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Establishment of immortalized normal salivary glands epithelial cells and regeneration of salivary gland for post-irradiation

Research Project

Project/Area Number 19592331
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionHyogo College of Medicine

Principal Investigator

NOGUCHI Kazuma  Hyogo College of Medicine, 医学部, 講師 (50309473)

Co-Investigator(Kenkyū-buntansha) URADE Masahiro  兵庫医科大学, 医学部, 教授 (70104883)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords唾液腺 / 放射線 / 口腔乾燥 / 再生医療
Research Abstract

In individualized countries the majority of patients with head and neck cancer are treated with radiation therapy. Exposure of the major salivary glands to the ionizing radiation induced fibrosis fatty degeneration of the gland tissue and atrophy of the saliva-producting acinar cells. Saliva production is decreased in correlation with the irradiation dose and the gland volume exposed to the radiation field. Current management of radiation induced xerostomia includes the administration of saliva substitutes and sialogogues. Based on the principles of tissue engineering, we investigated the feasibility of engineering human salivary gland tissue for the treatment of salivary gland hypofunction. There are two major mechanisms that cause the limited life span of primary culture cells. One is the telomere-based senescene, and the other is telomere-independent senescene, which is thought to be controlled by the Rb/p16 and p53 pathways. To elucidate the regeneration of normal salivary gland cell, we established immortalized human salivary gland epithelial cell (hSalEC)s transduced with non-viral human gene (mutant Cdk4, cyclin D1, and hTERT). By Cdk4 and cyclinD1 transduction in combination with hTERT, we here established novel hSalEC cell line. This is a first report of successful establishment of hSalEC cells immortalization.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (3 results)

All 2008

All Journal Article (2 results) Presentation (1 results)

  • [Journal Article] ヒト正常唾液腺不死化細胞の樹立2008

    • Author(s)
      野口一馬、清野透、広本孝史、頭司雄介、田中徳昭、浦出雅裕
    • Journal Title

      日本歯科評論 68(9)

      Pages: 39-40

    • Related Report
      2009 Final Research Report
  • [Journal Article] ヒト正常唾液腺不死化細胞の樹立2008

    • Author(s)
      野口一馬, 他
    • Journal Title

      日本歯科評論 68(9)

      Pages: 39-40

    • Related Report
      2008 Annual Research Report
  • [Presentation] CDK4, Cyclin D1およびhTERT遺伝子導入によるヒト正常唾液腺培養細胞不死化の試み2008

    • Author(s)
      廣本孝史、野口一馬、頭司雄介、田中徳昭、浦出雅裕
    • Organizer
      日本口腔科学会総会
    • Place of Presentation
      福岡
    • Related Report
      2009 Final Research Report 2008 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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