A study of the influence of occlusal activity on the characteristics of tongue and masticatory muscles using non-occlusal model animal

• Project/Area Number: 19592266
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 補綴理工系歯学
• Research Institution: Tsurumi University
• Principal Investigator: ISHIKAWA Chieko Tsurumi University, 歯学部, 助教 (70176163)
• Co-Investigator(Kenkyū-buntansha): YAMANE Akira 鶴見大学, 歯学部, 教授 (20166763)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 小眼球症マウス / 咬合 / シナプス形成 / AChR転換 / AChR除去 / mitf / 咬筋 / 二次元電気泳動 / プロテオーム解析 / 萌出不全 / 筋形成 / C2C12 / 筋線維数 / 筋線維断面積 / MCK / siRNA / AChR / Real time PCR

Research Abstract

The present study attempted to elucidate the contribution of occlusal activity to synaptogenesis in masticatory muscles. In the masseter of the mi/mi, the nAChR elimination initiated, but did not progress normally after 3 weeks of age, when the occlusal activity emerged in the +/+ mouse, whereas the nAChR switch progressed normally during the entire period of synaptogenesis. The mRNA expression patterns of nAChR subunits in the temporalis and digastric of the mi/mi differed from those in its masseter. These findings suggest that, in the masseter, occlusal activity is essential for the completion of nAChR elimination, but not for the nAChR switch, and that the contribution of occlusal activity to synaptogenesis varies among the masticatory muscles.

Research Products

• [Journal Article] TGFβ3 is expressed in differentiating muscle of the embryonic mouse tongue (2010)
  • Author(s): YAMANE, A., FUKUI, T., SUGA, T., IIDA, R., MORITO, M
  • Journal Title: Int. J. Dev. Biol 54(1) Pages: 221-226
  • Peer Reviewed
• [Journal Article] Changes in the expression of myosins during postnatal development of masseter muscle in the microphthalmic mouse (2010)
  • Author(s): KATAYMA, R., YAMANE, A., FUKUI, T
  • Journal Title: Open Dent. J 4 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Changes in the expression of myosins during postnatal development of masseter muscle in the microphthalmic mouse (2010)
  • Author(s): KATAYMA, R., YAMANE, A., FUKUI, T.
  • Journal Title: Open Dent.J. 4 Pages: 1-7
  • Peer Reviewed
• [Journal Article] TGFβ3 is expressed in differentiating muscle of the embryonic mouse tongue (2010)
  • Author(s): YAMANE, A., FUKUI, T., SUGA, T., IIDA, R., MORITO, M
  • Journal Title: Int.J.Dev.Biol. 54 Pages: 221-226
  • Peer Reviewed
• [Journal Article] Effect of third molar absence on development of masticatory muscles in EL mice (2010)
  • Author(s): NARIYAMA, M., KOTA, Y., TASHIMA, I., TOMOHIRO, T., YAMANE, A., ASADA, Y.
  • Journal Title: Pediatric Dent.J. (In press)
  • NAID: 10027485798
  • Peer Reviewed
• [Journal Article] BMP-2 Regulates the Formation of Oral Sulcus in Mouse Tongue by Altering the Balance between TIMP-1 and MMP-13 (2010)
  • Author(s): FUKUI, T., SUGA, T., IIDA, R., MORITO, M., LUAN, X., DIEKWISCH, T.G.H., NAKAMURA, Y., YAMANE, A.
  • Journal Title: Anat.Rec. (In press)
  • Peer Reviewed
• [Journal Article] Contribution of occlusal activity to synaptogenesis in masticatory muscles (2009)
  • Author(s): KOTA, Y., YAMANE, A., TOMOHIRO, T., ASADA, Y
  • Journal Title: J. Dent. Res 88(8) Pages: 768-772
  • Peer Reviewed
• [Journal Article] Effects of clenbuterol, aβ2-adrenergic agonist, on sizes of masseter, temporalis, digastric, and tongue muscles (2009)
  • Author(s): ISHIKAWA, C., OGAWA, T., IKAWA, T., YAMANE, A
  • Journal Title: Open Dent. J 3 Pages: 191-196
  • Peer Reviewed
• [Journal Article] Availability of saliva for the assessment of alterations in the autonomic nervous system caused by physical exercise training (2009)
  • Author(s): YOSHINO, Y., YAMANE, A., SUZUKI, M., NAKAGAWA, Y
  • Journal Title: Arch. Oral Biol 54(11 Pages: 977-985
  • Peer Reviewed
• [Journal Article] Contribution of occlusal activity to synaptogenesis in masticatory muscles (2009)
  • Author(s): KOTA, Y., YAMANE, A., TOMOHIRO, T., ASADA, Y.
