Experimental pressure ulcers : analysis of the pathogenesis and development of the preventive and therapeutic procedures using animal models

• Project/Area Number: 19592492
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical nursing
• Research Institution: Hiroshima University
• Principal Investigator: KAWAMATA Seiichi Hiroshima University, 大学院・保健学研究科, 教授 (30127641)
• Co-Investigator(Kenkyū-buntansha): KUROSE Tomoyuki 広島大学, 大学院・保健学研究科, 助教 (20363054)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 褥瘡 / 磁石 / 圧迫 / 虚血 / ラット / 麻酔

Research Abstract

研究成果の概要 : 褥瘡の実験モデルとして、ラットの腹壁を、1) 腹膜腔内に挿入した鉄板と体表に置いた重り、または2) 腹膜腔内と体表の磁石で圧迫した。50、100mmHgで2、3、4時間圧迫し、12時間、1、3、7日後に試料を採取して、顕微鏡標本で傷害を評価した。圧迫時間が長いほど傷害が重くなった。また、マイクロアレイで約3万種類の遺伝子から発現が大きく変動するものを絞り込み、炎症に関係深いものをリアルタイムPCRで解析した。

Research Products

• [Journal Article] Regeneration of peripheral nerve after transplantation of CD133(+) cells derived from human peripheral blood (2009)
  • Author(s): Kijima Y, Ishikawa M, Sunagawa T, Nakanishi K, Kamei N, Yamada K, Tanaka N, Kawamata S, Asahara T, Ochi M
  • Journal Title: J Neurosurg 110(4) Pages: 758-767
  • Peer Reviewed
• [Journal Article] The skeletal muscle vascular supply closely correlates with the muscle fiber surface area in the rat (2008)
  • Author(s): Ichinose E, Kurose T, Daitoku D, Kawamata S
  • Journal Title: Arch Histol Cytol 71(1) Pages: 45-57
  • NAID: 10021998666
  • Peer Reviewed
• [Journal Article] Alteration in articular cartilage of rat knee joints after spinal cord injury (2008)
  • Author(s): Moriyama H, Yoshimura O, Kawamata S, Takayanagi K, Kurose T, Kubota A, Hosoda M, Tobimatsu Y
  • Journal Title: Osteoarthritis Cartilage 16(3) Pages: 392-398
  • Peer Reviewed
• [Journal Article] Regeneration of peripheral nerve after transplantation of CD133(+)cells derived from human peripheral blood (2008)
  • Author(s): Kijima Y
  • Journal Title: J Neurosurg (オンライン出版)
  • Peer Reviewed
• [Journal Article] The skeletal muscle vascular supply closely correlates with the muscle fiber surface area in the rat (2008)
  • Author(s): Ichinose E
  • Journal Title: Arch Histol Cytol 71 Pages: 45-57
  • Peer Reviewed
• [Journal Article] Alteration in articular cartilage of rat knee joints after spinal cord injury (2008)
  • Author(s): Moriyama H
  • Journal Title: Osteoarthritis Cartilage 16 Pages: 392-398
  • Peer Reviewed
• [Journal Article] Alteration in articular cartilage of rat knee joints after spinal cord injury. (2008)
  • Author(s): Moriyama H
  • Journal Title: Osteoarthritis Cartilage 16 Pages: 392-398
  • Peer Reviewed
• [Journal Article] Magnetically labeled neural progenitor cells, which are localized by magnetic force, promote axon growth in organotypic cocultures (2007)
  • Author(s): Hamasaki T, Tanaka N, Kamei N, Ishida O, Yanada S, Nakanishi K, Nishida K, Oishi Y, Kawamata S, Sakai N, Ochi M
  • Journal Title: Spine 32(21) Pages: 2300-2305
  • Peer Reviewed
• [Journal Article] Alteration of knee joint connective tissues during contracture formation in spastic rats after an experimentally induced spinal cord injury (2007)
  • Author(s): 6. Moriyama H, Yoshimura O, Kawamata S, Takemoto H, Saka Y, Tobimatsu Y
  • Journal Title: Connect Tissue Res 48(4) Pages: 180-187
  • NAID: 10021232123
  • Peer Reviewed
• [Journal Article] Role of osteoprotegerin in arterial calcification : development of new animal model (2007)
  • Author(s): Orita Y, Yamamoto H, Kohno N, Sugihara M, Honda H, Kawamata S, Mito S, Soe NN, Yoshizumi M
  • Journal Title: Arterioscler Thromb Vasc Biol 27(9) Pages: 2058-2064
  • Peer Reviewed
• [Journal Article] The time course and extent of motor neuron loss following spinal cord compression in rats (2007)
  • Author(s): Takemoto H, Yoshimura O, Moriyama H, Imagita H, Shirahama K, Kawamata S, Kajihara H, Tobimatus Y
  • Journal Title: 広島大学保健学ジャーナル 6(2) Pages: 111-117
  • NAID: 120001823940
  • Peer Reviewed
