Development of novel diagnostic system of oral cancer by HOX gene expression profiles

• Project/Area Number: 19592332
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Hokkaido University
• Principal Investigator: KASHIWAZAKI Haruhiko Hokkaido University, 北海道大学病院, 助教 (10344516)
• Co-Investigator(Kenkyū-buntansha): HAMADA Junichi 北海道大学, 遺伝子病制御研究所, 准教授 (50192703)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 臨床腫瘍学 / 遺伝子診断 / 遺伝子 / 癌 / 歯学

Research Abstract

リンパ節転移のあるヒト口腔扁平上皮癌組織で発現の高いHOXC5, HOXC6およびHOXC8が, リンパ節転移に関与しているのかどうかを調べるために, 各HOX遺伝子の発現プラスミドベクターを構築した. 各々のHOX遺伝子発現ベクターをヒト口腔扁平上皮癌細胞株にリポフェクション法で導入し, 各HOX mRNAおよび蛋白の発現をRT-PCR法およびウエスタンブロット法で確認した. リンパ節転移に重要と考えられる癌細胞とリンパ管内皮細胞との相互作用を解析するために, ヒト皮膚から樹立されたリンパ管内皮細胞の同定を行った. 現在, HOX遺伝子を強制発現させた癌細胞のリンパ管内皮細胞への接着性ならびにリンパ管内皮細胞の培養上清に対する運動性などを検討している.

Research Products

• [Journal Article] Localization and characterization of g-glutamyl cyclotransferase in cancer cells (2009)
  • Author(s): Azumi, K., Ikeda, Y., Takeuchi, T., Nomura, T., Sabau, S.V., Hamada, J., Okada, F., Hosokawa, M. and Yokosawa, H
  • Journal Title: Molecular Medicine Reports 2 Pages: 385-391
  • Peer Reviewed
• [Journal Article] PAX4 has the potential to function as a tumor suppressor in human melanoma. (2008)
  • Author(s): Hata, S., Hamada, J., Maeda, K., Murai, T., Tada, M., Furukawa, H., Tsutsumida, A., Saito, A., Yamamoto Y. and Moriuchi, T
  • Journal Title: Int. J. Oncol 33 Pages: 1065-1071
  • Peer Reviewed
• [Journal Article] Anti-tumor activity of ESX1 on cancer cells harboring oncogenic K-ras mutation. Biochem. Biophys (2008)
  • Author(s): Nakajima, J., Ishikawa S., Hamada, JI., Yanagihara, M., Koike, T and Hatakeyama, M.
  • Journal Title: Res. Commun 370 Pages: 189-194
  • Peer Reviewed
• [Journal Article] Gene Expression Profile Changes Correlated with Lymph Node Metastasis in Oral Squamous Cell Carcinoma (2008)
  • Author(s): Kashiwazaki H, Hassan NMM, Hamada J, Moriuchi T, Yamazaki Y, Tei K, Totsuka Y, Inoue N.
  • Journal Title: Odontology 96 Pages: 38-43
  • NAID: 10024565109
  • Peer Reviewed
• [Journal Article] Presence of dominant negative mutation of TP53 is a risk of early recurrence in oral cancer (2008)
  • Author(s): Hassan NMM, Tada M, Hamada J, Kashiwazaki H, Kameyama T, Akhter R, Yamazaki Y, Yano M, Inoue N, Moriuchi T
  • Journal Title: Cancer Lett 270(1) Pages: 108-19
  • Peer Reviewed
• [Journal Article] Relationship between periodontal status and physical fitness in an elderly population of nonsmokers in Bangladesh (2008)
  • Author(s): Akhter R, Hassan NMM, Moriya S, Kashiwazaki H, Inoue N, Morita M
  • Journal Title: J Am Geriatr Soc 56(12) Pages: 2368-70
  • Peer Reviewed
• [Journal Article] Gehe Expression Profile Changes Correlated with Lymph Node Metastasis in Oral Squamous Cell Carcinoma (2008)
  • Author(s): Kashiwazaki H, et.al.
  • Journal Title: Odontology 96 Pages: 38-43
  • Peer Reviewed
• [Journal Article] Anti-tumor activity of ESX1 on cancer cells harboring oncogeni c K-ras mutation (2008)
  • Author(s): Nakajima, J., et.al.
  • Journal Title: Biochem. Biophys. Res. Commun 370 Pages: 189-194
  • Peer Reviewed
• [Journal Article] Gene Expression Profile Changes Correlated with Lymph Node Metastasis in Oral Squamous Cell Carcinoma. (2008)
  • Author(s): Kashiwazaki H, et. al.
  • Journal Title: Odontology(確定) 86(未定)
  • NAID: 10024565109
  • Peer Reviewed
• [Journal Article] Laminin-421 produced by lymphatic endothelial cells induces chemotaxis for human melanoma cells
  • Author(s): Saito, N., Hamada, J., Furukawa, H., Tsutsumida, A., Oyama, A., Funayama, E., Saito, A., Tsuji, T., Tada, M., Moriuchi, T. and Yamamoto, Y
  • Journal Title: Pig. Cell Melanoma Res. (in press)
  • Peer Reviewed
• [Presentation] Presence of dominant-negative p53 mutation is a risk of early recurrence in oral squamous cell carcinoma (2007)
  • Author(s): Haruhiko Kashiwazaki, Nur Mohammad Monsur Hassan, Yutaka Yamazaki, Kanchu Tei, Yoshimasa Kitagawa, Yasunori Totsuka, Nobuo Inoue
  • Organizer: AAOMS/JSOMS/KAOMS 89th Annual meeting
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2007-10-12
• [Presentation] p53遺伝子の優性阻害性変異は口腔扁平上皮癌の早期再発を予測しうる (2007)
  • Author(s): 柏崎晴彦, ハッサン・ヌル・モハマド・モンスル, 山崎 裕, 鄭 漢忠, 北川善政, 戸塚靖則, 井上農夫男
  • Organizer: 第52回日本口腔外科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-09-29
• [Presentation] Relationship between betel-quid chewing and established periodontitis. (2006)
  • Author(s): Rahena Akhter, Nur Mohammad Monsur Hassan, Jun Aida, Haruhiko Kashiwazaki, Manabu Morita
  • Organizer: 第18回日本老年歯科医学会
  • Place of Presentation: 札幌