  • Journal Title: J.Dent.Res. 88 Pages: 768-772
  • Peer Reviewed
• [Journal Article] Effects of clenbuterol, a β2-adrenergic agonist, on sizes of masseter, temporalis, digastric, and tongue muscles (2009)
  • Author(s): ISHIKAWA, C., OGAWA, T., IKAWA, T., YAMANE, A.
  • Journal Title: Open Dent.J. 3 Pages: 191-196
  • Peer Reviewed
• [Journal Article] Availability of saliva for the assessment of alterations in the autonomic nervous system caused by physical exercise training (2009)
  • Author(s): YOSHINO, Y., YAMANE, A., SUZUKI, M., NAKAGAWA, Y.
  • Journal Title: Arch.Oral Biol.54 54 Pages: 977-985
  • Peer Reviewed
• [Journal Article] シリーズ 分析機器-何がわかるか 「リアルタイムPCR」 (2009)
  • Author(s): 山根明
  • Journal Title: DE 171 Pages: 30-32
  • Peer Reviewed
• [Journal Article] ビスホスホネートの薬理 (2008)
  • Author(s): 山根 明
  • Journal Title: 歯界展望 111 Pages: 1094-1097
• [Journal Article] Extracellular matrix-mediated tissue remodeling following axial movement of teeth (2007)
  • Author(s): LUAN, X., ITO, Y., HOLLIDAY, S., WALKER, C., DANIELL, J., GALANG, T.M., FUKUI, T. YAMANE, A., BEGOLE, E., EVANS, C., DIEKWISCH, T.G. H
  • Journal Title: J. Histochem. Cytochem 55(2) Pages: 127-140
  • Peer Reviewed
• [Journal Article] BMP2, BMP4, and their receptors are expressed in the differentiating muscle tissues of mouse embryonic tongue (2007)
  • Author(s): SUGA, T., FUKUI, T., SHINOHARA, A., LUAN, X., DIEKWISCH, T.G.H., MORITO, M., YAMANE, A
  • Journal Title: Cell Tissue Res 329(1) Pages: 103-117
  • Peer Reviewed
• [Journal Article] Characterization of excess hard tissue occurring in the mesio-labial surface of the mandibular first molar in microphthalmic mouse (2007)
  • Author(s): TOMOHIRO, T., YAMANE, A., ASADA, Y
  • Journal Title: Archs. Oral Biol 52(9) Pages: 828-835
  • Peer Reviewed
• [Journal Article] Expressions of BMP2, BMP4, and their receptors during the development of mouse tongue muscle (2007)
  • Author(s): SUGA, T., FUKUI, T., MORITO, M., YAMANE, A
  • Journal Title: Jpn. J. Gerodontol 22(3) Pages: 280-287
  • NAID: 10025742851
  • Peer Reviewed
• [Journal Article] Extracellular matrix-mediated tissue remodeling following axial movement of teeth (2007)
  • Author(s): LUAN, X., ITO, Y., HOLLIDAY, S., WALKER, C., DANIELL, J., GALANG, T. M., FUKUI, T. YAMANE, A., BEGOLE, E., EVANS, C., DIEKWISCH, T. G. H.
  • Journal Title: J. Histochem. Cytochem. 55(2):, 2007 55 Pages: 127-140
  • Peer Reviewed
• [Journal Article] BMP2, BMP4, and their receptors are expressed in the differentiating muscletissues of mouse embryonic tongue (2007)
  • Author(s): SUGA, T., FUKUI, T., SHINOHARA, A., LUAN, X., DIEKWISCH, T. G. H., MORITO, M., YAMANE, A.