• [Journal Article] Changes in the rat subcutaneous-connective tissue after saline and histamine injection in relation to fluid storage and excretion. (2007)
  • Author(s): Daitoku D
  • Journal Title: Arch Histol Cytol 70 Pages: 29-41
  • Peer Reviewed
• [Journal Article] Role of osteoprotegerin in arterial calcification: development of new animal model. (2007)
  • Author(s): Orita Y
  • Journal Title: Arterioscler Thromb Vasc Biol 27 Pages: 2058-2064
  • Peer Reviewed
• [Journal Article] Magnetically labeled neural progenitor cells, which are localized by magnetic force, promote axon growth in organotypic cocultures. (2007)
  • Author(s): Hamasaki T
  • Journal Title: Spine 32 Pages: 2300-2305
  • Peer Reviewed
• [Journal Article] Alteration of knee joint connective tissues during contracture formation in spastic rats after an experimentally induced spinal cord injury. (2007)
  • Author(s): Moriyama H
  • Journal Title: Connect Tissue Res 48 Pages: 180-187
  • Peer Reviewed
• [Journal Article] The time course and extent of motor neuron loss following spinal cord compression in rats. (2007)
  • Author(s): Takemoto H
  • Journal Title: 広島大学保健学ジャーナル 6 Pages: 111-117
  • Peer Reviewed
• [Journal Article] Comparison between a weight compression and a magnet compression for experimental pressure ulcers in the rat. Histological studies and effects of anesthesia
  • Author(s): Hashimoto M, Kurose T, Kawamata S
  • Journal Title: Arch Histol Cytol (in press)
  • NAID: 10025096292
  • Peer Reviewed
• [Journal Article] Morphological changes in hindlimb muscles elicited by adjuvant-induced arthritis of the rat knee
  • Author(s): Ozawa J
  • Journal Title: Scand J Med Sci Sports (印刷中)
  • Peer Reviewed
• [Presentation] 体解剖見学で各種器官の大きさ概念がどう変わるか (2009)
  • Author(s): 川真田聖一、黒瀬智之
  • Organizer: 第114回日本解剖学会総会・学術集会
  • Place of Presentation: 岡山市
  • Year and Date: 2009-03-29
• [Presentation] 人体解剖見学で各種器官の大きさ概念がどう変わるか (2009)
  • Author(s): 川真田聖一
  • Organizer: 第114回日本解剖学会
  • Place of Presentation: 岡山市
• [Presentation] ホルムアルデヒド発生源であるご遺体への対策 (2008)
  • Author(s): 川真田聖一
  • Organizer: 日本解剖学会第63回中国・四国支部学術集会
  • Place of Presentation: 出雲市
  • Year and Date: 2008-10-25
• [Presentation] ラット褥瘡実験モデルの作製と褥瘡関連遺伝子の解析 (2008)
  • Author(s): 黒瀬智之、橋本将和、川真田聖一
  • Organizer: 第10回日本褥瘡学会学術集会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-08-29
• [Presentation] ラット脱神経筋におけるナトリウムチャネル遺伝子発現の変化 (2008)
  • Author(s): 小澤淳也、川真田聖一、黒瀬智之、橋本将和、山岡薫
  • Organizer: 第43回日本理学療法学術集会
  • Place of Presentation: 福岡市
  • Year and Date: 2008-05-15
• [Presentation] 褥瘡発生に関与する遺伝子の実験モデルによる解析 (2008)
  • Author(s): 黒瀬智之、橋本将和、小澤淳也、川真田聖一
  • Organizer: 第43回日本理学療法学術集会
  • Place of Presentation: 福岡市
  • Year and Date: 2008-05-15
• [Presentation] ラット褥瘡実験モデルによる発現遺伝子の解析 (2008)
  • Author(s): 黒瀬智之、橋本将和、小澤淳也、川真田聖一
  • Organizer: 第113回日本解剖学会総会・学術集会
  • Place of Presentation: 大分市
  • Year and Date: 2008-03-27
• [Presentation] ラット実験モデルによる褥瘡の組織変化 (2008)
  • Author(s): 橋本将和、黒瀬智之、小澤淳也、川真田聖一
  • Organizer: 第113回日本解剖学会総会・学術集会
  • Place of Presentation: 大分
  • Year and Date: 2008-03-27
• [Presentation] 褥瘡発生に関与する遺伝子の実験モデルによる解析 (2008)
  • Author(s): 黒瀬智之
  • Organizer: 第43回日本理学療法学術大会
  • Place of Presentation: 福岡市
• [Presentation] ラット褥瘡実験モデルの作製と褥瘡関連遺伝子の解析 (2008)
  • Author(s): 黒瀬智之
  • Organizer: 第10回日本褥瘡学会
  • Place of Presentation: 神戸市
• [Presentation] ラット実験モデルによる褥瘡の組織変化 (2008)
  • Author(s): 橋本 将和
  • Organizer: 第113回日本解剖学会総会・学術集会
  • Place of Presentation: 大分大学医学部
• [Presentation] ラット褥瘡実験モデルによる発現遺伝子の解析 (2008)
  • Author(s): 黒瀬 智之
  • Organizer: 第113回日本解剖学会総会・学術集会
  • Place of Presentation: 大分大学医学部
• [Presentation] 炭酸アンモニウム再注入による遺体および実習室のホルマリン減量 (2008)
  • Author(s): 川真田 聖一
  • Organizer: 第113回日本解剖学会総会・学術集会
  • Place of Presentation: 大分大学医学部
• [Presentation] Changes in hindlimb muscles induced by adjuvant-induced arthritis of the knee. (2007)
  • Author(s): Junya Ozawa
  • Organizer: World Physical Therapy 2007
  • Place of Presentation: Vancouver, Canada