Molecular in vivo photo-imaging by Quantum dot conjugate multicolor luciferase probes

• Project/Area Number: 19614002
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 光生命科学
• Research Institution: Hokkaido University
• Principal Investigator: OHMIYA Yoshihiro Hokkaido University, 大学院・医学研究科, 教授 (20223951)
• Co-Investigator(Kenkyū-buntansha): YOSHINORI Yamanoi 東京大, 理学系, 助教 (20342636)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: in vivoイメージング / ホタルルシフェラーゼ / ウミホタルルシフェラーゼ / ナノドット / 近赤外蛍光体

Research Abstract

生体深部をin vivoイメージングすることを目標に、近赤外蛍光体・多色ルシフェラーゼを融合させ、2つの物質間に生じるエネルギー移動を利用、融合体より最大発光波長650-750nmの光を発する近赤外発光プローブの創製を目的とした。本研究の結果、最大発光波長460nm波長で励起できる近赤外蛍光体とルシフェラーゼ融合体の合成に成功した。

Research Products

• [Journal Article] A bioluminescent enzyme immunoassay for prostaglandin E2 using Cypridina luciferase (2009)
  • Author(s): Wu C, Irie S, Yamamoto S, Ohmiya Y
  • Journal Title: Luminescence 24 Pages: 131-133
  • Peer Reviewed
• [Journal Article] In vivo bioluminescence imaging of bone marrow-derived cells in brain inflammation (2009)
  • Author(s): Akimoto H, Kwon HJ, Ozaki M, Yasuda K, Honma K, Ohmiya Y
  • Journal Title: Biochem Biophys ResCommun 380 Pages: 844-849
  • Peer Reviewed
• [Journal Article] クラゲの発光システムを利用した1細胞生物発光イメージング (2009)
  • Author(s): 近江谷克裕、星野英人
  • Journal Title: 化学と工業 79巻 Pages: 122-124
• [Journal Article] クラゲの発光システムを利用した1細胞生物発光イメージング (2009)
  • Author(s): 近江谷克裕、星野英人
  • Journal Title: 化学と工業 79 Pages: 122-124
• [Journal Article] The structural origin and biological function of pH-sensitivity in firefly luciferases (2008)
  • Author(s): Viviani VR, Arnoldi FG, Neto AJ, Oehlmeyer AT, Bechhara FJ, Ohmiya Y
  • Journal Title: Photochem Photobiol Sci. 7 Pages: 159-619
  • Peer Reviewed
• [Journal Article] The influence of the loop between residues 223-235 in beetle luciferase bioluminescence spectra : a solvent gate for the active site of pH-sensitive luciferases (2008)
  • Author(s): Viviani VR, Neto AJ, Arnoldi FG, Barbosa JA, Ohmiya Y
  • Journal Title: Photochem Photobiol 84 Pages: 138-144
  • Peer Reviewed
• [Journal Article] Preparation of biotinylated Cypridina luciferase and its use in bioluminescent enzyme immunoassay (2008)
  • Author(s): Wu C, Kawasaki K, Ogawa Y, Yoshida Y, Ohgiya S, Ohmiya Y
  • Journal Title: Analyti Calhemistry 79 Pages: 1634-1638
  • Peer Reviewed
• [Journal Article] The structural origin and biological function of pH-sensitivity in firefly luciferases (2008)
  • Author(s): Viviani VR, Arnoldi FG, Neto AJ, Oehlmeyer AT, Bechhara FJ, Ohmiya Y.
  • Journal Title: Photochem Photobiol Sci 7 Pages: 159-619
  • Peer Reviewed
• [Journal Article] The influence of the loop between residues 223-235 in beetle luciferase bioluminescence spectra: a solvent gate for the active site of pH-sensitive luciferases. (2008)
  • Author(s): Viviani VR, Neto AJ, Arnoldi FG, Barbosa JA, Ohmiya Y
  • Journal Title: Photochem Photobiol 84 Pages: 138-144
  • Peer Reviewed
• [Journal Article] :Preparation of biotinylated Cypridina luciferase and its use in biolumi nescent enzyme immunoassay. (2007)
  • Author(s): Wu C, Kawasaki K, Ogawa Y, Yoshida Y, Ohgiya S, Ohmiya Y
  • Journal Title: Analytical Chemistry 79 Pages: 1634-1638
  • Peer Reviewed
• [Journal Article] In vivo bioluminescence imaging of bone marrow-derived cells in brain inflammation
  • Author(s): Akimoto H, Kwon HJ, Ozaki M, Yasuda K, Honma K, Ohmiya Y
  • Journal Title: Biochem Biophys Res Commun (in press)
  • Peer Reviewed
• [Journal Article] A bioluminescent enzyme immunoassay for prostaglandin E2 using Cypridina luciferase
  • Author(s): Wu C, Irie S, Yamamoto S, Ohmiya Y
  • Journal Title: Luminescence (in press)
  • Peer Reviewed
• [Presentation] ポストゲノム時代における発光イメージングの可能性 (2008)
  • Author(s): 近江谷克裕
  • Organizer: 第3回ケミカルバイオロジー研究会シンポジウム
  • Place of Presentation: 学術総合センター一橋記念講堂(東京)
  • Year and Date: 2008-05-20
• [Presentation] ポストゲノム時代における発光イメージングの可能性 (2008)
  • Author(s): 近江谷克裕
  • Organizer: 第3回ケミカルバイオロジー研究会シンポジウム
  • Place of Presentation: 東京・学術総合センター
  • Year and Date: 2008-05-19
• [Book] 発光生物のふしぎ (2009)
  • Author(s): 近江谷克裕
  • Total Pages: 218
  • Publisher: ソフトバンク クリェイティブ社
• [Book] 発光生物のふしぎ (2009)
  • Author(s): 近江谷克裕
  • Total Pages: 222
  • Publisher: ソフトバンククリエイティブ