  • Journal Title: Cell Tissue Res. 329 Pages: 103-117
  • Peer Reviewed
• [Journal Article] Characterization of excess hard tissue occurring in the mesio-labial surface of the mandibular first molar in microphthalmic mouse 07 (2007)
  • Author(s): TOMOHIRO, T., YAMANE, A., ASADA, Y.
  • Journal Title: Archs. Oral Biol. 52(9): 828-835, 20 52 Pages: 828-835
  • Peer Reviewed
• [Journal Article] Expressions of BMP2, BMP4, and their receptors during the development of mouse tongue muscle (2007)
  • Author(s): SUGA, T., FUKUI, T., MORITO, M., YAMANE, A.
  • Journal Title: Jpn. J. Gerodontol. 22 Pages: 280-287
  • NAID: 10025742851
  • Peer Reviewed
• [Journal Article] Characteristics of masticatory and tongue muscles (2007)
  • Author(s): YAMANE, A., FUKUI. T.
  • Journal Title: J. Oral Biosci. 49 Pages: 206-210
  • NAID: 10027102855
  • Peer Reviewed
• [Journal Article] 舌筋、咀嚼筋の発生、発達、再生 (2007)
  • Author(s): 山根 明、天野 修
  • Journal Title: 口腔組織培養学会誌 16 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Effect of third molar absence on development of masticatory muscles in EL mice
  • Author(s): NARIYAMA, M., KOTA, Y., TASHIMA, I., TOMOHIRO, T., YAMANE, A., ASADA, Y
  • Journal Title: Pediatric Dent. J (In press)
  • NAID: 10027485798
  • Peer Reviewed
• [Journal Article] BMP-2 Regulates the Formation of Oral Sulcus in Mouse Tongue by Altering the Balance between TIMP-1 and MMP-13
  • Author(s): FUKUI, T., SUGA, T., IIDA, R., MORITO, M., LUAN, X., DIEKWISCH, T.G.H., NAKAMURA, Y., YAMANE, A
  • Journal Title: Anat. Rec (In press)
  • Peer Reviewed
• [Journal Article] TGFβ3 is expressed in differentiating muscle of the embryonic mouse tongue
  • Author(s): YAMANE, A., FUKUI, T., SUGA, T., IIDA, R., M ORITO, M.
  • Journal Title: Int. J. Dev. Biol. (In press)
  • Peer Reviewed
• [Journal Article] Contribution of occlusal activity on synaptogenesis in mastica tory muscles
  • Author(s): KOUTA, Y., YAMANE, A., TOMOHIRO, T., ASAD A, Y.
  • Journal Title: J. Dent. Res. (In press)
  • Peer Reviewed
• [Presentation] マウス胎仔舌発生におけるBMP2の機能 (2010)
  • Author(s): 山根明、福井只美
  • Organizer: 第83回日本薬理学会学会年会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-03-16
• [Presentation] 歯が欠損している小眼球症マウスの咀嚼筋におけるミオシン重鎖の発現 (2009)
  • Author(s): 山根明
  • Organizer: 筋生理の集い
  • Place of Presentation: 東京慈恵会医科大
  • Year and Date: 2009-12-19
• [Presentation] トリグリセロール蓄積がラット骨格筋のエネルギー代謝関連遺伝子発現に及ぼす影響 (2009)
  • Author(s): 金子周平、飯田良平、菅武雄、福井只美、山根明、森戸光彦
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜パシフィコ
  • Year and Date: 2009-12-12
• [Presentation] 血小板由来増殖因子アンタゴニストが培養マウス舌筋細胞の増殖、分化に及ぼす影響 (2009)
  • Author(s): 鈴木栄里佳、福井只美、山根明、中村芳樹
  • Organizer: 第46回日本口腔組織培養学会学術大会
  • Place of Presentation: 昭和大学、東京
  • Year and Date: 2009-12-05
• [Presentation] 小眼球症マウスの生後発達過程における咬筋ミオシン重鎖mRNA発現の経時的変化 (2009)
  • Author(s): 片山理奈、福井只美、山根明
