Analysis of pathogenic mechanism of chronic pain in oro-facial aria using a index of CRF gene expression in hypothalamus
| Project/Area Number |
19592401
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Social dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
SHOJI Noriaki Tohoku University, 病院, 講師 (70250800)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIMOTO Koreaki 東北福祉大学, 総合福祉学部, 講師 (30361158)
SASANO Takashi 東北大学, 大学院・歯学研究者, 教授 (10125560)
SATOH Shizuko 東北大学, 大学院・歯学研究者, 助教 (60225274)
SUGAWARA Yumiko 東北大学, 病院, 助教 (30235866)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 痛みストレス / 視床下部-下垂体-副腎系 / CRF遺伝子 / hnRNA / 視床下部-下垂体-副腎系(HPA axis) / 急性痛 / CRHmRNA / CRH hnRNA / Real-time PCR解析 / 視床下部-下垂体-副腎系(HPA. axis) / 慢性痛 / ACTH / corticosterone / カプサイシン |
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| Research Abstract |
Pain is one of the physical stressor for humans. In this study, we performed quantitative analysis of CRF gene transcription in hypothalamic PVN under the condition of tongue pain stress in rats. Plasma adrenocorticotropic hormone (ACTH) secretion was peak at 30 min after the capsaicin injection to tongue prior to gradually increasing of plasma corticosterone, indicating that tongue pain is stressor enough to up-regulate hypothalamo-pituitary- adrenocortical axis. CRF hnRNA 15 min after the injection was up-regulated 3 fold than that of control rats. This report is the first study which succeeded to detect CRF hnRNA by quantitative real-time PCR analysis.
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Report
(4 results)
Research Products
(28 results)
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[Presentation] 小唾液腺分泌量の測定方法について2009
Author(s)
庄司憲明, 佐藤しづ子, 飯久保正弘, 古内寿, 阪本真弥, 笹野高嗣
Organizer
第4回Oral Medicine and IVR研究会
Place of Presentation
札幌
Year and Date
2009-10-23
Related Report
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