The effect of cancer stem cell on Epithelial to Mesenchymal Transition.

• Project/Area Number: 19592295
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Okayama University
• Principal Investigator: YANAGI Yoshinobu Okayama University, 医学部・歯学部付属病院, 助教 (50284071)
• Co-Investigator(Kenkyū-buntansha): TSUJIGIWA Hidetugu 岡山大学, 大学院・医歯薬学総合研究科, 助教 (70335628) ; MURAKAMI Jyun 岡山大学, 大学院・医歯薬学総合研究科, 助教 (40362983)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 実験腫瘍学 / 臨床 / 薬理学 / 遺伝子 / 発現制御

Research Abstract

平成19年度 : 各種ヒト由来癌樹立細胞株を用いて、癌幹細胞同定に先立って、増殖能、転移能、および癌幹細胞マーカー候補のCD133発現を検討した。さらに「上皮間充織転換」関連因子、上皮マーカーとしてRhoファミリー(Cdc42、Rac1)、間充織マーカーとしてVimentin、Fibronectinの発現状況を検討し、それらによる転移ならびに血管新生への影響を検討した。 平成20年度 : 「癌幹細胞」「上皮間充織転換」規定因子発現に基づく抗腫瘍効果予測法の検討。 ヒト癌幹細胞(CELPROGEN社 ; 2008年発売)購入を予定していたが、製品に不備があり、購入を見合わせた。平成20年は癌細胞株にカドヘリン遺伝子siRNAを導入し、上皮-間充織転換を強制的に起こさせる試みを行った。

Research Products

• [Journal Article] Secreted frizzled related protein (sFRP)-2 inhibits bone formation and promotes cell proliferation in ameloblastoma (2009)
  • Author(s): Tamamura R, Tsujigiwa H, Borkosky SS, Gunduz M, Nagatsuka H
  • Journal Title: Oral Oncol (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Dynamic contrast-enhanced magnetic resonance imaging for estimating tumor proliferation and microvessel density of oral squamous cell carcinomas (2008)
  • Author(s): Unetsubo T, Konouchi H, Yanagi Y, Murakami J, Fujii M, Matsuzaki H, Hisatomi M, Nagatsuka H, Asaumi JI
  • Journal Title: Oral Oncol Nov 20.(Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Heparanase and vascular endothelial growth factor expression in the progression of oral mucosal melanoma (2008)
  • Author(s): Rivera RS, Nagatsuka H, Siar CH, Gunduz M, Tsujigiwa H, Han PP, Katase N, Tamamura R, Ng KH, Naomoto Y, Nakajima M, Nagai N
  • Journal Title: Oncol Rep 19(3) Pages: 657-61
  • Peer Reviewed
• [Journal Article] Heparanase and vascular endothelial growth factor expression in the progression of oral mucosal melanoma. (2008)
  • Author(s): Rivera RS
  • Journal Title: Oncol Report 19(3) Pages: 657-661
  • Peer Reviewed
• [Journal Article] Microsatellite instability and clinicopathological features in esophageal squamous cell cancer (2007)
  • Author(s): Matsumoto Y, Nagasaka T, Kambara T, Hoshizima N, Murakami J, Sasamoto H, Hosokawa M, Naomoto Y, Isozaki H, Shimizu K. Tanaka N, Matsubara N
  • Journal Title: Oncol Rep 18(5) Pages: 1123-7
  • Peer Reviewed
• [Journal Article] Depletion of O6-methylguanine-DNA methyltransferase by O6-benzylguanine enhances 5-FU cytotoxicity in colon and oral cancer cell lines (2007)
  • Author(s): Murakami J, Lee YJ, Kokeguchi S, Tsujigiwa H, Asaumi J, Nagatsuka H, Fukui K, Kuroda M, Tanaka N, Matsubara N
  • Journal Title: Oncol Rep 17(6) Pages: 1461-7
  • Peer Reviewed
• [Journal Article] Depletion of 06-methylguanine-DNA methyltransferase by 06-benzylguanine enhances 5-FU cytotoxicity in colon and oral cancer cell lines. (2007)
  • Author(s): Murakami J, et. al.
  • Journal Title: Oncol Rep. 17 Pages: 1461-1467
  • Peer Reviewed
• [Journal Article] Secreted frizzled related protein (sFRP)-2 inhibits bone formation and promotes cell proliferation in ameloblastoma
  • Author(s): Sathi GA
  • Journal Title: Oral Oncology (in press)
  • Peer Reviewed
• [Journal Article] Dynamic contrast-enhanced magnetic resonance imaging for estimating tumorproliferation and microvessel density of oral squamous eel 1 carcinomas.
  • Author(s): Unetsubo T
  • Journal Title: Oral Oncology (in press)
  • Peer Reviewed
• [Presentation] 大腸鋸歯状polyp及び腺管腺腫におけるメチル化、K-ras, B-raf遺伝子、MSIの解析と比較 (2008)
  • Author(s): 長尾厚樹、松原長秀、神原健、能登原憲司、笹本博美、村上純、内田卓之、星島直子、田中紀章
  • Organizer: 第63回日本消化器外科学会総会
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2008-07-17
• [Presentation] Dynamic contrast-enhanced magnetic resonance imaging for estimatimg tumor proliferation and microvessel density of oral squamous cell carcinoma (2008)
  • Author(s): Unetsubo T, Konouchi H, Yanagi Y, Murakami J, Matsuzaki H, Asaumi JI
  • Organizer: The 7th Asian Congress of Oral and Maxillo-Facial Radiology, The 13th Conference of Clinical Diagnostics of JSOMR
  • Place of Presentation: Nara, Japan
• [Presentation] A novel benzylidene lactam compound, KNK437 inhibits the invasion and angiogenesis in oral cancer cell lines (2008)
  • Author(s): J Murakami, J Asaumi
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] 口腔癌細胞株に対する新規抗癌剤KNK437による血管新生阻害と癌浸潤阻害作用について (2008)
  • Author(s): 村上純、柳文修、此内浩信、浅海淳一
  • Organizer: NPO法人日本歯科放射線学会第5回総会・第49回学術大会
  • Place of Presentation: 愛知
• [Presentation] A novel benzylidene lactam compound. KNK437 inhibits the invasion and angiogenesis in oral cancer cell lines (2008)
  • Author(s): 村上 純
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
• [Book] Inc Bacterial DNA, DNA polymerase and DNA helicases (2009)
  • Author(s): Jun Murakami, Jun-ichi Asaumi, Hidetsugu Tsujigiwa, Masao Yamada, Susumu Kokeguchi, itoshi Nagatsuka, Tatsuo Yamamoto
  • Publisher: You-Jin Nova Science Publishers(in press)
• [Book] Nova Science Publishers (2008)
  • Author(s): Jun Murakami, Jun-ichi Asaumi, Hidetsugu Tsujigiwa, Hitoshi Nagatsuka, Masahiro Kuroda, You-Jin Lee and Susumu Kokeguchi
  • Publisher: Inc Progress in Oral Cancer Research
• [Book] Progress in Oral Cancer Research Recent Progress in Molecular Targets and Oncology Therapeutics (2008)
  • Author(s): Jun Murakami
  • Total Pages: 44
  • Publisher: Nova Science Publishers, Inc
• [Book] Progress in Oral Cancer Research (2008)
  • Author(s): Jun Murakami, et. al.
  • Total Pages: 197
  • Publisher: Nova Science Publishers, Inec. NY