  • Organizer: 第51回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 朱鷺メッセ、新潟コンベンションセンター
  • Year and Date: 2009-09-10
• [Presentation] 小眼球症マウスの生後発達過程における咬筋ミオシン重鎖mRNA発現の経時的変化 (2009)
  • Author(s): 片山理奈、福井只美、山根明
  • Organizer: 第51回歯科基礎医学会学術大会ならびに総会、
  • Place of Presentation: 朱鷺メッセ、新潟コンベンションセンター
  • Year and Date: 2009-09-10
• [Presentation] 高脂肪食を摂取させたラット咬筋におけるアセチルコリン受容体サブユニットの発現 (2009)
  • Author(s): 山根明、金子周平、飯田良平、菅武雄、福井只美、篠原暁子、森戸光彦
  • Organizer: 第20回日本老年歯科医学会総会・学術大会
  • Place of Presentation: 横浜パシフィコ、横浜
  • Year and Date: 2009-06-19
• [Presentation] 高脂肪食を摂取させたラット骨格筋におけるトリグリセロールの蓄積について (2009)
  • Author(s): 金子周平、飯田良平、菅武雄、福井只美、篠原暁子、山根明、森戸光彦
  • Organizer: 第20回日本老年歯科医学会総会・学術大会
  • Place of Presentation: 横浜パシフィコ、横浜
  • Year and Date: 2009-06-19
• [Presentation] 高脂肪食を摂取させたラット咬筋におけるアセチルコリン受容体サブユニットの発現 (2009)
  • Author(s): 山根明、金子周平、飯田良平、菅武雄、福井只美、篠原暁子、森戸光彦
  • Organizer: 第20回日本老年歯科医学会総会・学術大会
  • Place of Presentation: 横浜パシフィコ、横浜、
  • Year and Date: 2009-06-19
• [Presentation] 高脂肪食を摂取させたラット骨格筋におけるトリグリセロールの蓄積について (2009)
  • Author(s): 金子周平、飯田良平、菅武雄、福井只美、篠原暁子、山根明、森戸光彦
  • Organizer: 第20回日本老年歯科医学会総会・学術大会
  • Place of Presentation: 横浜パシフィコ、横浜、
  • Year and Date: 2009-06-19
• [Presentation] Study on adipogenic change of lingual muscles in the obese rat (2009)
  • Author(s): SAITO, T., YAMANE, A., KANEKO, S., OGAWA, T
  • Organizer: 87th General session & exhibition of the IADR
  • Place of Presentation: Miami Beach Convention Center, USA
  • Year and Date: 2009-04-02
• [Presentation] 器官培養系を用いたマウス舌形態形成の研究 (2009)
  • Author(s): 山根明、福井只美
  • Organizer: 生体運動合同班会議
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2009-01-11
• [Presentation] Contribution of occlusal activity on expression of MyHC in masseter muscle50th Korean academy of pediatric dentistry (2009)
  • Author(s): KOTA, Y., YAMANE, A., ASADA, Y
  • Organizer: SETEC
  • Place of Presentation: Seoul, Korea
• [Presentation] Contribution of occlusal activity on expression of MyHC in masseter muscle (2009)
  • Author(s): KOTA, Y., YAMANE, A., ASADA, Y.
  • Organizer: 50th Korean academy of pediatric dentistry
  • Place of Presentation: SETEC, Seoul, Korea
• [Presentation] Klothoマウス骨格筋萎縮における蛋白質分解系について (2008)
  • Author(s): 山根明、飯田良平、菅武雄、金子周平、森戸光彦
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-12
• [Presentation] 分化過程のマウス舌筋細胞におけるTGFβおよびその受容体、シグナル伝達因子の発現 (2008)
  • Author(s): 山根明、福井只美
  • Organizer: 第50回歯科基礎医学会学術大会・総会
  • Place of Presentation: 有明コンベンションセンター
  • Year and Date: 2008-09-25
• [Presentation] Synaptogenesis of craniofacial muscles is affected by occlusal activity (2008)
  • Author(s): KOUTA, Y., TOMOHIRO, T., YAMANE, A., ASADA, Y
  • Organizer: 87th General Session & Exhibition of the IADR
  • Place of Presentation: Metro Toronto Convention Center
  • Year and Date: 2008-07-03
• [Presentation] Lunch & Learn Potential Function of small RNA in Craniofacial development (2008)
  • Author(s): YAMANE, A.
