An in vitro model of cartilage degradation by chondrocytes in a three-dimensional culture system

• Project/Area Number: 19592328
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Aichi Gakuin University
• Principal Investigator: KURITA Kenichi Aichi Gakuin University, 歯学部, 教授 (40133483)
• Co-Investigator(Kenkyū-buntansha): YAMASHITA Kyoko 愛知学院大学, 歯学部, 講師 (40231659)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 軟骨 / TIMP / CAM / Alginate beads / ECM / IL-1β / TGF-β / OA / HA

Research Abstract

The results of this study suggest that TIMP-1 plays a primary role in the prevention of articular cartilage destruction in its early stage but that TIMP-3 gradually takes over this role. Also, TGF-β was shown to regulate these TIMPs and act as a suppressor of articular cartilage destruction. These results suggest that TIMP-1 and TIMP-3 are closely involved in preventing the progression of joint disorders such as OA.