presentation
      新潟
    • Year and Date
      2009-09-10
    • Related Report
      2009 Final Research Report
  • [Presentation] 高齢者の歯肉縁下プラーク細菌叢へのPorphyromonas gingivalisの定着2009

    • Author(s)
      安彦友希
    • Organizer
      第51回歯科基礎医学会学術大会
    • Place of Presentation
      新潟
    • Year and Date
      2009-09-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] Metabolic activity of oral microbial ecosystem in health and disease2009

    • Author(s)
      Sato T
    • Organizer
      The1st Tohoku-Forsyth Symposium
    • Place of Presentation
      ボストン
    • Year and Date
      2009-03-10
    • Related Report
      2009 Final Research Report
  • [Presentation] Metabolic activity of oral microbial ecosystem in health and disease2009

    • Author(s)
      Takuichi Sato
    • Organizer
      The 1st Tohoku-Forsyth Symposium
    • Place of Presentation
      Boston, USA
    • Year and Date
      2009-03-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] Involvement of cough reflex impairment and silent aspiration of oral bacteria in postoperative pneumonia : A model of aspiration pneumonia2009

    • Author(s)
      Takuichi Sato
    • Organizer
      The 3rd International Symposium for Interface Oral Health Science
    • Place of Presentation
      仙台
    • Year and Date
      2009-01-16
    • Related Report
      2008 Annual Research Report
  • [Presentation] 感染根管治療に伴う根管内細菌叢の変動2009

    • Author(s)
      八巻惠子, 佐藤拓一, 島内英俊
    • Organizer
      第131回日本歯科保存学会2009年度秋季学術大会
    • Place of Presentation
      仙台
    • Related Report
      2009 Final Research Report
  • [Presentation] 感染根管治療に伴う根管内細菌叢の変動2009

    • Author(s)
      八巻惠子
    • Organizer
      第131回日本歯科保存学会秋季学術大会
    • Place of Presentation
      仙台
    • Related Report
      2009 Annual Research Report
  • [Presentation] 高齢者の歯肉縁下プラークバイオフィルムにおける歯周病関連菌の存在2007

    • Author(s)
      安彦友希
    • Organizer
      第49回歯科基礎医学会学術大会
    • Place of Presentation
      札幌市
    • Year and Date
      2007-08-30
    • Related Report
      2007 Annual Research Report
  • [Book] Interface Oral Health Science 20072007

    • Author(s)
      Ito Y
    • Publisher
      Springer, Tokyo
    • Related Report
      2007 Annual Research Report
  • [Book] Interface Oral Health Science 20072007

    • Author(s)
      Abiko Y
    • Publisher
      Springer, Tokyo
    • Related Report
      2007 Annual Research Report
  • [Book] Interface Oral Health Science 20072007

    • Author(s)
      Sato T
    • Publisher
      Springer, Tokyo
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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