  • Organizer: 87th General Session & Exhibition of the IADR
  • Place of Presentation: Metro Toronto Convention Center
  • Year and Date: 2008-07-03
• [Presentation] The effect of oral appliance therapy on obstructive sleep apnea (2008)
  • Author(s): ISHIKAWA, C, OGAWA, T., SHIGETA, Y., et al.
  • Organizer: 87th General Session & Exhibition of the IADR
  • Place of Presentation: Metro Toronto Convention Center
  • Year and Date: 2008-07-02
• [Presentation] 老化促進(Klotho)マウス咬筋、舌筋、腓腹筋における蛋白質分解系について (2008)
  • Author(s): 飯田良平、菅武雄、金子夏樹、山根明、森戸光彦
  • Organizer: 第19回日本老年歯科医学会総会・学術大会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2008-06-12
• [Presentation] 歯の萌出不全モデルマウスを用いた咀嚼筋のプロテオーム解析 (2008)
  • Author(s): 古宇田ゆき、山根明、友廣忠文、田島格、小西博和、有田浩一朗、朝田芳信
  • Organizer: 第46回日本小児歯科学会大会
  • Place of Presentation: 大宮ソニックシティ
  • Year and Date: 2008-06-12
• [Presentation] Function of occlusal activity in the synaptogenesis and myogenesis of masseter muscle (2008)
  • Author(s): YAMANE, A., KOUTA, Y., SUGA, T., KANEKO, N., MORITO, N., ASADA, Y
  • Organizer: Gordon Research Conference "Craniofacial morphogenesis and tissue regeneration"
  • Place of Presentation: Il Ciocco, Italy
  • Year and Date: 2008-02-12
• [Presentation] Function of occlusal activity in the synaptogenesis and myogenesis of masseter muscle (2008)
  • Author(s): YAMANE, A., KOUTA, Y., SUGA, T., KANEKO, N., MORITO, N. and ASADA, Y.
  • Organizer: Gordon Research Conference "Craniofacial morphogenesis and tissue regeneration"
  • Place of Presentation: II Ciocco, Italy
  • Year and Date: 2008-02-12
• [Presentation] BMP2はTIMPとMMPの発現を調節することによりマウス舌側溝形成を制御している (2008)
  • Author(s): 福井只美、山根明
  • Organizer: 第67回日本矯正歯科学会大会
  • Place of Presentation: 幕張メッセ
• [Presentation] Effects of occlusal activity on masseter myogenesis and synaptogenesis (2007)
  • Author(s): KOUTA, Y., YAMANE, A., TASHIMA, I., TOMOHIRO, T., ARITA, K., ASADA, Y
  • Organizer: 55th Annual meeting of JADR
  • Place of Presentation: 鶴見大学
  • Year and Date: 2007-11-08
• [Presentation] 飼料性状の違いがマウス咬筋のシナプス形成に及ぼす影響 (2007)
  • Author(s): 古宇田ゆき、山根明、朝田芳信
  • Organizer: 第49回歯科基礎医学会学術大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2007-08-31
• [Presentation] The establishment of the snoring and OSA clinic in Tsurumi UNIV. School of Dental Medicine (2007)
  • Author(s): ISHIKAWA, C.,
  • Organizer: Academic Exchange Seminar
  • Place of Presentation: Jilin University
  • Year and Date: 2007-08-10
• [Presentation] 咬合様式の違いが咀嚼筋の筋タンパク発現に及ぼす影響 (2007)
  • Author(s): 古宇田ゆき、山根明、友廣忠文、有田浩一朗、田島格、朝田芳信
  • Organizer: 第45回日本小児歯科学会大会
  • Place of Presentation: タワーホール船堀
  • Year and Date: 2007-07-20
• [Book] スタディ歯科薬理学 (2009)
  • Author(s): 山根明
  • Total Pages: 260
  • Publisher: 永末書店
• [Book] スタディ歯科薬理学 (2009)
  • Author(s): 山根 明
  • Total Pages: 260
  • Publisher: 永末書店