• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Hydrogen Sulfide Production from Cysteine and Homocysteine by Periodontal and Oral Bacteria

Research Project

Project/Area Number 19592409
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Social dentistry
Research InstitutionKyushu Dental College

Principal Investigator

YOSHIDA Yoshida  Kyushu Dental College, 歯学部, 助教 (20364151)

Co-Investigator(Kenkyū-buntansha) TAKEHARA Tadamichi  九州歯科大学, 歯学部, 教授 (00038879)
ANSAI Toshihiro  九州歯科大学, 歯学部, 准教授 (80244789)
AWANO Shuji  九州歯科大学, 歯学部, 講師 (20301442)
SOH Inho  九州歯科大学, 歯学部, 助教 (10285463)
HAMASAKI Tomoko  九州歯科大学, 歯学部, 助教 (60316156)
Co-Investigator(Renkei-kenkyūsha) NAKAYAMA Koji  長崎大学, 歯学部, 教授 (80150473)
OHARA Naoya  国立感染症研究所, 室長 (70223930)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords歯周病 / 酵素活性 / 迅速検出 / 口腔細菌 / システイン / ホモシステイン / 硫化水素 / 診断
Research Abstract

各種口腔細菌の培養液を用いて、ホモシステインおよびシステインを基質とした、ホモシステイン分解活性およびシステイン分解活性を各々の基質について測定した。菌数が既知の菌液のホモシステイン分解活性あるいはシステイン分解活性を測定することにより、単位菌数あたりの酵素活性を測定した。また、ホモシステインおよびシステインを基質とした場合のそれぞれの分解活性の高い口腔細菌種を同定した。また、ホモシステインおよびシステインの分解産物である硫化水素を測定することにより口腔細菌の迅速検出系を構築し、その評価を行った。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (12 results)

All 2009 2008 2007 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (3 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Identification of the genes associated with a virulent strain of Porphyromonas gingivalis using the subtractive hybridization technique.2008

    • Author(s)
      Tachibana-Ono, M., Yoshida, A., et al
    • Journal Title

      Oral Microbiol. Immunol. 23

      Pages: 84-87

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The prevalence of periodontopathogenic bacteria in saliva is linked to Deriodontal health status and oral malodour2008

    • Author(s)
      Kurata, H., Awano, S., Yoshida, A, et al
    • Journal Title

      J. Med. Microbiology 57

      Pages: 636-642

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Loop-mediated isothermal amplification method for the rapid detection of Enterococcus faecalis in infected root canals.2007

    • Author(s)
      Kato, H., Yoshida, A., Ansai, T., Watari, H., Notomi, T. and Takehara, T
    • Journal Title

      Oral Microbiol.Immunol. 22

      Pages: 214-219

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid detection of Actinobacillus actinomycetemcomitans using Loop-Mediated Isothermlal Amplification Method.2007

    • Author(s)
      Osawa, R., Yoshida, A., et. al.
    • Journal Title

      Oral Microbiol.Immunol. 22

      Pages: 252-259

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid Detection of the Cariogenic Pathogens Streptococcus mutans and Streptococcus sobrinus using Loop-Mediated Isothermal Amplification2007

    • Author(s)
      Nagashima, S., Yoshida, A., et. al.
    • Journal Title

      Oral Microbiol.Immunol. 22

      Pages: 361-368

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hydrogen sulfide production from cysteine and homocysteine by periodontal and oral bacteria

    • Author(s)
      Akihiro Yoshida, Mamiko Yoshimura, Naoya Ohara, Shigeru Yoshimura, Shiori Nagashima, Tadamichi Takehara, Koji Nakayama
    • Journal Title

      Journal of Periodontology 印刷中

      Pages: 0-0

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Presentation] 生菌と死菌/損傷菌を区別した定量的口腔細菌検出法の開発2009

    • Author(s)
      吉田明弘
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2008 Final Research Report
  • [Presentation] Transcriptional Profiling of Streptococcus mutans Biofilms Co-cultured with St-reotococcus gordonii2008

    • Author(s)
      Yoshida, A., et al
    • Organizer
      86th General Session & Exhibition of the IADR
    • Place of Presentation
      Metro Toronto Convention Centre, on, Canada
    • Related Report
      2008 Annual Research Report
  • [Presentation] Prevotella intermediaおよびPrevotella nigrescensに特異的な遺伝子群の解析.2007

    • Author(s)
      正清 義朗, 吉田 明弘, 安細 敏弘, 新谷 泰之, 高橋 優介, 竹原 直道
    • Organizer
      口腔衛生学会
    • Place of Presentation
      東京
    • Related Report
      2007 Annual Research Report
  • [Book] バイオフィルムの基礎と制御2008

    • Author(s)
      中山二郎, 吉田明弘
    • Publisher
      株式会社エヌ・ティー・エス
    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.kenkoyobou.net/college/index.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.kenkoyobou.net/college/index.html

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi