Molecular mechanisms in light-driven anion pump of halorhodopsin
| Project/Area Number |
19614001
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
光生命科学
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| Research Institution | Hokkaido University |
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Principal Investigator |
KIKUKAWA Takashi Hokkaido University, 大学院・理学研究院, 助教 (20281842)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAUCHI Seiji 松山大学, 薬学部, 教授 (30202352)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ハロロドプシン / フォトサイクル / アニオンポンプ / 膜タンパク質 / レチナール |
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| Research Abstract |
Halorhodopsin in the cytoplasmic membrane of highly halophilic archaea functions as an inward-directed light-driven Cl- pump. To address the pump mechanism, we performed analyses using the wild-type and single amino acid mutants. The results can be summarized as follows : (1) acidic and aromatic residues in the vicinity of the active site play an essential roll in early step of the Cl- transport, (2) Cldependent equilibrium of photo-intermediates after flash excitation represents the Clreleasing process at the cytoplasmic side, (3) transient hydration of the cytoplasmic channel stabilizes the Cl- releasing state.
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Report
(4 results)
Research Products
(73 results)
